##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1364854
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25050078616467	34.0	33.0	34.0	31.0	34.0
2	33.3596948831157	34.0	34.0	34.0	31.0	34.0
3	33.45257734526916	34.0	34.0	34.0	31.0	34.0
4	36.64184301031466	37.0	37.0	37.0	35.0	37.0
5	36.537855331046394	37.0	37.0	37.0	35.0	37.0
6	36.585526363992045	37.0	37.0	37.0	36.0	37.0
7	36.5973261608934	37.0	37.0	37.0	36.0	37.0
8	36.60269743137361	37.0	37.0	37.0	36.0	37.0
9	38.53850228669147	39.0	39.0	39.0	38.0	39.0
10-11	38.540882761086536	39.0	39.0	39.0	37.5	39.0
12-13	38.54359990152793	39.0	39.0	39.0	38.0	39.0
14-15	40.24948236221603	41.0	40.0	41.0	39.0	41.0
16-17	40.30005260635936	41.0	40.5	41.0	39.0	41.0
18-19	40.32036650073927	41.0	41.0	41.0	39.0	41.0
20-21	40.31145272681181	41.0	41.0	41.0	39.0	41.0
22-23	40.265133120465634	41.0	40.0	41.0	39.0	41.0
24-25	40.23927540967752	41.0	40.0	41.0	39.0	41.0
26-27	40.16430731785231	41.0	40.0	41.0	38.5	41.0
28-29	40.09168489816493	41.0	40.0	41.0	38.0	41.0
30-31	39.95915826894305	41.0	40.0	41.0	38.0	41.0
32-33	39.84488414145396	41.0	40.0	41.0	38.0	41.0
34-35	39.769681958656385	41.0	40.0	41.0	38.0	41.0
36-37	39.68486336267469	41.0	40.0	41.0	37.0	41.0
38-39	39.58640045015804	41.0	40.0	41.0	37.0	41.0
40-41	39.48579115421869	41.0	40.0	41.0	37.0	41.0
42-43	39.302600131589166	41.0	40.0	41.0	36.0	41.0
44-45	39.17939281417646	41.0	39.5	41.0	36.0	41.0
46-47	39.029420362910614	41.0	39.0	41.0	35.0	41.0
48-49	38.82716209938938	41.0	39.0	41.0	35.0	41.0
50-51	38.73200540131032	40.5	39.0	41.0	35.0	41.0
52-53	38.7521287991243	41.0	39.0	41.0	35.0	41.0
54-55	38.67980348081187	41.0	39.0	41.0	35.0	41.0
56-57	38.59768810436867	40.5	38.5	41.0	35.0	41.0
58-59	38.55885574574277	41.0	38.0	41.0	35.0	41.0
60-61	38.36831961513832	40.5	37.5	41.0	35.0	41.0
62-63	38.15617531252427	40.0	37.0	41.0	35.0	41.0
64-65	37.87977651822099	39.5	36.5	41.0	35.0	41.0
66-67	37.582391230124244	39.0	36.0	41.0	35.0	41.0
68-69	37.22716165978193	39.0	35.0	41.0	34.0	41.0
70-71	36.866396332501495	37.5	35.0	40.5	34.0	41.0
72-73	36.5146737306701	37.0	35.0	39.5	34.0	41.0
74-75	36.13243614335306	36.5	35.0	39.0	34.0	41.0
76-77	34.92756294812486	35.5	34.5	37.5	31.5	39.0
78-79	35.333318069185424	36.0	35.0	37.0	33.0	39.0
80-81	35.12092685371476	35.0	35.0	37.0	33.0	39.0
82-83	34.83235935858341	35.0	35.0	37.0	33.0	39.0
84-85	34.55993388303804	35.0	35.0	36.0	33.0	37.0
86-87	34.35265237160898	35.0	35.0	36.0	33.0	37.0
88-89	34.17652693987782	35.0	35.0	36.0	33.0	37.0
90-91	34.02878806084753	35.0	35.0	35.0	33.0	36.0
92-93	33.93270049397225	35.0	35.0	35.0	33.0	36.0
94-95	33.843813330949686	35.0	35.0	35.0	33.0	36.0
96-97	33.7699222774011	35.0	35.0	35.0	32.0	36.0
98-99	33.70862487855844	35.0	35.0	35.0	32.0	36.0
100-101	33.45018991042265	35.0	34.5	35.0	31.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06277205429378796
1101	2	0.11912435958885226
1101	3	0.052782716314908384
1101	4	0.08568534456266974
1101	5	0.12027639381946642
1101	6	0.09816160499300253
1101	7	0.08414827356162391
1101	8	0.11277874643971586
1101	9	0.10458004359985296
1101	10-11	0.1076677913210986
1101	12-13	0.16446242923949228
1101	14-15	0.1502618484460143
1101	16-17	0.09281878456734205
