##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031476_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147179 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.846866740499664 34.0 33.0 34.0 31.0 34.0 2 32.92191141399248 34.0 33.0 34.0 31.0 34.0 3 33.07022741016042 34.0 34.0 34.0 31.0 34.0 4 36.062264317599656 37.0 37.0 37.0 35.0 37.0 5 36.05123692918147 37.0 37.0 37.0 35.0 37.0 6 36.14546912263298 37.0 37.0 37.0 35.0 37.0 7 36.192289660889124 37.0 37.0 37.0 35.0 37.0 8 36.26884949619171 37.0 37.0 37.0 35.0 37.0 9 38.214167782088474 39.0 39.0 39.0 37.0 39.0 10-11 38.310628554345385 39.0 39.0 39.0 37.5 39.0 12-13 38.38113453685648 39.0 39.0 39.0 38.0 39.0 14-15 39.98024854089238 41.0 40.0 41.0 38.5 41.0 16-17 39.960351001161854 41.0 40.0 41.0 38.0 41.0 18-19 39.97953172667296 41.0 40.5 41.0 38.5 41.0 20-21 39.9234741369353 41.0 40.0 41.0 38.0 41.0 22-23 39.83850617275563 41.0 40.0 41.0 38.0 41.0 24-25 39.7332262075432 41.0 40.0 41.0 38.0 41.0 26-27 39.63773024684228 41.0 40.0 41.0 37.0 41.0 28-29 39.55586055075792 41.0 40.0 41.0 37.0 41.0 30-31 39.3526386237167 41.0 40.0 41.0 36.0 41.0 32-33 39.12555119955972 41.0 39.5 41.0 35.0 41.0 34-35 39.050054695303 41.0 39.0 41.0 35.0 41.0 36-37 38.93730083775539 41.0 39.0 41.0 35.0 41.0 38-39 38.76566969472547 41.0 39.0 41.0 35.0 41.0 40-41 38.687971789453655 41.0 39.0 41.0 35.0 41.0 42-43 38.78405886709381 41.0 39.0 41.0 35.0 41.0 44-45 38.72403331997092 41.0 40.0 41.0 35.0 41.0 46-47 38.50491238559849 41.0 39.5 41.0 35.0 41.0 48-49 38.272688359072966 41.0 39.0 41.0 35.0 41.0 50-51 37.30622914953899 40.0 38.0 40.5 34.0 40.5 52-53 37.394274998471246 39.5 38.0 40.5 34.5 41.0 54-55 37.8618552918555 40.0 39.0 41.0 35.0 41.0 56-57 37.922628228211906 41.0 39.0 41.0 35.0 41.0 58-59 37.993888394404095 41.0 39.0 41.0 35.0 41.0 60-61 37.86617995773854 41.0 39.0 41.0 35.0 41.0 62-63 37.670700303711804 41.0 38.5 41.0 35.0 41.0 64-65 37.46763464896487 40.0 37.5 41.0 34.0 41.0 66-67 37.2467777332364 40.0 37.0 41.0 34.0 41.0 68-69 36.903338791539554 39.0 36.5 41.0 34.0 41.0 70-71 36.56621189164215 39.0 36.0 41.0 33.5 41.0 72-73 36.17851052120207 38.0 36.0 40.5 33.0 41.0 74-75 35.750100218101764 37.0 35.0 39.5 33.0 41.0 76-77 35.3865497115757 37.0 35.0 39.0 32.5 41.0 78-79 34.95623356592992 36.0 35.0 39.0 31.5 40.5 80-81 34.53160437290646 36.0 35.0 37.5 31.0 39.5 82-83 34.167140013181225 35.5 35.0 37.0 31.0 39.0 84-85 33.776248649603545 35.0 35.0 37.0 30.0 39.0 86-87 33.51408488982803 35.0 35.0 36.0 30.0 37.0 88-89 33.280675232200245 35.0 35.0 36.0 29.5 37.0 90-91 32.99750304051529 35.0 35.0 36.0 29.0 37.0 92-93 32.93179054077008 35.0 35.0 35.5 29.0 36.0 94-95 32.82485952479634 35.0 35.0 35.0 29.0 36.0 96-97 32.66948409759544 35.0 35.0 35.0 29.0 36.0 98-99 32.58147561812487 35.0 34.5 35.0 28.0 36.0 100-101 32.25793421615855 35.0 34.0 35.0 26.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23288818090965435 1101 2 0.32913330384803885 1101 3 0.060623617153204634 1101 4 0.08154988162712584 1101 5 -0.1053222258172255 1101 6 0.0666666191564147 1101 7 0.0032628126165263893 1101 8 0.2980436356358638 1101 9 0.2359584228836411 1101 10-11 -0.0740471719885818 1101 12-13 -0.08828934031517832 1101 14-15 0.04900838549651354 1101 16-17 0.11040039290474368 1101 18-19 0.11691952976456577 1101 20-21 -0.09987843829686938 1101 22-23 -0.07371198748231933 1101 24-25 -0.04984647182863 1101 26-27 -0.017885455671759587 1101 28-29 -0.19940527956123333 1101 30-31 0.007460771821193646 1101 32-33 -0.21040831818837802 1101 34-35 -0.10835681912895723 1101 36-37 -0.11309259592086818 1101 38-39 -0.23308445334320282 1101 40-41 -0.215297893113096 