##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	147179
Sequences flagged as poor quality	0
Sequence length	101
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.29592536978781	34.0	33.0	34.0	31.0	34.0
2	33.38703891180128	34.0	34.0	34.0	31.0	34.0
3	33.45883583935208	34.0	34.0	34.0	31.0	34.0
4	36.65090807791872	37.0	37.0	37.0	35.0	37.0
5	36.587006298452906	37.0	37.0	37.0	35.0	37.0
6	36.6552837021586	37.0	37.0	37.0	37.0	37.0
7	36.6637156116022	37.0	37.0	37.0	37.0	37.0
8	36.670673125921496	37.0	37.0	37.0	37.0	37.0
9	38.58958818853233	39.0	39.0	39.0	38.0	39.0
10-11	38.597860428457864	39.0	39.0	39.0	38.0	39.0
12-13	38.6001671434104	39.0	39.0	39.0	38.0	39.0
14-15	40.254071572710785	41.0	40.5	41.0	39.0	41.0
16-17	40.28915810000068	41.0	40.0	41.0	39.0	41.0
18-19	40.306844047044756	41.0	41.0	41.0	39.0	41.0
20-21	40.297291053750875	41.0	41.0	41.0	39.0	41.0
22-23	40.23070207026818	41.0	40.5	41.0	39.0	41.0
24-25	40.19327825301164	41.0	40.5	41.0	38.0	41.0
26-27	40.10155660793999	41.0	40.0	41.0	38.0	41.0
28-29	40.00873765958459	41.0	40.0	41.0	38.0	41.0
30-31	39.81217768839305	41.0	40.0	41.0	37.0	41.0
32-33	39.61043015647613	41.0	40.0	41.0	35.0	41.0
34-35	39.52790819342434	41.0	40.0	41.0	35.0	41.0
36-37	39.42319216736084	41.0	40.0	41.0	35.0	41.0
38-39	39.292704801636106	41.0	40.0	41.0	35.0	41.0
40-41	39.210906447251304	41.0	40.0	41.0	35.0	41.0
42-43	39.01503611248887	41.0	39.0	41.0	35.0	41.0
44-45	38.856582800535406	41.0	39.0	41.0	35.0	41.0
46-47	38.627028991907814	41.0	39.0	41.0	35.0	41.0
48-49	38.349102113752636	41.0	39.0	41.0	35.0	41.0
50-51	38.24201482548462	41.0	39.0	41.0	35.0	41.0
52-53	38.29759340666807	41.0	39.0	41.0	35.0	41.0
54-55	38.24819777278009	41.0	39.0	41.0	35.0	41.0
56-57	38.2156659577793	41.0	39.0	41.0	35.0	41.0
58-59	38.23287289626917	41.0	39.0	41.0	35.0	41.0
60-61	38.11149688474579	41.0	39.0	41.0	35.0	41.0
62-63	37.91881654312096	41.0	38.5	41.0	35.0	41.0
64-65	37.70207026817685	40.5	37.5	41.0	35.0	41.0
66-67	37.43396136677107	40.0	37.0	41.0	35.0	41.0
68-69	37.01898368653137	39.0	37.0	41.0	34.0	41.0
70-71	36.67746077905136	39.0	36.0	41.0	34.0	41.0
72-73	36.291101312007825	38.0	36.0	40.5	34.0	41.0
74-75	35.83993980119446	37.0	35.0	39.5	33.0	41.0
76-77	34.636276235060706	36.0	34.5	39.0	31.0	39.5
78-79	35.000346516826454	36.0	35.0	39.0	32.0	40.0
80-81	34.75780172443079	36.0	35.0	37.5	32.0	39.5
82-83	34.410075486312586	35.5	35.0	37.0	32.0	39.0
84-85	34.02680749291679	35.0	35.0	37.0	32.0	39.0
86-87	33.77519551022904	35.0	35.0	36.0	31.5	37.0
88-89	33.54817263332404	35.0	35.0	36.0	31.0	37.0
90-91	33.333440912086644	35.0	35.0	36.0	31.0	37.0
92-93	33.217891139360916	35.0	35.0	36.0	31.0	36.0
94-95	33.12453882687068	35.0	35.0	35.0	31.0	36.0
96-97	32.972635362381865	35.0	35.0	35.0	30.0	36.0
98-99	32.88860503196787	35.0	35.0	35.0	30.0	36.0
100-101	32.65235529525272	35.0	34.5	35.0	29.0	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.057902872913189185
1101	2	-0.19476257564989652
1101	3	-0.10556276233445061
1101	4	0.04870860164471935
1101	5	-0.18311506470650585
1101	6	-0.20185736033398172
1101	7	-0.14415025478211874
1101	8	-0.14196931018683046
1101	9	-0.10315870421757012
1101	10-11	-0.24931582422493648
1101	12-13	-0.05104197582910075
1101	14-15	0.0493182071895788
1101	16-17	0.014093494069634005
1101	18-19	0.006561882881278791
1101	20-21	-0.06180379458584895
1101	22-23	-0.14698283267552625
1101	24-25	-0.07591582621638082
1101	26-27	-0.06159759749569105
1101	28-29	0.011882342103938015
1101	30-31	-0.12834429573279493
1101	32-33	-0.12187108226966359
