##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031473_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680872
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9743373203774	34.0	33.0	34.0	31.0	34.0
2	33.0344646277127	34.0	33.0	34.0	31.0	34.0
3	33.205615739816004	34.0	34.0	34.0	31.0	34.0
4	36.174009211716736	37.0	37.0	37.0	35.0	37.0
5	36.17197652422188	37.0	37.0	37.0	35.0	37.0
6	36.25621409016673	37.0	37.0	37.0	35.0	37.0
7	36.29279365284518	37.0	37.0	37.0	35.0	37.0
8	36.37628364802782	37.0	37.0	37.0	35.0	37.0
9	38.336979637876134	39.0	39.0	39.0	38.0	39.0
10-11	38.42491687130621	39.0	39.0	39.0	37.5	39.0
12-13	38.481243170522504	39.0	39.0	39.0	38.0	39.0
14-15	40.11978830088475	41.0	40.0	41.0	39.0	41.0
16-17	40.131077500616854	41.0	40.0	41.0	39.0	41.0
18-19	40.17877001844693	41.0	41.0	41.0	39.0	41.0
20-21	40.13063321740356	41.0	40.5	41.0	39.0	41.0
22-23	40.06987363263579	41.0	40.0	41.0	39.0	41.0
24-25	39.96540612626162	41.0	40.0	41.0	38.0	41.0
26-27	39.89079738923028	41.0	40.0	41.0	38.0	41.0
28-29	39.824598456097476	41.0	40.0	41.0	38.0	41.0
30-31	39.66606058113713	41.0	40.0	41.0	37.5	41.0
32-33	39.488845921112926	41.0	40.0	41.0	37.0	41.0
34-35	39.41494803722286	41.0	40.0	41.0	37.0	41.0
36-37	39.30283885958007	41.0	40.0	41.0	36.0	41.0
38-39	39.14383173342419	41.0	40.0	41.0	36.0	41.0
40-41	39.060701570926696	41.0	40.0	41.0	35.0	41.0
42-43	39.19774788800244	41.0	40.0	41.0	36.5	41.0
44-45	39.14785818773572	41.0	40.0	41.0	36.5	41.0
46-47	38.967422658003265	41.0	40.0	41.0	36.0	41.0
48-49	38.76531565404363	41.0	40.0	41.0	36.0	41.0
50-51	37.79483221515938	40.0	38.5	40.5	34.0	40.5
52-53	37.86702860449542	39.5	38.5	40.5	35.0	41.0
54-55	38.34227652187195	40.5	39.0	41.0	35.0	41.0
56-57	38.387961320189405	41.0	39.0	41.0	35.0	41.0
58-59	38.44504840851144	41.0	39.0	41.0	35.0	41.0
60-61	38.29625171838465	41.0	39.0	41.0	35.0	41.0
62-63	38.10422663878086	41.0	38.5	41.0	35.0	41.0
64-65	37.89240635537958	40.5	37.5	41.0	35.0	41.0
66-67	37.67201985101458	40.0	37.0	41.0	34.0	41.0
68-69	37.363911278478184	39.0	37.0	41.0	34.0	41.0
70-71	37.01828610957713	39.0	36.0	41.0	34.0	41.0
72-73	36.6580678894124	39.0	36.0	41.0	34.0	41.0
74-75	36.26314711135133	37.0	35.0	40.0	33.5	41.0
76-77	35.86464636524927	37.0	35.0	39.0	33.0	41.0
78-79	35.43032611122208	36.5	35.0	39.0	32.5	41.0
80-81	34.99711252628982	36.0	35.0	38.0	32.0	39.5
82-83	34.623897002667164	36.0	35.0	37.0	32.0	39.0
84-85	34.26739313703604	35.0	35.0	37.0	31.5	39.0
86-87	33.98609430260019	35.0	35.0	36.5	31.0	37.5
88-89	33.74991701817669	35.0	35.0	36.0	31.0	37.0
90-91	33.494390281873834	35.0	35.0	36.0	31.0	37.0
92-93	33.40281066044719	35.0	35.0	35.5	31.0	36.0
94-95	33.27998214054918	35.0	35.0	35.0	31.0	36.0
96-97	33.171035818773575	35.0	35.0	35.0	30.0	36.0
98-99	33.074189421800284	35.0	35.0	35.0	30.0	36.0
100-101	32.74800182706882	35.0	34.5	35.0	29.0	35.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.0026722617447418884
1101	2	0.13381376292706904
1101	3	0.06801748435336918
1101	4	0.12145586786056839
1101	5	0.09334782367913164
1101	6	0.16984181169259216
1101	7	0.23171751582601274