1101 42-43 -0.1229381894366739 1101 44-45 -0.15039245153318603 1101 46-47 -0.7368138391739691 1101 48-49 -0.3621528253394075 1101 50-51 -0.17402531285348033 1101 52-53 -0.13719787200417954 1101 54-55 -0.39806266836710336 1101 56-57 -0.028806653140073024 1101 58-59 0.2856798200963837 1101 60-61 0.001969309184424617 1101 62-63 -0.239116485661512 1101 64-65 -0.2517045669020419 1101 66-67 -0.4447278327493578 1101 68-69 -0.2614240836732904 1101 70-71 -0.07718532929248312 1101 72-73 -0.7836860402364678 1101 74-75 -0.7973842167991165 1101 76-77 -0.5826628908226965 1101 78-79 -0.4924820795414888 1101 80-81 -0.37718230573995015 1101 82-83 -0.4767703039410378 1101 84-85 -0.5198996884854523 1101 86-87 -0.23369190391385786 1101 88-89 -0.11553978571707546 1101 90-91 -0.29670059171014174 1101 92-93 -0.3704640238469139 1101 94-95 -0.3195639879935044 1101 96-97 -0.8320865324086562 1101 98-99 -0.7058307123302683 1101 100-101 -0.8169971324003207 1102 1 0.3194473206946 1102 2 0.30493975546094276 1102 3 0.3060537246800905 1102 4 -0.18861140869545778 1102 5 0.1559680967634236 1102 6 0.36747307076932145 1102 7 0.23175743627244572 1102 8 0.30422643133479 1102 9 0.47950680998041406 1102 10-11 0.4057915376888417 1102 12-13 0.44611926183535644 1102 14-15 0.4140621489373686 1102 16-17 0.300588564947752 1102 18-19 0.42323673406564666 1102 20-21 0.5339925294450651 1102 22-23 0.5183579049908005 1102 24-25 0.37152449591330594 1102 26-27 0.4299640066938295 1102 28-29 0.5195463333419923 1102 30-31 0.6049070083803301 1102 32-33 0.5901293162202208 1102 34-35 0.47390124538716805 1102 36-37 0.5822031030038559 1102 38-39 0.4478295251514197 1102 40-41 0.3351053326933524 1102 42-43 0.4810940686278329 1102 44-45 0.4035053979291803 1102 46-47 0.10458401028839859 1102 48-49 -0.09535175007059138 1102 50-51 -0.06757369995025897 1102 52-53 0.25608169788829116 1102 54-55 0.6300287294823619 1102 56-57 0.4530481855696067 1102 58-59 0.153690572784555 1102 60-61 0.22643167477582438 1102 62-63 0.21773835304816913 1102 64-65 0.20447822879688005 1102 66-67 0.40796033929365905 1102 68-69 0.22876408836972217 1102 70-71 0.300771659954826 1102 72-73 0.19091073395708946 1102 74-75 0.23688997674926782 1102 76-77 0.23897689412353884 1102 78-79 0.48466845809292636 1102 80-81 0.239484360926717 1102 82-83 0.4851920616503591 1102 84-85 0.5450196663532552 1102 86-87 0.10421132189258486 1102 88-89 0.4710193540678773 1102 90-91 0.7804499459242678 1102 92-93 0.3855843632498619 1102 94-95 0.4673983775978954 1102 96-97 0.45232206974188216 1102 98-99 0.36312090057295876 1102 100-101 0.38246523319107695 1103 1 0.25215901424298437 1103 2 0.25882080384803885 1103 3 0.3720819504865389 1103 4 0.37842488162712584 1103 5 0.3228027741827759 1103 6 0.40729161915641754 1103 7 0.2975336459498621 1103 8 0.35689780230253376 1103 9 0.39533342288363826 1103 10-11 0.2853278280114182 1103 12-13 0.12681482635148456 1103 14-15 0.4281750521631764 1103 16-17 0.2874837262380723 1103 18-19 0.1400966130979029 1103 20-21 0.4759028117031292 1103 22-23 0.14373592918435207 1103 24-25 -0.006877721828630001 1103 26-27 0.07638537766157327 1103 28-29 0.07246972043876809 1103 30-31 0.07126285515452224 1103 32-33 0.32110209847828486 1103 34-35 0.26976818087103993 1103 36-37 -0.20345717925420814 1103 38-39 0.14478012999013146 1103 40-41 0.097202106886904 1103 42-43 0.2661243105633204 1103 44-45 0.27825338180014825 1103 46-47 0.45589449415936656 1103 48-49 0.6352430079939211 1103 50-51 0.9150371871465168 1103 52-53 0.7693125446624833 1103 54-55 0.9493331649662338 1103 56-57 1.072755846859927 1103 58-59 0.7565131534297151 1103 60-61 0.7337401425177603 