##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031477_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1128368 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86338853990897 34.0 31.0 34.0 31.0 34.0 2 33.020449888688795 34.0 33.0 34.0 31.0 34.0 3 33.132743041277315 34.0 33.0 34.0 31.0 34.0 4 36.476918877529314 37.0 37.0 37.0 35.0 37.0 5 36.30078573656821 37.0 37.0 37.0 35.0 37.0 6 36.389788615061754 37.0 37.0 37.0 35.0 37.0 7 36.39935375693036 37.0 37.0 37.0 35.0 37.0 8 36.403007706705615 37.0 37.0 37.0 35.0 37.0 9 38.27119078173078 39.0 39.0 39.0 37.0 39.0 10-11 38.246479428697015 39.0 39.0 39.0 37.0 39.0 12-13 38.25540913957148 39.0 39.0 39.0 37.0 39.0 14-15 39.84059234221459 41.0 40.0 41.0 38.0 41.0 16-17 39.883543312110945 41.0 40.0 41.0 38.0 41.0 18-19 39.88510796123251 41.0 40.0 41.0 38.0 41.0 20-21 39.86492793131319 41.0 40.0 41.0 38.0 41.0 22-23 39.80459433447244 41.0 40.0 41.0 38.0 41.0 24-25 39.75277258837542 41.0 40.0 41.0 37.5 41.0 26-27 39.64823577059966 41.0 40.0 41.0 37.0 41.0 28-29 39.54435964153539 41.0 40.0 41.0 37.0 41.0 30-31 39.38585461480652 41.0 39.0 41.0 36.5 41.0 32-33 39.24307539738808 40.5 39.0 41.0 36.0 41.0 34-35 39.13694246912355 40.0 39.0 41.0 35.0 41.0 36-37 39.02646654282999 40.0 39.0 41.0 35.0 41.0 38-39 38.90602356677963 40.0 38.0 41.0 35.0 41.0 40-41 38.7768914928463 40.0 38.0 41.0 35.0 41.0 42-43 38.56269895991379 40.0 38.0 41.0 35.0 41.0 44-45 38.42322185669923 40.0 38.0 41.0 34.5 41.0 46-47 38.2447437360861 40.0 38.0 41.0 34.0 41.0 48-49 38.01848377479688 40.0 38.0 41.0 33.5 41.0 50-51 37.90189459467124 40.0 38.0 41.0 33.0 41.0 52-53 37.934999929101146 40.0 38.0 41.0 33.5 41.0 54-55 37.83990550955008 40.0 37.0 41.0 33.0 41.0 56-57 37.779084483076446 40.0 37.0 41.0 33.0 41.0 58-59 37.77408921557506 40.0 37.0 41.0 34.0 41.0 60-61 37.57035692256427 39.5 36.0 41.0 33.0 41.0 62-63 37.35440033747855 39.0 36.0 41.0 33.0 41.0 64-65 37.060090325142156 39.0 35.0 41.0 33.0 41.0 66-67 36.76034635863478 38.5 35.0 40.5 32.5 41.0 68-69 36.40233992810856 37.5 35.0 40.0 32.0 41.0 70-71 36.057464408774436 37.0 35.0 39.5 32.0 41.0 72-73 35.733394601761134 36.5 35.0 39.0 32.0 41.0 74-75 35.36182123208032 36.0 35.0 39.0 31.0 40.5 76-77 34.035277055003334 35.0 33.5 37.0 29.5 39.0 78-79 34.646181476255975 35.0 34.5 37.0 31.0 39.0 80-81 34.512264172681256 35.0 35.0 37.0 31.0 39.0 82-83 34.23410846461438 35.0 34.5 36.0 31.0 38.0 84-85 33.9708836124385 35.0 34.0 36.0 31.0 37.0 86-87 33.76278705174198 35.0 34.0 36.0 31.0 37.0 88-89 33.579839644513136 35.0 34.0 35.0 30.0 36.5 90-91 33.4217533641507 35.0 34.0 35.0 30.0 36.0 92-93 33.31310352650908 35.0 34.0 35.0 30.0 36.0 94-95 33.212284467478696 35.0 34.0 35.0 30.0 36.0 96-97 33.12322265431136 35.0 34.0 35.0 29.5 35.5 98-99 33.038416988074815 35.0 34.0 35.0 29.0 35.0 100-101 32.66849866355657 35.0 33.5 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08831229773576865 1101 2 -0.13531273059472682 1101 3 -0.019650163773725637 1101 4 0.023854587308669295 1101 5 -0.03760065266595092 1101 6 0.06941197242193908 1101 7 0.04646451182490807 1101 8 -0.03869440825260284 1101 9 0.031341696680215136 1101 10-11 -0.011315740233719396 1101 12-13 0.038747861125365546 1101 14-15 