FastQCFastQC Report
Fri 10 Feb 2017
SRR2031478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.2006188523645097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.13673511393596424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.055.8250771
GTATCAA24000.048.4606861
ATCAACG28050.040.2858963
CAACGCA28250.040.000695
TCAACGC28250.040.000694
AACGCAG29500.038.450256
TATCAAC30750.036.9014322
ACGCAGA34350.033.021327
CGCAGAG35100.032.045318
GTACATG31400.028.7249071
TACATGG30350.028.1581572
GCAGAGT40300.027.9104339
ACATGGG31550.027.0883223
AGAGTAC35600.023.1945210-11
CATGGGG19200.022.9980354
CAGAGTA39050.021.93523410-11
GAGTACT24850.021.86579512-13
CATGGGA13250.020.7836174
GTACTTT27750.019.23869914-15
GTATAGC7000.018.9886761
Produced by FastQC (version 0.11.5)