##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031471_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577029
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78648560124361	34.0	33.0	34.0	31.0	34.0
2	32.86867904386088	34.0	33.0	34.0	31.0	34.0
3	33.04575853206685	34.0	34.0	34.0	31.0	34.0
4	36.00438799436424	37.0	37.0	37.0	35.0	37.0
5	35.996650081711664	37.0	37.0	37.0	35.0	37.0
6	36.08839763686054	37.0	37.0	37.0	35.0	37.0
7	36.126778723426376	37.0	37.0	37.0	35.0	37.0
8	36.20743151557374	37.0	37.0	37.0	35.0	37.0
9	38.138284211018856	39.0	39.0	39.0	37.0	39.0
10-11	38.222763500621284	39.0	39.0	39.0	37.0	39.0
12-13	38.26580379842261	39.0	39.0	39.0	37.0	39.0
14-15	39.86945803417159	41.0	40.0	41.0	38.0	41.0
16-17	39.87922877359716	41.0	40.0	41.0	38.0	41.0
18-19	39.892681303712635	41.0	40.0	41.0	38.0	41.0
20-21	39.82610839316568	41.0	40.0	41.0	38.0	41.0
22-23	39.75653823291377	41.0	40.0	41.0	38.0	41.0
24-25	39.642601151761866	41.0	40.0	41.0	38.0	41.0
26-27	39.54584864885474	41.0	40.0	41.0	37.5	41.0
28-29	39.471828972200704	41.0	40.0	41.0	37.0	41.0
30-31	39.31559938928546	41.0	40.0	41.0	37.0	41.0
32-33	39.134202093828904	41.0	39.5	41.0	36.0	41.0
34-35	39.045209166263746	41.0	39.0	41.0	35.0	41.0
36-37	38.922426775777296	41.0	39.0	41.0	35.0	41.0
38-39	38.738427358070396	41.0	39.0	41.0	35.0	41.0
40-41	38.604781562105195	40.0	39.0	41.0	35.0	41.0
42-43	38.81352756967154	41.0	39.0	41.0	35.0	41.0
44-45	38.796893223737456	41.0	39.0	41.0	35.0	41.0
46-47	38.6289224631691	41.0	39.0	41.0	35.0	41.0
48-49	38.42867082936906	41.0	39.0	41.0	35.0	41.0
50-51	37.399978164009084	40.0	38.0	40.5	33.0	40.5
52-53	37.45069571893267	39.5	38.0	40.5	33.5	40.5
54-55	37.88446334586303	40.0	38.0	41.0	34.0	41.0
56-57	37.89146905961398	40.0	38.0	41.0	34.0	41.0
58-59	37.90070083132737	40.0	37.0	41.0	34.0	41.0
60-61	37.69486282318566	40.0	37.0	41.0	34.0	41.0
62-63	37.43732810655963	40.0	36.0	41.0	33.5	41.0
64-65	37.15813156704429	39.0	36.0	41.0	33.0	41.0
66-67	36.89588044968278	39.0	35.0	41.0	33.0	41.0
68-69	36.57066109328994	38.5	35.0	41.0	33.0	41.0
70-71	36.20548793908105	37.0	35.0	40.5	32.0	41.0
72-73	35.835652974113955	37.0	35.0	39.0	32.0	41.0
74-75	35.457957918926084	36.0	35.0	39.0	32.0	41.0
76-77	35.097021120255654	36.0	35.0	39.0	31.5	40.5
78-79	34.71699949222656	35.5	35.0	37.0	31.0	39.0
80-81	34.33856097353859	35.0	35.0	37.0	31.0	39.0
82-83	34.04438425105151	35.0	35.0	36.5	31.0	39.0
84-85	33.75975384252784	35.0	35.0	36.0	30.0	37.0
86-87	33.52639035473087	35.0	35.0	36.0	30.0	37.0
88-89	33.353238745366355	35.0	34.5	35.5	30.0	36.5
90-91	33.15772864102151	35.0	34.0	35.0	29.0	36.0
92-93	33.08303482147345	35.0	34.0	35.0	29.0	36.0
94-95	32.98373305327809	35.0	34.0	35.0	29.0	36.0
96-97	32.8965693232056	35.0	34.0	35.0	29.0	36.0
98-99	32.82108351573318	35.0	34.0	35.0	29.0	35.5
100-101	32.45674134228956	35.0	33.5	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10909198087136929
1101	2	0.09972949431036682
1101	3	0.1368536863652352
1101	4	0.15213121944884023
1101	5	-0.0028538615700526293
1101	6	0.08948772015606465
