##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031484_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1151268
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93665679928566	34.0	33.0	34.0	31.0	34.0
2	33.014624744195096	34.0	33.0	34.0	31.0	34.0
3	33.185973205196355	34.0	34.0	34.0	31.0	34.0
4	36.14077955784405	37.0	37.0	37.0	35.0	37.0
5	36.12538783324126	37.0	37.0	37.0	35.0	37.0
6	36.20390821251003	37.0	37.0	37.0	35.0	37.0
7	36.239312653526376	37.0	37.0	37.0	35.0	37.0
8	36.32599273149258	37.0	37.0	37.0	35.0	37.0
9	38.28209852093518	39.0	39.0	39.0	37.0	39.0
10-11	38.36777926599193	39.0	39.0	39.0	37.5	39.0
12-13	38.421349329608745	39.0	39.0	39.0	38.0	39.0
14-15	40.06882975988215	41.0	40.0	41.0	38.5	41.0
16-17	40.07625809107871	41.0	40.0	41.0	38.5	41.0
18-19	40.094974410823546	41.0	40.0	41.0	39.0	41.0
20-21	40.03186269400348	41.0	40.0	41.0	39.0	41.0
22-23	39.96820375446899	41.0	40.0	41.0	38.0	41.0
24-25	39.86202387280807	41.0	40.0	41.0	38.0	41.0
26-27	39.7782236629525	41.0	40.0	41.0	38.0	41.0
28-29	39.708852326304566	41.0	40.0	41.0	38.0	41.0
30-31	39.56475251635588	41.0	40.0	41.0	37.5	41.0
32-33	39.391602563434404	41.0	40.0	41.0	37.0	41.0
34-35	39.30812938429627	41.0	40.0	41.0	36.0	41.0
36-37	39.19666880344107	41.0	40.0	41.0	36.0	41.0
38-39	39.02080662365322	41.0	39.0	41.0	35.0	41.0
40-41	38.9079336870303	41.0	39.0	41.0	35.0	41.0
42-43	39.086983656281596	41.0	40.0	41.0	35.5	41.0
44-45	39.06933832956358	41.0	40.0	41.0	35.5	41.0
46-47	38.90913453687587	41.0	40.0	41.0	35.0	41.0
48-49	38.73118987064697	41.0	39.0	41.0	35.0	41.0
50-51	37.71158626835802	40.0	38.0	40.5	34.0	40.5
52-53	37.76727399701894	39.5	38.0	40.5	34.5	41.0
54-55	38.187357765524624	40.0	38.0	41.0	35.0	41.0
56-57	38.182991710010185	40.5	38.0	41.0	35.0	41.0
58-59	38.17963063335383	41.0	38.0	41.0	35.0	41.0
60-61	37.97638820847969	40.5	37.0	41.0	35.0	41.0
62-63	37.72488768905242	40.0	37.0	41.0	34.0	41.0
64-65	37.448578436992946	39.5	36.0	41.0	34.0	41.0
66-67	37.17954464121299	39.0	35.5	41.0	34.0	41.0
68-69	36.8564760768127	39.0	35.0	41.0	34.0	41.0
70-71	36.49394233141197	37.0	35.0	40.5	33.0	41.0
72-73	36.133749048874805	37.0	35.0	39.5	33.0	41.0
74-75	35.75428961805592	36.5	35.0	39.0	33.0	41.0
76-77	35.39292371541639	36.0	35.0	39.0	33.0	41.0
78-79	35.01375874253432	35.5	35.0	37.5	32.5	39.5
80-81	34.631168416042144	35.0	35.0	37.0	32.0	39.0
82-83	34.32375302709708	35.0	35.0	37.0	32.0	39.0
84-85	34.03760114934142	35.0	35.0	36.0	31.0	37.0
86-87	33.80819974150241	35.0	35.0	36.0	31.0	37.0
88-89	33.624231282377345	35.0	35.0	36.0	31.0	37.0
90-91	33.42703479989021	35.0	35.0	35.0	31.0	36.0
92-93	33.356167721156154	35.0	35.0	35.0	31.0	36.0
94-95	33.26405016034494	35.0	35.0	35.0	31.0	36.0
96-97	33.17635294301587	35.0	34.5	35.0	30.0	36.0
98-99	33.101753892230136	35.0	34.0	35.0	30.0	36.0
100-101	32.77056819089908	35.0	34.0	35.0	28.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12665410044554193
1101	2	0.07753314843188974
1101	3	0.1070422708097567
1101	4	0.0698106522938815
1101	5	-0.06379025376540426
1101	6	0.08325921872936703
1101	7	0.11195755860956069
1101	8	0.16089977441608738
1101	9	0.14771151483801503
1101	10-11	0.14079827429890202