1101	18-19	0.10802426366127094
1101	20-21	0.10345568405625016
1101	22-23	0.1022266727316321
1101	24-25	0.12408099129959282
1101	26-27	0.08929702487415625
1101	28-29	0.1524777559989161
1101	30-31	0.0915609433161606
1101	32-33	0.07851756362291695
1101	34-35	0.09800829710405168
1101	36-37	0.09127566556088595
1101	38-39	0.12322988257743361
1101	40-41	0.15208498673150928
1101	42-43	0.26826127585994186
1101	44-45	0.2332739013951226
1101	46-47	0.24386080602090487
1101	48-49	0.24491088435367203
1101	50-51	0.2894178047300002
1101	52-53	0.22415122648745722
1101	54-55	0.28290410338090055
1101	56-57	0.3090658329054534
1101	58-59	0.3588994558400245
1101	60-61	0.376661780378889
1101	62-63	0.3126071676732707
1101	64-65	0.24891745056478953
1101	66-67	0.2825129323138569
1101	68-69	0.12383256790718633
1101	70-71	0.15997058281964627
1101	72-73	0.14333341827666146
1101	74-75	0.10418673743921403
1101	76-77	-0.041721626670735645
1101	78-79	0.08623222911246842
1101	80-81	0.10481031654733641
1101	82-83	0.13150260206941766
1101	84-85	0.15795875053964892
1101	86-87	0.15987248263410692
1101	88-89	0.23909887785959683
1101	90-91	0.17489002329524794
1101	92-93	0.06840806337684313
1101	94-95	0.1158499454529931
1101	96-97	0.09664295977657389
1101	98-99	0.1235369239783708
1101	100-101	0.12339268700509365
1102	1	0.1161045357693169
1102	2	0.11538236808269886
1102	3	0.10296538485565776
1102	4	0.08961350821829939
1102	5	0.13492285222064027
1102	6	0.09541573753383403
1102	7	0.09300828172297315
1102	8	0.12913098139858903
1102	9	0.12004469768375259
1102	10-11	0.10862819229881637
1102	12-13	0.13810429997418794
1102	14-15	0.11080640541812414
1102	16-17	0.08818234236520794
1102	18-19	0.11917192222018258
1102	20-21	0.08640341433608967
1102	22-23	0.06772059225306037
1102	24-25	0.09413921990250174
1102	26-27	0.012828703679794273
1102	28-29	0.06729025797398691
1102	30-31	0.042289213041819096
1102	32-33	0.17507736335389268
1102	34-35	0.13233198383471745
1102	36-37	0.1512303110025499
1102	38-39	0.13254398590778038
1102	40-41	0.22685337071603584
1102	42-43	0.20697938099843327
1102	44-45	0.1851336306417366
1102	46-47	0.13715256319746771
1102	48-49	0.19152138336373525
1102	50-51	0.1427031592333705
1102	52-53	0.2668595297653198
1102	54-55	0.08555750304922327
1102	56-57	0.20205085257375544
1102	58-59	0.14409595333047776
1102	60-61	0.11444586465236029
1102	62-63	0.16335103189263123
1102	64-65	0.02889185361169666
1102	66-67	0.11949036220389786
1102	68-69	0.1324332403252697
1102	70-71	0.10528234216707233
1102	72-73	0.16338676930665486
1102	74-75	0.07580166748383022
1102	76-77	-0.03557107591718278
1102	78-79	0.10318797784739786
1102	80-81	0.13860365309574263
1102	82-83	0.11905276893737238
1102	84-85	0.08975173260529345
1102	86-87	0.11015387050283465
1102	88-89	0.16484366950479057
1102	90-91	0.13611228811035403
1102	92-93	0.1629618753718276
1102	94-95	0.1286961672575302
1102	96-97	0.14754249414010445
1102	98-99	0.21089082005996573
1102	100-101	0.12389074902858255
1103	1	0.03582780838283384
1103	2	0.05343415073743074
1103	3	0.04392881291399675
1103	4	0.035770646756070335
1103	5	0.05964677273907171
1103	6	0.04424761079054207
1103	7	0.04775506870361568
1103	8	0.08022332290075695
