FastQCFastQC Report
Fri 10 Feb 2017
SRR2031477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128368
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.16740992300384272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.11760347687988315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.052.3020171
GTATCAA23450.043.923641
TCAACGC27600.035.777734
CAACGCA28400.034.7699055
ATCAACG29100.034.2597963
TATCAAC30100.033.749492
AACGCAG29850.033.388636
GTACATG37100.030.8336241
TACATGG36700.030.5257192
ACGCAGA33750.029.671017
ACATGGG37250.029.4404343
CGCAGAG35300.027.8303878
CATGGGG24800.026.6085744
GCAGAGT39850.023.7000039
GAGTACT22650.021.5810912-13
CAGAGTA37400.020.87366310-11
CATGGGA15050.020.5038474
TATACAG7350.020.023175
AGAGTAC36500.019.82811210-11
GTGGTAT5750.019.8118421
Produced by FastQC (version 0.11.5)