##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031473_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680872 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.32302841062637 34.0 33.0 34.0 31.0 34.0 2 33.4085202505023 34.0 34.0 34.0 31.0 34.0 3 33.49879713073823 34.0 34.0 34.0 31.0 34.0 4 36.664490535666026 37.0 37.0 37.0 35.0 37.0 5 36.59772761987569 37.0 37.0 37.0 35.0 37.0 6 36.64804838501216 37.0 37.0 37.0 37.0 37.0 7 36.659385317651484 37.0 37.0 37.0 37.0 37.0 8 36.66570809197617 37.0 37.0 37.0 37.0 37.0 9 38.60110270359186 39.0 39.0 39.0 38.0 39.0 10-11 38.61121620510169 39.0 39.0 39.0 38.5 39.0 12-13 38.60971298570069 39.0 39.0 39.0 38.5 39.0 14-15 40.30918880494424 41.0 41.0 41.0 39.0 41.0 16-17 40.36925883279089 41.0 41.0 41.0 39.0 41.0 18-19 40.424403558965565 41.0 41.0 41.0 39.5 41.0 20-21 40.425279641401026 41.0 41.0 41.0 39.0 41.0 22-23 40.378476424349955 41.0 41.0 41.0 39.0 41.0 24-25 40.3496090307723 41.0 41.0 41.0 39.0 41.0 26-27 40.27308877439519 41.0 41.0 41.0 39.0 41.0 28-29 40.20472056421765 41.0 40.0 41.0 39.0 41.0 30-31 40.04199174000399 41.0 40.0 41.0 38.0 41.0 32-33 39.894668160829056 41.0 40.0 41.0 38.0 41.0 34-35 39.82474312352395 41.0 40.0 41.0 38.0 41.0 36-37 39.73555308486764 41.0 40.0 41.0 37.0 41.0 38-39 39.63629727760871 41.0 40.0 41.0 37.0 41.0 40-41 39.55000719665371 41.0 40.0 41.0 37.0 41.0 42-43 39.350823649672776 41.0 40.0 41.0 36.5 41.0 44-45 39.202480054988314 41.0 40.0 41.0 36.0 41.0 46-47 39.015094613965616 41.0 40.0 41.0 36.0 41.0 48-49 38.773016807858156 41.0 40.0 41.0 35.0 41.0 50-51 38.67955210377281 41.0 39.5 41.0 35.0 41.0 52-53 38.71330073787731 41.0 40.0 41.0 35.0 41.0 54-55 38.67338574651329 41.0 39.0 41.0 35.0 41.0 56-57 38.63359853247013 41.0 39.0 41.0 35.0 41.0 58-59 38.62200604518911 41.0 39.0 41.0 35.0 41.0 60-61 38.49346352912148 41.0 39.0 41.0 35.0 41.0 62-63 38.332156411190354 41.0 39.0 41.0 35.0 41.0 64-65 38.11346405785522 40.5 38.0 41.0 35.0 41.0 66-67 37.8599611968182 40.0 37.0 41.0 35.0 41.0 68-69 37.518311811911786 39.0 37.0 41.0 35.0 41.0 70-71 37.18630080250033 39.0 36.0 41.0 34.0 41.0 72-73 36.833626584732514 39.0 36.0 41.0 34.0 41.0 74-75 36.4377518828796 37.0 35.0 40.0 34.0 41.0 76-77 35.1799097334007 36.5 34.5 39.0 32.0 39.5 78-79 35.53770091882174 36.5 35.0 39.0 33.0 40.5 80-81 35.275430624258306 36.0 35.0 38.0 33.5 39.5 82-83 34.92807899282097 36.0 35.0 37.0 33.0 39.0 84-85 34.579760953600676 35.0 35.0 37.0 33.0 39.0 86-87 34.3243083868921 35.0 35.0 36.5 33.0 37.5 88-89 34.09244615728066 35.0 35.0 36.0 33.0 37.0 90-91 33.885238341421 35.0 35.0 36.0 33.0 37.0 92-93 33.75425924402825 35.0 35.0 36.0 32.5 36.5 94-95 33.63748472546969 35.0 35.0 35.0 32.0 36.0 96-97 33.53257513893948 35.0 35.0 35.0 32.0 36.0 98-99 33.45458691207745 35.0 35.0 35.0 32.0 36.0 100-101 33.21229173765407 35.0 34.5 35.0 31.