FastQCFastQC Report
Fri 10 Feb 2017
SRR2031483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004303
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.17663991843099144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.1265554319762064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.053.6872441
GTATCAA21300.045.2465971
TCAACGC25650.036.4699364
ATCAACG25850.036.3714643
CAACGCA26100.035.6592065
TATCAAC26950.035.5899242
AACGCAG27600.033.7044036
GTACATG36500.029.0055771
ACGCAGA32000.028.7734137
GTATACG856.4259634E-427.9267641
TACATGG37950.027.90152
CGCAGAG33250.027.5489658
ACATGGG37200.027.316623
GACCCGT700.00842449527.1207456
AACCGTG1852.561028E-723.0892817
GCAGAGT39350.023.0371259
CATGGGG21700.022.3201224
GTACTAA1503.044821E-522.1552331
GTACCGT2005.427064E-721.3575866
GAACCGT1353.8164266E-421.093916
Produced by FastQC (version 0.11.5)