1101	12-13	0.11568844031348391
1101	14-15	0.17650123966932796
1101	16-17	0.18589999127928536
1101	18-19	0.1866009646032296
1101	20-21	0.17426933878494566
1101	22-23	0.14813840750736773
1101	24-25	0.2221061732336409
1101	26-27	0.16506072278287576
1101	28-29	0.2719912200792933
1101	30-31	0.21839359391007207
1101	32-33	0.20540735135161725
1101	34-35	-0.01069898220730181
1101	36-37	0.1536324758164227
1101	38-39	0.03420762506600994
1101	40-41	0.14670686094007124
1101	42-43	0.1966635927000837
1101	44-45	0.16106718739134607
1101	46-47	0.21013714408842787
1101	48-49	0.1363567570206925
1101	50-51	0.02784489955553937
1101	52-53	0.015917580783529672
1101	54-55	0.13203163179355215
1101	56-57	0.26589052010200476
1101	58-59	0.32666674448301336
1101	60-61	0.16906104633162755
1101	62-63	0.26000492705123435
1101	64-65	0.29947526715996986
1101	66-67	0.20806129222912517
1101	68-69	0.037273384249459696
1101	70-71	0.22228968256828097
1101	72-73	0.14475232481417066
1101	74-75	0.016541336323378175
1101	76-77	0.05703979393991432
1101	78-79	0.16936099317728548
1101	80-81	0.10500485695423123
1101	82-83	-0.019806645497233433
1101	84-85	-0.08308595461480905
1101	86-87	0.06396324103229034
1101	88-89	-0.042312288214439775
1101	90-91	-0.03799058626471208
1101	92-93	0.06043621492292317
1101	94-95	0.07061485298361703
1101	96-97	0.03357929270303828
1101	98-99	-0.0959376969564758
1101	100-101	-0.2711963945932112
1102	1	0.09765053645663357
1102	2	0.10671935451140513
1102	3	0.09036161803705056
1102	4	-0.10782309601544426
1102	5	0.06748300841978505
1102	6	0.05133831756341323
1102	7	0.12370193866306778
1102	8	0.06156913299680866
1102	9	0.06525601533657976
1102	10-11	0.12003334947833366
1102	12-13	0.1172019015291923
1102	14-15	0.18209847930562972
1102	16-17	0.12518235595136673
1102	18-19	0.12602253216599024
1102	20-21	0.12914637623151037
1102	22-23	0.15162835231983962
1102	24-25	0.13713613640904754
1102	26-27	0.21732202931553957
1102	28-29	0.18360528253300856
1102	30-31	0.18411907791161752
1102	32-33	0.22053455909959752
1102	34-35	0.21727438166510638
1102	36-37	0.2698120969081259
1102	38-39	0.3464999313913566
1102	40-41	0.28757327552774825
1102	42-43	0.2853634771913036
1102	44-45	0.3432289786659908
1102	46-47	0.15703025403897186
1102	48-49	0.23181982876447904
1102	50-51	0.2396949158452486
1102	52-53	0.3234814314711585
1102	54-55	0.476208310866717
1102	56-57	0.44229020023153254
1102	58-59	0.30629079795271963
1102	60-61	0.42844786253445477
1102	62-63	0.31045511512322577
1102	64-65	0.351071164130083
1102	66-67	0.330481349292441
1102	68-69	0.4345268421084967
1102	70-71	0.3494841026055937
1102	72-73	0.27652365691673
1102	74-75	0.2170038650771673
1102	76-77	0.22618562130858777
1102	78-79	0.25919868853027594
1102	80-81	0.2518683492684559
1102	82-83	0.09510632801472951
1102	84-85	0.2089972511981415
1102	86-87	0.1942956496372048
1102	88-89	0.21747436607863335
1102	90-91	0.2340938042536891
1102	92-93	0.1634853505815741
1102	94-95	0.21115349906273195
1102	96-97	0.23083324418934836
1102	98-99	0.20884209591752523
1102	100-101	0.23902415807187793
1103	1	-0.028395012040711265
1103	2	-0.007499536690922071
1103	3	0.03303287959136725