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07693667192486231 1101 2 0.03964004031166013 1101 3 0.052138626836409685 1101 4 0.05924746517543156 1101 5 0.05430011169411131 1101 6 0.07537987450889005 1101 7 0.038277836404176924 1101 8 0.04100311969443737 1101 9 0.009097071283434843 1101 10-11 -0.01846381573542999 1101 12-13 0.13010782586957959 1101 14-15 0.04810426300431203 1101 16-17 -0.004827363915161698 1101 18-19 0.03789393129606822 1101 20-21 -0.024506480267277198 1101 22-23 0.07331184767490129 1101 24-25 -0.013729084560289095 1101 26-27 -0.02857995307072514 1101 28-29 -0.08802653247168735 1101 30-31 -0.026417794051646126 1101 32-33 0.04330036580888219 1101 34-35 0.07974851594857313 1101 36-37 0.09565716171224636 1101 38-39 0.10171505658528446 1101 40-41 0.040876590228144494 1101 42-43 0.10347397715534612 1101 44-45 0.25829402841668525 1101 46-47 0.2564901969405966 1101 48-49 0.22848011541426416 1101 50-51 0.16078264829383926 1101 52-53 0.19511398175156103 1101 54-55 0.19654547591876081 1101 56-57 0.234393526377616 1101 58-59 0.2402203236131797 1101 60-61 0.1669113443148902 1101 62-63 0.21846538148754036 1101 64-65 0.3031241037424337 1101 66-67 0.20607949394793224 1101 68-69 0.23937195294811886 1101 70-71 0.30548731082323144 1101 72-73 0.29459256668463496 1101 74-75 0.21064927611312356 1101 76-77 0.19168790419676185 1101 78-79 0.2916569888235969 1101 80-81 0.18867447666048776 1101 82-83 0.3086480594444154 1101 84-85 0.20718683633719337 1101 86-87 0.21004016886529797 1101 88-89 0.13019136109075902 1101 90-91 0.0903179962653553 1101 92-93 0.06450088149912148 1101 94-95 0.05651587832316807 1101 96-97 -0.00653950479352261 1101 98-99 0.04629837859501862 1101 100-101 0.11381419153790517 1102 1 0.19272514902193194 1102 2 0.19663830172009256 1102 3 0.1451790964282722 1102 4 0.029476236283827006 1102 5 0.17201653191686006 1102 6 0.10833565231344267 1102 7 0.03600848668332901 1102 8 0.12318743335242743 1102 9 0.12660405720830425 1102 10-11 0.14199584951396105 1102 12-13 0.18668234144518436 1102 14-15 0.13853541076164788 1102 16-17 0.13756644141996333 1102 18-19 0.10071417928922699 1102 20-21 0.13710077017597655 1102 22-23 0.08592954688134569 1102 24-25 0.11975372174455146 1102 26-27 0.05507900756136053 1102 28-29 0.05914983415883057 1102 30-31 0.05232663298775719 1102 32-33 0.13558056020981724 1102 34-35 0.181027212750827 1102 36-37 0.01684531404270473 1102 38-39 0.0384572917369681 1102 40-41 0.14096897083624782 1102 42-43 0.23439479951489517 1102 44-45 0.24101139539720862 1102 46-47 0.2148715854039196 1102 48-49 0.3953633887862722 1102 50-51 0.2530728840652188 1102 52-53 0.2910793591062628 1102 54-55 0.38933978848653794 1102 56-57 0.40865146910164185 1102 58-59 0.2739751076089547 1102 60-61 0.19354105873831884 1102 62-63 0.14336884088310597 1102 64-65 0.2267939090545994 1102 66-67 0.35148427593544085 1102 68-69 0.27997925503270693 1102 70-71 0.3158227499753963 1102 72-73 0.3196816479853126 1102 74-75 0.2955060287913014 1102 76-77 0.14056011484749575 1102 78-79 0.30108296670962886 1102 80-81 0.27989889268307167 1102 82-83 0.2200444411082998 1102 84-85 0.20231677167077322 1102 86-87 0.23234492158938025 1102 88-89 0.24826066376062528 1102 90-91 0.16267611170094654 1102 92-93 0.1217309551167105 1102 94-95 0.10895908720088698 1102 96-97 0.16941399505441979 1102 98-99 0.14174734261249 1102 100-101 0.16845359158036644 1103 1 -0.06711147526223016 1103 2 0.052179067825534275 1103 3 0.05279989731277368 1103 4 0.06016822153492285 1103 5 -0.022147777681198022 1103 6 -0.0552252298295457 1103 7 -0.044860882516807976 1103 8 -0.024811428814075498 1103 9 -0.013199183470320008 1103 10-11 0.02648025529115472 1103 12-13 0.04777407122045929 1103 14-15 