##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577029
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.291572867221575	34.0	33.0	34.0	31.0	34.0
2	33.39210680918983	34.0	34.0	34.0	31.0	34.0
3	33.483310197581055	34.0	34.0	34.0	31.0	34.0
4	36.66151614563566	37.0	37.0	37.0	35.0	37.0
5	36.571588256396126	37.0	37.0	37.0	35.0	37.0
6	36.61603662900825	37.0	37.0	37.0	37.0	37.0
7	36.62324250600923	37.0	37.0	37.0	36.0	37.0
8	36.62872576594937	37.0	37.0	37.0	37.0	37.0
9	38.56771323451681	39.0	39.0	39.0	38.0	39.0
10-11	38.57523625329056	39.0	39.0	39.0	38.0	39.0
12-13	38.57266792483567	39.0	39.0	39.0	38.0	39.0
14-15	40.28738243658464	41.0	41.0	41.0	39.0	41.0
16-17	40.34480935966823	41.0	41.0	41.0	39.0	41.0
18-19	40.3692422737852	41.0	41.0	41.0	39.0	41.0
20-21	40.360517409003705	41.0	41.0	41.0	39.0	41.0
22-23	40.32225416746819	41.0	41.0	41.0	39.0	41.0
24-25	40.29063703903964	41.0	41.0	41.0	39.0	41.0
26-27	40.21312273733209	41.0	40.0	41.0	39.0	41.0
28-29	40.14652383155786	41.0	40.0	41.0	38.5	41.0
30-31	40.012367662630474	41.0	40.0	41.0	38.0	41.0
32-33	39.89456249165987	41.0	40.0	41.0	38.0	41.0
34-35	39.81430742648983	41.0	40.0	41.0	38.0	41.0
36-37	39.72578154650806	41.0	40.0	41.0	37.0	41.0
38-39	39.62393397905478	41.0	40.0	41.0	37.0	41.0
40-41	39.51418732853981	41.0	40.0	41.0	37.0	41.0
42-43	39.33094263893149	41.0	40.0	41.0	36.0	41.0
44-45	39.19505952040539	41.0	40.0	41.0	36.0	41.0
46-47	39.029553973890394	41.0	39.0	41.0	35.0	41.0
48-49	38.81428403078528	41.0	39.0	41.0	35.0	41.0
50-51	38.7084531280057	41.0	39.0	41.0	35.0	41.0
52-53	38.72148280242414	41.0	39.0	41.0	35.0	41.0
54-55	38.64289749735282	41.0	39.0	41.0	35.0	41.0
56-57	38.55101650003726	41.0	38.5	41.0	35.0	41.0
58-59	38.48789142313471	41.0	38.0	41.0	35.0	41.0
60-61	38.29871029012406	40.5	37.0	41.0	35.0	41.0
62-63	38.085988745799604	40.0	37.0	41.0	35.0	41.0
64-65	37.80617698590538	39.5	36.0	41.0	35.0	41.0
66-67	37.50324160484135	39.0	36.0	41.0	35.0	41.0
68-69	37.15052103100537	39.0	35.0	41.0	34.0	41.0
70-71	36.79722596264659	37.0	35.0	40.5	34.0	41.0
72-73	36.44272644875734	37.0	35.0	39.5	34.0	41.0
74-75	36.06165548005386	36.5	35.0	39.0	34.0	41.0
76-77	34.898699198827096	35.5	34.5	37.5	32.0	39.0
78-79	35.275052900287506	35.5	35.0	37.0	33.0	39.0
80-81	35.05787750702304	35.0	35.0	37.0	33.0	39.0
82-83	34.778182725651575	35.0	35.0	36.5	33.0	39.0
84-85	34.518723495699525	35.0	35.0	36.0	33.0	37.0
86-87	34.316537816990135	35.0	35.0	36.0	33.0	37.0
88-89	34.144886998747026	35.0	35.0	36.0	33.0	37.0
90-91	34.0017026873866	35.0	35.0	35.0	33.0	36.0
92-93	33.90850373204813	35.0	35.0	35.0	33.0	36.0
94-95	33.8274584119689	35.0	35.0	35.0	33.0	36.0
96-97	33.755778305769724	35.0	35.0	35.0	33.0	36.0
98-99	33.69621717452676	35.0	35.0	35.0	32.5	36.0
100-101	33.453603718357314	35.0	34.5	35.0	31.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.00733024956952022
1101	2	0.04857337494286895
1101	3	0.13486894432841012
1101	4	0.038285234673679724
1101	5	0.10681779518740342
1101	6	-0.008761276570460552
1101	7	-0.15035643658911368
1101	8	0.04704813593527035
1101	9	0.16866646713976507