1103	4	0.016944586187065624
1103	5	-0.020868799822594042
1103	6	0.01685487298909294
1103	7	-0.03142667209070993
1103	8	-0.03997150580372022
1103	9	0.021240199728232767
1103	10-11	-0.021451741450050577
1103	12-13	-0.05282820268937627
1103	14-15	0.03127947026299438
1103	16-17	0.03255031134635544
1103	18-19	0.028280916920259358
1103	20-21	-0.0031771995964575694
1103	22-23	-0.06389814943355532
1103	24-25	0.045007565440577935
1103	26-27	0.040369281003101776
1103	28-29	0.0719354930351912
1103	30-31	0.0673122469631906
1103	32-33	0.03544713561527857
1103	34-35	0.0026643629660014767
1103	36-37	-0.009363797740377322
1103	38-39	0.04049121272465328
1103	40-41	-3.568084192622223E-4
1103	42-43	-0.07020352979765221
1103	44-45	0.12331235731037538
1103	46-47	0.23801278070934018
1103	48-49	0.24281430997350384
1103	50-51	0.24321781039711965
1103	52-53	0.15034381029172295
1103	54-55	0.24010333069306
1103	56-57	0.30635610744081987
1103	58-59	0.3416684647526367
1103	60-61	0.2709998628830519
1103	62-63	0.38069371331621227
1103	64-65	0.32415071770100923
1103	66-67	0.1832695417699881
1103	68-69	0.16686832132636198
1103	70-71	0.05145091786724265
1103	72-73	0.04098372288456886
1103	74-75	-0.11395734803380009
1103	76-77	0.07263658212384172
1103	78-79	0.004089117889570559
1103	80-81	0.009423769259250037
1103	82-83	0.12011281680948827
1103	84-85	0.022457190231889967
1103	86-87	0.1399555458543844
1103	88-89	0.06009172800939666
1103	90-91	0.03428060576098346
1103	92-93	-0.0703352896588072
1103	94-95	-0.16185463040246617
1103	96-97	-0.03697162970068746
1103	98-99	-0.0771475068545655
1103	100-101	-0.15331915884843994
1104	1	-0.006344224512588426
1104	2	0.15766529062433676
1104	3	0.14056171990709032
1104	4	0.10513077140797122
1104	5	0.1491354860447629
1104	6	0.0776990102797086
1104	7	0.04183844452060015
1104	8	0.0200752815815477
1104	9	0.16832848859569083
1104	10-11	0.10699034576735045
1104	12-13	0.13879471241472885
1104	14-15	0.17516585906259508
1104	16-17	0.10597257642724855
1104	18-19	0.14804010474839657
1104	20-21	0.21664091890777826
1104	22-23	0.19360881510089456
1104	24-25	0.2467455739408777
1104	26-27	0.18445165894516435
1104	28-29	0.17207031927898697
1104	30-31	0.27105970222945075
1104	32-33	0.22104572842216186
1104	34-35	0.10033302970410318
1104	36-37	0.2870472347965034
1104	38-39	0.131415888605936
1104	40-41	0.117949648954216
1104	42-43	0.1362248247864457
1104	44-45	0.10037716319629908
1104	46-47	0.0848416853130729
1104	48-49	0.14969893085281427
1104	50-51	0.0847242034825868
1104	52-53	0.05480646967242109
1104	54-55	0.2378733403362645
1104	56-57	0.2718834477029688
1104	58-59	0.2762991658491032
1104	60-61	0.12810859890933557
1104	62-63	0.1862309646466187
1104	64-65	0.16328738329620762
1104	66-67	0.1651871995434746
1104	68-69	0.019110812129596866
1104	70-71	0.12503256364031046
1104	72-73	0.1373518967479157
1104	74-75	0.15736350271087218
1104	76-77	0.08472451383569535
1104	78-79	0.21153715174791188
1104	80-81	0.2416231335222605
1104	82-83	0.2264058987052593
1104	84-85	0.1099277248938435
1104	86-87	0.10122826988024514
1104	88-89	0.039927615005360906
1104	90-91	0.048602099385760766
1104	92-93	0.08069956872899553