1101	8	0.1992396102710643
1101	9	0.2046259228833449
1101	10-11	0.045330836909904804
1101	12-13	-0.017834905735739426
1101	14-15	-0.035896919193454835
1101	16-17	0.07108550280933912
1101	18-19	-0.023369791656172367
1101	20-21	-0.03481877351553919
1101	22-23	-0.04852777777030326
1101	24-25	0.11654278900665105
1101	26-27	-0.027913288957194027
1101	28-29	-0.04872913608883067
1101	30-31	0.09818388375843057
1101	32-33	0.11131281054640851
1101	34-35	-0.03545896657886516
1101	36-37	0.02373384524079114
1101	38-39	0.07105879377901658
1101	40-41	0.17664464764241927
1101	42-43	0.2230297173513165
1101	44-45	0.1008885783793616
1101	46-47	0.11985511585634612
1101	48-49	0.16469722645297935
1101	50-51	0.11330374276287358
1101	52-53	0.07182001668236637
1101	54-55	0.2072014559475619
1101	56-57	0.41229611717263737
1101	58-59	0.22737963840024378
1101	60-61	0.1510540858311984
1101	62-63	0.23740642676433055
1101	64-65	0.18245742228219797
1101	66-67	-0.030596839587538227
1101	68-69	-0.17529130054294484
1101	70-71	-0.0019690336605009406
1101	72-73	-0.006832428266029922
1101	74-75	-0.03439505072475413
1101	76-77	0.1542284934256486
1101	78-79	-0.06497567078231725
1101	80-81	-0.08393321698083156
1101	82-83	-0.0519184690892871
1101	84-85	-0.024565948415414596
1101	86-87	0.1278563530599044
1101	88-89	0.11877394015463238
1101	90-91	0.21770146594931816
1101	92-93	0.0029997805763315455
1101	94-95	-0.005920962959194753
1101	96-97	0.02494762625728697
1101	98-99	0.10647719819387191
1101	100-101	-0.07198556818060808
1102	1	0.100205527370548
1102	2	0.06090193763543539
1102	3	0.13035144312063096
1102	4	0.08033789036471006
1102	5	-0.02399563135561067
1102	6	-0.02200861205324145
1102	7	0.12361785952777637
1102	8	0.07733450409074294
1102	9	0.09553647435540569
1102	10-11	0.23565640682939915
1102	12-13	0.18624160376914034
1102	14-15	0.2892785178930595
1102	16-17	0.26077559742773104
1102	18-19	0.2791006723698999
1102	20-21	0.3522201124087587
1102	22-23	0.30543626838557714
1102	24-25	0.3589916784310745
1102	26-27	0.40733421647949086
1102	28-29	0.36911954332750696
1102	30-31	0.320112515435234
1102	32-33	0.4564877885988423
1102	34-35	0.3516487744570611
1102	36-37	0.43199014691798254
1102	38-39	0.5073276500382278
1102	40-41	0.20703700702019745
1102	42-43	0.3390878769689394
1102	44-45	0.3936791612952675
1102	46-47	0.3084550045405834
1102	48-49	0.33268505057407083
1102	50-51	0.3706898879668259
1102	52-53	0.36841341003440675
1102	54-55	0.45594771912327303
1102	56-57	0.49283651504043036
1102	58-59	0.6321745362364695
1102	60-61	0.573095754649593
1102	62-63	0.5367927555819207
1102	64-65	0.5085121965235047
1102	66-67	0.2760422276552106
1102	68-69	0.31844001533561084
1102	70-71	0.5184594172716217
1102	72-73	0.44296645742874574
1102	74-75	0.5709733528121035
1102	76-77	0.37356358256433
1102	78-79	0.5311919684628066
1102	80-81	0.44516810905385285
1102	82-83	0.5317433605057857
1102	84-85	0.40585596128110524
1102	86-87	0.49193062477365146
1102	88-89	0.4339797308695239
1102	90-91	0.47309941980491743
1102	92-93	0.4135010457890118
1102	94-95	0.3680670103479713
1102	96-97	0.42505607305810855
1102	98-99	0.4003006077842457