1101	10-11	0.09012048389408989
1101	12-13	0.12045657144822286
1101	14-15	0.21669006402122903
1101	16-17	0.170085226359042
1101	18-19	0.18676022704539008
1101	20-21	0.1403180233281205
1101	22-23	0.03674302029071441
1101	24-25	0.040611942265911694
1101	26-27	-0.021518299593864754
1101	28-29	-0.02216790452227002
1101	30-31	0.037451677738097544
1101	32-33	0.15016194179087705
1101	34-35	0.09311621945078485
1101	36-37	-0.0364738215082312
1101	38-39	0.0730106078637931
1101	40-41	0.09132255444713167
1101	42-43	0.14816997249473474
1101	44-45	0.23270084502026833
1101	46-47	0.2707680611445511
1101	48-49	0.18907468692336238
1101	50-51	0.17792967345700106
1101	52-53	0.2851924614947947
1101	54-55	0.06269304987760194
1101	56-57	0.07787757389478145
1101	58-59	0.058956586862258575
1101	60-61	0.1692242319486965
1101	62-63	0.06428928642990428
1101	64-65	0.16522447914513805
1101	66-67	0.06484322834644729
1101	68-69	0.0703197089027654
1101	70-71	0.0180092111882999
1101	72-73	0.03444005455610011
1101	74-75	0.16066516258523222
1101	76-77	0.14563043502555217
1101	78-79	0.21860830591985092
1101	80-81	0.23603417962378614
1101	82-83	0.28225135180452554
1101	84-85	0.2824199261071456
1101	86-87	0.3499541624074425
1101	88-89	0.2235860309285158
1101	90-91	0.2629875176782406
1101	92-93	0.04238409008078747
1101	94-95	0.20805194935675075
1101	96-97	0.28405036261106886
1101	98-99	0.2537681351053749
1101	100-101	0.21946838603103203
1102	1	0.18958791163435507
1102	2	0.12226952006758296
1102	3	0.07279411439643724
1102	4	0.0633058489800078
1102	5	0.027890769831806494
1102	6	-0.006983292649614725
1102	7	-0.0023972529156424116
1102	8	0.08276242164955505
1102	9	0.04358916349103481
1102	10-11	0.07804823075040446
1102	12-13	0.10424857309737234
1102	14-15	0.08729663998494885
1102	16-17	0.06688485530152377
1102	18-19	0.005070884641760642
1102	20-21	-0.009676823095290388
1102	22-23	0.03545462614518158
1102	24-25	-0.006053693685444728
1102	26-27	-0.04643584236855247
1102	28-29	0.01634219648783386
1102	30-31	0.010936526222948828
1102	32-33	0.20944095642703786
1102	34-35	0.22173660699974107
1102	36-37	0.16713110531098607
1102	38-39	0.09082909889657031
1102	40-41	0.1651604229278547
1102	42-43	0.26768141343474383
1102	44-45	0.3694123478031983
1102	46-47	0.36679207681142856
1102	48-49	0.20832329545768857
1102	50-51	0.3605595435868665
1102	52-53	0.4211953474976866
1102	54-55	0.14214831683287343
1102	56-57	0.24936283466576015
1102	58-59	0.35597008923290474
1102	60-61	0.27716589346179177
1102	62-63	0.29900893186383826
1102	64-65	0.5177420013055141
1102	66-67	0.4400622553511937
1102	68-69	0.45688948832968634
1102	70-71	0.48770618088526874
1102	72-73	0.268476851092899
1102	74-75	0.3652235010721441
1102	76-77	0.30299489596144014
1102	78-79	0.5629960610218845
1102	80-81	0.41280185639146083
1102	82-83	0.4176229246761025
1102	84-85	0.4614422926295063
1102	86-87	0.5294532347636505
1102	88-89	0.5424893498318397
1102	90-91	0.4959549470742459
1102	92-93	0.4223186396581937
1102	94-95	0.23067615055237667
1102	96-97	0.3141601337922708
1102	98-99	0.28154591288315345
