FastQCFastQC Report
Fri 10 Feb 2017
SRR2031476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147179
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA34142.319624402937919No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT27871.893612539832449No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14410.9790798959090632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.8907520774023467No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.7582603496422724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.7548631258535524No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT10140.6889569843523872No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC9220.6264480666399419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.44028020301809356No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5050.3431196026607057No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG4490.30507069622704325No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3660.2486767813342936No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC2770.18820619789508014No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2630.17869397128666453No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC2460.167143410405017No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2440.16578452088952908No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2420.16442563137404112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.15627229428111347No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA2280.1549134047656255No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC2180.14811895718818582No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG2180.14811895718818582No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2060.13996562009525815No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA2000.13588895154879432No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG1980.1345300620333064No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA1980.1345300620333064No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC1930.13113283824458652No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT1920.13045339348684257No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1920.13045339348684257No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC1910.1297739487290986No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC1890.12841505921361063No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1870.1270561696981227No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1870.1270561696981227No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA1860.12637672494037872No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC1840.12501783542489078No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT1830.1243383906671468No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT1830.1243383906671468No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT1770.12026172212068298No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA1770.12026172212068298No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA1720.11686449833196312No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG1690.1148261640587312No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC1670.11346727454324326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.11074949551226737No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG1560.10599338220805958No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA1560.10599338220805958No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA1520.1032756031770837No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG1500.10191671366159573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.10123726890385178No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT1480.1005578241461078No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG1480.1005578241461078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.051.3574941
CATGGGG1750.035.271944
GTATCAA8100.035.1714481
CATATAG550.002577270834.531971
TCATTGC300.003914391631.7190658-59
TAACTTT1351.1945303E-831.632786
CTATAAC1601.884473E-929.6759133
ATCAACG9850.029.4047583
TCAACGC9900.029.256254
CAACGCA9950.029.1092365
AACGCAG10050.028.799996
ACTTTAT1652.6375346E-928.757078
TATAGGT1004.937291E-528.4888733
AACTTTA1503.32966E-828.4695017
TATCTCA856.3950015E-427.9302675
GAGTACT6300.027.49038912-13
ATAGGTG1056.883884E-527.1322614
ACTAGGG700.00838414427.132263
ATAACTT1754.9058144E-927.132265
GGATGAC700.00841202927.1138118
Produced by FastQC (version 0.11.5)