0.05309329742301827 1101 16-17 -0.01106226006417188 1101 18-19 -0.0932959208302293 1101 20-21 0.06419534579021757 1101 22-23 -0.01986944227368781 1101 24-25 -0.14287033532045967 1101 26-27 -0.1114750524975534 1101 28-29 -0.049276769838648704 1101 30-31 -0.0122078598352644 1101 32-33 -0.0340463696493174 1101 34-35 -0.1293319540803921 1101 36-37 -0.14160354275454523 1101 38-39 -0.20076933778582173 1101 40-41 -0.11058562093737834 1101 42-43 -0.09039131235132203 1101 44-45 -0.02350246337288553 1101 46-47 0.05134244270705324 1101 48-49 0.12552689522507876 1101 50-51 0.1749146377939539 1101 52-53 0.04473287449683028 1101 54-55 0.056500468285491934 1101 56-57 0.022556229633607927 1101 58-59 0.1186264222449509 1101 60-61 0.09109963355985684 1101 62-63 0.07360513624539067 1101 64-65 0.05739061487104635 1101 66-67 0.009825255773627362 1101 68-69 0.05808465048162503 1101 70-71 0.041450748968571816 1101 72-73 -0.06445139793635946 1101 74-75 0.05811507839073471 1101 76-77 -0.0067714368605891195 1101 78-79 0.09158441753005775 1101 80-81 0.1482752951810511 1101 82-83 0.1483355367241188 1101 84-85 0.19829052554754156 1101 86-87 0.13110645678712984 1101 88-89 0.040895540890396376 1101 90-91 0.22707317667892113 1101 92-93 0.09802028680670816 1101 94-95 0.06793345168990328 1101 96-97 0.11540693584322526 1101 98-99 0.03281466434260949 1101 100-101 -0.019992961054612124 1102 1 0.154538156907563 1102 2 0.11016701046698785 1102 3 0.1895766363787743 1102 4 0.031566634023448614 1102 5 0.18560620757888557 1102 6 0.1537447045685738 1102 7 0.036518263052137456 1102 8 0.07443602292271123 1102 9 0.14170159877831168 1102 10-11 0.1507118129495737 1102 12-13 0.19617268151014144 1102 14-15 0.22081105310680016 1102 16-17 0.11950472550142166 1102 18-19 0.14528959814270337 1102 20-21 0.19033096639861213 1102 22-23 0.11561189887967771 1102 24-25 0.1386415932640901 1102 26-27 0.01770222383037634 1102 28-29 0.14451803519710182 1102 30-31 0.11013988946790931 1102 32-33 0.22463264603750588 1102 34-35 0.051419140797143825 1102 36-37 -0.1239580875087185 1102 38-39 0.002600336723929786 1102 40-41 0.13240311323208687 1102 42-43 0.24748582036344402 1102 44-45 0.2944357493840002 1102 46-47 0.07792717546211492 1102 48-49 0.20553987374631788 1102 50-51 0.20698264761575302 1102 52-53 0.36316494270419497 1102 54-55 0.3685464916626344 1102 56-57 0.3153399150200258 1102 58-59 0.3201233968864656 1102 60-61 0.23013426467946374 1102 62-63 0.19513506092255284 1102 64-65 0.3317026248429755 1102 66-67 0.2947739244381111 1102 68-69 0.3924450385334879 1102 70-71 0.327498739865824 1102 72-73 0.3772524231220302 1102 74-75 0.34938213368871374 1102 76-77 0.19299309286062538 1102 78-79 0.43482185730438516 1102 80-81 0.36512426035634604 1102 82-83 0.38792506382788616 1102 84-85 0.3847560218426409 1102 86-87 0.3516623009737643 1102 88-89 0.22633776869208333 1102 90-91 0.23793058875783402 1102 92-93 0.26467329013924257 1102 94-95 0.3375074124698827 1102 96-97 0.48742676512727456 1102 98-99 0.3216697296204245 1102 100-101 0.46295519795103957 1103 1 0.1402656155515345 1103 2 0.1776299014768412 1103 3 0.15184302526607496 1103 4 0.11916183372041189 1103 5 0.06850651501100913 1103 6 0.10288831382558783 1103 7 0.11398561154089748 1103 