1102	100-101	0.26743530006937277
1103	1	-0.19453082677009803
1103	2	-0.1370254706559777
1103	3	-0.03458993013046552
1103	4	-0.003929772541326315
1103	5	-0.12398307061346259
1103	6	-0.1663153891245699
1103	7	-0.13827851451119244
1103	8	-0.09879601990888887
1103	9	-0.11388764541434426
1103	10-11	-0.017870136596535247
1103	12-13	0.008905344896994905
1103	14-15	0.05520016503133007
1103	16-17	-0.01686282558901553
1103	18-19	-0.08359330830814571
1103	20-21	-0.09414806613796856
1103	22-23	-0.022968379420682083
1103	24-25	-0.11661027995631912
1103	26-27	-0.11291079598635889
1103	28-29	-0.0756058554602177
1103	30-31	-0.12075178546536591
1103	32-33	-0.005642531513593951
1103	34-35	-0.011324617490053868
1103	36-37	-0.020178727713137334
1103	38-39	0.018064720417903857
1103	40-41	-0.1514444008198268
1103	42-43	0.03280200712676873
1103	44-45	-0.25843551861608915
1103	46-47	-0.18916339628690793
1103	48-49	-0.1496915468428739
1103	50-51	-0.12658331355599017
1103	52-53	-0.07010335380101651
1103	54-55	-0.09109843641388693
1103	56-57	-0.1887818777646686
1103	58-59	-0.09469781429213953
1103	60-61	-0.14613651841204955
1103	62-63	-0.23609310895248825
1103	64-65	-0.08327840685775101
1103	66-67	-0.2205499895467682
1103	68-69	-0.20338880480574772
1103	70-71	-0.22696914379005761
1103	72-73	-0.13191275929671065
1103	74-75	-0.30056499614492793
1103	76-77	-0.398099723704604
1103	78-79	-0.23353455122300915
1103	80-81	-0.13174359815399583
1103	82-83	-0.12471473766156294
1103	84-85	-0.21024601905879337
1103	86-87	-0.2340718116185343
1103	88-89	-0.19439376705128097
1103	90-91	-0.04437900468828104
1103	92-93	-0.028182287985593746
1103	94-95	-0.10898989768509892
1103	96-97	-0.1565448754841725
1103	98-99	-0.14936317802593635
1103	100-101	-0.06521776115511813
1104	1	0.059844581993800716
1104	2	0.07069853196011167
1104	3	-0.050168739809997476
1104	4	0.05011862510419718
1104	5	0.0672922727482117
1104	6	0.1706237868074112
1104	7	0.1731276332978311
1104	8	0.1004910958530516
1104	9	0.15110908447447002
1104	10-11	0.08665896739299939
1104	12-13	0.1330322252109326
1104	14-15	0.13060892892930553
1104	16-17	0.0209331194731206
1104	18-19	0.048071178284395444
1104	20-21	0.06474990639310363
1104	22-23	0.06623371345047246
1104	24-25	0.10436094306526655
1104	26-27	0.11060457353620023
1104	28-29	0.02317439306823843
1104	30-31	0.05830642251370932
1104	32-33	0.0934287569102068
1104	34-35	0.02379310651256361
1104	36-37	0.09071728275122126
1104	38-39	0.02806799052908815
1104	40-41	0.12865697262687092
1104	42-43	0.23291646101819907
1104	44-45	0.10095624070372367
1104	46-47	0.119296381345535
1104	48-49	0.20718876708780698
1104	50-51	0.2006043908259656
1104	52-53	0.1574375225887863
1104	54-55	0.21664845698049362
1104	56-57	0.34041040477293905
1104	58-59	0.12040028904336708
1104	60-61	0.2695011532687914
1104	62-63	0.1863202331011422
1104	64-65	0.08697993192577513
1104	66-67	0.08039507258533973
1104	68-69	0.15152617230347687
1104	70-71	0.2957156174918296
1104	72-73	0.2785090850460037
1104	74-75	0.15526234198456734
1104	76-77	-0.12281195392043287