1101	34-35	-0.1800830699659599
1101	36-37	-0.13913354871176864
1101	38-39	0.07625036290758658
1101	40-41	0.03268572802664238
1101	42-43	0.1202607341372186
1101	44-45	0.22690760398771914
1101	46-47	0.2030891718045993
1101	48-49	-0.1444782075476141
1101	50-51	-3.733312193077154E-5
1101	52-53	0.07724263318355895
1101	54-55	0.2489554205324751
1101	56-57	0.008668295914269208
1101	58-59	-0.20679553133185635
1101	60-61	-0.010792870773187246
1101	62-63	-0.10572482733766009
1101	64-65	-0.0317014370574924
1101	66-67	0.0770036108143799
1101	68-69	0.2205096538574267
1101	70-71	-0.08582966805756342
1101	72-73	-0.10328441070973327
1101	74-75	-0.0712416642296958
1101	76-77	-0.31454817298173054
1101	78-79	-0.1066594630391009
1101	80-81	-0.3036902690809953
1101	82-83	0.04205474192153957
1101	84-85	-0.20900462667754738
1101	86-87	-0.21502282901621328
1101	88-89	-0.26642718228630713
1101	90-91	-0.20935384393109757
1101	92-93	-0.5503835599311131
1101	94-95	-0.360491180032362
1101	96-97	-0.3728551304901089
1101	98-99	-0.29974090101509887
1101	100-101	0.20368308317029005
1102	1	-0.13107562171046538
1102	2	-0.14476257564989936
1102	3	0.20492110863329316
1102	4	0.19521397798880002
1102	5	0.10156235464833685
1102	6	0.14599210203160595
1102	7	0.21794651941143428
1102	8	0.09915972207123502
1102	9	0.04227140330931434
1102	10-11	0.04221643383958451
1102	12-13	0.031753723095626185
1102	14-15	-0.1217839433480492
1102	16-17	-0.040207581199190656
1102	18-19	0.25011026997804464
1102	20-21	0.022739216166840492
1102	22-23	0.1885010382922232
1102	24-25	0.24948739959006616
1102	26-27	0.23033788637527408
1102	28-29	0.4016672883404979
1102	30-31	0.27622559674031777
1102	32-33	0.20890848762280712
1102	34-35	0.35244381175446904
1102	36-37	0.22981806419145556
1102	38-39	0.4028632661333944
1102	40-41	0.4844330398546006
1102	42-43	0.41058331478238586
1102	44-45	0.4140043781812679
1102	46-47	0.5556429352454515
1102	48-49	0.38522609352765613
1102	50-51	-0.004203999788593649
1102	52-53	0.04834478372119122
1102	54-55	-0.2955338267793479
1102	56-57	0.05786184430137098
1102	58-59	0.0948173718939529
1102	60-61	0.3159544410547781
1102	62-63	-0.015267838090345265
1102	64-65	0.014938347888744374
1102	66-67	0.12969178285739957
1102	68-69	0.26943438504022055
1102	70-71	0.38688538570587383
1102	72-73	0.05625860004295191
1102	74-75	0.15832822824341974
1102	76-77	0.31986042916881274
1102	78-79	0.3325878487888616
1102	80-81	0.456390376080293
1102	82-83	0.13667839783551727
1102	84-85	0.33387171740847776
1102	86-87	0.17825674087625742
1102	88-89	0.2216104521222917
1102	90-91	0.17451712381083695
1102	92-93	0.2187024615742672
1102	94-95	0.1319819382472076
1102	96-97	-0.02083900145785833
1102	98-99	0.2982429699526463
1102	100-101	0.2449465240305102
1103	1	0.1917570395798549
1103	2	0.14429992435010064
1103	3	0.2069372376655494
1103	4	0.15287526831138365
1103	5	0.157509935293497
1103	6	0.20647597299934972
1103	7	0.2683497452178827
1103	8	0.27834318981317097
1103	9	0.33486212911576274
1103	10-11	0.22959042577506494
1103	12-13	0.2812496908375621
1103	14-15	0.28421404052291876
1103	16-17	0.19013516073629688
1103	18-19	0.22062438288127595
1103	20-21	0.2777795387474882
1103	22-23	0.28426716732447943
1103	24-25	0.10924042378361776
1103	26-27	0.1915274025043061
1103	28-29	0.1733406754372666
1103	30-31	0.13988487093386937
1103	32-33	0.2494830843969993
1103	34-35	0.20715651336737295
1103	36-37	0.30227270128823136
1103	38-39	0.3184378629075866
1103	40-41	0.25560239469330526
1103	42-43	0.2999482341372186
