FastQCFastQC Report
Fri 10 Feb 2017
SRR2031472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490381
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.38154006782481376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.32933576137737797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.17170322667476923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.062.451811
GTATCAA15500.045.996481
ATCAACG18750.037.7433053
CAACGCA18800.037.642925
TCAACGC18900.037.4437524
TATCAAC19850.035.8910062
AACGCAG20000.035.8593036
ACTGCGT550.002579315634.5423778
ACGCAGA23350.030.3077937
CGCAGAG24050.029.4256528
ATAAACG650.005843390729.2281673
GTGGTAT3100.029.1311041
GCAGAGT26300.026.5470649
TACATGG15250.025.227262
TGGTATC3600.025.0672112
GTACATG16150.024.4270271
GAGTACT17300.023.74909412-13
ACATGGG16200.023.7478853
CAGAGTA25750.023.42509710-11
GTACTTT18350.022.52072514-15
Produced by FastQC (version 0.11.5)