1104	94-95	-0.0034266508317628563
1104	96-97	0.02663019536448985
1104	98-99	0.014231203099363654
1104	100-101	-0.04434919563546913
1105	1	0.04973109175690382
1105	2	0.009809712186232389
1105	3	0.02241259107834992
1105	4	-0.12170473576734508
1105	5	0.04549225764424847
1105	6	0.04268299249670093
1105	7	0.06406230121047685
1105	8	0.2052849837815387
1105	9	0.1533265272345048
1105	10-11	0.19875753219473324
1105	12-13	0.2028413274322176
1105	14-15	0.18593454920021912
1105	16-17	0.17101621671361045
1105	18-19	0.15966975072269918
1105	20-21	0.20815190253041038
1105	22-23	0.17136575341608307
1105	24-25	0.18768694558613674
1105	26-27	0.2235003170472254
1105	28-29	0.21783296188629464
1105	30-31	0.262216414988103
1105	32-33	0.13338350750751005
1105	34-35	0.07664550723016816
1105	36-37	0.14491678793304175
1105	38-39	0.1851179578419888
1105	40-41	0.03277222749038344
1105	42-43	0.06848962489105048
1105	44-45	0.08528980090019189
1105	46-47	0.12242586039725012
1105	48-49	0.19027418174359667
1105	50-51	0.05209900326030237
1105	52-53	0.06940390028926657
1105	54-55	0.13664871544659007
1105	56-57	0.05401871624600574
1105	58-59	-0.04222670777385673
1105	60-61	-0.02614224284150879
1105	62-63	-0.1564560184529853
1105	64-65	-0.06270678165426347
1105	66-67	-0.12932466669168718
1105	68-69	-0.16711838926950406
1105	70-71	-0.06067279087572075
1105	72-73	-0.12677521580152273
1105	74-75	-0.018387943792994577
1105	76-77	-0.12244589576190634
1105	78-79	-0.08595353178645837
1105	80-81	-0.1747460600313957
1105	82-83	-0.11610987390759675
1105	84-85	-0.1960213287493886
1105	86-87	-0.06571870798907042
1105	88-89	-0.12106172826553063
1105	90-91	-0.08663007674446987
1105	92-93	-0.06672696194996064
1105	94-95	-0.02831714149895248
1105	96-97	-0.14927407386502267
1105	98-99	-0.09364733960927651
1105	100-101	-0.07576202947548438
1106	1	0.12174112727518605
1106	2	0.2228453495760192
1106	3	0.19106793546521317
1106	4	0.07429327300991417
1106	5	0.03954302160800438
1106	6	0.06382293697456731
1106	7	0.19646487025428883
1106	8	0.21208476511351648
1106	9	0.2025749322712045
1106	10-11	0.09886846956588613
1106	12-13	-0.027231175064436286
1106	14-15	-0.14409916720935456
1106	16-17	-0.0019666777057807394
1106	18-19	0.08782768644773853
1106	20-21	0.11064803155675662
1106	22-23	0.05330220225967963
1106	24-25	-0.003669330583015551
1106	26-27	-0.025757555459549053
1106	28-29	0.06581631440906932
1106	30-31	-0.02446125124998133
1106	32-33	-0.022709878395730243
1106	34-35	0.09746554284007658
1106	36-37	0.09774664527965626
1106	38-39	0.030224039317197082
1106	40-41	0.0020823543239458786
1106	42-43	0.06956616785961245
1106	44-45	-0.0572849539381437
1106	46-47	-0.3884266089327255
1106	48-49	-0.06280427304312042
1106	50-51	-0.07670249137836294
1106	52-53	-0.007698336171479525
1106	54-55	0.03436988012865072
1106	56-57	-0.05156266276735977
1106	58-59	-0.015633424180371946
1106	60-61	-0.26009946209284607
1106	62-63	-0.2118739309063784
1106	64-65	-0.11127528490832361
1106	66-67	-0.056328563624532535
1106	68-69	-0.06365382967483413
1106	70-71	-0.2172540568902619
1106	72-73	-0.08621435982006886
1106	74-75	-0.2646040097843141
1106	76-77	-0.1476893013635916