1104	78-79	0.12045825453238734
1104	80-81	0.04548661767334039
1104	82-83	0.011988990265187738
1104	84-85	-0.010481467064032302
1104	86-87	-0.021063309329456104
1104	88-89	-0.021417965874036327
1104	90-91	-0.014447677023142091
1104	92-93	0.026932165905840577
1104	94-95	-0.11691664719457862
1104	96-97	-0.07769595462086443
1104	98-99	0.030646632307615107
1104	100-101	0.009700486065291614
1105	1	0.05821865760242417
1105	2	0.07279842272812687
1105	3	0.05929552124189286
1105	4	-0.025113335923840907
1105	5	-0.07539985627109047
1105	6	-0.12129555729845976
1105	7	-0.049197124696817696
1105	8	-0.0695686678543126
1105	9	0.09692625272939637
1105	10-11	0.06945336015105141
1105	12-13	0.10949712658825916
1105	14-15	0.15389919723821777
1105	16-17	0.04664344126804565
1105	18-19	0.09986377562014326
1105	20-21	0.1164357828228475
1105	22-23	0.06813585665737776
1105	24-25	0.02236005163182142
1105	26-27	0.04890262619355212
1105	28-29	0.10988042091611305
1105	30-31	0.198851578012345
1105	32-33	0.07592338947640798
1105	34-35	0.2573157125115344
1105	36-37	0.2029562873590578
1105	38-39	0.3390134749792253
1105	40-41	0.2740751930243732
1105	42-43	0.38607822480089027
1105	44-45	0.3789704785281245
1105	46-47	0.19015121580257954
1105	48-49	0.3518458163175353
1105	50-51	0.4253586188571532
1105	52-53	0.4023494464528312
1105	54-55	0.4533946621106253
1105	56-57	0.4391445064967954
1105	58-59	0.4232412623380313
1105	60-61	0.44204688631585753
1105	62-63	0.4485554710887598
1105	64-65	0.3528075839402831
1105	66-67	0.40001006122270155
1105	68-69	0.40005875984600436
1105	70-71	0.3768972690292429
1105	72-73	0.3350664569495194
1105	74-75	0.27102313649032794
1105	76-77	0.28292994470726285
1105	78-79	0.47468534406494456
1105	80-81	0.48967001441145186
1105	82-83	0.5152408390479408
1105	84-85	0.5691574396116721
1105	86-87	0.5361296797347848
1105	88-89	0.5956046464026343
1105	90-91	0.6127652123512277
1105	92-93	0.5694918354476926
1105	94-95	0.41039134934012367
1105	96-97	0.4262998016536983
1105	98-99	0.505804202586468
1105	100-101	0.5988469699253187
1106	1	-0.11381854935678604
1106	2	-0.011645901771252909
1106	3	0.02692834540842881
1106	4	-0.04870952886746949
1106	5	0.0046454829635393935
1106	6	-0.2886459108365358
1106	7	-0.08199610276486169
1106	8	-0.11262294899354686
1106	9	-0.1279707444458964
1106	10-11	-0.06346526311949674
1106	12-13	-0.037639484843708715
1106	14-15	-0.013622078017711203
1106	16-17	-0.0333389268073816
1106	18-19	-0.13440243045747735
1106	20-21	-0.12046171812172446
1106	22-23	-0.09625485239787679
1106	24-25	-0.09612918029995399
1106	26-27	-0.12643157074425204
1106	28-29	-0.13245059148020744
1106	30-31	-0.02544719315046251
1106	32-33	-0.1669608695329643
1106	34-35	0.028141174449963557
1106	36-37	0.02852167840995179
1106	38-39	-0.019742214914806766
1106	40-41	0.004788026546620472
1106	42-43	-0.003155506150335441
1106	44-45	0.027159607906867222
1106	46-47	-0.3311690195296393
1106	48-49	-0.05730479270353328
1106	50-51	-0.12076325732355997
1106	52-53	-0.32657320697190073
1106	54-55	-0.11789318805399773
1106	56-57	-0.028303902132051917