1101	7	0.06852781189148516
1101	8	0.032047580711996204
1101	9	0.12197884070975107
1101	10-11	0.15764384629139272
1101	12-13	0.15592568802690465
1101	14-15	0.13973248979757358
1101	16-17	0.1712678371884877
1101	18-19	0.24805447759896282
1101	20-21	0.18016478542291026
1101	22-23	0.2285351589016429
1101	24-25	0.30380959571888866
1101	26-27	0.1738438353404561
1101	28-29	0.1988722452392082
1101	30-31	0.28245908072313597
1101	32-33	0.34231976548267085
1101	34-35	0.2769148009825315
1101	36-37	0.362549301394246
1101	38-39	0.2716605232214775
1101	40-41	0.3838942711629514
1101	42-43	0.27601117967345346
1101	44-45	0.31421721075448517
1101	46-47	0.5229312412973073
1101	48-49	0.5819352787132104
1101	50-51	0.3426702079402446
1101	52-53	0.2437774738024885
1101	54-55	0.327330877411697
1101	56-57	0.4601058536500986
1101	58-59	0.4867561609536395
1101	60-61	0.48242616573440245
1101	62-63	0.49101988088224857
1101	64-65	0.6054188752033483
1101	66-67	0.5554381308476977
1101	68-69	0.5360442928787634
1101	70-71	0.4344917851569292
1101	72-73	0.418257716935031
1101	74-75	0.3960486533063303
1101	76-77	0.44179833811285363
1101	78-79	0.4055664014616198
1101	80-81	0.46511818826065365
1101	82-83	0.424854415288884
1101	84-85	0.47981344329669895
1101	86-87	0.5375195269433632
1101	88-89	0.3085568491046544
1101	90-91	0.2769830238814919
1101	92-93	0.32005236799910364
1101	94-95	0.4082119976633791
1101	96-97	0.528395523067239
1101	98-99	0.505181897161485
1101	100-101	0.4500270773599695
1102	1	0.07502683966336576
1102	2	0.10369774827861988
1102	3	0.1006755787585547
1102	4	0.008810254699298525
1102	5	0.17141433055527955
1102	6	0.052742719125348
1102	7	0.036189118838507284
1102	8	0.18905129128713583
1102	9	0.13599656901319435
1102	10-11	0.16264281557607063
1102	12-13	0.149174502704291
1102	14-15	0.09583690125913336
1102	16-17	0.1476902243251672
1102	18-19	0.23009426321016946
1102	20-21	0.255381235639355
1102	22-23	0.20895156788948555
1102	24-25	0.22410953387596777
1102	26-27	0.1253100278780792
1102	28-29	0.19114188036598279
1102	30-31	0.33237146992123456
1102	32-33	0.3958267743468227
1102	34-35	0.32057847857477384
1102	36-37	0.22101920450700874
1102	38-39	0.34200684356779476
1102	40-41	0.22560216644227182
1102	42-43	0.3435101489581385
1102	44-45	0.2163894432838518
1102	46-47	0.4834677286195088
1102	48-49	0.19281447887812675
1102	50-51	0.1489859160416671
1102	52-53	0.1102869563833977
1102	54-55	0.16120533628615874
1102	56-57	0.19923180706177135
1102	58-59	0.2909975544807466
1102	60-61	0.3059805875031074
1102	62-63	0.19709852446089116
1102	64-65	0.09491846291721373
1102	66-67	0.05975425123524758
1102	68-69	0.3307386857874377
1102	70-71	0.2603008966803273
1102	72-73	0.09716412798429985
1102	74-75	0.010303446132546412
1102	76-77	0.12128452652761013
1102	78-79	0.10664607575558449
1102	80-81	0.09145811817200666
1102	82-83	0.22829700444575707
1102	84-85	0.11094619942945627
1102	86-87	0.292840594764435
1102	88-89	0.24757715419638515
1102	90-91	0.2803715004842502
1102	92-93	0.10586972524502158
1102	94-95	0.1415659453030358
1102	96-97	0.2709486049060317