1102	100-101	0.41350034808009895
1103	1	-0.05347713515984509
1103	2	0.14549341783874326
1103	3	0.017515393120362432
1103	4	-0.1899244017257331
1103	5	-0.04043249501417279
1103	6	0.0316976658894248
1103	7	0.020083061681077652
1103	8	0.1147783503973443
1103	9	0.04462915948145252
1103	10-11	0.0913756303117097
1103	12-13	-0.01705086774477138
1103	14-15	-0.04301045961326366
1103	16-17	-0.04096496579985143
1103	18-19	0.07064501299002046
1103	20-21	0.018731579301558554
1103	22-23	-0.032516382712806546
1103	24-25	-0.01602241030919771
1103	26-27	0.09688546997483627
1103	28-29	0.024411005819253262
1103	30-31	0.008104615006395477
1103	32-33	0.15035288018907522
1103	34-35	0.09828089463803735
1103	36-37	-0.05877569016787021
1103	38-39	-0.04842887506119098
1103	40-41	-0.16026986989519543
1103	42-43	-0.16429342069796604
1103	44-45	-0.09779111280453634
1103	46-47	-0.18835691480204986
1103	48-49	-0.2935701890955258
1103	50-51	-0.24310151280282355
1103	52-53	-0.4238273168909217
1103	54-55	-0.2973423371780157
1103	56-57	-0.19889916409052688
1103	58-59	-0.20740569981030887
1103	60-61	-0.21448897905980147
1103	62-63	-0.2620160078828917
1103	64-65	-0.27431725189561007
1103	66-67	-0.4751519608065138
1103	68-69	-0.42660477344062997
1103	70-71	-0.46478303250537323
1103	72-73	-0.34000253660835966
1103	74-75	-0.28470964248091946
1103	76-77	-0.4433981873034796
1103	78-79	-0.28505597189754894
1103	80-81	-0.091606186643304
1103	82-83	-0.28391419454764844
1103	84-85	-0.21276854829655178
1103	86-87	-0.2617686758881135
1103	88-89	-0.29636580554920045
1103	90-91	-0.05514514999204323
1103	92-93	-0.17218667258647002
1103	94-95	-0.33498393990402064
1103	96-97	-0.3059429125525881
1103	98-99	-0.15753434195249838
1103	100-101	-0.33229315660809533
1104	1	-0.04340500968441319
1104	2	0.010225714023853527
1104	3	0.07311179575096816
1104	4	0.06091706215428161
1104	5	-0.07316245061947768
1104	6	-0.1327026241389362
1104	7	-0.08072723687005379
1104	8	-0.09618421304636371
1104	9	0.03162876514463875
1104	10-11	0.09163385732250617
1104	12-13	0.10591225540279936
1104	14-15	0.057072223912363995
1104	16-17	0.03375804438930885
1104	18-19	0.04981639685782824
1104	20-21	0.14613671585658494
1104	22-23	0.08891391507508928
1104	24-25	0.16539160518441776
1104	26-27	0.16633963150028563
1104	28-29	0.15486652844274573
1104	30-31	0.267691451789112
1104	32-33	0.25561536858158007
1104	34-35	0.26201971646140976
1104	36-37	0.1716035092011836
1104	38-39	0.2138178271381932
1104	40-41	0.1811011501650981
1104	42-43	0.41444037651115195
1104	44-45	0.11195027856839346
1104	46-47	0.08993929574836557
1104	48-49	0.2977136952492998
1104	50-51	0.24360170422234972
1104	52-53	0.2360279233365219
1104	54-55	0.39722090299854784
1104	56-57	0.30385228923144325
1104	58-59	0.33244267132423744
1104	60-61	0.3633696448319199
1104	62-63	0.4701631482363311
1104	64-65	0.4285346937113488
1104	66-67	0.5294795886827544
1104	68-69	0.5338610528222105
1104	70-71	0.5424803972072709
1104	72-73	0.43076565694741475
1104	74-75	0.4693488465730553
1104	76-77	0.34886899496917323
1104	78-79	0.4538879686720776
1104	80-81	0.46937482158688226