8 0.22758581525776123 1103 9 0.17735856038468967 1103 10-11 0.168042892673391 1103 12-13 0.19397789924746434 1103 14-15 0.27553712859315027 1103 16-17 0.12635403401827006 1103 18-19 0.11663068710671354 1103 20-21 0.12715002910366735 1103 22-23 0.16109301007369226 1103 24-25 0.24873175677512904 1103 26-27 0.18421823536242954 1103 28-29 0.12549435699263967 1103 30-31 0.19147118345681235 1103 32-33 0.1376687630951139 1103 34-35 0.1828956376395965 1103 36-37 0.19706648219151646 1103 38-39 0.07514484933782484 1103 40-41 0.17605818854052302 1103 42-43 0.16708045761457413 1103 44-45 0.16912559111479908 1103 46-47 0.12596272816080045 1103 48-49 -0.047433532513665 1103 50-51 0.0946603967610784 1103 52-53 0.1900899327083394 1103 54-55 0.24365625468928442 1103 56-57 0.27483090153854306 1103 58-59 0.36134646787898816 1103 60-61 0.42856955566866617 1103 62-63 0.27266554108678065 1103 64-65 0.30145537824490987 1103 66-67 0.2640020153553735 1103 68-69 0.2714187470602809 1103 70-71 0.19485000259499685 1103 72-73 0.19055388452466104 1103 74-75 0.1273193348893642 1103 76-77 0.030887689745746627 1103 78-79 0.20016811541345447 1103 80-81 0.18199266142231352 1103 82-83 0.11344719148013382 1103 84-85 0.17689354558875436 1103 86-87 0.11948504253373216 1103 88-89 0.2518424873065541 1103 90-91 0.20480750303458706 1103 92-93 0.1654178612934274 1103 94-95 0.15783370757794302 1103 96-97 0.16246603111632396 1103 98-99 0.24971492424772634 1103 100-101 0.10795909107103085 1104 1 0.07762017974274471 1104 2 0.09870673590717871 1104 3 0.029942885647692208 1104 4 0.027634231389008335 1104 5 0.026449249315028567 1104 6 -0.03588792002410912 1104 7 0.06888111937504249 1104 8 0.11090474352050705 1104 9 0.07093833123325766 1104 10-11 0.05015401117172047 1104 12-13 0.1012092446810513 1104 14-15 0.17610335867536975 1104 16-17 0.0905075699090574 1104 18-19 0.048804966299144326 1104 20-21 -0.02233028027292505 1104 22-23 0.10664184326297743 1104 24-25 0.09367779316455227 1104 26-27 0.15741649345697084 1104 28-29 0.14210728166519715 1104 30-31 0.19719719901429045 1104 32-33 0.17116332241075582 1104 34-35 0.04364015817235867 1104 36-37 0.019427030027436842 1104 38-39 0.14871043673228002 1104 40-41 0.17556509189358138 1104 42-43 0.29805579170140106 1104 44-45 0.31816820796208134 1104 46-47 0.29467657697404803 1104 48-49 0.42486440866932185 1104 50-51 0.5027034125180094 1104 52-53 0.40383624833452103 1104 54-55 0.3226736072589276 1104 56-57 0.4424228169697031 1104 58-59 0.3659976435543584 1104 60-61 0.28262956940888273 1104 62-63 0.23631766971297452 1104 64-65 0.07612960203321961 1104 66-67 0.13989402164928322 1104 68-69 0.1334763451809735 1104 70-71 0.14275074057784565 1104 72-73 0.233198433867571 1104 74-75 0.2815727090001232 1104 76-77 0.22289342961232705 1104 78-79 0.3674192955211666 1104 80-81 0.43450800836289716 1104 82-83 0.36561072227374325 1104 84-85 0.33733832984512446 1104 86-87 0.4461394094862783 1104 88-89 0.3080250327933314 1104 90-91 0.4247950925122339 1104 92-93 0.5216607972684244 1104 94-95 0.3802479974079631 1104 96-97 0.256229189594265 1104 98-99 0.3022601894118324 1104 100-101 0.3589258929237431 1105 1 0.017704561386203466 1105 2 0.04583383209673997 1105 3 0.01119389777949209 1105 