FastQCFastQC Report
Fri 10 Feb 2017
SRR2031473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680872
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA62190.9133875383331963No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT52470.7706294281450845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.40345321881352153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20380.2993220458470902No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT20340.2987345639121597No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC14080.20679364109553633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.19019727643374965No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA8970.13174282390816483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11000.066.88331
GTATCAA24150.046.9742051
ATCAACG29250.037.9780433
TCAACGC29400.037.7842754
CAACGCA29900.037.1524355
AACGCAG30600.036.588596
TATCAAC30550.036.517352
CATTCCG851.6451286E-533.487869
ACGCAGA34300.032.5034037
CGCAGAG34800.031.9000748
GTACATG33300.028.7929291
GCAGAGT38950.028.6230229
ACATGGG31400.028.2718723
TACATGG34700.027.498512
CCGATTG350.00833904227.1781782-83
CATGGGA19900.025.2868864
GAGTACT21150.025.23464812-13
AGAGTAC36650.023.55876410-11
CAGAGTA38600.023.5362110-11
GTACTTT23700.022.21844914-15
Produced by FastQC (version 0.11.5)