1103	44-45	0.2144076039877163
1103	46-47	0.3549120884712593
1103	48-49	0.2841676257857202
1103	50-51	0.5231397502114064
1103	52-53	0.6931280498502232
1103	54-55	0.24088250386581223
1103	56-57	0.3227307959142749
1103	58-59	0.39320446866814507
1103	60-61	0.42696754589348274
1103	62-63	0.30391058932900705
1103	64-65	0.32819439627584046
1103	66-67	0.33924319414771986
1103	68-69	0.20592632052409243
1103	70-71	0.9120869986091051
1103	72-73	1.1667676726235996
1103	74-75	0.7094875024369713
1103	76-77	0.37555599368494086
1103	78-79	0.7951634536275662
1103	80-81	0.5088097309190047
1103	82-83	1.0378880752548767
1103	84-85	0.9326620399891183
1103	86-87	0.5391438376504496
1103	88-89	0.33148948438035575
1103	90-91	0.245333656068901
1103	92-93	0.26784560673555546
1103	94-95	0.3553942366343037
1103	96-97	0.43417611950988544
1103	98-99	0.5893215989849026
1103	100-101	0.5805059998369586
1104	1	0.22439223461531554
1104	2	0.2080743037827233
1104	3	0.1435507128428526
1104	4	0.14179370802769853
1104	5	0.20748777217293224
1104	6	0.23218519285750716
1104	7	0.17482137642355866
1104	8	0.17700232101884694
1104	9	0.14985548017958905
1104	10-11	0.16141112612967135
1104	12-13	0.2806956128233793
1104	14-15	0.12813026392717575
1104	16-17	0.16675306853771588
1104	18-19	0.1177320956472343
1104	20-21	0.18757563803826827
1104	22-23	0.11826539427483596
1104	24-25	0.2509458759112775
1104	26-27	0.2960265159794844
1104	28-29	0.19007383146563228
1104	30-31	0.21296776100479065
1104	32-33	0.15889132907784642
1104	34-35	-0.0254731408879465
1104	36-37	0.09446574206837255
1104	38-39	0.0700446891487232
1104	40-41	0.25139140178550434
1104	42-43	0.6725656986762232
1104	44-45	0.47105654015792453
1104	46-47	0.3858019377620394
1104	48-49	0.46695796266515543
1104	50-51	0.44792365978587156
1104	52-53	0.4863738388573182
1104	54-55	0.6419518744331896
1104	56-57	0.6382782249922911
1104	58-59	0.4526016317887098
1104	60-61	0.5137638668154736
1104	62-63	0.6124489315275881
1104	64-65	0.5657276409566876
1104	66-67	0.7768263058498448
1104	68-69	0.9233465332900508
1104	70-71	0.9105355801693875
1104	72-73	0.8132936034746621
1104	74-75	0.812268974068175
1104	76-77	0.784742607160112
1104	78-79	0.7085887639112514
1104	80-81	0.5850508656707731
1104	82-83	0.5223738908577147
1104	84-85	0.6972896995635907
1104	86-87	0.4819629865866162
1104	88-89	0.6969593425363811
1104	90-91	0.729653248267482
1104	92-93	0.7522760145369745
1104	94-95	0.7430549192584195
1104	96-97	0.48645338014818407
1104	98-99	0.7758796663607868
1104	100-101	0.5351547143759632
1105	1	-0.23660067318610345
1105	2	-0.1323512281321655
1105	3	0.02298333695632948
1105	4	-0.049695653674433515
1105	5	-0.08329236967104237
1105	6	-0.03022615466022671
1105	7	-0.16560415549133722
1105	8	-0.1421466151513684
1105	9	-0.1976622503168599
1105	10-11	0.012474955916907504
1105	12-13	0.06438355608578661
1105	14-15	4.706894590853494E-4
1105	16-17	0.1561147706653756
1105	18-19	0.17092358500893567
1105	20-21	-0.09965840451491914
1105	22-23	-0.20088354189537938
1105	24-25	-0.06465696096815776
1105	26-27	-0.04085291664463142
1105	28-29	-0.1929048919386176
1105	30-31	-0.1806492602718066
1105	32-33	-0.05742072765973916
1105	34-35	-0.16022491393758997
1105	36-37	-0.05447042814439129
1105	38-39	0.013307100496234625
1105	40-41	-0.12094902374641237
1105	42-43	0.09100541498828107
1105	44-45	-0.05022005558675602
1105	46-47	0.06310690230104399
1105	48-49	0.33575228890628495
1105	50-51	0.12168252503410315
1105	52-53	-0.06681765050438315
1105	54-55	0.014292299965099176
1105	56-57	-0.042572838837500626