1104	82-83	0.3779279944032936
1104	84-85	0.37166023574680196
1104	86-87	0.49565312457745847
1104	88-89	0.38592338326180453
1104	90-91	0.35022291890241064
1104	92-93	0.33746185882691293
1104	94-95	0.3952349806307609
1104	96-97	0.34171968778068873
1104	98-99	0.2866466459248258
1104	100-101	0.3376482016914153
1105	1	0.027606498492453113
1105	2	-0.12159742346857882
1105	3	-0.005810069785070482
1105	4	1.538883202414354E-4
1105	5	-0.0734410696382568
1105	6	-0.026015767688306823
1105	7	-0.028710277451523325
1105	8	0.11720161372529248
1105	9	0.06906062462374507
1105	10-11	0.1396047840335939
1105	12-13	0.17545192832829315
1105	14-15	0.16113642742538303
1105	16-17	0.24210450108221693
1105	18-19	0.20112469485903972
1105	20-21	0.1625267824828711
1105	22-23	0.2394408684366809
1105	24-25	0.2368270988246408
1105	26-27	0.18565519782372775
1105	28-29	0.17369148301572324
1105	30-31	0.2563225844359991
1105	32-33	0.30930404211276397
1105	34-35	0.3474346165219728
1105	36-37	0.30786032325327284
1105	38-39	0.4048717338613912
1105	40-41	0.27500787336315824
1105	42-43	0.3380503098848635
1105	44-45	0.41208958807778373
1105	46-47	0.44508163372534426
1105	48-49	0.6808935862244638
1105	50-51	0.5363939513453104
1105	52-53	0.49917752963573037
1105	54-55	0.6766939496369488
1105	56-57	0.6267414473174497
1105	58-59	0.5769611449765719
1105	60-61	0.4785059258737121
1105	62-63	0.5374980967523797
1105	64-65	0.6459847239960297
1105	66-67	0.6433741980104415
1105	68-69	0.6275557832885923
1105	70-71	0.5240612572920682
1105	72-73	0.6840909143671539
1105	74-75	0.5916020264640309
1105	76-77	0.575186378373175
1105	78-79	0.6019739771517862
1105	80-81	0.6257891159539355
1105	82-83	0.5073707563657379
1105	84-85	0.5699218953470435
1105	86-87	0.606101337781574
1105	88-89	0.5566017600031827
1105	90-91	0.7505923919490485
1105	92-93	0.7316048024974151
1105	94-95	0.6793294688197307
1105	96-97	0.614608845866691
1105	98-99	0.5393601528902963
1105	100-101	0.6087929624424788
1106	1	0.0337603446462964
1106	2	0.1384048625069667
1106	3	0.14013803857017137
1106	4	-0.014650089277196798
1106	5	0.0338329045302217
1106	6	-0.11961046422504751
1106	7	0.07517359499104259
1106	8	0.04959388712796908
1106	9	0.16630602409797035
1106	10-11	0.14392527780431408
1106	12-13	0.049758706245775386
1106	14-15	0.025510641621288244
1106	16-17	0.13476423362308054
1106	18-19	0.07471139048139719
1106	20-21	0.10228675505116769
1106	22-23	0.03359105702966758
1106	24-25	0.011251604482431787
1106	26-27	-0.005864975910419901
1106	28-29	0.10152094924043809
1106	30-31	0.14557049848331616
1106	32-33	0.045175570287419475
1106	34-35	0.21627905588647423
1106	36-37	0.11133043412309007
1106	38-39	-0.009012138581176998
1106	40-41	0.14281447983247375
1106	42-43	0.3584389257266736
1106	44-45	0.4085268951985981
1106	46-47	0.1791929607341487
1106	48-49	0.40192799015634506
1106	50-51	0.4111567813830206
1106	52-53	0.2342741121022982
1106	54-55	0.27171166594737883
1106	56-57	0.2033433446771511
1106	58-59	0.4375052071551053
1106	60-61	0.34186059497874766
1106	62-63	0.232636969766439
1106	64-65	0.3601360555767812
1106	66-67	0.385715036963461