1103 62-63 0.8569772643384894 1103 64-65 0.7141808497646238 1103 66-67 0.44173050058397934 1103 68-69 0.5789404996600425 1103 70-71 0.8426063373741854 1103 72-73 0.6749077097635379 1103 74-75 0.9252720332008835 1103 76-77 1.274889192510635 1103 78-79 0.4866845871251826 1103 80-81 0.739484360926717 1103 82-83 0.738594279392295 1103 84-85 0.7660378115145505 1103 86-87 0.7999018460861365 1103 88-89 0.5756060476162617 1103 90-91 0.536372324956524 1103 92-93 0.3248484761530861 1103 94-95 0.3965818453398313 1103 96-97 0.5002051342580103 1103 98-99 0.26370053766973456 1103 100-101 0.6827424509330129 1104 1 0.059838535519581626 1104 2 0.2280694740608027 1104 3 0.18846049658583297 1104 4 0.28279101637890136 1104 5 0.0443231642536972 1104 6 0.22499995248975324 1104 7 0.24085146509879962 1104 8 0.33545498315359623 1104 9 0.25404352926661744 1104 10-11 0.16886062942985802 1104 12-13 0.082100730606804 1104 14-15 0.2991856904610515 1104 16-17 0.10197840708913475 1104 18-19 0.2359798134525093 1104 20-21 0.3338095049655365 1104 22-23 0.09517099124109052 1104 24-25 0.060525868596904786 1104 26-27 0.21651170744880943 1104 28-29 0.3779883374600459 1104 30-31 0.32350687111196663 1104 32-33 0.515832816563389 1104 34-35 0.3317141028568571 1104 36-37 0.31075492180961817 1104 38-39 0.5339368232525388 1104 40-41 0.41812409270250583 1104 42-43 0.11713273254913759 1104 44-45 0.38746215839589127 1104 46-47 0.43251240196078555 1104 48-49 0.6054890186322197 1104 50-51 0.6224285878557367 1104 52-53 0.6502134755135529 1104 54-55 0.569401870639993 1104 56-57 0.6258919284202094 1104 58-59 0.48302024562829615 1104 60-61 0.7094161176950635 1104 62-63 0.8657594008633112 1104 64-65 0.7419124543745497 1104 66-67 0.8201657842719214 1104 68-69 0.7863596042699683 1104 70-71 0.722814670707514 1104 72-73 0.6052323994798527 1104 74-75 0.6622789037682537 1104 76-77 0.6984541304538965 1104 78-79 0.9392555091109998 1104 80-81 0.7483496091536708 1104 82-83 0.4362616109525774 1104 84-85 0.533380453358518 1104 86-87 0.5374073868662776 1104 88-89 0.40910560435384724 1104 90-91 0.5763490536799267 1104 92-93 0.5918586711885467 1104 94-95 0.339567217680262 1104 96-97 0.7744737512792881 1104 98-99 0.9033004933434938 1104 100-101 0.436371661925925 1105 1 0.07402293268270199 1105 2 -0.147817050761887 1105 3 -0.13778063816594255 1105 4 0.04874846318740822 1105 5 0.07269195857993793 1105 6 0.253368746815994 1105 7 -0.04992867674517498 1105 8 0.03758264272806855 1105 9 -0.24241037144260247 1105 10-11 -0.20702589539283878 1105 12-13 -0.31151629066979325 1105 14-15 -0.254005798900657 1105 16-17 -0.18880173475483986 1105 18-19 0.01612165742414362 1105 20-21 -0.152006097871336 1105 22-23 -0.29844603003550674 1105 24-25 -0.06358760658039841 1105 26-27 0.12076702659773986 1105 28-29 -0.09364286821371337 1105 30-31 0.27031538175026526 1105 32-33 0.0903009016697709 1105 34-35 0.019657365268201943 1105 36-37 -0.08286209946697198 1105 38-39 -0.24265892142830836 1105 40-41 -0.18471278673011682 1105 42-43 -0.134640317096256 1105 44-45 -0.4104101820296435 1105 46-47 -0.0852180944931149 1105 48-49 0.20832589806484236 1105 50-51 0.27845695665006076 1105 52-53 0.24241205707383529 1105 54-55 0.34954371461162737 1105 56-57 0.5585160418953876 1105 58-59 0.6993323023658817 1105 60-61 0.7094161176950635 1105 62-63 0.4224969895157926 1105 64-65 0.46531670969370253 1105 66-67 0.39463386937830336 1105 68-69 0.3785581858302578 1105 70-71 0.18026147921815294 1105 72-73 0.2967217611819777 1105 74-75 0.2899384782363441 1105 76-77 0.20554632903545667 1105 78-79 -0.09620548379680116 1105 80-81 0.067498545323879 1105 82-83 0.0036374974774062707 