1106	58-59	-0.13564126946240407
1106	60-61	-0.15271571241392223
1106	62-63	-0.12080726078004034
1106	64-65	-0.08263798199052275
1106	66-67	-0.07696048626654317
1106	68-69	-0.43058030746117026
1106	70-71	-0.23739088699529987
1106	72-73	-0.19742666120236407
1106	74-75	-0.20059936040609472
1106	76-77	-0.3460035037733391
1106	78-79	-0.08741771273503929
1106	80-81	-0.04689823732925191
1106	82-83	0.0027860433443720467
1106	84-85	0.06071930427766148
1106	86-87	-0.08549011839766507
1106	88-89	-0.31135096792600336
1106	90-91	-0.06799350015844396
1106	92-93	0.055894562998481945
1106	94-95	0.05680829038113444
1106	96-97	-0.05176824318176898
1106	98-99	-0.06725133797595362
1106	100-101	0.03614118917290199
1107	1	0.09163437858292411
1107	2	0.12644784114220897
1107	3	0.02600382467648643
1107	4	0.03670397611272591
1107	5	0.048411945537893075
1107	6	0.08209562456563191
1107	7	0.07063703694242918
1107	8	0.009762663370608493
1107	9	0.09391423122001896
1107	10-11	0.106310758650352
1107	12-13	0.16234481574763748
1107	14-15	0.07184375832466827
1107	16-17	0.05747343037160846
1107	18-19	0.03609750848927007
1107	20-21	0.07878994339903045
1107	22-23	0.11661168518476472
1107	24-25	0.10032711429072094
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2316	16-17	-0.1840439568003731
2316	18-19	-0.29578245417487636
2316	20-21	-0.23295273753709012
2316	22-23	-0.3648003006480707
2316	24-25	-0.2908672069084588
2316	26-27	-0.3257594334949445
2316	28-29	-0.31239770341075257
2316	30-31	-0.6067398779773825
2316	32-33	-0.687966781570843
2316	34-35	-0.6464608666313367
2316	36-37	-0.4812206329112101
2316	38-39	-0.3190293945351499
2316	40-41	-0.2522687653721505
2316	42-43	-0.31095801119244726
2316	44-45	-0.3351517998160034
2316	46-47	-0.15979998891342717
2316	48-49	-0.2450293073191716
2316	50-51	-0.1564756881838818
2316	52-53	-0.1993843247192686
2316	54-55	-0.29572174761150904
2316	56-57	-0.35915513171474345
2316	58-59	-0.2859091722037519
2316	60-61	-0.48533276297353467
2316	62-63	-0.3071922616889253
2316	64-65	-0.19958250578149261
2316	66-67	-0.11488019334800725
2316	68-69	-0.2354657454972937
2316	70-71	-0.3463004925420563
2316	72-73	-0.27350195096146734
2316	74-75	-0.209179605716713
2316	76-77	-0.05875463554339433
2316	78-79	-0.19615419857817784
2316	80-81	-0.11919951754946112
2316	82-83	-0.17241109671812183
2316	84-85	-0.22224052329512034
2316	86-87	-0.17685404198262233
2316	88-89	-0.28683938188984115
2316	90-91	-0.31797186019549173
2316	92-93	-0.18287429622923668
2316	94-95	-0.23774190134894724
2316	96-97	-0.32762799890986116
2316	98-99	-0.49742134152490536
2316	100-101	-0.5304341568500988
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	7.0
15	32.0
16	95.0
17	201.0
18	432.0
19	792.0
20	1207.0
21	1810.0
22	2503.0
23	2812.0
24	2669.0
25	2189.0
26	1795.0
27	1781.0
28	2001.0
29	2329.0
30	2763.0
31	3236.0
32	4210.0
33	6062.0
34	9026.0
35	16453.0
36	39086.0
37	120206.0
38	245572.0
39	107552.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41913996773578	19.262779926798384	13.734496695519436	23.5835834099464
2	14.87578527793423	21.294397989688488	40.60967895671765	23.220137775659634