1103	9	0.03352844966836699
1103	10-11	0.08872965813458933
1103	12-13	0.06996695460422586
1103	14-15	0.04883790014130085
1103	16-17	0.04032703851089536
1103	18-19	0.07791804951582293
1103	20-21	0.04023724439652199
1103	22-23	0.08554157678056384
1103	24-25	0.06100804599042675
1103	26-27	0.0642360316806787
1103	28-29	0.08749540168453507
1103	30-31	0.09337815140708727
1103	32-33	0.1088513374717266
1103	34-35	0.17167682762419645
1103	36-37	0.10602061886863368
1103	38-39	0.12799032694660184
1103	40-41	0.09625210186952415
1103	42-43	0.16988551593804857
1103	44-45	0.11640728080711682
1103	46-47	0.07015003309606271
1103	48-49	0.1287027483095926
1103	50-51	0.1440127124931152
1103	52-53	0.21359651047608708
1103	54-55	0.2434408042490901
1103	56-57	0.24797978460394887
1103	58-59	0.2682770735122588
1103	60-61	0.220342650127769
1103	62-63	0.17264977626599887
1103	64-65	0.19449929391609544
1103	66-67	0.1548990200459741
1103	68-69	0.24273970589271698
1103	70-71	0.22922164329363426
1103	72-73	0.19064164200970168
1103	74-75	0.19609175872716378
1103	76-77	0.041108797538477404
1103	78-79	0.14430801564839157
1103	80-81	0.17680255597676364
1103	82-83	0.1783612646068775
1103	84-85	0.178053318597982
1103	86-87	0.12920063876622834
1103	88-89	0.16972782541558473
1103	90-91	0.19340377071313242
1103	92-93	0.17739477532876435
1103	94-95	0.16671607822623002
1103	96-97	0.14155984490273
1103	98-99	0.12872404279134742
1103	100-101	0.06298245178813744
1104	1	0.10666534241916992
1104	2	0.051842849445783656
1104	3	0.01553530362340183
1104	4	0.047087310240542024
1104	5	0.0490974849634469
1104	6	0.004438931783440125
1104	7	0.0661587083264763
1104	8	0.04683700699735027
1104	9	0.04049381317460643
1104	10-11	0.08848430464923496
1104	12-13	0.09604891178928199
1104	14-15	0.1480099613416428
1104	16-17	0.0067871866594018115
1104	18-19	0.03798502159234829
1104	20-21	0.007861225434680819
1104	22-23	0.03320071603067731
1104	24-25	0.042408914061390135
1104	26-27	0.04083198465550453
1104	28-29	0.028045674522473973
1104	30-31	0.07605656017876328
1104	32-33	0.022622508710824718
1104	34-35	-0.10722076602665709
1104	36-37	0.049070335328785575
1104	38-39	0.03680031085724522
1104	40-41	-0.07135959629277977
1104	42-43	0.08909550811834777
1104	44-45	0.03003981364557262
1104	46-47	-0.023240259540408204
1104	48-49	-0.07690408048293307
1104	50-51	-0.04390616701673622
1104	52-53	0.025718675229576604
1104	54-55	-0.0021498257408296695
1104	56-57	0.02475614991856645
1104	58-59	0.09682332826883311
1104	60-61	0.009672479395796074
1104	62-63	-0.006141771169616561
1104	64-65	-0.08164559780610148
1104	66-67	0.05992046643365967
1104	68-69	-0.019379600963922883
1104	70-71	-0.01004817014042203
1104	72-73	-0.05988311649800693
1104	74-75	-0.024358803913678173
1104	76-77	-0.12415486472864501
1104	78-79	0.04091204370199364
1104	80-81	-0.01936804465586306
1104	82-83	-0.06470742342388291
1104	84-85	-0.030448498902806875
1104	86-87	0.057729806947982354
1104	88-89	0.01661204897364854
1104	90-91	0.019806298796524402
1104	92-93	-0.030130188704298178
1104	94-95	-0.12669355081875722
1104	96-97	-0.14489810205713383
1104	98-99	-0.04999210481233973
1104	100-101	-0.045837139520450876
1105	1	0.001768260634001706
