##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	490381
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22847744916707	34.0	33.0	34.0	31.0	34.0
2	33.34010289958216	34.0	34.0	34.0	31.0	34.0
3	33.4297107759069	34.0	34.0	34.0	31.0	34.0
4	36.621019166729546	37.0	37.0	37.0	35.0	37.0
5	36.51790342611153	37.0	37.0	37.0	35.0	37.0
6	36.57520988782192	37.0	37.0	37.0	36.0	37.0
7	36.58973328901405	37.0	37.0	37.0	36.0	37.0
8	36.59523309426752	37.0	37.0	37.0	36.0	37.0
9	38.52180447448005	39.0	39.0	39.0	38.0	39.0
10-11	38.528855114696526	39.0	39.0	39.0	37.5	39.0
12-13	38.52873581969938	39.0	39.0	39.0	38.0	39.0
14-15	40.21615233869175	41.0	40.0	41.0	39.0	41.0
16-17	40.268003858224525	41.0	40.0	41.0	39.0	41.0
18-19	40.298262983272195	41.0	40.5	41.0	39.0	41.0
20-21	40.29696705214924	41.0	40.5	41.0	39.0	41.0
22-23	40.25374249817999	41.0	40.0	41.0	39.0	41.0
24-25	40.22790850379603	41.0	40.0	41.0	39.0	41.0
26-27	40.139153025912506	41.0	40.0	41.0	38.0	41.0
28-29	40.065169735369025	41.0	40.0	41.0	38.0	41.0
30-31	39.91761405927228	41.0	40.0	41.0	38.0	41.0
32-33	39.77170200313634	41.0	40.0	41.0	37.0	41.0
34-35	39.69452323805368	41.0	40.0	41.0	37.0	41.0
36-37	39.60542109094765	41.0	40.0	41.0	37.0	41.0
38-39	39.50196683803002	41.0	40.0	41.0	36.0	41.0
40-41	39.383542592392445	41.0	40.0	41.0	36.0	41.0
42-43	39.18436684944972	41.0	39.5	41.0	35.0	41.0
44-45	39.04517609776887	41.0	39.0	41.0	35.0	41.0
46-47	38.859965006800834	41.0	39.0	41.0	35.0	41.0
48-49	38.61847624602095	40.5	39.0	41.0	35.0	41.0
50-51	38.50169562034418	40.5	39.0	41.0	35.0	41.0
52-53	38.51627611999649	41.0	39.0	41.0	35.0	41.0
54-55	38.44154851024	40.0	38.5	41.0	35.0	41.0
56-57	38.3670187058634	40.5	38.0	41.0	35.0	41.0
58-59	38.334230934722186	41.0	38.0	41.0	35.0	41.0
60-61	38.149763143351805	40.0	37.0	41.0	35.0	41.0
62-63	37.95191391999282	40.0	37.0	41.0	35.0	41.0
64-65	37.68817001474363	39.5	36.0	41.0	34.5	41.0
66-67	37.40010114584374	39.0	36.0	41.0	34.0	41.0
68-69	37.05607170750906	39.0	35.0	41.0	34.0	41.0
70-71	36.70537500433336	37.5	35.0	40.5	34.0	41.0
72-73	36.36004453679894	37.0	35.0	39.5	34.0	41.0
74-75	35.97561385942767	36.5	35.0	39.0	33.5	41.0
76-77	34.75972865996032	35.5	34.5	38.5	31.0	39.0
78-79	35.18087772568676	36.0	35.0	37.0	33.0	39.0
80-81	34.9758004490386	35.0	35.0	37.0	33.0	39.0
82-83	34.68767957975533	35.0	35.0	37.0	33.0	39.0
84-85	34.41566455470338	35.0	35.0	36.0	33.0	37.0
86-87	34.20222133402395	35.0	35.0	36.0	33.0	37.0
88-89	34.01917896492727	35.0	35.0	36.0	32.0	37.0
90-91	33.86021991064091	35.0	35.0	35.0	32.0	36.0
92-93	33.76286601642396	35.0	35.0	35.0	32.0	36.0
94-95	33.67028188286251	35.0	35.0	35.0	32.0	36.0
96-97	33.5871149167688	35.0	35.0	35.0	32.0	36.0
98-99	33.53183443077933	35.0	35.0	35.0	32.0	36.0
100-101	33.260164035719164	35.0	34.5	35.0	31.0	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1227430940496319
1101	2	-0.05114410914464429
1101	3	0.0036186411743415192
1101	4	0.006498538964727629
1101	5	-0.12659595076380015
1101	6	-0.1612168843757047
1101	7	-0.1532236422006008
1101	8	-0.10769596700776418
1101	9	0.009666423665052548
1101	10-11	0.02384518221928289
