FastQCFastQC Report
Fri 10 Feb 2017
SRR2031481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381723
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.2042377524293943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.15191177971272102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.053.790931
GTATCAA30500.043.642871
TCAACGC34300.038.077564
ATCAACG34850.037.6129073
CAACGCA35250.037.0513575
TATATCG651.3294583E-436.533164
AACGCAG36700.035.5874756
TATCAAC38800.033.9061622
ACGCAGA42900.030.5550047
CGCAGAG43700.029.7782848
GTACATG39450.024.9447081
TACATGG36250.024.7619252
GCAGAGT51600.024.574929
ACATGGG37150.024.0341953
CAGAGTA48150.021.65054110-11
AGAGTAC45200.021.64508810-11
GAGTACT31100.021.30433512-13
CATGGGG25450.019.5944064
ATCTAAC2803.4566256E-818.6762521
ATATCGG1300.00757021318.266585
Produced by FastQC (version 0.11.5)