1106	68-69	0.3701423646121711
1106	70-71	0.1744315853295575
1106	72-73	0.25673235910370096
1106	74-75	0.23132542342368367
1106	76-77	0.3164310920547351
1106	78-79	0.4452920706481933
1106	80-81	0.5057228226632731
1106	82-83	0.2755739795912433
1106	84-85	0.31946241426349076
1106	86-87	0.3901623733284936
1106	88-89	0.4231453649866026
1106	90-91	0.561131882176511
1106	92-93	0.4290250791004553
1106	94-95	0.4726132726830343
1106	96-97	0.19678280860529185
1106	98-99	0.3282537407289112
1106	100-101	0.4833912022413145
1107	1	0.04241835330430632
1107	2	0.09731255544140538
1107	3	0.05200988803488826
1107	4	0.09191546008181462
1107	5	0.1164956602984688
1107	6	0.0817364800639453
1107	7	-0.036471405212651575
1107	8	-0.05090805438437229
1107	9	0.07647543203854923
1107	10-11	0.19302913745794825
1107	12-13	0.058259121135492364
1107	14-15	0.1875888638778207
1107	16-17	0.0850482240259467
1107	18-19	0.03450353823788532
1107	20-21	0.17022575018184227
1107	22-23	0.05244120143701281
1107	24-25	0.16251696451450215
1107	26-27	0.2599131620816877
1107	28-29	0.14969103901528769
1107	30-31	0.19524255335596763
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2316	72-73	-0.9719060240178408
2316	74-75	-0.7807745521812066
2316	76-77	-0.8360841918526276
2316	78-79	-0.6653700718340474
2316	80-81	-0.7297868503682992
2316	82-83	-0.6551742111046082
2316	84-85	-0.7156081467501636
2316	86-87	-0.8709939549853587
2316	88-89	-0.8637847688908096
2316	90-91	-0.7641148411163883
2316	92-93	-0.8168414278891092
2316	94-95	-0.7173869490907165
2316	96-97	-0.8146755016266098
2316	98-99	-0.8659173059692478
2316	100-101	-0.8634917677526985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	259.0
3	74.0
4	68.0
5	99.0
6	104.0
7	167.0
8	325.0
9	336.0
10	235.0
11	234.0
12	311.0
13	347.0
14	380.0
15	427.0
16	536.0
17	827.0
18	1236.0
19	1857.0
20	2568.0
21	3711.0
22	4923.0
23	4757.0
24	3747.0
25	2865.0
26	2433.0
27	2266.0
28	2391.0
29	2704.0
30	3223.0
31	4017.0
32	5161.0
33	6635.0
34	10178.0
35	17068.0
36	33450.0
37	103575.0
38	289986.0
39	166652.0
40	740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71897868138457	21.460122374847913	15.061304979045806	21.75959396472172
2	13.57794065989303	23.551146927539342	42.50003306960932	20.370879342958304
3	15.92205902882376	30.18155367625989	32.51419880531656	21.382188489599788
4	11.520147435313344	19.243894338025434	43.14187709608867	26.094081130572544
5	10.589548058729873	40.66898069535533	37.15824339641781	11.583227849496986
6	24.968435967270413	41.3720322827667	20.961898280770704	12.697633469192182
7	23.505621149104893	34.995935013135856	25.962843226761578	15.535600610997664
8	22.928607347764586	36.52370687753824	23.962575981696755	16.58510979300042
9	25.740782129476603	16.645247995859382	22.919105062256286	34.69486481240773
10-11	21.666874875992974	28.139784393118806	32.54120707519786	17.65213365569036
12-13	23.21501389018488	26.82587417057485	31.054206621000375	18.904905318239894
14-15	20.516175604678498	28.165304395703757	29.51319181593574	21.805328183682004
16-17	19.420446179144705	30.455598841421388	30.187409348277487	19.93654563115642