1101	12-13	0.1281192540513274
1101	14-15	0.17500336191235277
1101	16-17	0.14724767102567426
1101	18-19	0.11805566127338096
1101	20-21	0.06745839574706736
1101	22-23	0.09808769834335607
1101	24-25	0.03817523781658139
1101	26-27	0.13718041776548517
1101	28-29	0.08728558987893109
1101	30-31	0.1704102630560982
1101	32-33	0.09809315193304968
1101	34-35	0.10171881685682393
1101	36-37	0.20952715499288388
1101	38-39	0.10877020267833615
1101	40-41	0.10406738919559899
1101	42-43	0.37897103702866985
1101	44-45	0.3068442400523992
1101	46-47	0.21763368419701834
1101	48-49	0.36784301163860533
1101	50-51	0.32011829671770897
1101	52-53	0.10444118647387768
1101	54-55	-0.016033907737671882
1101	56-57	0.1873554226919083
1101	58-59	0.2844053312715218
1101	60-61	0.1681050691581092
1101	62-63	0.12456771976020065
1101	64-65	0.12082776230418091
1101	66-67	0.08295418318876813
1101	68-69	0.3404611166754492
1101	70-71	0.185623995758462
1101	72-73	0.18539467918889585
1101	74-75	0.12808782713388922
1101	76-77	-0.047982077503526455
1101	78-79	0.31158223483098624
1101	80-81	0.20107278771148884
1101	82-83	0.2869747583873945
1101	84-85	0.353462332968256
1101	86-87	0.3521537704236266
1101	88-89	0.33031953970610317
1101	90-91	0.2754143706003944
1101	92-93	0.20206415782286768
1101	94-95	0.08737113654367334
1101	96-97	0.20469771727541541
1101	98-99	0.28109140973415236
1101	100-101	0.2203659658127748
1102	1	-0.12071926594495608
1102	2	0.07185157266171416
1102	3	0.06046271796535052
1102	4	-0.004224108140000737
1102	5	0.20718480986259635
1102	6	0.16720515401979696
1102	7	0.08715406967703387
1102	8	0.045901660587055915
1102	9	0.0967066400950003
1102	10-11	0.15165330874756222
1102	12-13	0.11454035597110135
1102	14-15	0.05137816030993747
1102	16-17	0.09030734534022145
1102	18-19	0.13739908773402476
1102	20-21	0.08870338820324264
1102	22-23	0.052616599024901234
1102	24-25	-0.008143892301525568
1102	26-27	-0.0420584355720095
1102	28-29	0.05393924993823873
1102	30-31	0.05126193570580284
1102	32-33	0.11521765117866067
1102	34-35	-0.0043578701001507625
1102	36-37	-0.1453139042652154
1102	38-39	-0.1598552590562221
1102	40-41	-0.11830657677911915
1102	42-43	-0.02108359072222754
1102	44-45	-0.16802439321330098
1102	46-47	-0.15625164955253013
1102	48-49	-0.3475099876850507
1102	50-51	-0.4966914426298743
1102	52-53	-0.22095291372902892
1102	54-55	-0.42835636754725215
1102	56-57	-0.42424126842718124
1102	58-59	-0.34389964765777137
1102	60-61	-0.2673613477816943
1102	62-63	-0.35578970180995384
1102	64-65	-0.23396907448891824
1102	66-67	-0.11790165157529486
1102	68-69	-0.2747592152531624
1102	70-71	-0.5144306319924397
1102	72-73	-0.39118562360492604
1102	74-75	-0.4679685695156053
1102	76-77	-0.3410313465321835
1102	78-79	-0.28805878280598307
1102	80-81	-0.08099370206532086
1102	82-83	-0.194553257844845
1102	84-85	-0.20127467343099426
1102	86-87	-0.19529953585595905
1102	88-89	-0.1361442238858004
1102	90-91	-0.049214941089942954
1102	92-93	-0.32823863599639225
1102	94-95	-0.24316577620800928
1102	96-97	0.026710099565619316
1102	98-99	-0.12489423097132857
1102	100-101	-0.025719045372909477
1103	1	0.10192231737980961