1105	2	0.047829718678372046
1105	3	-0.007174550505830268
1105	4	-0.026772685350550773
1105	5	-0.009623750077970783
1105	6	-0.03138969662475688
1105	7	-0.019850331069257265
1105	8	-0.0661337377533684
1105	9	-0.03133762962214348
1105	10-11	-0.011287426051246996
1105	12-13	0.06133593030781981
1105	14-15	0.10268848255406482
1105	16-17	0.09652523378898792
1105	18-19	0.062020688027907056
1105	20-21	0.03209563521833303
1105	22-23	-0.005827223400572734
1105	24-25	-0.01558673642900743
1105	26-27	-0.10206476817516119
1105	28-29	-0.02874580829708151
1105	30-31	0.007707980337052334
1105	32-33	-0.004921149149076598
1105	34-35	0.012389320084039923
1105	36-37	-0.03463459476267161
1105	38-39	-0.004315575932139382
1105	40-41	-0.05490930097616342
1105	42-43	-0.06372756118710754
1105	44-45	-0.09317706446194052
1105	46-47	-0.10862251495759523
1105	48-49	-0.13866300480169969
1105	50-51	-0.1581904453899199
1105	52-53	-0.14640648510718535
1105	54-55	-0.12221079654496947
1105	56-57	-0.06563438076044292
1105	58-59	-0.05879556879696679
1105	60-61	-0.012515387893905938
1105	62-63	0.011578082948197732
1105	64-65	-0.18042018515666314
1105	66-67	-0.09966558772276812
1105	68-69	-0.11662877600770116
1105	70-71	-0.06836849187620686
1105	72-73	-0.11365432863826186
1105	74-75	-0.11560570140436965
1105	76-77	-0.26395596147211364
1105	78-79	-0.16670022630112413
1105	80-81	-0.0643345838145919
1105	82-83	-0.09232332563302492
1105	84-85	-0.004318317440990427
1105	86-87	-0.10891049386263774
1105	88-89	-0.16464245559001967
1105	90-91	-0.03729329332272613
1105	92-93	-0.05503849834643404
1105	94-95	-0.08388406230875489
1105	96-97	-0.037639515259400014
1105	98-99	-0.0013922174335689874
1105	100-101	-0.06066522081333403
1106	1	0.01679209553739014
1106	2	0.04802451017031473
1106	3	0.038323872224168554
1106	4	0.076057202157358
1106	5	0.028910335727680092
1106	6	-0.029738297643078226
1106	7	-0.01416025515468533
1106	8	0.09125345903473914
1106	9	0.08340429870541044
1106	10-11	0.1377453800825208
1106	12-13	0.13375318450530926
1106	14-15	0.09155533715845365
1106	16-17	0.06028048910111039
1106	18-19	0.11321811461586861
1106	20-21	0.07506230964058602
1106	22-23	0.12531885398576037
1106	24-25	0.14845583633373138
1106	26-27	0.15498289622073713
1106	28-29	0.16414781931569422
1106	30-31	0.19173480745418914
1106	32-33	0.06353047492499542
1106	34-35	0.23283918021292038
1106	36-37	0.3053163286031122
1106	38-39	0.18823148515122057
1106	40-41	0.1788591568704163
1106	42-43	0.2957593255578672
1106	44-45	0.20795867815357383
1106	46-47	-0.038626086134947
1106	48-49	0.22316002712787508
1106	50-51	0.17071093059553988
1106	52-53	-0.0484858842836573
1106	54-55	0.20188220557068348
1106	56-57	0.22606641950793716
1106	58-59	0.2283468400162718
1106	60-61	0.26416563562717243
1106	62-63	0.25638283153190145
1106	64-65	0.38409394534281915
1106	66-67	0.34101715645648056
1106	68-69	0.11237053681121267
1106	70-71	0.20085012369737143
1106	72-73	0.2995030250110986
1106	74-75	0.29570519122763983
1106	76-77	0.17903533243036662
1106	78-79	0.29854815990547934
1106	80-81	0.2880026435449565
1106	82-83	0.242351724282301
1106	84-85	0.21708223338605848
1106	86-87	0.2738033844900727
1106	88-89	0.0740918325823472
1106	90-91	0.3214955905761343