0.05393990520396841 1103 16-17 0.04078216284396774 1103 18-19 0.02263169709470958 1103 20-21 -0.004944592884143617 1103 22-23 -0.003366230790284419 1103 24-25 0.011856781550584117 1103 26-27 -0.0223983296935657 1103 28-29 -0.01782165023085014 1103 30-31 0.15203734418737014 1103 32-33 0.17711416655162537 1103 34-35 0.11618814641834518 1103 36-37 0.1597655207849087 1103 38-39 0.08361103343802512 1103 40-41 0.13069358292626276 1103 42-43 0.20511571364603753 1103 44-45 0.22038816319867038 1103 46-47 0.32773581288474674 1103 48-49 0.28997826971629337 1103 50-51 0.23641999305571204 1103 52-53 0.46120559421915175 1103 54-55 0.513114068082281 1103 56-57 0.4543929125400439 1103 58-59 0.5066258302942401 1103 60-61 0.4782651072787232 1103 62-63 0.43499100502095445 1103 64-65 0.46418113204313727 1103 66-67 0.35725931766494057 1103 68-69 0.2290343701846851 1103 70-71 0.2602795686573316 1103 72-73 0.36421011797494884 1103 74-75 0.29394284951275296 1103 76-77 0.17590809331453983 1103 78-79 0.37548627501420384 1103 80-81 0.30454747924697756 1103 82-83 0.3431303851075711 1103 84-85 0.35330110499157996 1103 86-87 0.3548458474208829 1103 88-89 0.37343845024508937 1103 90-91 0.3812770058663304 1103 92-93 0.4802907369961744 1103 94-95 0.4010991802670816 1103 96-97 0.37714106529417535 1103 98-99 0.445217466432382 1103 100-101 0.4163510148168612 1104 1 -0.03586982311818332 1104 2 0.08446889266366497 1104 3 0.013356039426675181 1104 4 0.058929240704785 1104 5 0.048237527548216974 1104 6 0.10721864077709142 1104 7 0.09835970440902031 1104 8 0.10520014687951118 1104 9 0.06307827700059221 1104 10-11 -0.05051337171710202 1104 12-13 -0.016743247798025607 1104 14-15 0.10425728273694546 1104 16-17 -0.021165606608917642 1104 18-19 0.1036793609670923 1104 20-21 0.10833183722283479 1104 22-23 0.03386425271753524 1104 24-25 0.03378699665495333 1104 26-27 0.05418696790352584 1104 28-29 0.08589402020533754 1104 30-31 0.1264156276773818 1104 32-33 0.1615098771847272 1104 34-35 0.15939982896448868 1104 36-37 0.2321237821411728 1104 38-39 0.2425198653106193 1104 40-41 0.25116856797963294 1104 42-43 0.3412420540731915 1104 44-45 0.42593304763254025 1104 46-47 0.31009878084169173 1104 48-49 0.43429791150581565 1104 50-51 0.5103059915546879 1104 52-53 0.4031375901994636 1104 54-55 0.45991294358633183 1104 56-57 0.5782541059813369 1104 58-59 0.4431605001325636 1104 60-61 0.4883588576762392 1104 62-63 0.4953805080293634 1104 64-65 0.5673320103965835 1104 66-67 0.5268864785670857 1104 68-69 0.5591222691326934 1104 70-71 0.6933975008059576 1104 72-73 0.6327509445558022 1104 74-75 0.4983377969133045 1104 76-77 0.46983785047938653 1104 78-79 0.6348797595523834 1104 80-81 0.4367304515289163 1104 82-83 0.43743921443605416 1104 84-85 0.538876009905529 1104 86-87 0.46970725711139494 1104 88-89 0.44483376654137885 1104 90-91 0.394483472990089 1104 92-93 0.28280558822942936 1104 94-95 0.23953302708630986 1104 96-97 0.3109370023529294 1104 98-99 0.4705555148467653 1104 100-101 0.6649612955592303 1105 1 -0.06874686733380742 1105 2 -0.06093389352652423 1105 3 -0.048012827926442014 1105 4 0.02195770830017807 1105 5 -0.06219251485033084 1105 6 -0.0522725766668799 1105 7 -0.03329383889806792 1105 8 -0.04209240309020146 1105 9 -0.012475932569365966 1105 10-11 0.03550722186254518 1105 