1105	58-59	-0.03321397104816981
1105	60-61	-0.3656687572980033
1105	62-63	-0.38400496918163185
1105	64-65	-0.33498157890146274
1105	66-67	-0.06005312677426389
1105	68-69	-0.0837456652915094
1105	70-71	-0.3341452708944388
1105	72-73	-0.13351490716363656
1105	74-75	-0.1522700330240241
1105	76-77	-0.14788150631505914
1105	78-79	-0.4297091084291722
1105	80-81	-0.46814062369092113
1105	82-83	-0.2825906481493732
1105	84-85	-0.5261145557555622
1105	86-87	-0.2343490701509694
1105	88-89	-0.501622217747304
1105	90-91	-0.3448148368388999
1105	92-93	-0.2760927797892663
1105	94-95	-0.3491436623018629
1105	96-97	-0.556099811341177
1105	98-99	-0.4794394825753798
1105	100-101	-0.6137814558368007
1106	1	0.20641885464834786
1106	2	0.20797715037749498
1106	3	0.21121805958335926
1106	4	0.09379992584563013
1106	5	0.15215890789623643
1106	6	-0.16852402700065028
1106	7	0.015781252067199603
1106	8	0.0933046624159104
1106	9	0.21339380719795287
1106	10-11	0.17688280591205086
1106	12-13	0.17144660864578043
1106	14-15	0.13898715696127795
1106	16-17	0.17493824292807858
1106	18-19	0.041150923977163245
1106	20-21	0.12581036066529805
1106	22-23	-0.01410612034675296
1106	24-25	0.11089924227677272
1106	26-27	-0.05783047420801779
1106	28-29	0.066106086396168
1106	30-31	0.32428127504346094
1106	32-33	-0.17592359368519084
1106	34-35	0.1655790304906617
1106	36-37	-0.0988938226843743
1106	38-39	0.08481200674320633
1106	40-41	0.09507157277550249
1106	42-43	0.10142511769885942
1106	44-45	0.14163363138497687
1106	46-47	-0.2067852574191491
1106	48-49	0.16847841345695258
1106	50-51	0.5163439454168852
1106	52-53	-0.027494809738819015
1106	54-55	0.4673914935918404
1106	56-57	0.4549011726265988
1106	58-59	0.42916337277773664
1106	60-61	0.35125050822225035
1106	62-63	0.573441839329007
1106	64-65	0.6726935401114602
1106	66-67	0.47574790305183257
1106	68-69	0.05909412874326847
1106	70-71	0.46902193011595017
1106	72-73	0.5839872787879798
1106	74-75	0.7594660983273798
1106	76-77	0.6021184936849409
1106	78-79	0.294253778970031
1106	80-81	0.3512754843436596
1106	82-83	0.48052506155624286
1106	84-85	0.6629702591672029
1106	86-87	0.65815068696552
1106	88-89	0.17267099122967267
1106	90-91	0.3173584848360278
1106	92-93	0.18854338070816112
1106	94-95	0.1390521989630713
1106	96-97	0.2759462393728995
1106	98-99	0.22577279761504343
1106	100-101	0.3441511196999727
1107	1	0.052510716050441886
1107	2	0.1673289276180725
1107	3	-0.029582370177585915
1107	4	0.065865464389816
1107	5	0.03894375882291001
1107	6	0.043894273652945515
1107	7	-0.028627378965126127
1107	8	0.06505683360401804
1107	9	-0.013126024479007015
1107	10-11	0.05697502544826705
1107	12-13	0.1487946254780823
1107	14-15	0.179792063398736
1107	16-17	0.12406081433106664
1107	18-19	0.008359268502196926
1107	20-21	0.1186700616233054
1107	22-23	0.04713481438330547
1107	24-25	-0.06848118569349992
1107	26-27	0.023533121458555684
1107	28-29	0.10093462968563216
1107	30-31	0.042407338254129456
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2316	76-77	-0.32121483964839115
2316	78-79	-0.20777057415021005
2316	80-81	-0.23896804685877981
2316	82-83	-0.06850081363401017
2316	84-85	0.33516203998912175
2316	86-87	0.42442161542822987
2316	88-89	0.3299617066025746
2316	90-91	0.20397948940223642
2316	92-93	0.06461644006888889
2316	94-95	-0.10938006892124719
2316	96-97	-0.040355130490112856
2316	98-99	-0.5627964565706591
2316	100-101	-0.5310391390519271
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	9.0
15	49.0
16	91.0
17	219.0
18	352.0
19	526.0
20	679.0