18-19	19.090354148654136	29.62754559725443	32.27974886158371	19.00235139250772
20-21	20.53294955910777	29.247798110073557	31.773479173416142	18.44577315740253
22-23	20.167822032503118	29.086702415390764	31.644382511347867	19.10109304075825
24-25	19.791105696368298	29.09144146927869	31.779096149941292	19.33835668441172
26-27	19.34528385869785	29.699127805387672	31.581537872963438	19.374050462951043
28-29	19.74642437185209	28.919108330373405	32.15399560680702	19.18047169096748
30-31	20.41054700371887	28.86178682389493	31.566207103516454	19.161459068869746
32-33	19.51487141369502	29.355210238332663	31.654713849935423	19.475204498036895
34-35	20.16523879065216	29.137866977623606	31.839734558523297	18.857159673200933
36-37	20.33213733679503	28.70215124880725	31.282237281102837	19.683474133294883
38-39	19.395028663238413	29.70901053868047	31.44926544241629	19.446695355664826
40-41	20.02852433284469	28.93645723078598	32.009111898795254	19.025906537574077
42-43	20.01400917431867	28.968782179389464	31.128811638345745	19.888397007946125
44-45	20.033570191316286	29.650386446696665	30.760231081675954	19.5558122803111
46-47	20.252407247208726	29.082251012252875	30.74125059992841	19.92409114060999
48-49	20.050544935285718	29.159074632089638	30.796748182691193	19.993632249933455
50-51	19.80794418056012	29.60943894491375	30.27511871117573	20.307498163350402
52-53	20.37060058007943	29.53167744170702	30.434162161844075	19.66355981636948
54-55	20.617289312680953	29.04691690290874	30.162622393761	20.173171390649305
56-57	20.180188532774697	29.86201096230301	29.924555985521934	20.033244519400363
58-59	20.256145491345123	29.2936803520787	30.530059660394677	19.920114496181498
60-61	20.797053382050986	29.364950235698515	30.00471912378902	19.833277258461475
62-63	20.000368119388483	29.686988083975397	30.19602357436564	20.116620222270488
64-65	20.305554982812808	29.298859783738884	30.41227168428587	19.98331354916244
66-67	20.49478955999912	29.515212131463407	29.680166498746114	20.309831809791362
68-69	20.204736743406176	29.589018933000933	30.01974562342118	20.186498700171718
70-71	20.515615255174684	28.93860098139584	30.458793730363258	20.086990033066215
72-73	20.575152725520702	28.936530179151358	30.04343620468346	20.44488089064448
74-75	19.92826384076809	29.84465502351728	29.526506040049256	20.70057509566538
76-77	20.284884400865273	29.438667695424066	30.24973355526641	20.026714348444248
78-79	20.495301274674564	29.450986393857075	29.550096682196642	20.50361564927172
80-81	20.045174423057663	29.93059606082566	29.403070152181982	20.621159363934698
82-83	20.65468307364135	29.6094529158816	29.621532754777796	20.114331255699252
84-85	20.771806053253794	29.332428397704486	29.271477744178036	20.624287804863688
86-87	19.67569008062474	29.925529596758523	29.82195124824057	20.576829074376175
88-89	20.769825910218877	29.307211155173192	29.846507419924517	20.07645551468342
90-91	20.692047632536394	29.422251325271954	29.620757014468545	20.264944027723107
92-93	20.346217166969012	30.031455562764737	29.460459121745387	20.161868148520867