12-13 0.11580066498211039 1105 14-15 0.032833902967119855 1105 16-17 -0.024981475779121354 1105 18-19 0.012480087799318085 1105 20-21 -0.012170888398003399 1105 22-23 0.029987814195422402 1105 24-25 0.028366039659189823 1105 26-27 -0.056188562078297366 1105 28-29 -0.027182902871579984 1105 30-31 -0.04540145287986519 1105 32-33 -0.07016183692793021 1105 34-35 0.002113335929969651 1105 36-37 -0.012073673946048302 1105 38-39 0.12905529838148055 1105 40-41 -0.05747467853763055 1105 42-43 -0.076504766035832 1105 44-45 -0.011183851216642893 1105 46-47 0.015362257522497202 1105 48-49 0.007471745545792885 1105 50-51 0.013001327714590616 1105 52-53 -0.10500293069696198 1105 54-55 -0.1097478680429802 1105 56-57 -0.1988082804511322 1105 58-59 -0.13330830665120175 1105 60-61 -0.12200455843520075 1105 62-63 -0.12118520971745994 1105 64-65 -0.09413376791958683 1105 66-67 -0.027874272492880436 1105 68-69 -0.027909832623819852 1105 70-71 -0.022756345347893614 1105 72-73 -0.009229673251589077 1105 74-75 -0.09095959860456304 1105 76-77 -0.2030815922825795 1105 78-79 -0.11089249968444648 1105 80-81 -0.06472926985206584 1105 82-83 -0.15985333553366132 1105 84-85 -0.2465025485439014 1105 86-87 -0.16043810933318525 1105 88-89 -0.24555993424602462 1105 90-91 -0.37324517631336107 1105 92-93 -0.3290357200685676 1105 94-95 -0.3760575240947901 1105 96-97 -0.5138903749941335 1105 98-99 -0.3846595063525058 1105 100-101 -0.1769284682202965 1106 1 -0.05231984710292181 1106 2 0.05322086701753648 1106 3 0.022290063832613782 1106 4 0.029291117832698887 1106 5 0.08423336239278711 1106 6 0.036546716966675774 1106 7 0.08153528443030922 1106 8 0.09666859816708495 1106 9 0.09039982466003238 1106 10-11 0.09695653035494445 1106 12-13 0.16689564726579675 1106 14-15 0.10087367894151811 1106 16-17 0.03547114737781243 1106 18-19 0.10817445286961203 1106 20-21 0.09258965566417743 1106 22-23 0.099961525476715 1106 24-25 0.06831917716062463 1106 26-27 0.1094840862243629 1106 28-29 0.09570085527220584 1106 30-31 0.19656105712127925 1106 32-33 0.020718263654984526 1106 34-35 0.07604407087109877 1106 36-37 0.09793089800503196 1106 38-39 0.15400329243794886 1106 40-41 0.07330941107260713 1106 42-43 0.2577505774319917 1106 44-45 0.17201651453945033 1106 46-47 0.07107033844602739 1106 48-49 0.2742576639364671 1106 50-51 0.2053604544719363 1106 52-53 0.13151095660444412 1106 54-55 0.24479436535511923 1106 56-57 0.43398060970774566 1106 58-59 0.5114339496066549 1106 60-61 0.4005454472797325 1106 62-63 0.2340953937800876 1106 64-65 0.40094224076712237 1106 66-67 0.3724446210949637 1106 68-69 0.2092269830122504 1106 70-71 0.45238709961724766 1106 72-73 0.5733729099007618 1106 74-75 0.5413149470577991 1106 76-77 0.3278259400068251 1106 78-79 0.4870764110482142 1106 80-81 0.3806576315080932 1106 82-83 0.26724347553046357 1106 84-85 0.37534451969246874 1106 86-87 0.4330960088517557 1106 88-89 0.1455544788297587 1106 90-91 0.35219544547198467 1106 92-93 0.30767133216597387 1106 94-95 0.37285778051144547 1106 96-97 0.3176000810579822 1106 98-99 0.36329340255137055 1106 100-101 0.37891105629679345 1107 1 -0.09488517347211456 1107 2 -0.07865617124880231 1107 3 -0.027779727693342693 1107 4 0.03056687690934723 1107 5 -0.0669344395922522 1107 6 -0.06467350906780922 1107 7 -0.06867623428046699 1107 8 -0.02524036623816528 1107 9 -0.06548292557635449 1107 10-11 0.03794034429567006 1107 12-13 0.08829816747961416 1107 14-15 -0.025387875254658354 1107 16-17 0.008862458064818668 1107 18-19 0.004813310132540494 1107 20-21 -0.010999837226947307 1107 22-23 -0.033537549329935246 1107 24-25 0.03769559898875485 1107 26-27 0.001117465227729042 1107 28-29 -0.025072790761477393 1107 30-31 0.044356566878157366 1107 32-33 0.1107139539478581 1107 34-35 0.0751092289258608 1107 36-37 0.06192233004995984 1107 38-39 0.1988821382083259 1107 40-41 0.0991387080757562 1107 42-43 0.29039500086393844 1107 44-45 0.26904924901646154 1107 46-47 0.07974121190145667 1107 48-49 0.2830861311601822 1107 50-51 0.29521355992682174 1107 52-53 0.3037727380787061 1107 54-55 0.46018109188597833 1107 56-57 0.3865030748602294 1107 58-59 0.33903601569311803 1107 60-61 0.2907926443619928 1107 62-63 0.263968525436276 1107 64-65 0.24349748971167173 1107 66-67 0.26055840593979696 1107 68-69 0.21846712375313615 1107 70-71 0.27030947771793734 1107 72-73 0.14043066640874713 1107 74-75 0.19559495794999293 1107 76-77 0.10653212733114259 1107 78-79 0.22414246535052484 1107 80-81 0.3092622941394936 1107 82-83 0.30800880232848016 1107 84-85 0.2607834987421498 1107 86-87 0.14609202719694991 1107 88-89 0.11161178292569218 1107 90-91 0.25163882857064124 1107 92-93 0.3467385057056589 1107 94-95 0.4151690271317534 1107 96-97 0.4381142870105279 1107 98-99 0.358424076731076 1107 100-101 0.4901422388504102 1108 1 0.048658445799659944 1108 2 0.09963156043698973 1108 3 0.02412629508646802 1108 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-0.2031936515679078 2316 16-17 -0.181544812939471 2316 18-19 -0.25226924247240134 2316 20-21 -0.23434846206811244 2316 22-23 -0.3196868892106224 2316 24-25 -0.2135589507732618 2316 26-27 -0.21432480394588538 2316 28-29 -0.2733099598941564 2316 30-31 -0.6070508979621465 2316 32-33 -0.8248614159558656 2316 34-35 -0.8021193468101799 2316 36-37 -0.4283729468124804 2316 38-39 -0.5926859951508519 2316 40-41 -0.5542178855793409 2316 42-43 -0.4536574640840598 2316 44-45 -0.3759982408071494 2316 46-47 -0.47624509035350826 2316 48-49 -0.45055733979672397 2316 50-51 -0.46443839674005716 2316 52-53 -0.370016325262597 2316 54-55 -0.37265257527604234 2316 56-57 -0.46658860957474246 2316 58-59 -0.3520798336316844 2316 60-61 -0.6094787260586259 2316 62-63 -0.5956015893577202 2316 64-65 -0.3658367912643996 2316 66-67 -0.3147361171159204 2316 68-69 -0.450577264694239 2316 70-71 -0.47476170317414557 2316 72-73 -0.43864796640122705 2316 74-75 -0.4209672526420647 2316 76-77 -0.2407509378623729 2316 78-79 -0.455052469068292 2316 80-81 -0.452394788413109 2316 82-83 -0.31090959270932217 2316 84-85 -0.26583664728098455 2316 86-87 -0.33957703011389384 2316 88-89 -0.32794799394751806 2316 90-91 -0.3060963052838943 2316 92-93 -0.21987001015659047 2316 94-95 -0.32660478777638957 2316 96-97 -0.2604384040794727 2316 98-99 -0.5559262495595476 2316 100-101 -0.5333463786680568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 8.0 15 45.0 16 156.0 17 412.0 18 805.0 19 1471.0 20 2204.0 21 3454.0 22 5043.0 23 5344.0 24 4034.0 25 3063.0 26 2321.0 27 2089.0 28 2284.0 29 2675.0 30 3026.0 31 3614.0 32 4391.0 33 5916.0 34 8689.0 35 14015.0 36 26325.0 