1106	78-79	-0.22537653529553126
1106	80-81	-0.13586949751708488
1106	82-83	-0.13972892123577907
1106	84-85	-0.09659380528685801
1106	86-87	-0.21350324364019713
1106	88-89	-0.03604957325556768
1106	90-91	0.13145004248456615
1106	92-93	-0.05000656993562558
1106	94-95	0.04680723357167693
1106	96-97	-0.27772550116248595
1106	98-99	-0.10038336821936156
1106	100-101	-0.08103060022344266
1107	1	0.1504584965758511
1107	2	0.20592681745728925
1107	3	0.11304849239386527
1107	4	0.27727740784031596
1107	5	0.17198129103341842
1107	6	0.18264987951629053
1107	7	0.2009843250950496
1107	8	0.18258472980527785
1107	9	0.21044867000158973
1107	10-11	0.226604858239007
1107	12-13	0.23639486358631245
1107	14-15	0.2750052392591158
1107	16-17	0.15515058096239898
1107	18-19	0.17641312062141168
1107	20-21	0.21394817514360653
1107	22-23	0.1836881801802548
1107	24-25	0.22530755428857674
1107	26-27	0.2623900565264279
1107	28-29	0.20496903432056257
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2316	54-55	-0.5355317041741188
2316	56-57	-0.4645271468678587
2316	58-59	-0.499019550493351
2316	60-61	-0.4240111395234649
2316	62-63	-0.49415012118699053
2316	64-65	-0.5264958346398245
2316	66-67	-0.33573185749575174
2316	68-69	-0.30805563473269615
2316	70-71	-0.8946138260728489
2316	72-73	-0.4888996144071598
2316	74-75	-0.3038588843646295
2316	76-77	-0.2977296020012119
2316	78-79	-0.36734926434176174
2316	80-81	-0.396056831309032
2316	82-83	-0.32933649056828784
2316	84-85	-0.24497253887140147
2316	86-87	-0.17737102674576022
2316	88-89	-0.2391167854782026
2316	90-91	-0.22644323526640164
2316	92-93	-0.3810352372936521
2316	94-95	-0.34567579908981116
2316	96-97	-0.3639662987557415
2316	98-99	-0.51553717804142
2316	100-101	-0.387436348069798
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	432.0
3	129.0
4	140.0
5	163.0
6	148.0
7	259.0
8	513.0
9	469.0
10	400.0
11	464.0
12	564.0
13	596.0
14	751.0
15	834.0
16	1036.0
17	1349.0
18	1885.0
19	2571.0
20	3342.0
21	4523.0
22	5815.0
23	6204.0
24	5631.0
25	4651.0
26	4302.0
27	4342.0
28	4915.0
29	5610.0
30	6801.0
31	8286.0
32	10574.0
33	14322.0
34	22221.0
35	39284.0
36	87971.0
37	242491.0
38	453492.0
39	203482.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.182315890462384	19.532157034503374	13.6702157243067	23.615311350727538
2	14.805742751371408	21.468300912939966	40.813623084351555	22.912333251337074
3	17.269782009434323	27.210756657732908	31.526333719188106	23.99312761364466
4	12.054864290755361	18.06313165552415	40.22839551239931	29.65360854132118
5	12.538299418857399	38.81222242702678	35.758180686311675	12.891297467804144
6	28.68572372338437	38.803030237177346	19.515040355353115	12.996205684085169
7	25.640035787227717	33.71702875585477	23.964922256682065	16.67801320023545
8	25.030849194181652	34.86485052815386	22.010367416294113	18.093932861370373
9	26.8161457595076	15.488599929214253	21.096152959826913	36.59910135145124
10-11	23.497361329074838	26.30074546853956	30.56742615828375	19.634467044101854
12-13	24.820687027113227	24.490891070437726	29.639792424078493	21.048629478370557
14-15	22.38544147452936	25.73039999513081	27.361360729473983	24.522797800865845
