FastQCFastQC Report
Fri 10 Feb 2017
SRR2031484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151268
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.2831660395320638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.20473078379664855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13520.11743573173231601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.062.048031
GTATCAA35150.044.5015221
TCAACGC42100.036.437864
ATCAACG42250.036.3084953
CAACGCA42500.035.983175
AACGCAG43600.035.1842656
TATCAAC44700.034.6371842
ACGCAGA50750.030.0401067
CGCAGAG51500.029.3259688
CCGCGCA650.00584809829.2266549
ACCGTAT1004.9578728E-528.4959878
GTACATG46450.027.2270261
TACATGG46550.026.6289022
ACATGGG45600.026.2463043
GCAGAGT57350.025.92059
GAGTACT31250.024.1672212-13
CAGAGTA56000.022.72894510-11
CATGGGA18950.021.5536944
AGAGTAC51050.021.11847510-11
GTACTTT37200.020.178914-15
Produced by FastQC (version 0.11.5)