FastQCFastQC Report
Fri 10 Feb 2017
SRR2031471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577029
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14160.24539494548800841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.1597839969914857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.1010347833471108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.061.0778851
GTATCAA13100.049.275751
CAACGCA16550.038.7236985
TCAACGC16600.038.321084
ATCAACG17000.037.4194073
AACGCAG17850.036.1600156
TATCAAC18500.034.3854032
ACGCAGA19450.032.4533847
AACCGTC600.003965642331.6400137
CGCAGAG19750.031.4798098
GTACATG22500.029.5332431
TACATGG22350.028.8872
ACATGGG22200.028.4406623
GCAGAGT24000.025.7052769
ATACGTA500.001654646923.74753424-25
CATGGGA10150.023.385414
TATAGGC1452.3422317E-522.9177043
GAGTACT12550.022.12087812-13
CATGGGG14500.021.6081184
AGAGTAC21350.021.5607610-11
Produced by FastQC (version 0.11.5)