1102	98-99	-0.14567617333944582
1102	100-101	0.09305171145603452
1103	1	-0.09551119373180939
1103	2	0.005515930096798627
1103	3	-0.10288657337502372
1103	4	-0.05007657275895383
1103	5	-0.05756526128145367
1103	6	-0.06495672428837906
1103	7	-0.11286468450101239
1103	8	-0.07580956214515311
1103	9	-0.058771520040615144
1103	10-11	-0.08199540334785382
1103	12-13	0.00702236912358245
1103	14-15	0.07633782890291485
1103	16-17	-0.05065856973791227
1103	18-19	-0.04216918762470101
1103	20-21	0.06213448239260089
1103	22-23	-0.012792402425915839
1103	24-25	0.01720786411716091
1103	26-27	-0.06020017620355844
1103	28-29	-0.003422127055166868
1103	30-31	0.07903195229600612
1103	32-33	0.014379650042549486
1103	34-35	0.01894582551354773
1103	36-37	-0.11987133352638324
1103	38-39	-0.11422692266597068
1103	40-41	-0.04834959608091083
1103	42-43	0.017703098865375466
1103	44-45	0.22052673456400385
1103	46-47	0.07493340579947727
1103	48-49	-0.04039516861724479
1103	50-51	0.06720483997487747
1103	52-53	0.20023129775631077
1103	54-55	0.24328325836407316
1103	56-57	0.15212605567030124
1103	58-59	0.17570998490746348
1103	60-61	0.12789153369976702
1103	62-63	0.2054102127725841
1103	64-65	0.14267356495803796
1103	66-67	0.04320508611465357
1103	68-69	0.014337943672408926
1103	70-71	-0.17441153374638674
1103	72-73	-0.33125887758156125
1103	74-75	-0.3029989657412884
1103	76-77	-0.31268578887127063
1103	78-79	-0.17274167934645845
1103	80-81	-0.13457156642910206
1103	82-83	-0.23199984156537568
1103	84-85	-0.1912615927783392
1103	86-87	-0.06845810653426554
1103	88-89	-0.17728369923589327
1103	90-91	-0.024730540332079443
1103	92-93	-0.1453733174265821
1103	94-95	-0.10242292297154876
1103	96-97	-0.18563766596594888
1103	98-99	0.02001955949914702
1103	100-101	-0.17622472639182973
1104	1	0.29060391418185816
1104	2	0.3033380360484017
1104	3	0.3164528641658535
1104	4	0.26672525850330686
1104	5	0.2487591337479813
1104	6	0.265546872833923
1104	7	0.33644205192802445
1104	8	0.3001616608764337
1104	9	0.3809145492857482
1104	10-11	0.3649208818058369
1104	12-13	0.37986082563110557
1104	14-15	0.3960631395634806
1104	16-17	0.40863508624638456
1104	18-19	0.34304990982460737
1104	20-21	0.3875988381807005
1104	22-23	0.46715854590632944
1104	24-25	0.4801542342937424
1104	26-27	0.40653625283503203
1104	28-29	0.4508420979284864
1104	30-31	0.515215732544533
1104	32-33	0.48803890445720555
1104	34-35	0.3677064533749004
1104	36-37	0.34498478158152324
1104	38-39	0.22112951945306492
1104	40-41	0.1969152829249765
1104	42-43	0.41654874961749755
1104	44-45	0.35826054751364467
1104	46-47	0.3025004430787348
1104	48-49	0.46218821921794273
1104	50-51	0.2991407457956754
1104	52-53	0.3511273082206614
1104	54-55	0.37914656771658883
1104	56-57	0.36797955468927057
1104	58-59	0.242936930623614
1104	60-61	0.2561714552170997
1104	62-63	0.30594651100671655
1104	64-65	0.4006755270247453
1104	66-67	0.2699022738190351
1104	68-69	0.16840269187384393
1104	70-71	0.03432620333666847
1104	72-73	0.1692283689848182
1104	74-75	0.10613772490619766
1104	76-77	0.08684658522944488