1103	2	0.027686484718259408
1103	3	0.0034965099850836623
1103	4	0.014940857421805731
1103	5	0.04889639059287276
1103	6	-0.01992127600971827
1103	7	-0.008788244510910204
1103	8	0.07556697126509704
1103	9	0.013075919365014954
1103	10-11	0.052551100493161584
1103	12-13	0.04588425328800838
1103	14-15	0.15260246628363205
1103	16-17	0.0666436305188185
1103	18-19	0.10015835324834654
1103	20-21	0.06126787109184306
1103	22-23	0.10564456567835379
1103	24-25	0.07762361194512124
1103	26-27	0.10735241919034877
1103	28-29	-0.011434577033696769
1103	30-31	0.16867243800902543
1103	32-33	0.21608460274979535
1103	34-35	0.24263276525638844
1103	36-37	0.08060750713780607
1103	38-39	0.20549556016650428
1103	40-41	0.31119425296843417
1103	42-43	0.23541854606128965
1103	44-45	0.39666409654825685
1103	46-47	0.22684695578625025
1103	48-49	0.4113369814111394
1103	50-51	0.15728179984731838
1103	52-53	0.3377168467456215
1103	54-55	0.3145981211667177
1103	56-57	0.25694475656803917
1103	58-59	0.29592382905790515
1103	60-61	0.3136650915488275
1103	62-63	0.28817516976274504
1103	64-65	0.322392568166471
1103	66-67	0.2915873316290529
1103	68-69	0.2609664344709799
1103	70-71	0.36570388991142977
1103	72-73	0.348060701274278
1103	74-75	0.2581168332913393
1103	76-77	0.14820641163189663
1103	78-79	0.16921288975948556
1103	80-81	0.2231785329661804
1103	82-83	0.2845855669976487
1103	84-85	0.37185325454985474
1103	86-87	0.3562451652635801
1103	88-89	0.3783628962782828
1103	90-91	0.5245518190763008
1103	92-93	0.4662262869765996
1103	94-95	0.3844170884036302
1103	96-97	0.3359073249289679
1103	98-99	0.34849510420261964
1103	100-101	0.3790092918521637
1104	1	-0.10953835363211795
1104	2	-0.09915819155650496
1104	3	-0.14225332788402056
1104	4	-0.012180491133946703
1104	5	0.01711407055816494
1104	6	-0.0407626770072298
1104	7	-0.040906490417981445
1104	8	0.019943245567233703
1104	9	-0.04120876848053001
1104	10-11	0.009995824357424965
1104	12-13	0.04092234821505514
1104	14-15	0.16858693904331545
1104	16-17	0.031903296552521
1104	18-19	0.08954869444638547
1104	20-21	0.10707509629747847
1104	22-23	0.1247153573903077
1104	24-25	0.16228393023488508
1104	26-27	0.10467434348571913
1104	28-29	0.08373275604147068
1104	30-31	0.08930995066456404
1104	32-33	0.12462411836335008
1104	34-35	5.981742228158282E-4
1104	36-37	0.05413468214119632
1104	38-39	-0.01865331098259304
1104	40-41	-0.01271993663372939
1104	42-43	-0.02089756018460065
1104	44-45	0.0181969449663697
1104	46-47	-0.2185814196855702
1104	48-49	-0.12033569615135065
1104	50-51	-0.0826089309314213
1104	52-53	-0.02588558498444371
1104	54-55	-0.1459119262828139
1104	56-57	-0.220265938938482
1104	58-59	-0.10449981079224102
1104	60-61	-0.11221426440662441
1104	62-63	-0.45100871699290934
1104	64-65	-0.3681567172912068
1104	66-67	-0.14625938352913437
1104	68-69	-0.21160661774215583
1104	70-71	-0.16669662704116206
1104	72-73	-0.19877996255256392
1104	74-75	-0.3227526548416151
1104	76-77	-0.309518250459341
1104	78-79	-0.20673481777971858
1104	80-81	-0.2372521986523779
1104	82-83	-0.17445480476031605
1104	84-85	-0.2888592815505078
1104	86-87	-0.1630542426670658