4 -0.023685588477370345 1105 5 -0.10161772538426561 1105 6 -0.0533103691935608 1105 7 -0.036965470731395556 1105 8 -0.10065233726741951 1105 9 -0.009699716653067014 1105 10-11 0.008299992448577598 1105 12-13 0.02081841537040674 1105 14-15 0.18822998971499771 1105 16-17 0.0123597969435707 1105 18-19 0.011346614445947978 1105 20-21 0.0769680324387636 1105 22-23 0.09344622876545827 1105 24-25 0.14416895703859467 1105 26-27 0.025037837292352094 1105 28-29 0.05783804943823867 1105 30-31 0.09491632770232883 1105 32-33 0.09040769495795331 1105 34-35 0.24638307841809848 1105 36-37 0.17064339710308474 1105 38-39 0.09146512354855929 1105 40-41 -0.015327272411845172 1105 42-43 0.17189937728382887 1105 44-45 0.16277884301234025 1105 46-47 0.0039160912886018195 1105 48-49 0.02313508458385627 1105 50-51 0.11169105675756441 1105 52-53 0.17478306963770507 1105 54-55 0.11153946835481321 1105 56-57 0.03660534180354347 1105 58-59 0.19033764748229487 1105 60-61 0.24869860455009984 1105 62-63 0.03798419855743873 1105 64-65 0.12319889903662329 1105 66-67 0.1619124105766403 1105 68-69 0.1251609287339548 1105 70-71 0.003039943241944343 1105 72-73 0.10139820025894153 1105 74-75 0.051076704121221894 1105 76-77 -0.09977203455562034 1105 78-79 0.08496948864452492 1105 80-81 0.11207727263352751 1105 82-83 -0.03647823162128816 1105 84-85 0.001443226323424085 1105 86-87 -0.08851555262984334 1105 88-89 -0.07201495287315396 1105 90-91 0.09051250875940298 1105 92-93 0.020781319338929904 1105 94-95 0.10538401069402425 1105 96-97 0.04749875604007059 1105 98-99 0.06450655348984924 1105 100-101 -0.0011608833804857 1106 1 0.002825972856271619 1106 2 0.04220377321745872 1106 3 0.016192881493616085 1106 4 -0.00819886100769196 1106 5 0.07943952212175276 1106 6 -0.37266249306778576 1106 7 -0.2054036311641596 1106 8 -0.08602190207666638 1106 9 0.023324268509973933 1106 10-11 -0.0014139047098211677 1106 12-13 0.02456397983355174 1106 14-15 -0.03999353440526221 1106 16-17 0.010796594165562112 1106 18-19 -0.038656691544261435 1106 20-21 -0.03779758101833153 1106 22-23 -0.049888037863880186 1106 24-25 -0.027970279840907608 1106 26-27 -0.05585770239625987 1106 28-29 -0.0746039998185708 1106 30-31 -0.07411364579261459 1106 32-33 -0.3813795560009865 1106 34-35 -0.04978385041494704 1106 36-37 -0.022768866406373434 1106 38-39 0.0024295012999786536 1106 40-41 -0.028960196482636036 1106 42-43 -0.016673079817898895 1106 44-45 0.042840783391859816 1106 46-47 -0.7686187216045894 1106 48-49 -0.21888503052990416 1106 50-51 -0.15522847054297984 1106 52-53 -0.5497838660522163 1106 54-55 -0.1373350664597055 1106 56-57 -0.04684968734223105 1106 58-59 -0.13017769516930144 1106 60-61 -0.06468722736356369 1106 62-63 -0.08403541045412055 1106 64-65 -0.11984035898488798 1106 66-67 -0.17607733513964519 1106 68-69 -0.46082583587709536 1106 70-71 -0.15326441682855574 1106 72-73 0.03503636493643114 1106 74-75 -0.07825077584080731 1106 76-77 -0.18510658035746275 1106 78-79 -0.023598005390404353 1106 80-81 0.14041328205028947 1106 82-83 0.09322147834950556 1106 84-85 -0.01940573563646808 1106 86-87 -0.001066805681766425 1106 88-89 -0.4858200333230016 1106 90-91 -0.015196732258715429 1106 92-93 -0.0014981976436629907 1106 