37 91321.0 38 302872.0 39 184922.0 40 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.782217517861866 21.476434094851665 15.17156214166005 21.569786245626414 2 13.56757423634019 23.62608795269554 42.354148980856735 20.45218883010754 3 15.855843682806753 30.455944729699564 32.626102997332836 21.062108590160854 4 11.317692605952367 19.38073529238976 43.108396291814024 26.19317580984385 5 10.502739142567599 40.74610424897557 37.126911157783425 11.624245450673403 6 24.681291050300203 41.79493355579316 20.843565310366706 12.680210083539931 7 23.54040113266517 35.156681432045964 25.695725481441446 15.607191953847419 8 23.032669870401485 36.496727725622435 23.736326357964494 16.734276046011583 9 25.799709783924147 16.587699303246424 22.782696307088557 34.829894605740876 10-11 21.65164671186361 28.295847090201974 32.30460350844211 17.7479026894923 12-13 23.424960932451327 26.900430624258302 30.901035730651284 18.773572712639087 14-15 20.50928808939125 28.12929596164918 29.580302905685652 21.78111304327392 16-17 19.280496187242242 30.58357518006321 30.25039948771575 19.885529144978793 18-19 19.04611181693337 29.5341083506525 32.46583289882425 18.953946933589876 20-21 20.399311739136504 29.131676523269196 32.13661229072926 18.33239944686504 22-23 20.04569140748922 28.98841485624317 31.91003595389442 19.05585778237319 24-25 19.683875970813897 28.888689797788718 32.18703368621415 19.24040054518324 26-27 19.133858209892363 29.590803375444384 32.08970733272773 19.18563108193553 28-29 19.631590078605083 28.648262815918415 32.61582206347155 19.10432504200496 30-31 20.324157844646276 28.71332644021196 31.887344464157728 19.07517125098403 32-33 19.5044736749345 29.233321387867324 31.939556921124673 19.322648016073508 34-35 19.993478950522274 28.954267468775335 32.32377010656922 18.72848347413317 36-37 20.219694838904402 28.57083058249682 31.72459273544162 19.484881843157158 38-39 19.281293419224454 29.609377524373514 31.841841665418247 19.26748739098378 40-41 19.90888155189228 28.789772526994795 32.323696671327355 18.977649249785568 42-43 20.03878849475379 28.784485189580423 31.368304174646628 19.808422141019165 44-45 19.831994853658248 29.543952460961826 31.066265024850487 19.55778766052944 46-47 20.209638894030338 29.020098657053627 30.86639284780431 19.90386960111173 48-49 20.070895215490907 29.103756403628335 30.93782311886074 19.887525262020024 50-51 19.82663408100201 29.475731121268023 30.447352806401202 20.250281991328766 52-53 20.3613895122725 29.45237871435453 30.597307570292216 19.588924203080754 54-55 20.547768156129198 29.11832179910468 30.288438943002504 20.04547110176362 56-57 20.203650612743658 29.76256917599784 30.073787731027267 19.959992480231232 58-59 20.219879801196114 29.302130209496056 30.59025778707305 19.88773220223478 60-61 20.688340901029516 29.606178706316843 29.989667395897364 19.71581299675628 62-63 19.918568126853938 29.892577199809935 30.222255842083268 19.96659883125286 64-65 20.236642152002666 29.540831089705456 30.304031773589674 19.918494984702203 66-67 20.533413430291777 29.602778326526806 29.66725735725569 20.196550885925728 68-69 20.162369373802058 29.70397514889367 30.05661998516571 20.077035492138563 70-71 20.510801980699707 28.967255887867076 30.520047498019082 20.001894633414135 