1105 84-85 -0.026903234584736424 1105 86-87 0.09414497551875911 1105 88-89 0.3381836185382454 1105 90-91 0.1366327416231954 1105 92-93 0.45710689813890326 1105 94-95 0.4920494871837988 1105 96-97 0.21419006333602653 1105 98-99 -0.004500925096223796 1105 100-101 0.24842839951457307 1106 1 0.38539959643476607 1106 2 0.4421470024781726 1106 3 0.46005284089749665 1106 4 0.45654988162712584 1106 5 0.5768695550046914 1106 6 0.1313926465536781 1106 7 0.3845413514293128 1106 8 0.4383404392888366 1106 9 0.2654104776781594 1106 10-11 0.2581446088333337 1106 12-13 -0.06785555036084645 1106 14-15 -0.06069481085052786 1106 16-17 0.04048030158053706 1106 18-19 0.0802186165225649 1106 20-21 0.08436813704559398 1106 22-23 0.39518070658161264 1106 24-25 0.1530644870754827 1106 26-27 0.23873554889444648 1106 28-29 0.23021800811000048 1106 30-31 -0.07273329210575241 1106 32-33 -0.11291973371349684 1106 34-35 0.23273907128199767 1106 36-37 0.22360831732112985 1106 38-39 -0.05454564055781219 1106 40-41 0.4910377233252561 1106 42-43 -0.1291025729983204 1106 44-45 -0.02065500861080949 1106 46-47 -0.41889146474474614 1106 48-49 0.46005037100761825 1106 50-51 0.49583770084514356 1106 52-53 0.23940030151179315 1106 54-55 0.8477706649662338 1106 56-57 0.2973919769969129 1106 58-59 0.4508624684982081 1106 60-61 0.12297387539447158 1106 62-63 0.07235611707821477 1106 64-65 0.34030456551804633 1106 66-67 0.629016459488092 1106 68-69 0.7066695236326481 1106 70-71 0.5127690086070587 1106 72-73 0.7627180693525801 1106 74-75 0.182924002378968 1106 76-77 0.2717320863462547 1106 78-79 0.4053489706868234 1106 80-81 0.6915391554472663 1106 82-83 0.9022822074744852 1106 84-85 0.7562989416515364 1106 86-87 0.1556916577299745 1106 88-89 0.20991683528749405 1106 90-91 0.25763730783324235 1106 92-93 0.5910085788928114 1106 94-95 0.4033241398603806 1106 96-97 0.010265065764855308 1106 98-99 0.07550490410808663 1106 100-101 -0.005239141532740632 1107 1 -0.206327505364861 1107 2 -0.1234810752369313 1107 3 -0.3228731148729409 1107 4 0.06112504502581828 1107 5 -0.2210085003270308 1107 6 -0.3109477599285526 1107 7 0.18953732242044907 1107 8 -0.18839427286086163 1107 9 -0.442799747051005 1107 10-11 -0.346922988982044 1107 12-13 -0.39662267364851544 1107 14-15 -0.5354687386864967 1107 16-17 -0.5460538554612739 1107 18-19 -0.45015563363412525 1107 20-21 -0.19170850365635062 1107 22-23 -0.3674211378091101 1107 24-25 -0.14584320385477412 1107 26-27 -0.275074998155425 1107 28-29 -0.2003039723716924 1107 30-31 -0.4087973981134496 1107 32-33 -0.14700962537792606 1107 34-35 -0.10084047925968065 1107 36-37 -0.1365403083391783 1107 38-39 -0.1419079827549652 1107 40-41 -0.38482403690394307 1107 42-43 -0.40365714368504513 1107 44-45 -0.20014735349396773 1107 46-47 -0.6134478261020746 1107 48-49 -0.7759600148818961 1107 50-51 -0.9263128945528294 1107 52-53 -0.7037011399780368 1107 54-55 -0.20941887751742172 1107 56-57 -0.1754569799374579 1107 58-59 -0.24552933023041845 1107 60-61 -0.08136402414891108 1107 62-63 -0.26044001507327863 1107 64-65 -0.14190064533341484 1107 66-67 -0.23525070856634756 1107 68-69 -0.192224737268063 1107 70-71 -0.32620493713562126 1107 72-73 0.27440219505765384 1107 74-75 0.013481796272778013 1107 76-77 0.06627501767403743 1107 78-79 0.124347985817991 1107 80-81 0.16595494916200693 1107 82-83 -0.06443370263384907 1107 84-85 -0.054785309400486426 1107 86-87 -0.10999909345634507 1107 88-89 -0.37910187722033584 1107 90-91 -0.026765951187265102 1107 92-93 -0.5179313441083551 1107 94-95 -0.1843515696928506 1107 96-97 -0.31035450626486494 1107 98-99 -0.20558561429105282 1107 100-101 -0.36323896246567955 1108 1 0.21080750150412797 1108 2 0.25110782614103044 1108 3 0.2629059950725292 1108 4 0.39604032748700035 1108 5 0.27493255125283866 1108 6 0.21013795673603397 1108 7 0.1869146172874423 1108 8 0.3129587099458462 1108 9 0.1999711617371389 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-0.5694286746059589 2316 90-91 -0.3144783694879223 2316 92-93 -0.4396306905135816 2316 94-95 -0.5276195435490578 2316 96-97 -0.42264208796421343 2316 98-99 -0.18610849010804031 2316 100-101 -0.24310824351142912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42.0 3 18.0 4 16.0 5 22.0 6 18.0 7 89.0 8 218.0 9 191.0 10 120.0 11 109.0 12 107.0 13 129.0 14 156.0 15 216.0 16 238.0 17 320.0 18 455.0 19 608.0 20 736.0 21 1110.0 22 1443.0 23 1068.0 24 762.0 25 531.0 26 603.0 27 533.0 28 570.0 29 599.0 30 721.0 31 857.0 32 1017.0 33 1402.0 34 2118.0 35 5741.0 36 7235.0 37 19004.0 38 66194.0 39 31557.0 40 306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.610799934743596 24.612540105497853 17.687204306922617 20.089455652835937 2 13.128641651312526 26.44629222955746 41.70162425297281 18.723441866157202 3 13.1721873661743 34.22403186666032 34.062251466559715 18.541529300605657 4 12.20062678876131 21.65820297894615 43.71001842296685 22.43115180932569 5 8.994960863385675 41.94724207577066 38.25935572496243 10.798441335881236 6 20.715116160483 44.94326255600655 21.20056295510637 13.141058328404077 7 21.796938227273035 35.52662898958779 29.418045552540484 13.258387230598684 8 20.85881224620282 37.17393226633654 25.72219539236959 16.245060095091045 9 23.933966820163654 19.283484222912996 25.274628104232843 31.507920852690507 10-11 19.75504626450652 30.126997940022708 33.62861940729762 16.489336388173147 12-13 21.356979475031185 30.059104025123457 31.467200494856012 17.116716004989346 14-15 19.285413096112517 32.08431948638429 29.61791670067194 19.012350716831254 16-17 17.376054054605213 32.996964866545895 31.739406772460153 17.887574306388736 18-19 17.011458319174675 31.84644764920961 34.092917046118714 17.049176985497002 20-21 17.0979032860978 32.33662962585381 33.96574574370476 16.599721344343628 22-23 18.798597865518847 30.701441233769543 33.90724288817943 16.592718012532174 24-25 18.146117695901776 31.658040080446742 33.312575078524056 16.883267145127427 26-27 16.29636183585894 32.439928264427934 34.154707902142874 17.109001997570246 28-29 17.603714854314436 30.305318841319252 35.31765066081543 16.773315643550884 30-31 18.332635848023056 31.339205819409145 33.30407297397529 17.024085358592508 32-33 16.532723355152307 32.91975995724707 33.58283891742489 16.964677770175744 34-35 17.66815716220539 31.546099870007012 34.44882291687935 16.336920050908248 36-37 18.53133645728053 30.765775422906906 32.822884444580566 17.880003675231997 38-39 16.31258063527834 33.30632725242629 33.386324256793785 16.99476785550158 40-41 16.75200822000769 31.197923223222883 35.77302435721649 16.277044199552932 42-43 17.3237636082986 31.378135603593066 32.91015292973664 18.387947858371696 44-45 16.5566358444384 32.46226543377754 33.367215121022575 17.61388360076149 46-47 18.12405230269197 31.160287488015666 33.79378106577274 16.921879143519618 48-49 17.596296447474046 31.653672939031523 32.88841721430807 17.86161339918636 50-51 16.456949249405543 32.229467933475526 32.187286328057226 19.126296489061698 52-53 17.58124721406527 31.813928482716147 33.3291367090072 17.27568759421138 54-55 18.26728273786216 31.35561767720276 32.06759531340976 18.30950427152532 56-57 17.078840603387246 32.893250013614335 31.60465337907749 18.42325600392093 58-59 17.233038609000943 31.228418728546302 