FastQCFastQC Report
Fri 10 Feb 2017
SRR2031472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490381
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.3173043001258205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.2540881477871288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.1317343045509512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.061.9854051
GTATCAA13050.042.1954081
TCAACGC15200.035.293684
TGGTATC2850.034.9813462
CAACGCA15750.034.6640555
ATCAACG15500.034.3042833
AACGCAG16400.033.283386
TATCAAC16600.032.031112
GTGGTAT3600.030.3279511
GGCCGTA650.00585929.2122061
ACGCAGA18700.029.18977
CGCAGAG18900.028.8808148
TACATGG16150.028.5142042
GTACATG16850.027.0451281
ACATGGG16800.025.998043
TAGCCCT5000.025.6363284
GTATAAT3150.025.618641
GTATAGC2059.604264E-1025.471621
CATGGGG10250.025.0110534
GCAGAGT22400.023.9443919
Produced by FastQC (version 0.11.5)