1104	88-89	-0.14470401362359553
1104	90-91	-0.09555801002141351
1104	92-93	-0.19549869237795292
1104	94-95	-0.08693589970366133
1104	96-97	-0.1345545264891541
1104	98-99	-0.09430651757283925
1104	100-101	-0.1826191088140945
1105	1	-0.10641557466978924
1105	2	-0.011076279687280532
1105	3	0.09252367993403254
1105	4	0.04244815136782876
1105	5	-0.12867928409713159
1105	6	-0.21572269832919488
1105	7	-0.13282635537889576
1105	8	0.02868969190696191
1105	9	0.037282702734820816
1105	10-11	-0.0606509418117227
1105	12-13	0.0644564633536504
1105	14-15	0.004412276641033941
1105	16-17	-0.03799942199758988
1105	18-19	0.0636225217384947
1105	20-21	-0.060933077121148926
1105	22-23	-0.07817199157912569
1105	24-25	-0.03231894822993553
1105	26-27	-0.1441907062655261
1105	28-29	-0.10343630934587367
1105	30-31	0.08463022429640432
1105	32-33	0.1685873379795595
1105	34-35	0.14665583236069324
1105	36-37	0.08866959685335019
1105	38-39	0.1773021794225187
1105	40-41	0.13071467601730546
1105	42-43	0.025022393617810224
1105	44-45	0.16636458888960703
1105	46-47	0.12218794776290309
1105	48-49	0.10575483334402946
1105	50-51	0.08676073857817101
1105	52-53	0.06754680662891843
1105	54-55	0.041330433347603446
1105	56-57	-0.1544565928119752
1105	58-59	-0.056050095085062424
1105	60-61	-0.011328070376769972
1105	62-63	0.1150715957291979
1105	64-65	-0.13220033847101575
1105	66-67	0.04157821419652663
1105	68-69	0.08290297714057004
1105	70-71	0.10042535234761374
1105	72-73	0.28195475670827364
1105	74-75	0.09955100542846651
1105	76-77	-0.08494913176708252
1105	78-79	0.09988165343563082
1105	80-81	0.08113577220760959
1105	82-83	0.10000770412383275
1105	84-85	0.10317163529384032
1105	86-87	0.03529330530734853
1105	88-89	0.09245616761307218
1105	90-91	0.09900933184070482
1105	92-93	0.3131833438693832
1105	94-95	0.041537803210339064
1105	96-97	-0.0898517013292377
1105	98-99	-0.046209946855000794
1105	100-101	0.05578263247944193
1106	1	0.041149686374389205
1106	2	0.09326608979063167
1106	3	0.05509898113884759
1106	4	0.03225738831562097
1106	5	0.07982975204744491
1106	6	-0.2029465939115198
1106	7	-0.013072339314632586
1106	8	-0.01807235628580628
1106	9	0.07317627703201168
1106	10-11	0.06605588549751218
1106	12-13	0.07434602158658521
1106	14-15	-0.004799570748524218
1106	16-17	0.06128521655663377
1106	18-19	-0.012293642920141679
1106	20-21	0.06642636062986185
1106	22-23	0.004107124886736813
1106	24-25	-0.1281271811616591
1106	26-27	0.05589714514010069
1106	28-29	0.0900501364372559
1106	30-31	0.08399016405544302
1106	32-33	-0.15066467005257067
1106	34-35	0.1325117560554787
1106	36-37	0.1975676892228293
1106	38-39	0.0850707537197124
1106	40-41	0.10285323023416737
1106	42-43	0.11632510446118971
1106	44-45	0.14908296238358076
1106	46-47	-0.22113534540978463
1106	48-49	0.2251699939866043
1106	50-51	0.19470501568660836
1106	52-53	0.05764469819518325
1106	54-55	0.14012561407049162
1106	56-57	0.26258657987879275
1106	58-59	0.26952721415188563
1106	60-61	0.4479967288200086
1106	62-63	0.2884651700263845
1106	64-65	0.3469788783964489
1106	66-67	0.4461464872888925
1106	68-69	0.23400739481125044
