FastQCFastQC Report
Fri 10 Feb 2017
SRR2031475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729744
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11134595639585973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.048.2772671
GTATCAA29150.039.72941
TCAACGC33000.034.244214
ATCAACG33700.033.1102263
CAACGCA34100.033.0003175
AACGCAG35700.031.512196
TATCAAC37150.030.671772
ACGCAGA40650.027.4413647
GTACATG47850.026.8811651
TACATGG46250.026.5873132
CGCAGAG42400.026.196818
ACATGGG49250.024.2950883
GTAGGAC3200.022.2569393
TACACCG1605.006171E-520.7731445
GCAGAGT53150.020.182089
CATGGGG36800.019.6119124
GTATTAC3707.2759576E-1219.2420021
CAGAGTA48150.018.92520310-11
GTATAAA11800.018.5027621
AGAGTAC43800.018.36670110-11
Produced by FastQC (version 0.11.5)