72-73 20.459302146259358 29.07062989547028 30.11320993039675 20.35685802787361 74-75 19.9101572138602 29.90375351028705 29.53443900031725 20.651650275535502 76-77 20.238009561507223 29.48057956539939 30.202172268691573 20.07923860440182 78-79 20.411398911983454 29.52001257211341 29.59866171615223 20.46992679975091 80-81 20.009340962765396 29.91450669141924 29.47235310014217 20.603799245673198 82-83 20.632879601452256 29.609236390980975 29.669232983585754 20.088651023981015 84-85 20.667394165129423 29.34692570703451 29.390693111186835 20.594987016649238 86-87 19.578553908969408 29.906590097962898 29.879639285032383 20.63521670803531 88-89 20.68912592483798 29.272870807248157 29.88092309386991 20.15708017404395 90-91 20.61511053146014 29.44991661397669 29.66119058057381 20.27378227398936 92-93 20.213490935153743 29.97215335628429 29.648744551105054 20.165611157456908 94-95 20.50550657881208 29.33483348070421 29.820422959305077 20.339236981178637 96-97 20.55775534902302 29.436222961143944 29.568920443196372 20.437101246636665 98-99 19.92202646018635 29.899232161111044 29.53602145484026 20.64271992386234 100-101 20.314978439412986 29.807878720229354 29.839529309473733 20.037613530883924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 278.0 1 166.0 2 77.0 3 99.5 4 77.0 5 76.0 6 120.0 7 243.5 8 461.5 9 577.5 10 504.5 11 429.5 12 466.0 13 519.5 14 591.5 15 670.0 16 743.5 17 809.5 18 868.5 19 976.5 20 1118.5 21 1260.5 22 1433.5 23 1789.5 24 2347.5 25 3010.5 26 4156.5 27 5975.0 28 7880.5 29 10740.5 30 14289.5 31 17577.5 32 21423.5 33 28897.5 34 36927.5 35 36347.5 36 34553.5 37 35336.0 38 32580.0 39 30195.5 40 28108.5 41 26248.5 42 25690.0 43 24391.0 44 23273.0 45 22352.5 46 20967.5 47 19371.5 48 18250.0 49 17696.0 50 16687.5 51 15238.0 52 13826.0 53 12530.0 54 11407.5 55 10036.0 56 8419.5 57 6943.5 58 5840.5 59 4982.0 60 3969.0 61 2739.5 62 1687.0 63 1142.5 64 796.0 65 572.0 66 434.0 67 301.0 68 187.5 69 104.5 70 72.5 71 48.5 72 37.5 73 25.0 74 13.0 75 12.5 76 8.5 77 2.5 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09575955539367165 2 8.812229023957513E-4 3 0.0 4 0.0 5 2.9374096746525045E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.003965503060780881 20-21 0.003745197335181943 22-23 0.0 24-25 0.0 26-27 0.0019827515303904404 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.002717103949053567 38-39 0.001468704837326252 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.019093162885241276 48-49 0.0011749638698610018 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0025702334653209414 62-63 0.008445052814625952 64-65 0.008077876605294387 66-67 0.004479549753845069 68-69 0.002496798223454629 70-71 2.2030572559893783E-4 72-73 0.001909316288524128 74-75 0.0023499277397220036 76-77 0.002496798223454629 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 2.9374096746525045E-4 88-89 0.0013952695954599396 90-91 3.67176209331563E-4 92-93 0.0 94-95 0.007343524186631261 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 680872.