FastQCFastQC Report
Fri 10 Feb 2017
SRR2031481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381723
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.1624059236185545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.11999510755773769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.053.367421
GTATCAA26100.044.3664971
TCAACGC30800.036.8352134
ATCAACG30800.036.681093
CAACGCA31350.036.1889845
AACGCAG33250.034.392866
TATCAAC33400.034.1074522
ACGCAGA37700.030.207367
CGCAGAG39900.028.5417948
TACATGG38150.024.3863072
GTACATG40250.024.1709561
ACATGGG38450.023.7039593
GCAGAGT48450.023.700889
AGAGTAC42200.020.46453310-11
GTATAGT4750.019.9821511
GAGTACT26200.019.83150712-13
CAGAGTA45350.019.40928310-11
GTATAGG4550.018.7744391
TATACAG9250.017.9614895
GTACTTT30150.017.70389414-15
Produced by FastQC (version 0.11.5)