FastQCFastQC Report
Fri 10 Feb 2017
SRR2031468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289095
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.3583597087462599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.2947128106677736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.1542745464293744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.057.3237571
GTATCAA10050.045.331631
TCAACGC11650.037.9034584
CAACGCA11800.037.421635
ATCAACG12000.036.7979353
TATCAAC12350.036.517682
AACGCAG12450.035.449476
ACACGAC550.002581444334.5317883
ACGCAGA13700.032.215037
TCTATAC600.003953745631.6541393
TACTAGC902.416131E-531.6486592
CGCAGAG13950.031.2975128
GTACATG12450.030.8753451
ACATGGG12000.029.2800793
CATGGGG4450.027.7418294
TACATGG13550.027.3276232
CCTCTCG350.00836893227.15563452-53
GCAGAGT15850.026.6475289
CTAATAC908.951915E-426.378453
CATGGGA7000.024.418914
Produced by FastQC (version 0.11.5)