94-95	20.56578168270769	29.437123722449222	29.793716233859367	20.203378360983717
96-97	20.601009870728898	29.332067440420424	29.629983717930426	20.436938970920256
98-99	20.030759635003317	29.992850701763935	29.439515612978774	20.536874050253974
100-101	20.394672466143764	29.738309877185383	29.895607940830033	19.971409715840828
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	390.0
1	265.0
2	152.5
3	164.5
4	148.0
5	153.5
6	193.5
7	305.0
8	499.5
9	581.5
10	493.5
11	443.5
12	474.0
13	531.5
14	593.0
15	646.0
16	697.5
17	747.0
18	809.5
19	880.0
20	990.0
21	1166.5
22	1344.5
23	1638.5
24	2175.0
25	2860.5
26	4072.0
27	5898.5
28	7909.0
29	10863.0
30	14340.5
31	17609.5
32	21513.5
33	28988.0
34	36734.5
35	36118.0
36	34595.5
37	35540.0
38	32667.0
39	29916.5
40	27852.5
41	26159.5
42	25497.0
43	24282.5
44	23261.5
45	22187.5
46	20925.5
47	19308.0
48	18100.0
49	17694.5
50	16582.0
51	15184.5
52	13949.0
53	12640.5
54	11509.5
55	10143.5
56	8493.0
57	6893.5
58	5786.0
59	5003.0
60	4036.0
61	2856.0
62	1840.5
63	1283.0
64	896.5
65	671.5
66	515.0
67	392.5
68	273.0
69	175.5
70	139.0
71	105.0
72	79.0
73	60.0
74	47.5
75	33.0
76	18.5
77	19.0
78	16.0
79	9.5
80	5.5
81	4.0
82	4.5
83	4.5
84	5.0
85	3.0
86	1.0
87	1.5
88	2.5
89	2.5
90	1.0
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04993596446909258
2	0.07181966654525374
3	0.0524327626925472
4	0.06432927187488985
5	0.09884383555205678
6	0.07475707621990624
7	0.09884383555205678
8	0.11367775440905192
9	0.11455897731144768
10-11	0.06858851590313599
12-13	0.05221245696694827
14-15	0.10229529191977346
16-17	0.05625139526959546
18-19	0.031063107309450236
20-21	0.038700372463546746
22-23	0.08342243476013113
24-25	0.18300062273085102
26-27	0.1857177266799046
28-29	0.14048161769025602
30-31	0.16097005017095725
32-33	0.21509182342642963
34-35	0.18351466942391523
36-37	0.18263344652151947
38-39	0.22287595906425878
40-41	0.1617778378314867
42-43	0.08863633693263932
44-45	0.07438990001057467
46-47	0.08555205677425419
48-49	0.12968663713590806
50-51	0.14180345204384964
52-53	0.1670651752458612
54-55	0.2438784382380242
56-57	0.19996416360196925
58-59	0.15039537534220823
60-61	0.2526906672619817
62-63	0.2562155588715647
64-65	0.27582276844987014
66-67	0.1449611674441011
68-69	0.1431252863974433
70-71	0.15039537534220823
72-73	0.16735891621332644
74-75	0.17022289064611262
76-77	0.15972165105922995
78-79	0.19475026142946106
80-81	0.28610370231115395
82-83	0.3018188440705448
84-85	0.23998637041910958
86-87	0.24615493073587985
88-89	0.2428503448518958
90-91	0.19012384119188333
92-93	0.17374778225569565
94-95	0.16221844928268456
96-97	0.1443736855091706
98-99	0.15935447484989837
100-101	0.18659894958230036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	680872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.09652389831973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.83301661695144	28.371741739309275
2	15.727686761707288	13.5561725678241
3	6.8159500967106466	8.812312686979352
4	3.685181893535078	6.352741181775586
5	2.2052043427032295	4.75183208279941
6	1.3772307927230594	3.5612315863254693
7	0.9400185312985574	2.835807176928018