3	17.347308367517055	27.156347427945565	31.279537076992664	24.216807127544715
4	11.940994300113166	17.961662238812952	39.78170941148538	30.315634049588496
5	12.6360209696287	38.673714310471816	35.5676097222824	13.122654997617087
6	29.107375885787366	38.73531486285785	19.062126860175137	13.095182391179646
7	25.969578652026154	33.329520699999485	23.620130010796682	17.080770637177682
8	25.316058638300674	35.13185645782101	21.546230778695698	18.005854125182616
9	26.725866464250498	15.421062026345297	20.565690805834716	37.28738070356949
10-11	23.885021376741896	26.321120775558942	29.91660731089772	19.877250536801444
12-13	25.172651634493242	24.300598410131897	29.33058823733296	21.1961617180419
14-15	22.37287900608115	25.48294799741434	27.15080177945996	24.99337121704455
16-17	21.61823755825097	28.528722126617552	27.509882518902863	22.343157796228613
18-19	21.638163208008233	27.54252122161641	29.564250631709204	21.25506493866615
20-21	22.963353226165644	26.713124676672873	29.119675013453005	21.20384708370847
22-23	22.683781924305364	26.76971174758981	28.638855239511358	21.907651088593468
24-25	22.18363375150989	26.85038360290384	29.05174609941615	21.914236546170123
26-27	22.03624673428449	27.07994124961548	28.927978787471787	21.955833228628247
28-29	22.107727687863175	27.006528268076647	28.96015624864608	21.925587795414096
30-31	22.525990894738392	26.849950349115904	28.795779761502455	21.82827899464325
32-33	22.266385225006022	27.030270575655642	28.712595034218385	21.99074916511995
34-35	22.34506411289554	27.004535300652133	28.761466061497774	21.88893452495455
36-37	22.544331833653374	26.831591024103485	28.63848585471359	21.985591287529548
38-39	22.38476175951697	27.17539365916724	28.50229280686005	21.93755177445574
40-41	22.39514825078116	27.07359595444943	28.51286503797903	22.01839075679039
42-43	22.57312890686603	26.820922345324067	28.558529987227676	22.047418760582225
44-45	22.491937147006478	27.1519282393086	28.313308343254846	22.042826270430083
46-47	22.62287438217224	27.043674128411105	28.152665748573675	22.180785740842975
48-49	22.68839333295784	27.065383632212193	28.01214867833298	22.234074356496986
50-51	22.587946186413507	27.38779160146197	27.715591417415762	22.308670794708757
52-53	22.756915163709277	27.269166714324584	27.67668522725894	22.297232894707196
54-55	22.658393252332203	27.294295434024978	27.69184910983677	22.35546220380605
56-57	22.677976323546996	27.392384091614115	27.6553691408924	22.27427044394649
58-59	22.630491708389005	27.2892696900849	27.900330832592466	22.179907768933624
60-61	22.67461911603378	27.44383575413379	27.65466289321038	22.226882236622053
62-63	22.51745330763578	27.482200052689233	27.706389262489427	22.293957377185563
64-65	22.59161720650387	27.22289093459993	27.777936651128854	22.407555207767345
66-67	22.652635555936836	27.4073727455726	27.57548264438604	22.364509054104527
68-69	22.58887999251318	27.39006332601982	27.663973491111133	22.357083190355866
70-71	22.617457980626508	27.16855667557153	27.734129600858193	22.47985574294377