1104	78-79	0.25203922320422834
1104	80-81	0.25163183448652404
1104	82-83	0.295043977924486
1104	84-85	0.18646240983775186
1104	86-87	0.10176099091504653
1104	88-89	0.04107797506102884
1104	90-91	0.11683257281377024
1104	92-93	0.17354100566039676
1104	94-95	0.24567060220831394
1104	96-97	0.14274689910926242
1104	98-99	0.11213205132386861
1104	100-101	0.28922000872915987
1105	1	-0.010973671916801209
1105	2	-0.13539474735837587
1105	3	-0.03554721116655202
1105	4	-0.23237389149606003
1105	5	-0.11827603392939778
1105	6	0.013716923181078755
1105	7	-0.03731653282720515
1105	8	0.08181696490546386
1105	9	0.15231209093828824
1105	10-11	0.2000480680149863
1105	12-13	0.1910997925727358
1105	14-15	0.15578729740997943
1105	16-17	0.19947152704138915
1105	18-19	0.15785461056663053
1105	20-21	0.25891696692377764
1105	22-23	0.20306353918918774
1105	24-25	0.24369366452965835
1105	26-27	0.33250647142455847
1105	28-29	0.2509789517961778
1105	30-31	0.4734235118708128
1105	32-33	0.22835712108808792
1105	34-35	0.23053713124098607
1105	36-37	0.3874236053915894
1105	38-39	0.28456254266799874
1105	40-41	0.25631802251340474
1105	42-43	0.37443322737561147
1105	44-45	0.4864778689444549
1105	46-47	0.4542607102576639
1105	48-49	0.5750895212129947
1105	50-51	0.481398081288539
1105	52-53	0.43195270026304655
1105	54-55	0.41487139099932335
1105	56-57	0.5171157431207192
1105	58-59	0.5057559947440424
1105	60-61	0.5234539642883789
1105	62-63	0.4633096257505258
1105	64-65	0.5085342662114058
1105	66-67	0.3576331362495111
1105	68-69	0.4659609803216185
1105	70-71	0.24060829807972794
1105	72-73	0.2684301593849625
1105	74-75	0.22857171488698214
1105	76-77	0.3860402146191291
1105	78-79	0.3006725678374167
1105	80-81	0.4511669707754038
1105	82-83	0.5899420908477637
1105	84-85	0.4616862098553298
1105	86-87	0.5328396881666677
1105	88-89	0.5649818885794318
1105	90-91	0.589524576279068
1105	92-93	0.5155563985816585
1105	94-95	0.552149502857425
1105	96-97	0.6198835144243446
1105	98-99	0.667033679759335
1105	100-101	0.5540275344363437
1106	1	0.15527918427506648
1106	2	0.0057596039487251005
1106	3	0.12875353908982845
1106	4	0.11445326166779068
1106	5	0.0707633860162673
1106	6	-0.15822132909937636
1106	7	0.1242674617032975
1106	8	0.10439662342186296
1106	9	0.07550526846298311
1106	10-11	0.05131918584302042
1106	12-13	-0.13572052372240506
1106	14-15	-0.19440881277156308
1106	16-17	0.024299255941564013
1106	18-19	-0.023893648730613393
1106	20-21	0.044855507072391276
1106	22-23	0.08207795058876854
1106	24-25	0.026870469552960685
1106	26-27	-0.19846634302643196
1106	28-29	0.03932388981456114
1106	30-31	0.0013062415806004424
1106	32-33	-0.11381466423423348
1106	34-35	0.06681211729736702
1106	36-37	0.23944138125024494
1106	38-39	0.042892727443373246
1106	40-41	-0.06617527555607694
1106	42-43	0.16898082457608865
1106	44-45	0.23386006789733926
1106	46-47	-0.23409502281658234
1106	48-49	0.060682619573952934
1106	50-51	0.01354036012482851
1106	52-53	0.11711039803746814
1106	54-55	0.2705684817317717
1106	56-57	0.029979851359151155
1106	58-59	0.2963004253948114