21	1065.0
22	1667.0
23	1229.0
24	806.0
25	607.0
26	668.0
27	686.0
28	762.0
29	920.0
30	856.0
31	852.0
32	1027.0
33	1299.0
34	1829.0
35	4955.0
36	5899.0
37	16053.0
38	69034.0
39	34976.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.35573947756778	24.844433261018885	17.366348619111417	20.43347864230191
2	12.946140414053636	26.65869451484247	41.813709836321756	18.581455234782133
3	13.043980459168766	34.59732706432304	33.97767344526053	18.381019031247664
4	11.803314331528275	22.15261688148445	43.43282669402564	22.611242092961632
5	8.668356219297591	42.17993056074576	38.29214765693475	10.8595655630219
6	20.445851650031592	45.42224094469999	21.022020804598483	13.109886600669931
7	21.708939454677637	35.68172089768241	28.957256130290325	13.652083517349622
8	20.916027422390425	37.54543786817413	25.431617282356854	16.10691742707859
9	23.737761501301136	19.443670632359236	24.909124263651744	31.90944360268788
10-11	19.76810550418198	30.184673085154813	33.42086846628935	16.626352944373856
12-13	21.621630803307536	30.20505642788713	31.145747694983662	17.027565073821673
14-15	19.219453862303727	32.1530245483391	29.362205205905735	19.265316383451445
16-17	17.277261022292585	33.37126899897404	31.372342521691277	17.979127457042104
18-19	17.077918768791726	31.598294518337326	34.13375460768825	17.19003210518269
20-21	16.70817012226816	32.25684807017425	34.14709549418555	16.88788631337204
22-23	18.49278769389655	30.866835621929763	34.042220697246215	16.59815598692748
24-25	18.266532589567806	31.862561914403546	33.26901256293357	16.601892933095076
26-27	16.334753392174047	32.857715539792224	34.19420153964274	16.613329528390985
28-29	17.332635770048714	30.188070308943534	35.65556227450927	16.823731646498484
30-31	18.257699807717135	31.20078272036092	33.572044924887386	16.969472547034563
32-33	16.596457375033122	32.4937660943477	34.154668804652836	16.75510772596634
34-35	17.443724987939856	31.539146209717416	34.99616113711875	16.020967665223978
36-37	18.342511780233668	30.801871248076264	33.02202487523314	17.833592096456925
38-39	16.00459312458171	33.08068883279599	33.938842138520755	16.975875904101535
40-41	16.632807669572426	31.033299587577034	35.89098988306756	16.442902859782986
42-43	17.62207923684765	30.96059899849843	33.298908132274306	18.118413632379617
44-45	16.399078672908498	32.53147527840249	33.57476270391836	17.494683344770653
46-47	18.08309661986762	30.968577136877013	33.84413607513218	17.10419016812319
48-49	17.571079622086405	31.424853831963663	32.99201978576743	18.0120467601825
50-51	16.282553896955406	32.384035765972044	32.34768547143275	18.9857248656398
52-53	17.54326364494935	31.578214283287696	33.55403963880717	17.324482432955786
54-55	18.040617207617935	31.190591048994758	32.28721488799353	18.481576855393772
56-57	17.07512620686375	33.27071117482793	31.343126397108286	18.311036221200037
58-59	17.105361498583356	31.527935371214642	33.11375943578908	18.252943694412927
60-61	18.698742296481015	32.50901999687443	31.39060833169128	17.401629374953288
62-63	16.73258042157622	33.47127655237085	32.464223101072285	17.331919924980635
64-65	17.879190435258263	31.976584185831598	32.63570605882492	17.50851932008521
66-67	17.937140508053638	32.20572197364264	31.414797223609515	18.442340294694212
68-69	16.783478116113866	33.23197972474851	31.761956045374706	18.222586113762915
70-71	18.414714105660813	31.47572505495028	33.155997649113154	16.95356319027575
72-73	18.353258524686513	31.499468321833458	31.832064440073243	18.315208713406783