94-95 -0.12025880032823011 1106 96-97 -0.1306390164082103 1106 98-99 -0.0070077287959975365 1106 100-101 0.016519633888613328 1107 1 -0.03595683753528078 1107 2 0.05252638882195271 1107 3 -0.06992750537116166 1107 4 0.0012025763013809865 1107 5 -0.045028674272856506 1107 6 0.019639045315017256 1107 7 -0.0014341354114364435 1107 8 0.06493167550438272 1107 9 0.01846098897977555 1107 10-11 0.07472968470957397 1107 12-13 0.07048667461120317 1107 14-15 0.12158181594729456 1107 16-17 0.14081443823204864 1107 18-19 0.053955970292243194 1107 20-21 0.06311396404000646 1107 22-23 0.08056518336265839 1107 24-25 0.0261858222503335 1107 26-27 -0.02355362023678964 1107 28-29 0.11076168312902013 1107 30-31 0.11684796952062726 1107 32-33 0.17380910868936184 1107 34-35 0.10168901885223391 1107 36-37 0.1924466810361949 1107 38-39 0.272899189341139 1107 40-41 0.09710877297924725 1107 42-43 0.3034950803768126 1107 44-45 0.23114911922758097 1107 46-47 -0.05485192270669614 1107 48-49 0.02102283658862092 1107 50-51 0.13982328176542325 1107 52-53 0.0771155938091681 1107 54-55 0.19808935665614769 1107 56-57 0.15197957331434964 1107 58-59 0.19542007353215496 1107 60-61 0.09167789640472535 1107 62-63 0.033923953842545984 1107 64-65 0.028218355595086564 1107 66-67 0.0762438610675602 1107 68-69 -0.04420827614161027 1107 70-71 0.09361964490544494 1107 72-73 0.006261208452777112 1107 74-75 -0.034147859754575904 1107 76-77 -0.23598550035116972 1107 78-79 0.12189247313311569 1107 80-81 0.13434170860400485 1107 82-83 0.08315248781491391 1107 84-85 0.18919335838769058 1107 86-87 0.07397460594871319 1107 88-89 0.104597304095428 1107 90-91 0.255984159613476 1107 92-93 0.21153557212047502 1107 94-95 0.061924527699297016 1107 96-97 0.1488550580220931 1107 98-99 0.14701531420812586 1107 100-101 0.21960219449334062 1108 1 0.018192831661629327 1108 2 -0.028073236894300635 1108 3 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24-25 -0.43145265577804537 2316 26-27 -0.518828523995225 2316 28-29 -0.5560733361353272 2316 30-31 -0.6771125012894359 2316 32-33 -0.9297894681700072 2316 34-35 -0.9784131471241793 2316 36-37 -0.7370095721404581 2316 38-39 -0.6198072951199123 2316 40-41 -0.4438425955670411 2316 42-43 -0.5551103829054753 2316 44-45 -0.4872699376451308 2316 46-47 -0.416111745279089 2316 48-49 -0.4139842422372766 2316 50-51 -0.4531979418853922 2316 52-53 -0.42939885004203404 2316 54-55 -0.4283282123002792 2316 56-57 -0.4267990205726875 2316 58-59 -0.47964337936358703 2316 60-61 -0.7530801648788312 2316 62-63 -0.9109453804912846 2316 64-65 -0.5521732165135376 2316 66-67 -0.5403122540599625 2316 68-69 -0.5889780419391002 2316 70-71 -0.601363127012057 2316 72-73 -0.5287986208537205 2316 74-75 -0.5835489253463564 2316 76-77 -0.6216059438079427 2316 78-79 -0.6854744762055773 2316 80-81 -0.5084750067245807 2316 82-83 -0.4855049082353773 2316 84-85 -0.4577311603243075 2316 86-87 -0.3711656958994709 2316 88-89 -0.5491599250452808 2316 90-91 -0.5264874489234472 2316 92-93 -0.5549040590430394 2316 94-95 -0.574510822498155 2316 96-97 -0.5298584395271746 2316 98-99 -0.8952965036632179 2316 100-101 -0.9083289444841327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 6.0 14 26.0 15 75.0 16 210.0 17 481.0 18 968.0 19 1574.0 20 2261.0 21 3324.0 22 4806.0 23 5412.0 24 5018.0 25 4807.0 26 4942.0 27 5379.0 28 6659.0 29 8214.0 30 9775.0 31 12309.0 32 16574.0 33 22523.0 34 34572.0 35 60735.0 36 129811.0 37 289161.0 38 390400.0 39 108303.