FastQCFastQC Report
Fri 10 Feb 2017
SRR2031478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.23298775191587162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.17363053506047177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.10573271146328776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.063.3867071
GTATCAA25750.043.3860441
ATCAACG31850.034.8945543
CAACGCA32300.034.408415
TCAACGC32350.034.3552254
AACGCAG33450.033.083476
TATCAAC34050.032.639992
ACGCAGA38250.028.8076487
CGCAGAG38800.028.276888
TACATGG32050.027.5636162
GTACATG34600.026.5179941
ACATGGG32950.026.2341613
GCAGAGT46050.023.5156219
TACACCG1503.035561E-522.1645055
CAGAGTA42400.021.78737810-11
GAGTACT27450.021.19692412-13
AGAGTAC39300.020.90762910-11
CATGGGG21450.020.3709744
GACCGTT1200.004766239319.7897367
TCTACAC5400.019.3499643
Produced by FastQC (version 0.11.5)