72-73	22.668494465910303	27.185278706167697	27.547834586479198	22.598392241442806
74-75	22.566716376653233	27.510881461178982	27.387140855456586	22.53526130671119
76-77	22.581766063790703	27.352117460295457	27.53720893153002	22.528907544383824
78-79	22.657266792483565	27.321157168877125	27.45503258934993	22.566543449289377
80-81	22.626159170509627	27.51360850147913	27.279911408265445	22.5803209197458
82-83	22.716016006127944	27.328175880241723	27.35356455221488	22.60224356141546
84-85	22.832647925840817	27.211197357498495	27.23493966507749	22.721215051583197
86-87	22.605287625438347	27.401427837369685	27.384357564167328	22.608926973024634
88-89	22.58992531461187	27.418696562773764	27.411851052085673	22.57952707052869
90-91	22.719600156665383	27.33767629188286	27.41210967963315	22.5306138718186
92-93	22.73533912506997	27.39507026509933	27.342299953728492	22.527290656102206
94-95	22.719375096947843	27.3746047344344	27.36359925856022	22.54242091005753
96-97	22.81817724932369	27.145949337035052	27.342126652213324	22.693746761427935
98-99	22.658133300059443	27.251576610534308	27.358330343882198	22.731959745524055
100-101	22.684908384154003	27.265960636293844	27.523053434056173	22.52607754549598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	21.5
2	26.0
3	45.0
4	39.5
5	34.5
6	74.0
7	150.5
8	279.5
9	347.0
10	282.0
11	253.5
12	271.5
13	293.0
14	329.5
15	367.5
16	402.5
17	436.0
18	493.0
19	573.0
20	667.5
21	807.5
22	993.0
23	1215.0
24	1520.0
25	1987.5
26	2738.0
27	3621.5
28	4560.5
29	5820.5
30	7317.0
31	8738.5
32	10229.5
33	11739.5
34	12832.5
35	14054.0
36	15533.5
37	16661.0
38	17147.0
39	17720.5
40	18853.0
41	19675.0
42	19960.0
43	20508.0
44	20795.5
45	21000.5
46	21748.5
47	22003.0
48	21701.5
49	21524.0
50	21228.0
51	20768.5
52	20121.0
53	19299.0
54	18434.0
55	17278.0
56	15873.0
57	14248.0
58	12890.0
59	11304.0
60	9333.0
61	7588.5
62	6036.5
63	4430.5
64	3028.0
65	2168.5
66	1525.5
67	1031.5
68	749.0
69	481.0
70	282.5
71	202.5
72	140.0
73	73.5
74	44.0
75	27.0
76	13.0
77	10.5
78	5.5
79	3.5
80	2.0
81	1.0
82	1.0
83	1.0
84	0.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09340951667940434
2	6.932060607005887E-4
3	0.0
4	0.0
5	6.932060607005887E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.003985934849028385
20-21	0.0035526810610905174
22-23	0.0
24-25	0.0
26-27	0.0019929674245141925
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.002946125757977502
38-39	0.0012131106062260302
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.01880321439650347
48-49	0.0019929674245141925
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0027728242428023547
62-63	0.010571392425683977
64-65	0.008578425001169785
66-67	0.004419188636966252
68-69	0.0025995227276272076
70-71	6.065553031130151E-4
72-73	0.002512871970039634
74-75	0.0026861734852147816
76-77	0.002946125757977502
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.332537879378679E-4
88-89	0.001473062878988751
90-91	5.199045455254415E-4
92-93	0.0
94-95	0.007538615910118902