1106	60-61	-0.10167422700001083
1106	62-63	0.05699721683381398
1106	64-65	0.08779696389586888
1106	66-67	0.14068399895439399
1106	68-69	0.1681523766620998
1106	70-71	0.03459815072721284
1106	72-73	0.13227751729504433
1106	74-75	0.07854742601129061
1106	76-77	0.16769221800158363
1106	78-79	0.2530034003161461
1106	80-81	0.30481300089360985
1106	82-83	0.1941638572787312
1106	84-85	0.3292038867593092
1106	86-87	-0.019907653550824023
1106	88-89	0.34364725983939337
1106	90-91	0.5135543706332513
1106	92-93	0.3609622027233641
1106	94-95	0.5185049120799974
1106	96-97	-0.025953192363949995
1106	98-99	0.4672891627481306
1106	100-101	0.40687742918454717
1107	1	0.20648542217343646
1107	2	0.18830011714494788
1107	3	0.23559673688493632
1107	4	0.24259532387233662
1107	5	0.18128722295174526
1107	6	0.16069471395189083
1107	7	0.17433113152728907
1107	8	0.21258299284639293
1107	9	0.25146075191754846
1107	10-11	0.22522502427866442
1107	12-13	0.21238769753460218
1107	14-15	0.30277837651097883
1107	16-17	0.275781977870146
1107	18-19	0.23221167685768052
1107	20-21	0.2924744300929589
1107	22-23	0.19151155081204507
1107	24-25	0.1635207390594715
1107	26-27	0.23681720573767961
1107	28-29	0.21905595324017924
1107	30-31	0.26607240761057227
1107	32-33	0.3293927249541042
1107	34-35	0.1601887112234408
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2316	68-69	-0.5469466910882943
2316	70-71	-1.1938917260981228
2316	72-73	-0.788836161758347
2316	74-75	-0.5888679833735324
2316	76-77	-0.6094616075110721
2316	78-79	-0.5469070102372271
2316	80-81	-0.6429892444689429
2316	82-83	-0.5800534252612763
2316	84-85	-0.5813646383473738
2316	86-87	-0.5215219947870722
2316	88-89	-0.6947441984344351
2316	90-91	-0.5457174878933273
2316	92-93	-0.5126712336161958
2316	94-95	-0.6081408071025294
2316	96-97	-0.6254018061079236
2316	98-99	-0.6543404130220338
2316	100-101	-0.550470203836305
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	205.0
3	58.0
4	67.0
5	72.0
6	102.0
7	129.0
8	217.0
9	205.0
10	211.0
11	202.0
12	305.0
13	385.0
14	521.0
15	553.0
16	693.0
17	884.0
18	1176.0
19	1499.0
20	2011.0
21	2478.0
22	3170.0
23	3467.0
24	3244.0
25	2688.0
26	2647.0
27	2738.0
28	3002.0
29	3303.0
30	4041.0
31	4932.0
32	6477.0
33	8550.0
34	12975.0
35	22602.0
36	49918.0
37	126948.0
38	218110.0
39	86104.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.546141589759685	19.265437888688737	13.662598672975266	23.525821848576317
2	14.82786545534629	21.140997206379375	40.73293090861167	23.298206429662667
3	17.526007351411334	27.139017962410712	30.906269505513556	24.4287051806644
4	12.10750823651812	17.770244494537888	39.7157967747529	30.40645049419109
5	12.716843217215281	38.61227279271341	35.535141567116675	13.135742422954635
6	29.30732184448256	38.411978835171354	19.191768539602545	13.088930780743544
7	25.925495468146924	33.30604102519624	23.609176460384234	17.159287046272606
8	25.446148187474193	34.844667908907	21.64308838991079	18.06609551370802
9	26.891980984766995	15.34317637669593	20.680453867240363	37.08438877129671
10-11	24.012267427846055	26.176668996217806	29.992248317434	19.81881525850214