1106	70-71	0.14305838447612018
1106	72-73	0.34116660409782895
1106	74-75	0.3193552222959326
1106	76-77	0.21311813730921614
1106	78-79	0.34740926387740245
1106	80-81	0.4634525392758846
1106	82-83	0.4466693507101809
1106	84-85	0.38786039031391084
1106	86-87	0.34543386755634486
1106	88-89	0.09028498287411679
1106	90-91	0.25746816718206844
1106	92-93	0.14045493021476574
1106	94-95	0.19480085541917447
1106	96-97	0.2493927765824111
1106	98-99	0.2089983864783349
1106	100-101	0.1621330340858691
1107	1	0.11238259488705182
1107	2	0.07354558929152688
1107	3	0.14284882444270153
1107	4	0.08452425926763851
1107	5	0.09382794133639294
1107	6	0.018610153308571853
1107	7	0.15018183344577096
1107	8	0.1491978807316272
1107	9	0.1780582325999447
1107	10-11	0.18077487707073914
1107	12-13	0.17637842867157616
1107	14-15	0.09813819186260986
1107	16-17	0.17708367048796703
1107	18-19	0.16333952559206466
1107	20-21	0.13287713482490915
1107	22-23	0.1046073918505428
1107	24-25	0.1236661192074493
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2316	52-53	-0.31481649327557903
2316	54-55	-0.2346485542359673
2316	56-57	-0.2865900694117798
2316	58-59	-0.4621329508634773
2316	60-61	-0.5863728411504141
2316	62-63	-0.5639876210807344
2316	64-65	-0.37156578737263857
2316	66-67	-0.21514337128581218
2316	68-69	-0.4002632119711791
2316	70-71	-0.2940021786934608
2316	72-73	-0.23155407805772654
2316	74-75	-0.16065076152472813
2316	76-77	-0.022120812760398678
2316	78-79	-0.2516315987133666
2316	80-81	-0.24679820582486656
2316	82-83	-0.12627572472047888
2316	84-85	-0.20015931026490108
2316	86-87	-0.20260067750067634
2316	88-89	-0.21944390402016012
2316	90-91	-0.2455525115212822
2316	92-93	-0.11359853292145061
2316	94-95	-0.28512170013254234
2316	96-97	-0.29354976723180926
2316	98-99	-0.7100560547824131
2316	100-101	-0.885651226164299
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	14.0
15	25.0
16	65.0
17	168.0
18	333.0
19	618.0
20	971.0
21	1607.0
22	2521.0
23	3043.0
24	2853.0
25	2258.0
26	1920.0
27	1670.0
28	1820.0
29	2252.0
30	2551.0
31	3197.0
32	4198.0
33	5720.0
34	8532.0
35	15113.0
36	33793.0
37	99562.0
38	205891.0
39	89456.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22821751254425	18.77880889876007	13.677617960830105	23.315355627865582
2	15.039745174091612	20.14258504733122	41.42776737755264	23.389902401024525
3	18.406096484162315	25.815437384401108	30.32886673831164	25.449599393124938
4	11.846299102126713	17.560835350472388	39.81292097369188	30.779944573709013
5	12.723628050956506	38.34850187304106	35.605113595810586	13.322756480191853
6	30.01951543799617	38.35589062382107	18.318001717032267	13.306592221150492
7	26.586266596789027	33.239052899684125	22.298376160577185	17.876304342949663
8	25.178177784212686	36.32094228773138	20.034830060707897	18.46604986734804
9	25.879877075172164	16.263476766024784	20.247317901794727	37.60932825700833
10-11	24.18170769259005	26.54588982852109	29.011421731266097	20.260980747622767
12-13	25.550235429186696	24.04609884966995	28.73123143025525	21.672434290888106
14-15	22.35690208225849	25.40759124028052	26.816597706681133	25.418908970779864
