FastQCFastQC Report
Fri 10 Feb 2017
SRR2031482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364854
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.23812070741632438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.17401128618885242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.10631173737264206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.060.365441
GTATCAA29650.045.064661
TCAACGC35800.036.88274
ATCAACG35900.036.647663
CAACGCA36100.036.3130575
TATCAAC36800.036.009522
AACGCAG37650.034.944256
ACGCAGA42650.030.5135357
CGCAGAG43650.029.814488
TACATGG40600.028.310682
GTACATG43000.027.4244351
ACATGGG42200.026.6745323
GCAGAGT50950.025.3562879
CATGGGG26400.024.6477684
GTATACG1252.2400508E-422.8242051
GTACCGT2005.391521E-721.3733926
AGAGTAC46500.021.34785710-11
GTATATA8500.021.257841
CAGAGTA49400.020.9118910-11
AGTACCG3252.1827873E-1120.465
Produced by FastQC (version 0.11.5)