74-75	16.84145798723293	33.269464007256644	31.021127837173985	18.867950168336442
76-77	17.59368099201631	32.06658739595719	33.157125870562254	17.182605741464243
78-79	17.90133103228042	31.99029752885942	31.864939971055655	18.24343146780451
80-81	16.906284184564374	33.30808063650385	31.446062277906496	18.339572901025285
82-83	18.5237024303739	32.23591680878386	31.649555982850814	17.590824777991426
84-85	17.59524116891676	31.77865048682217	31.45727311640927	19.1688352278518
86-87	16.267661377171258	33.59797796553165	32.10047663211678	18.033884025180306
88-89	18.587672540606285	31.29766842986774	32.33996147456608	17.774697554959896
90-91	17.95105246708068	31.543776923181454	32.38221745097773	18.12295315876014
92-93	17.449160546001806	33.21024059138872	31.726672962854757	17.61392589975472
94-95	18.182466797810644	30.759038232206375	32.86787414255282	18.190620827430156
96-97	18.72583724580273	31.146427139741405	31.862561914403546	18.265173700052316
98-99	16.762921340680396	32.81650235427608	31.96821557423274	18.45236073081078
100-101	17.710746777733235	31.390687530150363	33.12395110715523	17.77461458496117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1995.0
1	1127.0
2	276.0
3	244.0
4	149.5
5	134.5
6	243.5
7	490.5
8	804.0
9	826.0
10	514.0
11	305.0
12	260.0
13	254.5
14	256.0
15	223.5
16	206.0
17	200.5
18	205.0
19	228.0
20	245.0
21	266.0
22	306.0
23	374.0
24	433.0
25	518.5
26	828.5
27	1297.0
28	1526.0
29	1802.5
30	2757.0
31	3631.5
32	5153.0
33	9143.0
34	12668.5
35	11874.0
36	11387.5
37	12120.0
38	10443.5
39	8855.5
40	7395.0
41	5830.0
42	4746.0
43	4155.0
44	4007.5
45	3916.0
46	3252.5
47	2311.5
48	1997.0
49	1529.5
50	1132.0
51	811.0
52	456.0
53	304.0
54	239.5
55	220.0
56	207.0
57	195.0
58	164.5
59	130.5
60	123.5
61	119.5
62	106.0
63	88.5
64	72.0
65	47.0
66	22.5
67	8.5
68	3.0
69	3.5
70	2.0
71	1.0
72	0.5
73	0.0
74	0.5
75	0.5
76	1.0
77	1.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09240448705318015
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.004416390925335816
20-21	0.001698611894359929
22-23	0.0
24-25	0.0
26-27	0.0013588895154879434
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0030575014098478724
38-39	0.001698611894359929
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.01698611894359929
48-49	0.001698611894359929
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.00407666854646383
62-63	0.011550560881647519
64-65	0.009172504229543618
66-67	0.006454725198567731
68-69	0.0030575014098478724
70-71	3.3972237887198585E-4
72-73	0.0023780566521039007
74-75	0.0023780566521039007
76-77	0.002717779030975887
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.3972237887198585E-4
88-89	0.0023780566521039007
90-91	6.794447577439717E-4
92-93	0.0
94-95	0.008493059471799645
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	147179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.475067774614587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.549693047950896	12.602341366635187
2	11.750456280073005	4.811827774342807
3	5.764061722249876	3.540586632603836
4	3.773021403683425	3.090114758219583
5	2.4323875891820146	2.490165037131656
6	1.9014435042309608	2.3359310771237745
7	1.4999170399867263	2.1497632135019265
8	1.207897793263647	1.9785431345504454
9	1.01543056246889	1.8711908628268978
>10	7.877882860461257	32.41019438914519
>50	0.8627841380454622	11.924255498406703
>100	0.3285216525634644	10.57759598855815
>500	0.016592002654720425	2.4792939210077525