16-17	21.67518725236092	28.758761044983387	27.232702735220165	22.333348967435523
18-19	21.56470277540141	27.519085409437732	29.530662582604872	21.38554923255598
20-21	22.455769695498915	26.580702525392084	29.714729703411475	21.248798075697525
22-23	22.100570780678698	26.678949633040432	29.271321686606942	21.949157899673928
24-25	21.73860732777167	26.789577899633144	29.559770872036236	21.912043900558952
26-27	21.877220906318414	27.003059943695405	29.303166904073162	21.816552245913023
28-29	21.811713749105287	26.977594972072733	29.430993452030158	21.77969782679182
30-31	22.1655202791299	26.738189285473947	29.349220300133975	21.747070135262174
32-33	21.726575866520115	26.930182857818718	29.477385950923875	21.865855324737296
34-35	22.112704203466286	26.921822011864244	29.387149991537193	21.578323793132277
36-37	22.14372092738704	26.867937687487125	29.18803671535809	21.800304669767744
38-39	21.94790694828931	27.200750449913073	29.135606095366278	21.715736506431334
40-41	22.18948124009699	26.922637704152486	29.181799457972474	21.706081597778056
42-43	22.10179431911106	26.86380589786309	29.099924344540266	21.934475438485585
44-45	22.141559318162816	27.050191585726203	28.992762770172583	21.8154863259384
46-47	22.257871076699658	27.13237033834004	28.499661415202866	22.11009716975744
48-49	22.268241378290718	27.177948327353224	28.524875325133493	22.028934969222565
50-51	22.236791392814975	27.533489266509104	28.242427826526722	21.987291514149202
52-53	22.334266621259797	27.47180253721086	28.174521443530644	22.0194093979987
54-55	22.249434623282713	27.44417096094669	28.263533864484963	22.042860551285635
56-57	22.208038239654474	27.644117533101813	28.15147813638783	21.996366090855883
58-59	22.343545121038538	27.52584215130684	28.216937442519185	21.913675285135437
60-61	22.419388881921268	27.503678393681426	28.243537702133214	21.833395022264096
62-63	22.18526366452597	27.465386192001862	28.342840361464212	22.006509782007956
64-65	22.000050985290745	27.453743594972853	28.398195120707676	22.14801029902873
66-67	22.244235594977958	27.54658071594558	28.113614834860616	22.095568854215845
68-69	22.234854471391355	27.45950741025863	28.174175490830855	22.13146262751916
70-71	22.291194029089834	27.29611370831655	28.170847969166612	22.241844293427
72-73	22.33978663028594	27.426970886239864	28.038652485523674	22.194589997950526
74-75	22.207811276429133	27.502304391803705	27.93606026494388	22.353824066823282
76-77	22.24044000012236	27.490218990605946	27.984342227755533	22.284998781516162
78-79	22.339058813453214	27.438868961073126	27.906770449915474	22.31530177555819
80-81	22.22771681610829	27.61087807235598	27.78869899119256	22.37270612034316
82-83	22.387694466139592	27.686839416698444	27.734353492488495	22.19111262467347
84-85	22.442243887915723	27.61495653379719	27.723851454277387	22.2189481240097
86-87	22.233313416765398	27.690928852989217	27.8145067386654	22.26125099157998
88-89	22.303340025552107	27.61972834825912	27.87616710222169	22.20076452396708
90-91	22.355407461183006	27.63776107411018	27.789990578696433	22.216840886010385
