FastQCFastQC Report
Fri 10 Feb 2017
SRR2031471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577029
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.27346979094638224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.19461760154169028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.11767172880392492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.059.228051
GTATCAA18150.046.3666421
TCAACGC22100.038.039794
ATCAACG22000.037.996813
CAACGCA22200.037.8684435
AACGCAG22650.036.9063956
TATCAAC22850.036.7912252
ACGCAGA24800.033.13237
CGCAGAG25350.032.4134568
GGTATAC650.00581444829.2587411
GTACATG23200.027.6665361
TACATGG22400.027.564652
ACATGGG22400.026.7165053
GCAGAGT30700.026.6101449
GAGTACT16850.024.2412912-13
CAGAGTA28800.022.51113110-11
CATGGGG14500.021.9464324
GTATTAC1302.9200997E-421.9440561
AGAGTAC27350.021.70750210-11
GTACTTT18950.021.68399814-15
Produced by FastQC (version 0.11.5)