16-17	21.490812781499113	28.718786596242552	27.80508365941785	21.985316962840486
18-19	21.571296254461664	27.935878899937094	29.443200025023547	21.049624820577698
20-21	22.79193800443409	27.13014176832646	29.11979034744992	20.95812987978953
22-23	22.39523883624111	27.1404068652977	28.836432361100123	21.62792193736107
24-25	21.99171045234859	27.178551600389156	28.988398462178395	21.84133948508386
26-27	21.921388677616477	27.38738793618084	28.800988928938793	21.890234457263894
28-29	22.045011333726492	27.216165390643894	28.95306114993398	21.785762125695637
30-31	22.399750831612543	27.212815815928593	28.75215270959746	21.6352806428614
32-33	22.163086071390797	27.402859514355242	28.620431656179285	21.813622758074676
34-35	22.322702418355853	27.324318832596482	28.677538194584944	21.675440554462718
36-37	22.41493937698353	27.17745535617164	28.57017920186294	21.837426064981887
38-39	22.328382168843355	27.41967778835992	28.495951025780762	21.755989017015963
40-41	22.390331454701425	27.258447318556	28.63109852116462	21.720122705577953
42-43	22.350955954459153	27.178934575019298	28.42469207068986	22.045417399831692
44-45	22.44758700511515	27.277444972711816	28.157003633063617	22.117964389109414
46-47	22.436883510451715	27.391315497905573	28.001844714506092	22.169956277136624
48-49	22.421685154229237	27.34126991029604	28.08993357001588	22.147111365458844
50-51	22.40152013002348	27.55805411875124	27.755942411462385	22.28448333976289
52-53	22.523871111441732	27.520610571458032	27.715416415981387	22.24010190111885
54-55	22.611410892619396	27.410227362771227	27.76497284551331	22.21338889909607
56-57	22.525853031450985	27.57565300037904	27.70259537759156	22.195898590578413
58-59	22.520377893573894	27.548824302106077	27.839160352491888	22.091637451828138
60-61	22.598592573434452	27.664701215758093	27.69430924893999	22.042396961867468
62-63	22.454056275891375	27.5863510367872	27.870160157518853	22.08943252980257
64-65	22.483765279320814	27.37833050759987	27.759837188975155	22.37806702410417
66-67	22.50715137871449	27.56419930871818	27.740346997899714	22.18830231466762
68-69	22.5430803335521	27.46059477629889	27.684928700694268	22.31139618945474
70-71	22.60003975449641	27.07990175332987	27.873077898370287	22.44698059380343
72-73	22.498289263097632	27.4465712830244	27.59130419273012	22.46383526114785
74-75	22.36203618708698	27.603160170711867	27.59337164634279	22.441431995858366
76-77	22.511838239580044	27.40834118489403	27.62883736873104	22.450983206794884
78-79	22.517705575053142	27.56586691499801	27.418652265769655	22.49777524417919
80-81	22.61053752125554	27.538338374264764	27.342769980207954	22.508354124271737
82-83	22.640846113499375	27.39378710546447	27.6055942886119	22.359772492424256
84-85	22.724692295368584	27.390326894812727	27.43612560339363	22.44885520642505
86-87	22.519726246760186	27.511338537355893	27.520655795661202	22.44827942022272
88-89	22.62131826499306	27.452672347624834	27.434256325772893	22.491753061609213
90-91	22.675968637395886	27.47269435298203	27.455680675464755	22.39565633415733
92-93	22.770640780889167	27.497402662944832	27.45228643972525	22.27967011644075