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.199259204070124 19.360832898718865 13.73789731745305 23.702010579757964 2 15.208478824209209 21.36659801269803 39.89067357696629 23.534249586126464 3 17.672337393474468 26.52866795229925 31.153843426967086 24.645151227259195 4 12.44842108248373 17.95486933340896 39.13457311798987 30.462136466117435 5 13.156469759342057 38.405834991337024 35.306483274472356 13.131211974848563 6 29.804460956000174 38.29743487940105 18.701877401698734 13.196226762900046 7 26.389617571572394 33.13227599506544 23.35807112573203 17.120035307630136 8 25.525626391390045 34.940905803780325 21.4218233767707 18.11164442805893 9 26.448020503949067 15.508681564879542 20.534346950640217 37.508950980531175 10-11 23.89140776767863 26.133318208244116 30.065767551011724 19.909506473065523 12-13 25.04302674304837 24.094355742098323 29.255792436510074 21.606825078343235 14-15 22.416091204287962 25.391538930561662 26.780181642868286 25.412188222282094 16-17 21.94448087857862 28.51197481672646 27.275676020589028 22.267868284105894 18-19 22.035947249007375 27.62483160805445 28.949943278502555 21.38927786443562 20-21 22.999374292090376 26.844641866417568 28.635797228096234 21.520186613395822 22-23 22.798191724685562 26.71579661954256 28.298968067155393 22.18704358861648 24-25 22.568124937963503 26.988358407895298 28.28811167987749 22.155404974263714 26-27 22.404023618819725 27.127521685665855 28.308728549746863 22.159726145767557 28-29 22.44507111155226 27.062226153169888 28.465447442678276 22.027255292599577 30-31 22.749581696751413 26.846959502573633 28.231924336741205 22.17153446393375 32-33 22.56493448945734 26.94475561164443 28.193151525034388 22.297158373863844 34-35 22.76517944500376 27.003291479375523 28.16434886490932 22.0671802107114 36-37 22.828738487035245 26.916176089369632 28.10818475691221 22.146900666682914 38-39 22.701936221454634 27.011829141030212 28.058261676817853 22.227972960697297 40-41 22.737573203068504 26.943559193454618 28.13022879060732 22.18863881286956 42-43 22.782283793939566 26.920517065354566 27.94739836649037 22.349800774215502 44-45 22.852783843568762 27.02110481686826 27.747419281652796 22.378692057910186 46-47 22.85928415073618 27.01185518143981 27.665266299489478 22.463594368334537 48-49 22.86588635253809 27.064675911442293 27.662673516793117 22.4067642192265 50-51 22.930816896615287 27.119875785204826 27.431919373821305 22.51738794435858 52-53 22.940122371424927 27.29109652170214 27.27509996738653 22.49368113948641 54-55 22.987092863321184 27.224362973781602 27.367977468343664 22.42056669455355 56-57 22.934849269032796 27.25666626490648 27.20118791032713 22.60729655573359 58-59 22.898336358351177 27.263756150475732 27.369085262963853 22.468822228209238 60-61 23.038060728954843 27.281722776304868 27.372298191637906 22.307918303102387 62-63 22.86610576433062 27.372483051951562 27.318286521061314 22.44312466265651 64-65 22.78964711637998 27.335274890455054 27.236188119513887 