1106	92-93	0.36023342516543977
1106	94-95	0.35031374845131324
1106	96-97	0.2818775100580808
1106	98-99	0.3243732860753923
1106	100-101	0.3365984998123821
1107	1	0.04358372262917243
1107	2	0.03455418214919348
1107	3	0.03643844610107294
1107	4	-0.013290376384567537
1107	5	0.02170866912223346
1107	6	0.05381866657423018
1107	7	-0.010798707772394778
1107	8	0.02174614664591701
1107	9	-0.026741739171036727
1107	10-11	0.011250236642155187
1107	12-13	0.00736272865513854
1107	14-15	0.05487768572877627
1107	16-17	0.016143781276568347
1107	18-19	-0.021889485335854886
1107	20-21	-8.096933819743413E-4
1107	22-23	0.0343167392439625
1107	24-25	0.037236160314272126
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2316	24-25	-0.31293076653354746
2316	26-27	-0.26755050800080227
2316	28-29	-0.3200490948320365
2316	30-31	-0.6442943084000277
2316	32-33	-0.8354233702064349
2316	34-35	-0.7528576471706003
2316	36-37	-0.5430606268222391
2316	38-39	-0.5306712983185378
2316	40-41	-0.36987593168446153
2316	42-43	-0.3419596617745526
2316	44-45	-0.289731547765669
2316	46-47	-0.3105608172951193
2316	48-49	-0.3380135419087438
2316	50-51	-0.3668016686756417
2316	52-53	-0.27396945631087277
2316	54-55	-0.3713700493757983
2316	56-57	-0.3625320246436061
2316	58-59	-0.3298569931158397
2316	60-61	-0.6562448144760253
2316	62-63	-0.559809216435788
2316	64-65	-0.34609021631183623
2316	66-67	-0.3489898216013998
2316	68-69	-0.3469597542365719
2316	70-71	-0.4848983930038102
2316	72-73	-0.3609371095288125
2316	74-75	-0.35411186550877716
2316	76-77	-0.3199332810775246
2316	78-79	-0.34849078061120053
2316	80-81	-0.3006869953313185
2316	82-83	-0.2614341490271812
2316	84-85	-0.21133766109388574
2316	86-87	-0.23608219751317705
2316	88-89	-0.33380371069083736
2316	90-91	-0.3715032759509711
2316	92-93	-0.3147790356522151
2316	94-95	-0.3668301213802181
2316	96-97	-0.2811140461226671
2316	98-99	-0.5742754074771099
2316	100-101	-0.695783011657106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	10.0
14	18.0
15	82.0
16	204.0
17	481.0
18	986.0
19	1659.0
20	2595.0
21	3810.0
22	5452.0
23	6265.0
24	5728.0
25	4736.0
26	4079.0
27	4135.0
28	4764.0
29	5687.0
30	6935.0
31	8580.0
32	11112.0
33	14890.0
34	22745.0
35	39615.0
36	92074.0
37	285372.0
38	587520.0
39	244824.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29294990220115	19.152659343915314	13.655828730639167	23.89856202324437
2	14.77278055871423	20.99266731533475	40.748933214541104	23.485618911409915
3	17.454028049886656	26.795246964144148	30.847401993180224	24.903322992788972
4	11.873357882967701	17.983974842730433	40.077986363376596	30.064680910925272
5	12.805216690478808	38.44371176319742	35.69513133311353	13.055940213210244
6	29.26129827805758	38.711832913996666	19.16629910598496	12.860569701960795
7	26.17510737412207	33.27147079467841	23.433275647065546	17.12014618413398
8	25.222697812366746	35.32817429556568	21.423683412291716	18.02544447977586
9	26.406194362180862	15.67860005539054	20.529008963596105	37.38619661883249
10-11	23.6969302211079	26.304754940821507	30.093182127905255	19.905132710165336
12-13	25.103747360523542	24.127049486611753	29.39596469659026	21.373238456274443