33.12978189945807 18.40876076299469 60-61 18.63213811420983 31.94538086968366 31.577961657642934 17.844519358463582 62-63 16.82819353256872 32.88882229547498 32.87486038085379 17.408123791102515 64-65 18.13140963416337 31.118763219471322 33.009764953116644 17.740062193248658 66-67 17.982902261215074 31.746139741526825 31.59713835697058 18.673819640287526 68-69 17.021124604551485 32.52916964316086 31.95496139061809 18.494744361669557 70-71 18.423210257910654 31.049299344421797 33.540972786870746 16.9865176107968 72-73 18.364659607480196 31.226011922584863 31.9044832185535 18.50484525138144 74-75 17.101892082243143 32.84588086788768 31.354602955336514 18.69762409453266 76-77 17.78705480477415 31.813171110113093 33.240449652281605 17.15932443283115 78-79 17.9359423338064 31.82531336253399 31.97165698416426 18.267087319495353 80-81 17.09144916576396 33.21274551883418 31.365435580899437 18.330369734502423 82-83 18.701195164829173 31.910015126943676 31.95021736464111 17.43857234358604 84-85 17.802118481040242 31.424816394905026 31.574288136574268 19.198776987480464 86-87 16.420654783186066 33.366021417505145 32.3438377853823 17.869486013926487 88-89 18.76945078143621 31.097415642343968 32.734515986244 17.398617589975824 90-91 18.1284201355803 31.384933707222785 32.56445509242874 17.922191064768178 92-93 17.76903655208553 33.32528021450786 31.5626680096093 17.343015223797305 94-95 18.524919791918293 30.800243601215282 32.902154645944684 17.772681960921737 96-97 18.973505878510778 31.144880796864793 31.805192684519923 18.07642064010451 98-99 17.390830994301268 32.48277621842306 32.01054690822488 18.11584587905078 100-101 17.875385824788754 31.62146802290957 33.02047636387149 17.48266978843019 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2076.0 1 1178.5 2 294.5 3 256.0 4 167.0 5 165.0 6 264.5 7 487.0 8 763.5 9 857.0 10 579.5 11 294.0 12 261.5 13 261.0 14 245.0 15 209.5 16 191.5 17 197.5 18 202.5 19 196.5 20 216.0 21 262.0 22 305.0 23 371.0 24 415.0 25 499.5 26 815.5 27 1275.0 28 1498.0 29 1831.5 30 2818.0 31 3723.0 32 5210.0 33 9317.0 34 12698.0 35 11764.0 36 11257.5 37 11850.0 38 10365.0 39 8864.0 40 7431.5 41 5819.0 42 4714.0 43 4053.5 44 3815.0 45 3793.0 46 3140.5 47 2160.5 48 1889.0 49 1513.5 50 1133.0 51 819.0 52 497.0 53 366.5 54 303.0 55 260.0 56 245.0 57 232.0 58 195.5 59 179.0 60 181.5 61 190.0 62 163.5 63 121.5 64 101.0 65 78.0 66 51.0 67 40.5 68 37.0 69 35.5 70 29.0 71 21.0 72 18.5 73 18.0 74 15.0 75 13.5 76 11.5 77 7.0 78 5.0 79 4.0 80 3.0 81 4.0 82 3.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045522798768846094 2 0.06522669674342128 3 0.04484335401110213 4 0.05435558061951773 5 0.08832781850671631 6 0.06658558625890922 7 0.09512226608415603 8 0.11007005075452339 9 0.11007005075452339 10-11 0.061150028196957446 12-13 0.04450363163223014 14-15 0.09716060035738794 16-17 0.04654196590546206 18-19 0.02513945603652695 20-21 0.02989556934073475 22-23 0.07983475903491666 24-25 0.16952146705712093 26-27 0.17020091181486488 28-29 0.13690811868541028 30-31 0.1511764585980337 32-33 0.1960198126091358 34-35 0.16850229992050497 36-37 0.16952146705712093 38-39 0.20281426018657553 40-41 0.14981756908254573 42-43 0.08017448141378865 44-45 0.0672650310166532 46-47 0.07609781286732482 48-49 0.12569728018263476 50-51 0.13283145013894646 52-53 0.16000924044870532 54-55 0.2272742714653585 56-57 0.1868473083795922 58-59 0.13962589771638617 60-61 0.23372899666392624 62-63 0.23848510996813405 64-65 0.25716984080609323 66-67 0.13622867392766633 68-69 0.1318122830023305 70-71 0.14234367674736206 72-73 0.15695173903885745 74-75 0.15253534811352162 76-77 0.1501572914614177 78-79 0.1797131384232805 80-81 0.27041901358210074 82-83 0.28604624301021203 84-85 0.2231976029188947 86-87 0.22863316098084646 88-89 0.22693454908648653 90-91 0.17325841322471278 92-93 0.16170785234306526 94-95 0.14777923480931382 96-97 0.13792728582202624 98-99 0.14709979005156987 100-101 0.1722392460880968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 147179.