8	0.6368733257386237	2.1957621202317568
9	0.4660401547397502	1.8076239599686443
>10	2.113159803159883	15.606365805236832
>50	0.12666667895801678	3.7838437023257767
>100	0.06987317622735541	5.035679600347414
>500	6.884056771166221E-4	0.2155372333138012
>1k	0.0017210141927915552	1.412488324579501
>5k	6.884056771166221E-4	1.7008602320550892
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA	6219	0.9133875383331963	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	5247	0.7706294281450845	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2747	0.40345321881352153	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2038	0.2993220458470902	No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT	2034	0.2987345639121597	No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC	1408	0.20679364109553633	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1295	0.19019727643374965	No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	897	0.13174282390816483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	5.874819349305009E-4	0.0
24-25	0.0	0.0	0.0	0.0012483991117273143	0.0
26-27	0.0	0.0	0.0	0.0019827515303904404	0.0
28-29	0.0	0.0	0.0	0.0031577154002514424	0.0
30-31	0.0	0.0	0.0	0.006315430800502885	0.0
32-33	0.0	0.0	0.0	0.011088721521813205	0.0
34-35	0.0	0.0	0.0	0.018946292401508656	0.0
36-37	0.0	0.0	0.0	0.03187089496997968	0.0
38-39	0.0	0.0	0.0	0.04472206229658438	0.0
40-41	0.0	0.0	0.0	0.059115369702381654	0.0
42-43	0.0	0.0	0.0	0.07262745420578318	0.0
44-45	0.0	0.0	0.0	0.09025191225369819	0.0
46-47	0.0	0.0	0.0	0.10684827691548485	0.0
48-49	0.0	0.0	0.0	0.12674922746125555	0.0
50-51	0.0	0.0	0.0	0.14598926083022945	0.0
52-53	0.0	0.0	0.0	0.16354028363627818	0.0
54-55	0.0	0.0	0.0	0.18204596458658895	0.0
56-57	0.0	0.0	0.0	0.20694051157926893	0.0
58-59	0.0	0.0	0.0	0.23161475284634997	0.0
60-61	0.0	0.0	0.0	0.2526172320201154	0.0
62-63	0.0	0.0	0.0	0.2736931464357471	0.0
64-65	0.0	0.0	0.0	0.2953565427863093	0.0
66-67	0.0	0.0	0.0	0.3239228518723049	0.0
68-69	0.0	0.0	0.0	0.35550000587481934	0.0
70-71	0.0	0.0	0.0	0.3894270876170558	0.0
72-73	0.0	0.0	0.0	0.42379478081049005	0.0
74-75	0.0	0.0	0.0	0.46080614271111164	0.0
76-77	0.0	0.0	0.0	0.502003313398113	0.0
78-79	0.0	0.0	0.0	0.5443020127131091	0.0
80-81	0.0	0.0	0.0	0.5918146142006133	0.0
82-83	0.0	0.0	0.0	0.6416771434278395	0.0
84-85	0.0	0.0	0.0	0.687574169594285	0.0
86-87	0.0	0.0	0.0	0.7381710512401745	0.0
88-89	0.0	0.0	0.0	0.7940552703004382	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1100	0.0	66.8833	1
GTATCAA	2415	0.0	46.974205	1
ATCAACG	2925	0.0	37.978043	3
TCAACGC	2940	0.0	37.784275	4
CAACGCA	2990	0.0	37.152435	5
AACGCAG	3060	0.0	36.58859	6
TATCAAC	3055	0.0	36.51735	2
CATTCCG	85	1.6451286E-5	33.48786	9
ACGCAGA	3430	0.0	32.503403	7
CGCAGAG	3480	0.0	31.900074	8
GTACATG	3330	0.0	28.792929	1
GCAGAGT	3895	0.0	28.623022	9
ACATGGG	3140	0.0	28.271872	3
TACATGG	3470	0.0	27.49851	2
CCGATTG	35	0.008339042	27.17817	82-83
CATGGGA	1990	0.0	25.286886	4
GAGTACT	2115	0.0	25.234648	12-13
AGAGTAC	3665	0.0	23.558764	10-11
CAGAGTA	3860	0.0	23.53621	10-11
GTACTTT	2370	0.0	22.218449	14-15
>>END_MODULE