FastQCFastQC Report
Fri 10 Feb 2017
SRR2031477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128368
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.1962125831289083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17410.15429363470073593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13400.057.465531
GTATCAA25950.043.411841
TCAACGC29800.037.454354
CAACGCA30450.036.6548355
ATCAACG30500.036.594743
AACGCAG31800.035.248096
TATCAAC32100.034.9186672
ACGCAGA36700.030.283117
CGCAGAG37500.029.8903778
GCAGAGT43650.025.352599
TACATGG35400.023.7476522
GTACATG38200.022.7711471
GAGTACT23700.022.64744812-13
ACATGGG35650.021.5827183
CAGAGTA41550.021.43289810-11
GTACTTT25200.020.92518614-15
AGAGTAC38900.020.87839710-11
GAGTACA26250.020.0997581
CATGGGG23600.019.3201244
GTATAAC3003.6834535E-919.0132871
Produced by FastQC (version 0.11.5)