12-13	25.180104343483904	24.1877315782082	29.287090224690466	21.345073853617432
14-15	22.408168611181704	25.351352804794168	27.10597007028357	25.134508513740556
16-17	21.714829174238115	28.400649873077082	27.531175874935844	22.35334507774896
18-19	21.787867835384482	27.60536791989204	29.263882857794155	21.342881386929324
20-21	23.042797630501827	26.79250941783642	28.930310610906833	21.234382340754916
22-23	22.61535579744833	26.807518332743633	28.53976314858368	22.03736272122436
24-25	22.256180922231877	26.90301574667138	28.76673208611062	22.074071244986122
26-27	22.123295400293394	27.145039625704193	28.65541695962779	22.076248014374624
28-29	22.20913958575379	27.162619099473062	28.65215969069061	21.976081624082543
30-31	22.535167264178206	26.987387603175968	28.546732171635515	21.93071296101031
32-33	22.324651317063342	27.10362414918585	28.507472924595223	22.06425160915558
34-35	22.4897593901553	27.090069624862522	28.46182090586104	21.958350079121136
36-37	22.662699191169704	26.918563167406518	28.309754746821707	22.108982894602068
38-39	22.43784511796207	27.302298803663426	28.242469818052985	22.017386260321516
40-41	22.442449583179293	27.138733691228108	28.386071610196705	22.032745115395898
42-43	22.499143594302193	26.816989354551957	28.488866725928496	22.195000325217354
44-45	22.51512925880289	27.270559446562654	28.04395310905293	22.170358185581527
46-47	22.571293286758408	27.129953066625966	27.95744429913869	22.34130934747694
48-49	22.58360901511977	27.270329525410876	27.863511704437894	22.282549755031464
50-51	22.658489688539042	27.386629460722045	27.599643905791215	22.355236944947695
52-53	22.724254856030974	27.401892161874354	27.520271613145674	22.353581368948998
54-55	22.734216969390616	27.225092693196594	27.698785543248572	22.341904794164215
56-57	22.74508952794357	27.41912099837222	27.516440586001085	22.319348887683123
58-59	22.71786932873303	27.259482049448625	27.717313960228672	22.305334661589672
60-61	22.657757155302928	27.421155080566916	27.596351535987367	22.32473622814279
62-63	22.62748361340669	27.402310192608702	27.68756634116427	22.282639852820335
64-65	22.591799411823477	27.23066163342789	27.776093274197123	22.40144568055151
66-67	22.67748087959918	27.42282855243771	27.49814739933046	22.40154316863265
68-69	22.638020573471415	27.370352855426027	27.55855977022964	22.43306680087292
70-71	22.717837822827956	27.048648292163964	27.5325105823182	22.70100330268988
72-73	22.68328266316503	27.287426131920643	27.5239254901178	22.505365714796525
74-75	22.572282354452376	27.51785089694953	27.367961513058255	22.54190523553984
76-77	22.610087208873452	27.305862841938367	27.483335372748723	22.600714576439458
78-79	22.68557464004966	27.303096202344832	27.34198863611701	22.669340521488504
80-81	22.70401779507855	27.515292645627692	27.191801056582786	22.58888850271097
82-83	22.763918883100068	27.292438851640544	27.34458170567966	22.59906055957973
84-85	22.878038739889682	27.137724363678682	27.29605019180614	22.688186704625494
86-87	22.612274889996403	27.363318469312777	27.313289198715218	22.7111174419756