22.63888987365107 66-67 22.829488950306846 27.35775605549595 27.210414996616695 22.602339997580504 68-69 22.839111463247868 27.113097493285547 27.416106445742212 22.631684597724373 70-71 22.870978305325984 27.103786054949442 27.351401076513838 22.673834563210736 72-73 22.85200000531757 27.183605570865733 27.17833231191637 22.786062111900325 74-75 22.81652930356264 27.362207428552942 27.090789929059223 22.7304733388252 76-77 22.826723718483315 27.220292626897603 27.158032527689123 22.794951126929952 78-79 22.864039036909944 27.283829388993663 27.07981793173858 22.772313642357812 80-81 22.881542191909023 27.1703025963161 27.073348411156644 22.874806800618238 82-83 22.92718333026105 27.24674042510954 27.056864427208144 22.76921181742127 84-85 23.073988273329267 27.086154516966097 27.08566708733321 22.754190122371426 86-87 22.826267575770064 27.2063681549712 27.168126966064598 22.799237303194136 88-89 22.915172973044832 27.07636337854665 27.129405196059754 22.879058452348776 90-91 22.990763618832222 27.15825744174752 27.102247147197183 22.748731792223076 92-93 23.096631595366052 27.12080633268579 27.055136267600645 22.72742580434752 94-95 22.87413213717052 27.121414159408904 27.207740669491333 22.796713033929244 96-97 22.892930320604627 27.117482948825206 27.19157225302384 22.79801447754633 98-99 22.89399380344001 27.209031096238107 26.92477099669611 22.972204103625767 100-101 22.963784864512288 27.14380414900103 27.04427988032273 22.848131106163947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 63.0 1 46.5 2 60.5 3 84.0 4 59.5 5 62.5 6 103.5 7 187.5 8 383.5 9 496.0 10 405.5 11 346.0 12 388.5 13 445.5 14 480.0 15 560.5 16 635.5 17 674.5 18 742.0 19 870.0 20 980.5 21 1120.0 22 1335.0 23 1683.5 24 2161.5 25 2874.5 26 4087.0 27 5984.5 28 8208.5 29 11064.0 30 14121.0 31 17189.5 32 20774.0 33 23837.5 34 26233.0 35 28315.5 36 31546.0 37 33329.0 38 33465.0 39 34131.5 40 35013.5 41 36416.5 42 36754.5 43 37093.5 44 38233.5 45 39517.5 46 40260.0 47 40683.5 48 41454.0 49 42050.5 50 41958.5 51 41181.0 52 40320.5 53 39188.0 54 37610.0 55 35318.5 56 32260.0 57 29271.0 58 27124.0 59 24332.5 60 20129.5 61 16304.0 62 12930.5 63 9611.0 64 6699.5 65 4766.0 66 3613.5 67 2639.0 68 1844.5 69 1186.0 70 850.0 71 689.5 72 499.0 73 341.0 74 264.5 75 201.0 76 116.0 77 65.0 78 33.5 79 19.0 80 8.0 81 5.0 82 4.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08259716688172652 2 3.544942784623456E-4 3 0.0 4 0.0 5 2.658707088467592E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004253931341548147 20-21 0.0040766842023169745 22-23 0.0 24-25 0.0 26-27 0.0027030188732753853 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001816783177119521 38-39 9.305474809636572E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.018743884973696525 48-49 0.0016838478226961415 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.00234852459481304 62-63 0.005937779164244289 64-65 0.005671908455397529 66-67 0.0029688895821221445 68-69 0.0016395360378883485 70-71 3.1018249365455245E-4 72-73 0.0029688895821221445 74-75 0.002880266012506558 76-77 0.003279072075776697 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 3.1018249365455245E-4 88-89 0.0011521064050026232 90-91 3.544942784623456E-4 92-93 0.0 94-95 0.008286303759057327 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1128368.