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.867929527989727 #Duplication Level Percentage of deduplicated Percentage of total 1 64.25353425508777 14.050917590145334 2 10.905701413702035 4.769702199362681 3 5.170110299829113 3.3917882306579066 4 3.6290197296877427 3.174365908179835 5 2.370669566568277 2.592081750793252 6 1.7585831909274505 2.3073943972985274 7 1.5193413080627622 2.325739405757615 8 1.1309616280876185 1.9785431345504454 9 0.832686033866708 1.6388207556784595 >10 7.289109833773496 32.23829486543597 >50 0.8295789964269069 12.251068426881552 >100 0.2796333695821035 9.586286087009697 >500 0.00932111231940345 1.4098478723187413 >1k 0.02174926207860805 8.28514937592999 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 3414 2.319624402937919 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 2787 1.893612539832449 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1441 0.9790798959090632 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.8907520774023467 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.7582603496422724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1111 0.7548631258535524 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 1014 0.6889569843523872 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 922 0.6264480666399419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.44028020301809356 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 505 0.3431196026607057 No Hit AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG 449 0.30507069622704325 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 366 0.2486767813342936 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 277 0.18820619789508014 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 263 0.17869397128666453 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 246 0.167143410405017 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 244 0.16578452088952908 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 242 0.16442563137404112 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 230 0.15627229428111347 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 228 0.1549134047656255 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 218 0.14811895718818582 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 218 0.14811895718818582 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 206 0.13996562009525815 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 200 0.13588895154879432 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 198 0.1345300620333064 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 198 0.1345300620333064 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 193 0.13113283824458652 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 192 0.13045339348684257 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 192 0.13045339348684257 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 191 0.1297739487290986 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 189 0.12841505921361063 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 187 0.1270561696981227 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 187 0.1270561696981227 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 186 