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.146898985837225 #Duplication Level Percentage of deduplicated Percentage of total 1 64.8607858568789 27.336809896518954 2 16.08229776101975 13.556379583877112 3 7.200367014546534 9.104194236691415 4 3.7266130834386235 6.2826074074794835 5 2.222206084085384 4.68295476758298 6 1.4093440114846643 3.5639687813003267 7 0.9678141728924019 2.855325632737037 8 0.6723528933197433 2.2670071582066096 9 0.4700523642124699 1.7830124561265246 >10 2.174586813462176 15.701492395719441 >50 0.13863344983762665 4.069168697837601 >100 0.0710760185634071 5.087090365038869 >500 0.0014074459121465612 0.41004591025075837 >1k 0.002111168868219842 2.3300692013959914 >5k 3.518614780366403E-4 0.9698735092368751 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA 6540 0.9605329636113689 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 4765 0.6998378549859592 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.5099343195196748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2580 0.3789258480301731 No Hit CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT 1960 0.28786614811594546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1507 0.2213338189850662 No Hit TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC 1428 0.2097310507701888 No Hit AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA 980 0.14393307405797273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.874819349305009E-4 0.0 24-25 0.0 0.0 0.0 0.0012483991117273143 0.0 26-27 0.0 0.0 0.0 0.0019827515303904404 0.0 28-29 0.0 0.0 0.0 0.0030842801583851297 0.0 30-31 0.0 0.0 0.0 0.006168560316770259 0.0 32-33 0.0 0.0 0.0 0.01094185103808058 0.0 34-35 0.0 0.0 0.0 0.018652551434043402 0.0 36-37 0.0 0.0 0.0 0.031063107309450232 0.0 38-39 0.0 0.0 0.0 0.04362053366858969 0.0 40-41 0.0 0.0 0.0 0.05816071155811958 0.0 42-43 0.0 0.0 0.0 0.07159936081965479 0.0 44-45 0.0 0.0 0.0 0.08929725410943615 0.0 46-47 0.0 0.0 0.0 0.10545300732002491 0.0 48-49 0.0 0.0 0.0 0.1252805226239293 0.0 50-51 0.0 0.0 0.0 0.14452055599290323 0.0 52-53 0.0 0.0 0.0 0.1623653197664172 0.0 54-55 0.0 0.0 0.0 0.18079756547486164 0.0 56-57 0.0 0.0 0.0 0.20583898295127426 0.0 58-59 0.0 0.0 0.0 0.2312475766370184 0.0 60-61 0.0 0.0 0.0 0.25180944435958597 0.0 62-63 0.0 0.0 0.0 0.27317909974268295 0.0 64-65 0.0 0.0 0.0 0.2954299780281756 0.0 66-67 0.0 0.0 0.0 0.32451033380723543 0.0 68-69 0.0 0.0 0.0 0.3563812287772151 0.0 70-71 0.0 0.0 0.0 0.39060205148691673 0.0 72-73 0.0 0.0 0.0 0.42474943895475215 0.0 74-75 0.0 0.0 0.0 0.46212797706470526 0.0 76-77 0.0 0.0 0.0 0.5032517125098404 0.0 78-79 0.0 0.0 0.0 0.5458441527923017 0.0 80-81 0.0 0.0 0.0 0.5933567542798059 0.0 82-83 0.0 0.0 0.0 0.6434395892326311 0.0 84-85 0.0 0.0 0.0 0.6899975325758733 0.0 86-87 0.0 0.0 0.0 0.7405944142217626 0.0 88-89 0.0 0.0 0.0 0.7966989390076256 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1250 0.0 68.85698 1 GTATCAA 2700 0.0 44.73519 1 TCGCTCG 25 0.0016157923 38.000614 36-37 CGCCCGC 25 0.0016157923 38.000614 66-67 GCCCGCG 25 0.0016157923 38.000614 66-67 GTGACGC 50 0.0016158076 37.997826 6 ATCAACG 3215 0.0 37.37733 3 TCAACGC 3235 0.0 37.146248 4 CAACGCA 3270 0.0 36.74866 5 AACGCAG 3325 0.0 36.140785 6 TATCAAC 3330 0.0 35.94389 2 ACGCAGA 3675 0.0 32.569565 7 CGCAGAG 3725 0.0 32.132393 8 TAGTCGC 30 0.0039508916 31.667183 32-33 TACTGCG 30 0.0039523123 31.664856 24-25 CGTCGCC 30 0.0039523123 31.664856 90-91 CATGGGA 2085 0.0 29.159004 4 GCAGAGT 4140 0.0 28.567205 9 ACATGGG 3445 0.0 28.539732 3 TACATGG 3550 0.0 27.963188 2 >>END_MODULE