FastQCFastQC Report
Fri 10 Feb 2017
SRR2031483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004303
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.21447710501711134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.14607145453115244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.051.0618251
GTATCAA23250.047.2201581
TCAACGC27600.039.4059684
CAACGCA28400.038.295945
ATCAACG28650.038.127543
AACGCAG29750.036.7177856
TATCAAC30050.036.351212
TATCGCG300.00394749431.67347514-15
ACGCAGA33900.031.5223337
CGCAGAG34350.030.9711158
GTACATG40000.027.0902611
ACATGGG40500.025.4472163
TACATGG42200.025.3224452
GCAGAGT42550.024.3328239
TAGGTCG1252.25767E-422.7969535
CATGGGA18650.021.9005584
AGAGTAC36550.021.70024710-11
GTATACC1353.7989116E-421.1082923
CAGAGTA39350.020.27683310-11
TGAACCG1656.328203E-520.1488235
Produced by FastQC (version 0.11.5)