>1k	0.019910403185664508	7.7381963459460925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA	3713	2.5227783855033667	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	2501	1.6992913391176732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1452	0.9865537882442469	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1430	0.9716060035738794	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1171	0.7956298113181907	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.7623370181887362	No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	992	0.6740091996820199	No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC	851	0.5782074888401199	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	725	0.4925974493643794	No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	560	0.3804890643366241	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	521	0.3539907187846092	No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG	452	0.30710903050027516	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	349	0.2371262204526461	No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC	281	0.19092397692605603	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	272	0.1848089741063603	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	264	0.1793734160444085	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	239	0.16238729710080924	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	235	0.15966951806983332	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	229	0.1555928495233695	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	228	0.1549134047656255	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	228	0.1549134047656255	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	223	0.1515161809769057	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	220	0.14947784670367376	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	216	0.1467600676726979	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.14200395436849006	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	209	0.14200395436849006	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	208	0.1413245096107461	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	208	0.1413245096107461	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	206	0.13996562009525815	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	202	0.13724784106428226	No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA	201	0.1365683963065383	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	198	0.1345300620333064	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	189	0.12841505921361063	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	189	0.12841505921361063	No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT	187	0.1270561696981227	No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT	179	0.12162061163617091	No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC	177	0.12026172212068298	No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG	175	0.11890283260519503	No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA	174	0.11822338784745105	No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG	173	0.1175439430897071	No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC	172	0.11686449833196312	No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA	171	0.11618505357421915	No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC	166	0.1127878297854993	No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC	165	0.11210838502775532	No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT	164	0.11142894027001135	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	163	0.11074949551226737	No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA	163	0.11074949551226737	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	162	0.11007005075452339	TruSeq Adapter, Index 1 (95% over 21bp)
