FastQCFastQC Report
Fri 10 Feb 2017
SRR2031473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680872
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA65400.9605329636113689No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT47650.6998378549859592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.5099343195196748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25800.3789258480301731No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT19600.28786614811594546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.2213338189850662No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC14280.2097310507701888No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA9800.14393307405797273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.068.856981
GTATCAA27000.044.735191
TCGCTCG250.001615792338.00061436-37
CGCCCGC250.001615792338.00061466-67
GCCCGCG250.001615792338.00061466-67
GTGACGC500.001615807637.9978266
ATCAACG32150.037.377333
TCAACGC32350.037.1462484
CAACGCA32700.036.748665
AACGCAG33250.036.1407856
TATCAAC33300.035.943892
ACGCAGA36750.032.5695657
CGCAGAG37250.032.1323938
TAGTCGC300.003950891631.66718332-33
TACTGCG300.003952312331.66485624-25
CGTCGCC300.003952312331.66485690-91
CATGGGA20850.029.1590044
GCAGAGT41400.028.5672059
ACATGGG34450.028.5397323
TACATGG35500.027.9631882