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.071423140240746 #Duplication Level Percentage of deduplicated Percentage of total 1 66.57845722557278 35.33413475442792 2 16.56977281027865 17.587628483039087 3 6.955011584125982 11.073370881792783 4 3.5993301489979026 7.640862934355738 5 2.034653772205782 5.399098564431004 6 1.246529809251573 3.9693066578228295 7 0.7779388884507212 2.8900426748351737 8 0.5305230783659778 2.252449182209913 9 0.3854692430519876 1.8411661175004306 >10 1.2946502260230137 10.266750010322703 >50 0.020628643922178152 0.7326543553093573 >100 0.006364612342785308 0.5256015346697639 >500 1.6748935267824937E-4 0.04497782543581028 >1k 5.024680580347482E-4 0.44195602384752714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.1962125831289083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1741 0.15429363470073593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.862356961558641E-5 0.0 18-19 0.0 0.0 0.0 8.862356961558641E-5 0.0 20-21 0.0 0.0 0.0 2.21558924038966E-4 0.0 22-23 0.0 0.0 0.0 3.5449427846234564E-4 0.0 24-25 0.0 0.0 0.0 6.203649873091048E-4 0.0 26-27 0.0 0.0 0.0 7.533003417324845E-4 0.0 28-29 0.0 0.0 0.0 0.0011964181898104164 0.0 30-31 0.0 0.0 0.0 0.0020826538859662807 0.0 32-33 0.0 0.0 0.0 0.004874296328857252 0.0 34-35 0.0 0.0 0.0 0.008064744835018362 0.0 36-37 0.0 0.0 0.0 0.01160968761964182 0.0 38-39 0.0 0.0 0.0 0.01772471392311728 0.0 40-41 0.0 0.0 0.0 0.02392836379620833 0.0 42-43 0.0 0.0 0.0 0.031239808289494207 0.0 44-45 0.0 0.0 0.0 0.03886143527643464 0.0 46-47 0.0 0.0 0.0 0.048565716149341354 0.0 48-49 0.0 0.0 0.0 0.05884605022474937 0.0 50-51 0.0 0.0 0.0 0.0691263843001574 0.0 52-53 0.0 0.0 0.0 0.07900791231229529 0.0 54-55 0.0 0.0 0.0 0.08955411709655006 0.0 56-57 0.0 0.0 0.0 0.1016955461338854 0.0 58-59 0.0 0.0 0.0 0.1160082526268026 0.0 60-61 0.0 0.0 0.0 0.13271379549934065 0.0 62-63 0.0 0.0 0.0 0.14587439558725523 0.0 64-65 0.0 0.0 0.0 0.15899068389036203 0.0 66-67 0.0 0.0 0.0 0.17543035605405327 0.0 68-69 0.0 0.0 0.0 0.19528203564794466 0.0 70-71 0.0 0.0 0.0 0.2143804149001035 0.0 72-73 0.0 0.0 0.0 0.23494108305091957 0.0 74-75 0.0 0.0 0.0 0.2565209222523148 0.0 76-77 0.0 0.0 0.0 0.27920855607390493 0.0 78-79 0.0 0.0 0.0 0.3049980148320406 0.0 80-81 0.0 0.0 0.0 0.3329144392609503 0.0 82-83 0.0 0.0 0.0 0.36300214114544194 0.0 84-85 0.0 0.0 0.0 0.39109581271358285 0.0 86-87 0.0 0.0 0.0 0.42308892134480947 0.0 88-89 0.0 0.0 0.0 0.45530358890007516 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1340 0.0 57.46553 1 GTATCAA 2595 0.0 43.41184 1 TCAACGC 2980 0.0 37.45435 4 CAACGCA 3045 0.0 36.654835 5 ATCAACG 3050 0.0 36.59474 3 AACGCAG 3180 0.0 35.24809 6 TATCAAC 3210 0.0 34.918667 2 ACGCAGA 3670 0.0 30.28311 7 CGCAGAG 3750 0.0 29.890377 8 GCAGAGT 4365 0.0 25.35259 9 TACATGG 3540 0.0 23.747652 2 GTACATG 3820 0.0 22.771147 1 GAGTACT 2370 0.0 22.647448 12-13 ACATGGG 3565 0.0 21.582718 3 CAGAGTA 4155 0.0 21.432898 10-11 GTACTTT 2520 0.0 20.925186 14-15 AGAGTAC 3890 0.0 20.878397 10-11 GAGTACA 2625 0.0 20.099758 1 CATGGGG 2360 0.0 19.320124 4 GTATAAC 300 3.6834535E-9 19.013287 1 >>END_MODULE