14-15	22.281943709730125	25.29519640928627	27.02783594435742	25.39502393662619
16-17	21.797679458755294	28.368821866661197	27.49349014619879	22.340008528384722
18-19	21.674046317782455	27.70545483587573	29.165827098547837	21.45467174779398
20-21	22.837701099567777	26.866433325102633	28.93192380765367	21.363941767675918
22-23	22.456504505243785	26.79546676787407	28.633538825398176	22.11448990148397
24-25	22.09097090238223	26.982702911813277	28.81231252573535	22.114013660069133
26-27	22.086567875002288	27.220412873445316	28.598941256205006	22.094077995347387
28-29	22.100862070228757	27.04369844686684	28.771062692419846	22.08437679048455
30-31	22.41309326858404	26.96837903541331	28.64079234848563	21.977735347517026
32-33	22.224281864580387	27.100920684556733	28.541770768155423	22.133026682707456
34-35	22.395911943695076	27.113815836712206	28.513819060500246	21.976453159092475
36-37	22.450711160218404	26.96789008302975	28.467730354070742	22.113668402681103
38-39	22.370474521331627	27.246436913662382	28.367457291321692	22.015631273684303
40-41	22.521639677210896	27.1044375442355	28.40007063026522	21.97385214828839
42-43	22.487167125568007	27.006734786284838	28.25137340697247	22.25472468117469
44-45	22.526658529044134	27.129861508996566	28.205104721823727	22.138375240135574
46-47	22.48303249282662	27.164683108925846	27.932530823281805	22.419753574965732
48-49	22.4381589195423	27.281732729887775	27.948299750371657	22.331808600198265
50-51	22.592489746156	27.342558251651827	27.69871356203667	22.366238440155502
52-53	22.701842101792572	27.306070832484647	27.649221088849064	22.342865976873718
54-55	22.635498009310886	27.318379841360326	27.729815789820744	22.31630635950805
56-57	22.641689147703712	27.419196485484893	27.541370725366964	22.397743641444436
58-59	22.615349334067965	27.39978048934172	27.680689656183006	22.30418052040731
60-61	22.803251169950784	27.43638083500818	27.6449094043747	22.115458590666332
62-63	22.539435961786825	27.438929444846444	27.77064766388525	22.250986929481485
64-65	22.571957404059848	27.260134048394885	27.795697467119894	22.372211080425373
66-67	22.585845086108865	27.41386182721954	27.59249815355397	22.40779493311762
68-69	22.624474836864216	27.333944405611316	27.669887618386095	22.371693139138372
70-71	22.670975834565464	27.070386169241566	27.698664021183404	22.559973975009562
72-73	22.607078942499847	27.292303577937794	27.526363318306956	22.574254161255404
74-75	22.54717744934224	27.42657367763746	27.425694441137132	22.600554431883165
76-77	22.522307992996847	27.369893395691058	27.55174974456217	22.556048866749926
78-79	22.52120006975105	27.364282186959194	27.421724228379006	22.692793514910754
80-81	22.575967832456804	27.459054228510887	27.287314247531235	22.677663691501067
82-83	22.770201061798552	27.404579537445027	27.37300106824613	22.45221833251029
84-85	22.768259462184233	27.383551647282424	27.34402360985131	22.504165280682038
86-87	22.57603871494801	27.456986952754797	27.36642738474445	22.60054694755275
88-89	22.72058187807641	27.425114776872	27.288283746891533	22.566019598160054
90-91	22.63473488237143	27.433441257518243	27.45512867010002	22.47669519001031
92-93	22.71807094385187	27.41582616162608	27.350617721748993	22.515485172773058
