FastQCFastQC Report
Fri 10 Feb 2017
SRR2031475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729744
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.13921135150635006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.10307883709959392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.053.790041
GTATCAA31150.045.3163531
TCAACGC35600.038.4257854
ATCAACG36700.037.274063
CAACGCA37500.036.4788785
AACGCAG39200.034.8968856
TATCAAC40100.034.3505672
ACGCAGA43350.031.0082767
CGCAGAG46250.028.9612718
TACATGG50400.026.8592952
GTACATG52750.026.3998661
ACATGGG49800.025.6568433
GCAGAGT57500.022.7166929
CATGGGG36250.021.6200264
AGAGTAC48250.020.42683410-11
CAGAGTA52250.020.3630110-11
GTATATA9450.019.1120971
TATACGC2001.2856874E-518.9994163
GAGTACT29450.018.87038812-13
TACACCG1650.001464518817.2721965
Produced by FastQC (version 0.11.5)