92-93	22.35812562069085	27.70284737785518	27.884440873524873	22.0545861279291
94-95	22.365455876624633	27.828796694545225	27.69909104072472	22.10665638810542
96-97	22.238728661999545	27.694282608828647	27.849570028202557	22.217418700969247
98-99	22.26911319973653	27.610266303139802	27.767694914770352	22.352925582353315
100-101	22.306329160387538	27.73598487706498	27.761271338000455	22.196414624547035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	13.5
2	26.0
3	47.5
4	43.5
5	37.5
6	67.0
7	173.5
8	352.5
9	447.0
10	394.0
11	354.5
12	369.0
13	392.5
14	446.0
15	473.0
16	490.0
17	529.0
18	558.0
19	633.0
20	743.5
21	884.0
22	1096.0
23	1381.5
24	1716.0
25	2183.0
26	2839.5
27	3631.0
28	4468.0
29	5470.5
30	6757.0
31	7889.0
32	8957.5
33	10122.5
34	11151.0
35	12123.0
36	13326.0
37	14239.0
38	14655.5
39	15078.5
40	15492.0
41	15995.5
42	16531.0
43	16999.0
44	17552.5
45	18131.0
46	18297.5
47	18353.5
48	18502.5
49	18199.5
50	17651.5
51	16969.0
52	16235.0
53	15756.0
54	15176.0
55	14337.5
56	13243.5
57	11828.0
58	10463.0
59	8986.0
60	7290.0
61	5887.5
62	4762.0
63	3604.5
64	2620.5
65	1924.0
66	1383.0
67	1044.0
68	764.5
69	528.0
70	380.5
71	295.5
72	218.5
73	152.0
74	98.5
75	60.0
76	40.0
77	24.0
78	18.5
79	9.0
80	4.0
81	3.5
82	1.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10502038211105241
2	0.001427461504422072
3	0.0
4	0.0
5	4.07846144120592E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.003976499905175772
20-21	0.004384346049296363
22-23	0.0
24-25	0.0
26-27	0.0025490384007536998
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.002854923008844144
38-39	0.0017333461125125156
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.021411922566331076
48-49	0.0011215768963316278
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0035686537610551796
62-63	0.00856476902653243
64-65	0.008870653634622874
66-67	0.004588269121356659
68-69	0.002752961472813996
70-71	7.13730752211036E-4
72-73	0.0023451153286934037
74-75	0.0026509999367838478
76-77	0.0035686537610551796
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.07846144120592E-4
88-89	0.0021411922566331077
90-91	6.117692161808879E-4
92-93	0.0
94-95	0.008156922882411839
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	490381.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.07312449050154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.38270753698717	67.5147746240827
2	10.349397653842841	16.367184181680393
3	2.3945053215178556	5.680230521446496
4	0.8376341331862639	2.649373923637232
5	0.38671877941900973	1.5289531093907083
6	0.2022339438737196	0.9594761892079041
7	0.11305846384039431	0.6257920190169456
8	0.07534612184435661	0.4766282617980247
9	0.05357061420161404	0.3812396261217172
>10	0.19681428263422124	2.4209427467134357
>50	0.00594523993003038	0.3388870170288705
>100	0.0012924429515688927	0.1718961990473215
>500	2.584885903136223E-4	0.17210059405206868
>1k	5.169771806272446E-4	0.7125209867761417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1871	0.38154006782481376	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1615	0.32933576137737797	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.17170322667476923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.0196153603014798E-4	2.0392307206029597E-4