94-95	22.73056671351299	27.394438232462303	27.41612725005239	22.458867803972314
96-97	22.677187450086237	27.301418320201282	27.423299488443455	22.59809474126903
98-99	22.71231941291889	27.239323766498103	27.307389654864185	22.74096716571882
100-101	22.613224564678713	27.410440970242973	27.453888840857704	22.522445624220612
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	23.5
2	35.5
3	57.0
4	50.5
5	62.0
6	115.0
7	214.5
8	407.0
9	567.5
10	533.5
11	455.5
12	497.0
13	582.5
14	671.0
15	765.5
16	833.5
17	942.5
18	1068.0
19	1149.0
20	1344.5
21	1601.5
22	1949.0
23	2582.0
24	3487.0
25	4628.0
26	6255.5
27	8321.0
28	10486.0
29	13406.5
30	16952.0
31	20191.0
32	23402.5
33	26811.0
34	30247.5
35	33370.0
36	36720.0
37	39518.5
38	41319.5
39	43106.5
40	44532.5
41	46039.5
42	46948.0
43	48424.5
44	50818.0
45	52442.5
46	53113.5
47	53530.5
48	54431.5
49	54146.5
50	52144.0
51	49442.0
52	47015.5
53	45506.0
54	43322.0
55	40079.5
56	36726.0
57	32898.0
58	29534.5
59	25794.0
60	21022.0
61	17007.5
62	13252.0
63	9486.0
64	6576.0
65	4614.5
66	3295.5
67	2327.0
68	1706.0
69	1299.5
70	926.5
71	622.5
72	410.0
73	232.5
74	155.5
75	115.0
76	68.0
77	33.5
78	19.5
79	13.0
80	9.5
81	7.0
82	6.0
83	6.0
84	5.0
85	2.5
86	1.0
87	0.0
88	1.5
89	1.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09825226727547415
2	4.3960745984552197E-4
3	0.0
4	0.0
5	2.9307163989701466E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.004762414148326488
20-21	0.0032970559488414144
22-23	0.0
24-25	0.0
26-27	0.002124769389253356
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0030772522189186535
38-39	0.0019049656593305951
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.019086290548293076
48-49	0.0018316977493563413
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.003883199228635444
62-63	0.010367409261356892
64-65	0.009011952926833199
66-67	0.005128753698197756
68-69	0.0029307163989701463
70-71	8.792149196910439E-4
72-73	0.0023079391641889903
74-75	0.0024544749841374975
76-77	0.0029673503539572733
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.762414148326488E-4
88-89	0.0023445731191761173
90-91	5.128753698197756E-4
92-93	0.0
94-95	0.007912934277219395
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1364854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.27920930444446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94287271780173	40.06272445757083
2	16.251078809165033	18.61697889066372
3	6.161554211886805	10.587868600300368
4	2.9514951220457233	6.762372274268152
5	1.5436604383245134	4.420982467089014
6	0.9112656743930618	3.131794637730955
7	0.5909516472103571	2.369447016255778
8	0.4054654776867877	1.857979356971845
9	0.2630225640076292	1.3559152046026168
>10	0.9441241898049322	8.594154251955768
>50	0.026171149256240707	1.0130486274051445
>100	0.007953179214182898	0.7068336140367454
>500	0.0	0.0
>1k	3.848192030456848E-4	0.5199006011490347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3250	0.23812070741632438	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2375	0.17401128618885242	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1451	0.10631173737264206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.326790997425365E-5	0.0
