FastQCFastQC Report
Fri 10 Feb 2017
SRR2031482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364854
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.19928871512996993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.13964863641092745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.054.4735641
GTATCAA29050.047.5469671
TCAACGC36800.037.2852864
CAACGCA36950.037.0054325
ATCAACG37150.036.9340133
TATCAAC38750.035.6501162
AACGCAG39350.034.8588566
ACGCAGA43700.031.4975367
CGCAGAG45550.030.3224758
GTGCGCG1109.527517E-525.8891969
GCAGAGT53100.025.5641619
GTACATG43200.024.9412421
TACATGG43900.023.6820492
AGAGTAC45850.023.49712410-11
ACATGGG43200.023.4090233
TAACGTG1252.2634646E-422.789185
CAGAGTA49100.020.87855510-11
CATGGGG28550.020.2880924
AGTACTT31950.018.19673212-13
GAGTACT29550.018.06854612-13
Produced by FastQC (version 0.11.5)