94-95	22.61529875077704	27.477902495618345	27.519359106033654	22.38743964757096
96-97	22.573398677118888	27.46309189535252	27.560950999119704	22.402558428408888
98-99	22.57165054422485	27.478069622152834	27.422520494887483	22.52775933873484
100-101	22.570106654100183	27.50758536004842	27.63673120042538	22.285576785426013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	252.0
2	180.0
3	190.0
4	174.5
5	174.0
6	228.5
7	347.5
8	597.0
9	730.0
10	620.0
11	535.5
12	553.0
13	610.5
14	669.0
15	726.5
16	782.0
17	840.0
18	907.0
19	1016.5
20	1144.0
21	1279.0
22	1506.5
23	1905.5
24	2398.5
25	3110.5
26	4570.5
27	6519.5
28	8538.0
29	11510.5
30	15643.0
31	19519.5
32	23516.0
33	27624.0
34	30705.0
35	33539.0
36	37125.5
37	39143.5
38	38593.0
39	38119.0
40	38189.0
41	37910.5
42	37232.5
43	37644.0
44	38847.0
45	39933.0
46	40304.0
47	40579.5
48	40902.0
49	40864.0
50	40388.0
51	38974.5
52	37184.0
53	35675.5
54	34574.0
55	32597.0
56	30177.0
57	27622.0
58	25813.0
59	23367.5
60	19166.0
61	15232.5
62	11713.5
63	8662.5
64	6224.5
65	4483.0
66	3206.5
67	2173.0
68	1511.0
69	1065.5
70	753.5
71	532.5
72	357.5
73	270.0
74	194.0
75	152.5
76	126.5
77	74.5
78	41.5
79	34.5
80	34.0
81	31.5
82	26.0
83	20.5
84	16.0
85	12.0
86	9.0
87	8.5
88	8.5
89	9.5
90	7.0
91	3.5
92	4.0
93	3.0
94	2.5
95	1.5
96	2.0
97	1.5
98	0.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04690480409426823
2	0.07027034539307964
3	0.050031791033886115
4	0.06253973879235765
5	0.09728403812144522
6	0.07496082580250646
7	0.09876067084293144
8	0.1145693270376663
9	0.1145693270376663
10-11	0.0680553963108503
12-13	0.0483380064415931
14-15	0.10240882227248564
16-17	0.0538970943342471
18-19	0.030574983409597068
20-21	0.03574319793479885
22-23	0.0834731791381329
24-25	0.18327617896093698
26-27	0.18640316590055486
28-29	0.13915091881299577
30-31	0.1602146502812551
32-33	0.21771646567089503
34-35	0.1841013560700028
36-37	0.18388420419919602
38-39	0.2242744521692603
40-41	0.16069238439703007
42-43	0.08720819131600982
44-45	0.07483053468002238
46-47	0.08290858427403523
48-49	0.12907507200756035
50-51	0.14153958939187053
52-53	0.1666423456571363
54-55	0.24659766448819911
56-57	0.20164722723119205
58-59	0.15096398058488555
60-61	0.2545888533338892
62-63	0.2571946757835708
64-65	0.2769554960269894
66-67	0.1445797155831657
68-69	0.1426687791200659
70-71	0.1487490315026562
72-73	0.16586059892223182
74-75	0.17050764895749732
76-77	0.1576522582057349
78-79	0.19678302532511976
80-81	0.2894200134112995
82-83	0.306270998585907
84-85	0.24008310836399516
86-87	0.24855203132546025
88-89	0.24420899390932435
90-91	0.1904421906975613
92-93	0.17493754712195597
94-95	0.16273361198261396
96-97	0.1438413992224226
98-99	0.15938947317218927
100-101	0.18931300096936596
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1151268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.67145806972157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.92628560486988	33.40581016719652
2	16.66143036752892	16.885179404996542
3	7.0964583218073765	10.787636708909675
4	3.651293788642216	7.400655204456753
5	2.0367255482581395	5.160192660904649
6	1.2881733426541964	3.9164172911301107
7	0.8308762779201353	2.9471198734429525