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC	161	0.10939060599677944	No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG	158	0.10735227172354751	No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC	157	0.10667282696580356	No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC	155	0.1053139374503156	No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC	155	0.1053139374503156	No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA	154	0.10463449269257163	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	151	0.10259615841933971	RNA PCR Primer, Index 1 (95% over 23bp)
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT	151	0.10259615841933971	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	149	0.10123726890385178	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.1005578241461078	No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC	148	0.1005578241461078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	6.794447577439716E-4	0.0
14-15	0.0	0.0	0.0	0.0010191671366159573	0.0
16-17	0.0	0.0	0.0	0.0027177790309758864	0.0
18-19	0.0	0.0	0.0	0.004076668546463829	0.0
20-21	0.0	0.0	0.0	0.0047561133042078015	0.0
22-23	0.0	0.0	0.0	0.005435558061951774	0.0
24-25	0.0	0.0	0.0	0.011550560881647517	0.0
26-27	0.0	0.0	0.0	0.01630667418585532	0.0
28-29	0.0	0.0	0.0	0.02072306511119113	0.0
30-31	0.0	0.0	0.0	0.02819695744637482	0.0
32-33	0.0	0.0	0.0	0.0407666854646383	0.0
34-35	0.0	0.0	0.0	0.06047058343921347	0.0
36-37	0.0	0.0	0.0	0.08934698564333227	0.0
38-39	0.0	0.0	0.0	0.11448644167985922	0.0
40-41	0.0	0.0	0.0	0.14336284388397802	0.0
42-43	0.0	0.0	0.0	0.16578452088952908	0.0
44-45	0.0	0.0	0.0	0.18480897410636027	0.0
46-47	0.0	0.0	0.0	0.22047982388791879	0.0
48-49	0.0	0.0	0.0	0.24527955754557376	0.0
50-51	0.0	0.0	0.0	0.25920817507932514	0.0
52-53	0.0	0.0	0.0	0.2778929059172844	0.0
54-55	0.0	0.0	0.0	0.30337208433268337	0.0
56-57	0.0	0.0	0.0	0.3298704298846982	0.0
58-59	0.0	0.0	0.0	0.359086554467689	0.0
60-61	0.0	0.0	0.0	0.3876232342929358	0.0
62-63	0.0	0.0	0.0	0.4120832455717188	0.0
64-65	0.0	0.0	0.0	0.43892131350260566	0.0
66-67	0.0	0.0	0.0	0.4715346618743163	0.0
68-69	0.0	0.0	0.0	0.5068657892770028	0.0
70-71	0.0	0.0	0.0	0.5428763614374333	0.0
72-73	0.0	0.0	0.0	0.576848599324632	0.0
74-75	0.0	0.0	0.0	0.6104811148329585	0.0
76-77	0.0	0.0	0.0	0.6529464121919568	0.0
78-79	0.0	0.0	0.0	0.7056033809171145	0.0
80-81	0.0	0.0	0.0	0.7619972958098642	0.0
82-83	0.0	0.0	0.0	0.8119364855040461	0.0
84-85	0.0	0.0	0.0	0.8611962304404841	0.0
86-87	0.0	0.0	0.0	0.9138531991656418	0.0
88-89	0.0	0.0	0.0	0.9875729553808628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	490	0.0	52.379356	1
GGATATG	40	5.356401E-4	47.529408	1
GATATGA	45	9.600566E-4	42.219654	2
GTATCAA	895	0.0	40.891228	1
TGGGGAG	60	8.2584906E-5	39.58093	6
GATTGGC	50	0.0016117215	37.99769	9
ATATGAG	55	0.0025731202	34.54335	3
CCTAGCC	70	2.045404E-4	33.926506	3
TCTAGGC	35	2.0503675E-4	33.926506	38-39
AGATTGG	70	2.045404E-4	33.926506	8
TGAGATT	70	2.045404E-4	33.926506	6
GAGATTG	70	2.045404E-4	33.926506	7
AACGCAG	1140	0.0	32.914665	6
CAACGCA	1115	0.0	32.800697	5
TCAACGC	1115	0.0	32.800697	4
ATCAACG	1125	0.0	32.509132	3
CGCAGAG	1195	0.0	31.399765	8
ACGCAGA	1205	0.0	31.139185	7
CATGGGG	270	0.0	29.905588	4
TATCAAC	1230	0.0	29.733965	2
>>END_MODULE