22-23	0.0	0.0	0.0	3.0588460809044397E-4	2.0392307206029597E-4
24-25	0.0	0.0	0.0	6.117692161808879E-4	2.0392307206029597E-4
26-27	0.0	0.0	0.0	8.156922882411839E-4	2.0392307206029597E-4
28-29	0.0	0.0	0.0	8.156922882411839E-4	2.0392307206029597E-4
30-31	0.0	0.0	0.0	8.156922882411839E-4	2.0392307206029597E-4
32-33	0.0	0.0	0.0	0.0013254999683919239	2.0392307206029597E-4
34-35	0.0	0.0	0.0	0.0019372691845728116	2.0392307206029597E-4
36-37	0.0	0.0	0.0	0.0034666922250250315	2.0392307206029597E-4
38-39	0.0	0.0	0.0	0.005403961409597843	2.0392307206029597E-4
40-41	0.0	0.0	0.0	0.006627499841959619	2.0392307206029597E-4
42-43	0.0	0.0	0.0	0.008462807490502284	2.0392307206029597E-4
44-45	0.0	0.0	0.0	0.009992230530954502	2.0392307206029597E-4
46-47	0.0	0.0	0.0	0.011725576643467019	2.0392307206029597E-4
48-49	0.0	0.0	0.0	0.015905999620703087	2.0392307206029597E-4
50-51	0.0	0.0	0.0	0.01865896109351708	2.0392307206029597E-4
52-53	0.0	0.0	0.0	0.021513884102361224	2.0392307206029597E-4
54-55	0.0	0.0	0.0	0.024572730183265664	2.0392307206029597E-4
56-57	0.0	0.0	0.0	0.029160999304622324	2.0392307206029597E-4
58-59	0.0	2.0392307206029597E-4	0.0	0.03344338381788854	2.0392307206029597E-4
60-61	0.0	4.0784614412059194E-4	0.0	0.037012037578943724	2.0392307206029597E-4
62-63	0.0	6.117692161808879E-4	0.0	0.04190619130839082	4.0784614412059194E-4
64-65	0.0	6.117692161808879E-4	0.0	0.04669838350180778	4.0784614412059194E-4
66-67	0.0	6.117692161808879E-4	0.0	0.052306267983465916	4.0784614412059194E-4
68-69	0.0	6.117692161808879E-4	0.0	0.059443575505576274	4.0784614412059194E-4
70-71	0.0	6.117692161808879E-4	0.0	0.06627499841959619	4.0784614412059194E-4
72-73	0.0	6.117692161808879E-4	0.0	0.07331034440567641	4.0784614412059194E-4
74-75	0.0	6.117692161808879E-4	0.0	0.0788162673513044	4.0784614412059194E-4
76-77	0.0	6.117692161808879E-4	0.0	0.08625945948150521	4.0784614412059194E-4
78-79	0.0	6.117692161808879E-4	0.0	0.09502815158009792	4.0784614412059194E-4
80-81	0.0	6.117692161808879E-4	0.0	0.10308311292647962	4.0784614412059194E-4
82-83	0.0	6.117692161808879E-4	0.0	0.11399299728170545	4.0784614412059194E-4
84-85	0.0	6.117692161808879E-4	0.0	0.12510680470899158	4.0784614412059194E-4
86-87	0.0	8.156922882411839E-4	0.0	0.13876965053703139	4.0784614412059194E-4
88-89	0.0	0.0010196153603014798	0.0	0.15029130410843813	4.0784614412059194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	860	0.0	62.45181	1
GTATCAA	1550	0.0	45.99648	1
ATCAACG	1875	0.0	37.743305	3
CAACGCA	1880	0.0	37.64292	5
TCAACGC	1890	0.0	37.443752	4
TATCAAC	1985	0.0	35.891006	2
AACGCAG	2000	0.0	35.859303	6
ACTGCGT	55	0.0025793156	34.542377	8
ACGCAGA	2335	0.0	30.307793	7
CGCAGAG	2405	0.0	29.425652	8
ATAAACG	65	0.0058433907	29.228167	3
GTGGTAT	310	0.0	29.131104	1
GCAGAGT	2630	0.0	26.547064	9
TACATGG	1525	0.0	25.22726	2
TGGTATC	360	0.0	25.067211	2
GTACATG	1615	0.0	24.427027	1
GAGTACT	1730	0.0	23.749094	12-13
ACATGGG	1620	0.0	23.747885	3
CAGAGTA	2575	0.0	23.425097	10-11
GTACTTT	1835	0.0	22.520725	14-15
>>END_MODULE