5	0.0	0.0	0.0	7.326790997425365E-5	0.0
6	0.0	0.0	0.0	7.326790997425365E-5	0.0
7	0.0	0.0	0.0	7.326790997425365E-5	0.0
8	0.0	0.0	0.0	7.326790997425365E-5	0.0
9	0.0	0.0	0.0	7.326790997425365E-5	0.0
10-11	0.0	0.0	0.0	1.0990186496138048E-4	0.0
12-13	0.0	0.0	0.0	1.465358199485073E-4	0.0
14-15	0.0	0.0	0.0	1.465358199485073E-4	0.0
16-17	0.0	0.0	0.0	2.1980372992276098E-4	0.0
18-19	0.0	0.0	0.0	2.1980372992276098E-4	7.326790997425365E-5
20-21	0.0	0.0	0.0	2.1980372992276098E-4	7.326790997425365E-5
22-23	0.0	0.0	0.0	3.663395498712683E-4	7.326790997425365E-5
24-25	0.0	0.0	0.0	6.594111897682828E-4	1.0990186496138048E-4
26-27	0.0	0.0	0.0	0.001062384694626678	1.465358199485073E-4
28-29	0.0	0.0	0.0	0.0016118940194335804	2.1980372992276098E-4
30-31	0.0	0.0	0.0	0.0023079391641889903	2.1980372992276098E-4
32-33	0.0	0.0	0.0	0.0039931010935968245	2.1980372992276098E-4
34-35	0.0	0.0	0.0	0.00622777234781156	3.663395498712683E-4
36-37	0.0	0.0	0.0	0.009414926431691596	3.663395498712683E-4
38-39	0.0	0.0	0.0	0.014470412219915098	3.663395498712683E-4
40-41	0.0	0.0	0.0	0.0200754073329455	4.0297350485839515E-4
42-43	0.0	0.0	0.0	0.025020991256207624	4.3960745984552197E-4
44-45	0.0	0.0	0.0	0.030772522189186538	5.861432797940292E-4
46-47	0.0	0.0	0.0	0.037220098266920854	5.861432797940292E-4
48-49	0.0	0.0	0.0	0.044070647849513575	6.59411189768283E-4
50-51	0.0	0.0	0.0	0.05026178624233801	6.59411189768283E-4
52-53	0.0	0.0	0.0	0.057002433959969345	7.693130547296634E-4
54-55	0.0	0.0	0.0	0.06359654585765218	8.059470097167902E-4
56-57	0.0	0.0	0.0	0.07242532900954973	8.059470097167902E-4
58-59	0.0	0.0	0.0	0.08249966663100962	8.059470097167902E-4
60-61	0.0	0.0	0.0	0.09140171769288144	8.792149196910439E-4
62-63	0.0	0.0	0.0	0.09990079524989486	9.524828296652976E-4
64-65	0.0	0.0	0.0	0.10957215936649634	9.524828296652976E-4
66-67	0.0	0.0	0.0	0.12078214959255715	9.524828296652976E-4
68-69	0.0	0.0	0.0	0.13246838123345062	9.524828296652976E-4
70-71	0.0	0.0	0.0	0.14485065801909947	9.524828296652976E-4
72-73	0.0	0.0	0.0	0.15701313107482556	0.0010257507396395512
74-75	0.0	0.0	0.0	0.16913897017556456	0.0010257507396395512
76-77	0.0	0.0	0.0	0.18294997120571138	0.0010257507396395512
78-79	0.0	0.0	0.0	0.19840950021027892	0.0010257507396395512
80-81	0.0	0.0	0.0	0.21621360233402254	0.0010257507396395512
82-83	0.0	0.0	0.0	0.23464048169254734	0.0010257507396395512
84-85	0.0	0.0	0.0	0.25050298420197326	0.0010257507396395512
86-87	0.0	0.0	0.0	0.2676476751359486	0.0010257507396395512
88-89	0.0	0.0	0.0	0.29010428954305734	0.0010257507396395512
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1410	0.0	60.36544	1
GTATCAA	2965	0.0	45.06466	1
TCAACGC	3580	0.0	36.8827	4
ATCAACG	3590	0.0	36.64766	3
CAACGCA	3610	0.0	36.313057	5
TATCAAC	3680	0.0	36.00952	2
AACGCAG	3765	0.0	34.94425	6
ACGCAGA	4265	0.0	30.513535	7
CGCAGAG	4365	0.0	29.81448	8
TACATGG	4060	0.0	28.31068	2
GTACATG	4300	0.0	27.424435	1
ACATGGG	4220	0.0	26.674532	3
GCAGAGT	5095	0.0	25.356287	9
CATGGGG	2640	0.0	24.647768	4
GTATACG	125	2.2400508E-4	22.824205	1
GTACCGT	200	5.391521E-7	21.373392	6
AGAGTAC	4650	0.0	21.347857	10-11
GTATATA	850	0.0	21.25784	1
CAGAGTA	4940	0.0	20.91189	10-11
AGTACCG	325	2.1827873E-11	20.46	5
>>END_MODULE