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	577029.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.2873084147607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0885765307062	47.629551694401115
2	15.320584695526062	19.698383068314936
3	5.196969719957873	10.02297585627313
4	2.3182118008331103	5.96126388043584
5	1.11177678937916	3.5736568673595253
6	0.6085271304706162	2.34723427891883
7	0.39407000188108193	1.7733589823554112
8	0.25846580791179485	1.3292856886316682
9	0.15430978910222937	0.892814490308853
>10	0.5298423512568545	5.28580294025981
>50	0.014878210703896284	0.6319722156752124
>100	0.002975635355912258	0.26590086711816174
>500	2.705123050792071E-4	0.11808154922911467
>1k	5.410246101584142E-4	0.46971762071846646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1578	0.27346979094638224	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.19461760154169028	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.11767172880392492	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.7330151517514717E-4	0.0
22-23	0.0	0.0	0.0	3.4660303035029434E-4	0.0
24-25	0.0	0.0	0.0	8.665075758757359E-4	0.0
26-27	0.0	0.0	0.0	0.0012131106062260302	0.0
28-29	0.0	0.0	0.0	0.0018196659093390453	0.0
30-31	0.0	0.0	0.0	0.0038126333338532378	0.0
32-33	0.0	0.0	0.0	0.006498806819068019	0.0
34-35	0.0	0.0	0.0	0.009531583334633095	0.0
36-37	0.0	0.0	0.0	0.01611704091128869	0.0
38-39	0.0	0.0	0.0	0.02417556136693303	0.0
40-41	0.0	0.0	0.0	0.03258068485292767	0.0
42-43	0.0	0.0	0.0	0.040032650005459	0.0
44-45	0.0	0.0	0.0	0.04748461515799032	0.0
46-47	0.0	0.0	0.0	0.0580560075836743	0.0
48-49	0.0	0.0	0.0	0.06975385985799673	0.0
50-51	0.0	0.0	0.0	0.08240487046578249	0.0
52-53	0.0	0.0	0.0	0.09271631061870374	0.0
54-55	0.0	0.0	0.0	0.10198794168057411	0.0
56-57	0.0	0.0	0.0	0.11368579395489656	0.0
58-59	0.0	0.0	0.0	0.12789651819925862	0.0
60-61	0.0	0.0	0.0	0.14184729017085795	0.0
62-63	0.0	0.0	0.0	0.15527815759693187	0.0
64-65	0.0	0.0	0.0	0.16844907275024307	0.0
66-67	0.0	0.0	0.0	0.18196659093390455	0.0
68-69	0.0	0.0	0.0	0.19834358411795594	0.0
70-71	0.0	0.0	0.0	0.21766670305998487	0.0
72-73	0.0	0.0	0.0	0.23768302806271435	0.0
74-75	0.0	0.0	0.0	0.2581326068533817	0.0
76-77	0.0	0.0	0.0	0.2779756303409361	0.0
78-79	0.0	0.0	0.0	0.30241114398063185	0.0
80-81	0.0	0.0	0.0	0.3256335470141015	0.0
82-83	0.0	0.0	0.0	0.3505023144417352	0.0
84-85	0.0	0.0	0.0	0.37944366747598474	0.0
86-87	0.0	0.0	0.0	0.40604545005536985	0.0
88-89	0.0	0.0	0.0	0.4394059917265857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	875	0.0	59.22805	1
GTATCAA	1815	0.0	46.366642	1
TCAACGC	2210	0.0	38.03979	4
ATCAACG	2200	0.0	37.99681	3
CAACGCA	2220	0.0	37.868443	5
AACGCAG	2265	0.0	36.906395	6
TATCAAC	2285	0.0	36.791225	2
ACGCAGA	2480	0.0	33.1323	7
CGCAGAG	2535	0.0	32.413456	8
GGTATAC	65	0.005814448	29.258741	1
GTACATG	2320	0.0	27.666536	1
TACATGG	2240	0.0	27.56465	2
ACATGGG	2240	0.0	26.716505	3
GCAGAGT	3070	0.0	26.610144	9
GAGTACT	1685	0.0	24.24129	12-13
CAGAGTA	2880	0.0	22.511131	10-11
CATGGGG	1450	0.0	21.946432	4
GTATTAC	130	2.9200997E-4	21.944056	1
AGAGTAC	2735	0.0	21.707502	10-11
GTACTTT	1895	0.0	21.683998	14-15
>>END_MODULE