0.12637672494037872 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 184 0.12501783542489078 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 183 0.1243383906671468 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 183 0.1243383906671468 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 177 0.12026172212068298 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 177 0.12026172212068298 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 172 0.11686449833196312 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 169 0.1148261640587312 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 167 0.11346727454324326 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.11074949551226737 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 156 0.10599338220805958 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 156 0.10599338220805958 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 152 0.1032756031770837 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 150 0.10191671366159573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.10123726890385178 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 148 0.1005578241461078 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 148 0.1005578241461078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.794447577439716E-4 0.0 14-15 0.0 0.0 0.0 0.0010191671366159573 0.0 16-17 0.0 0.0 0.0 0.0027177790309758864 0.0 18-19 0.0 0.0 0.0 0.0037369461675918437 0.0 20-21 0.0 0.0 0.0 0.004076668546463829 0.0 22-23 0.0 0.0 0.0 0.004756113304207801 0.0 24-25 0.0 0.0 0.0 0.008832781850671632 0.0 26-27 0.0 0.0 0.0 0.013588895154879432 0.0 28-29 0.0 0.0 0.0 0.017665563701343263 0.0 30-31 0.0 0.0 0.0 0.024799733657654963 0.0 32-33 0.0 0.0 0.0 0.03770918405479043 0.0 34-35 0.0 0.0 0.0 0.05809252678710958 0.0 36-37 0.0 0.0 0.0 0.08527031709686844 0.0 38-39 0.0 0.0 0.0 0.1104097731333954 0.0 40-41 0.0 0.0 0.0 0.13996562009525815 0.0 42-43 0.0 0.0 0.0 0.1623872971008092 0.0 44-45 0.0 0.0 0.0 0.18107202793876845 0.0 46-47 0.0 0.0 0.0 0.21572371058371098 0.0 48-49 0.0 0.0 0.0 0.23984399948362198 0.0 50-51 0.0 0.0 0.0 0.25241372750188545 0.0 52-53 0.0 0.0 0.0 0.26939984644548476 0.0 54-55 0.0 0.0 0.0 0.2948790248608837 0.0 56-57 0.0 0.0 0.0 0.3217170927917705 0.0 58-59 0.0 0.0 0.0 0.34991405023814537 0.0 60-61 0.0 0.0 0.0 0.37573295103241633 0.0 62-63 0.0 0.0 0.0 0.3998532399323273 0.0 64-65 0.0 0.0 0.0 0.4256721407265982 0.0 66-67 0.0 0.0 0.0 0.4576060443405649 0.0 68-69 0.0 0.0 0.0 0.4922577269855074 0.0 70-71 0.0 0.0 0.0 0.5275888543881939 0.0 72-73 0.0 0.0 0.0 0.5608816475176486 0.0 74-75 0.0 0.0 0.0 0.5945141630259752 0.0 76-77 0.0 0.0 0.0 0.6366397380061015 0.0 78-79 0.0 0.0 0.0 0.6882775395946432 0.0 80-81 0.0 0.0 0.0 0.744331732108521 0.0 82-83 0.0 0.0 0.0 0.7942709218027029 0.0 84-85 0.0 0.0 0.0 0.8431909443602688 0.0 86-87 0.0 0.0 0.0 0.8989054144952744 0.0 88-89 0.0 0.0 0.0 0.9719457259527514 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 490 0.0 51.357494 1 CATGGGG 175 0.0 35.27194 4 GTATCAA 810 0.0 35.171448 1 CATATAG 55 0.0025772708 34.53197 1 TCATTGC 30 0.0039143916 31.71906 58-59 TAACTTT 135 1.1945303E-8 31.63278 6 CTATAAC 160 1.884473E-9 29.675913 3 ATCAACG 985 0.0 29.404758 3 TCAACGC 990 0.0 29.25625 4 CAACGCA 995 0.0 29.109236 5 AACGCAG 1005 0.0 28.79999 6 ACTTTAT 165 2.6375346E-9 28.75707 8 TATAGGT 100 4.937291E-5 28.488873 3 AACTTTA 150 3.32966E-8 28.469501 7 TATCTCA 85 6.3950015E-4 27.930267 5 GAGTACT 630 0.0 27.490389 12-13 ATAGGTG 105 6.883884E-5 27.132261 4 ACTAGGG 70 0.008384144 27.13226 3 ATAACTT 175 4.9058144E-9 27.13226 5 GGATGAC 70 0.008412029 27.113811 8 >>END_MODULE