88-89	22.717367608754373	27.3792556577808	27.2875395504	22.61583718306483
90-91	22.8240196184813	27.214826710649103	27.342694068881702	22.61845960198789
92-93	22.768429841874934	27.3477900854594	27.288768763138755	22.59501130952691
94-95	22.729608042573428	27.243835635459806	27.395710025792614	22.630846296174152
96-97	22.79106211760582	27.160909476650907	27.41541230238719	22.63261610335609
98-99	22.706760000624847	27.243685985500026	27.267378184778323	22.782175829096797
100-101	22.7398161627157	27.17906383436762	27.363873343420163	22.71724665949651
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	107.5
2	82.5
3	88.5
4	86.0
5	84.5
6	103.5
7	181.5
8	295.0
9	356.0
10	327.0
11	290.5
12	303.0
13	325.0
14	337.0
15	388.0
16	438.0
17	471.5
18	522.0
19	564.5
20	661.0
21	764.0
22	969.0
23	1221.0
24	1532.0
25	2030.5
26	2790.0
27	3715.5
28	4625.5
29	5834.0
30	7318.0
31	8665.0
32	9973.0
33	11457.0
34	12845.5
35	14148.0
36	15455.0
37	16487.5
38	16951.0
39	17558.5
40	18690.0
41	19611.0
42	19846.0
43	20309.0
44	20755.0
45	20804.5
46	21490.0
47	21665.0
48	21404.5
49	21582.5
50	21240.5
51	20594.5
52	19838.5
53	19218.0
54	18496.0
55	17208.5
56	15835.0
57	14351.5
58	12932.5
59	11295.5
60	9497.5
61	7952.0
62	6305.5
63	4577.0
64	3182.5
65	2283.0
66	1655.0
67	1167.5
68	878.5
69	590.5
70	378.5
71	292.0
72	204.5
73	132.5
74	97.5
75	60.0
76	42.5
77	29.5
78	19.5
79	18.0
80	12.5
81	8.5
82	9.5
83	7.5
84	4.5
85	5.5
86	4.0
87	1.0
88	2.0
89	3.5
90	3.0
91	2.5
92	2.0
93	3.0
94	3.5
95	2.0
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04332537879378679
2	0.06204194243270268
3	0.04661810758211459
4	0.057016198492623425
5	0.08977018486072623
6	0.07140022425216064
7	0.09600903940703154
8	0.1128192863790208
9	0.11247268334867051
10-11	0.06585457576655593
12-13	0.04852442424904121
14-15	0.10025492652882265
16-17	0.052163756067719294
18-19	0.02729498864008568
20-21	0.03431370000467914
22-23	0.08058520455644344
24-25	0.17373476896308504
26-27	0.17572773638759923
28-29	0.13474192804867693
30-31	0.1541516977482934
32-33	0.2051023432097867
34-35	0.17564108563001166
36-37	0.17538113335724895
38-39	0.21307421290784348
40-41	0.1528519363844798
42-43	0.08517769470858484
44-45	0.07183347804009851
46-47	0.08093180758679373
48-49	0.12659675683544502
50-51	0.13465527729108934
52-53	0.15865753714284725
54-55	0.23213737957710964
56-57	0.18915860381367314
58-59	0.1447067651712479
60-61	0.240802455335867
62-63	0.2436619303362569
64-65	0.2636782553389864
66-67	0.1407208303222195
68-69	0.13890116441288047
70-71	0.14418686062572245
72-73	0.15900414017319753
74-75	0.16247017047670048
76-77	0.1531118886572425
78-79	0.18742558866192166
80-81	0.27537610761330883
82-83	0.2922730053428857
84-85	0.2283247462432564
86-87	0.23672986972925106
88-89	0.23213737957710964
90-91	0.18049352805491578
92-93	0.16740926365919218
94-95	0.15510485608175673
96-97	0.13994097350393134
98-99	0.15423834850588097
100-101	0.17858721138798916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	577029.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.21362668054424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74829453890165	48.74607375067296