8	0.5593873272365563	2.2675977197440624
9	0.3964286412547568	1.807885554567939
>10	1.4903090569046389	12.00604288349197
>50	0.043323057853914444	1.4923011002542432
>100	0.018791470190707996	1.4274993523297106
>500	0.0	0.0
>1k	5.171948786296964E-4	0.49566207857494404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2562	0.22253723720280597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1994	0.1732003321555016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.686074832271895E-5	0.0
2	0.0	0.0	0.0	8.686074832271895E-5	0.0
3	0.0	0.0	0.0	1.737214966454379E-4	0.0
4	0.0	0.0	0.0	1.737214966454379E-4	0.0
5	0.0	0.0	0.0	1.737214966454379E-4	0.0
6	0.0	0.0	0.0	2.6058224496815685E-4	0.0
7	0.0	0.0	0.0	2.6058224496815685E-4	0.0
8	0.0	0.0	0.0	2.6058224496815685E-4	0.0
9	0.0	0.0	0.0	4.3430374161359473E-4	0.0
10-11	0.0	0.0	0.0	5.211644899363137E-4	0.0
12-13	0.0	0.0	0.0	6.080252382590326E-4	0.0
14-15	0.0	0.0	0.0	7.383163607431111E-4	0.0
16-17	0.0	0.0	0.0	0.0013029112248407841	0.0
18-19	0.0	0.0	0.0	0.0016937845922930196	0.0
20-21	0.0	0.0	0.0	0.002214949082229333	0.0
22-23	0.0	0.0	0.0	0.002909835068811085	0.0
24-25	0.0	0.0	0.0	0.003561290681231477	0.0
26-27	0.0	0.0	0.0	0.004039024797006432	0.0
28-29	0.0	0.0	0.0	0.005255075273524496	0.0
30-31	0.0	0.0	0.0	0.006992290239978875	0.0
32-33	0.0	0.0	0.0	0.011118175785308024	0.0
34-35	0.0	0.0	0.0	0.016199529562187086	0.0
36-37	0.0	0.0	0.0	0.02571078150352481	0.0
38-39	0.0	0.0	0.0	0.036568375043864676	0.0
40-41	0.0	0.0	0.0	0.04746939895836591	0.0
42-43	0.0	0.0	0.0	0.05706751164802635	0.0
44-45	0.0	0.0	0.0	0.0680553963108503	0.0
46-47	0.0	0.0	0.0	0.08095421743677406	0.0
48-49	0.0	0.0	0.0	0.09563368390331356	0.0
50-51	0.0	0.0	0.0	0.10922739101581908	0.0
52-53	0.0	0.0	0.0	0.12312511074745411	0.0
54-55	0.0	0.0	0.0	0.13628451411834602	0.0
56-57	0.0	0.0	0.0	0.1533526511637603	0.0
58-59	0.0	0.0	0.0	0.17098538307327227	0.0
60-61	0.0	0.0	0.0	0.18961701358849548	0.0
62-63	0.0	0.0	0.0	0.2075971884912983	0.0
64-65	0.0	0.0	0.0	0.22631567975484423	0.0
66-67	0.0	0.0	0.0	0.24698853785565134	0.0
68-69	0.0	0.0	0.0	0.26961576279371957	0.0
70-71	0.0	0.0	0.0	0.29120065875191525	0.0
72-73	0.0	0.0	0.0	0.3151307949148243	0.0
74-75	0.0	0.0	0.0	0.33836604509115165	0.0
76-77	0.0	0.0	0.0	0.3659443326836149	0.0
78-79	0.0	0.0	0.0	0.3947386707525963	0.0
80-81	0.0	0.0	0.0	0.42531365416219336	0.0
82-83	0.0	0.0	0.0	0.4601013838654423	0.0
84-85	0.0	0.0	0.0	0.49554056918111156	0.0
86-87	0.0	0.0	0.0	0.5331512732048489	0.0
88-89	0.0	0.0	0.0	0.5739323945423654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1550	0.0	52.35566	1
GTATCAA	3200	0.0	49.236515	1
ATCAACG	3845	0.0	40.366974	3
TCAACGC	3910	0.0	39.695915	4
CAACGCA	3940	0.0	39.6346	5
TATCAAC	3980	0.0	39.236263	2
AACGCAG	4030	0.0	38.848644	6
ACGCAGA	4725	0.0	33.1344	7
CGCAGAG	4800	0.0	32.320156	8
GTACATG	4660	0.0	28.311188	1
TACATGG	4745	0.0	27.80887	2
GCAGAGT	5520	0.0	27.761908	9
ACATGGG	4615	0.0	27.358025	3
TCGCGAA	35	0.008443973	27.109371	14-15
AGAGTAC	4855	0.0	23.404648	10-11
CATGGGG	2705	0.0	22.811344	4
GAGTACT	2880	0.0	22.486715	12-13
CAGAGTA	5245	0.0	21.754816	10-11
GTGTACG	140	4.8815386E-4	20.338665	1
CATGGGA	2085	0.0	19.577948	4
>>END_MODULE