2	14.99078343786926	19.552067095321828
3	5.0856019195745645	9.949516350869844
4	2.1911028306888065	5.715590480768943
5	1.1174484344480449	3.6436432519426707
6	0.5991983921326769	2.344554015127365
7	0.3596930818932946	1.6419823253514616
8	0.23839097890802452	1.2437072242013927
9	0.14211403248066445	0.834099431423471
>10	0.5113683253420018	5.11983737272844
>50	0.013870162099986448	0.5868963814400847
>100	0.0013336660381548216	0.1139348908579042
>500	5.334664152446294E-4	0.2617893293690452
>1k	2.667332076223147E-4	0.24630809992462988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1416	0.24539494548800841	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	922	0.1597839969914857	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.1010347833471108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.7330151517514717E-4	0.0
22-23	0.0	0.0	0.0	3.4660303035029434E-4	0.0
24-25	0.0	0.0	0.0	8.665075758757359E-4	0.0
26-27	0.0	0.0	0.0	0.0012131106062260302	0.0
28-29	0.0	0.0	0.0	0.0018196659093390453	0.0
30-31	0.0	0.0	0.0	0.003725982576265664	0.0
32-33	0.0	0.0	0.0	0.006325505303892871	0.0
34-35	0.0	0.0	0.0	0.009358281819457949	0.0
36-37	0.0	0.0	0.0	0.015337184093000526	0.0
38-39	0.0	0.0	0.0	0.02339570454864487	0.0
40-41	0.0	0.0	0.0	0.03188747879222708	0.0
42-43	0.0	0.0	0.0	0.04011930076304657	0.0
44-45	0.0	0.0	0.0	0.047744567430753046	0.0
46-47	0.0	0.0	0.0	0.0584026106140246	0.0
48-49	0.0	0.0	0.0	0.06992716137317188	0.0
50-51	0.0	0.0	0.0	0.08223156895060735	0.0
52-53	0.0	0.0	0.0	0.09236970758835344	0.0
54-55	0.0	0.0	0.0	0.10155468789263625	0.0
56-57	0.0	0.0	0.0	0.11290593713660838	0.0
58-59	0.0	0.0	0.0	0.12711666138097044	0.0
60-61	0.0	0.0	0.0	0.14115408411015737	0.0
62-63	0.0	0.0	0.0	0.15441165002105614	0.0
64-65	0.0	0.0	0.0	0.16740926365919218	0.0
66-67	0.0	0.0	0.0	0.18127338487320394	0.0
68-69	0.0	0.0	0.0	0.19747707654208022	0.0
70-71	0.0	0.0	0.0	0.21662689396893398	0.0
72-73	0.0	0.0	0.0	0.23698982200201377	0.0
74-75	0.0	0.0	0.0	0.25743940079268113	0.0
76-77	0.0	0.0	0.0	0.27676251973471006	0.0
78-79	0.0	0.0	0.0	0.30033152579853006	0.0
80-81	0.0	0.0	0.0	0.32268742125612404	0.0
82-83	0.0	0.0	0.0	0.3467763318654695	0.0
84-85	0.0	0.0	0.0	0.3753710818693688	0.0
86-87	0.0	0.0	0.0	0.40179956293357877	0.0
88-89	0.0	0.0	0.0	0.43481350157444426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	645	0.0	61.077885	1
GTATCAA	1310	0.0	49.27575	1
CAACGCA	1655	0.0	38.723698	5
TCAACGC	1660	0.0	38.32108	4
ATCAACG	1700	0.0	37.419407	3
AACGCAG	1785	0.0	36.160015	6
TATCAAC	1850	0.0	34.385403	2
ACGCAGA	1945	0.0	32.453384	7
AACCGTC	60	0.0039656423	31.640013	7
CGCAGAG	1975	0.0	31.479809	8
GTACATG	2250	0.0	29.533243	1
TACATGG	2235	0.0	28.887	2
ACATGGG	2220	0.0	28.440662	3
GCAGAGT	2400	0.0	25.705276	9
ATACGTA	50	0.0016546469	23.747534	24-25
CATGGGA	1015	0.0	23.38541	4
TATAGGC	145	2.3422317E-5	22.917704	3
GAGTACT	1255	0.0	22.120878	12-13
CATGGGG	1450	0.0	21.608118	4
AGAGTAC	2135	0.0	21.56076	10-11
>>END_MODULE