##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031468_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 289095 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.97724623393694 34.0 33.0 34.0 31.0 34.0 2 33.0541932582715 34.0 33.0 34.0 31.0 34.0 3 33.20723291651533 34.0 34.0 34.0 31.0 34.0 4 36.172168318372854 37.0 37.0 37.0 35.0 37.0 5 36.17389439457618 37.0 37.0 37.0 35.0 37.0 6 36.249758729829296 37.0 37.0 37.0 35.0 37.0 7 36.286922983794256 37.0 37.0 37.0 35.0 37.0 8 36.373465469828254 37.0 37.0 37.0 35.0 37.0 9 38.332793718327885 39.0 39.0 39.0 38.0 39.0 10-11 38.41571109842785 39.0 39.0 39.0 37.5 39.0 12-13 38.46936993029973 39.0 39.0 39.0 38.0 39.0 14-15 40.11607775990591 41.0 40.5 41.0 39.0 41.0 16-17 40.116748819592175 41.0 40.0 41.0 39.0 41.0 18-19 40.15858281879659 41.0 41.0 41.0 39.0 41.0 20-21 40.11546204534841 41.0 41.0 41.0 39.0 41.0 22-23 40.05150210138535 41.0 40.0 41.0 39.0 41.0 24-25 39.95226136737058 41.0 40.0 41.0 38.0 41.0 26-27 39.882464241858216 41.0 40.0 41.0 38.0 41.0 28-29 39.81369273076324 41.0 40.0 41.0 38.0 41.0 30-31 39.65829225687058 41.0 40.0 41.0 38.0 41.0 32-33 39.4831335720092 41.0 40.0 41.0 37.0 41.0 34-35 39.40970615195697 41.0 40.0 41.0 37.0 41.0 36-37 39.310705823345266 41.0 40.0 41.0 36.5 41.0 38-39 39.144177519500516 41.0 40.0 41.0 36.0 41.0 40-41 39.061939846763174 41.0 40.0 41.0 36.0 41.0 42-43 39.202151541880696 41.0 40.0 41.0 36.5 41.0 44-45 39.15388367145748 41.0 40.0 41.0 36.0 41.0 46-47 38.97962953354434 41.0 40.0 41.0 36.0 41.0 48-49 38.78682440028364 41.0 40.0 41.0 35.5 41.0 50-51 37.81286428336706 40.0 38.5 40.5 34.0 40.5 52-53 37.890496203670075 39.5 38.5 40.5 35.0 41.0 54-55 38.34464276448918 40.5 39.0 41.0 35.0 41.0 56-57 38.3714107819229 41.0 39.0 41.0 35.0 41.0 58-59 38.43089122952662 41.0 39.0 41.0 35.0 41.0 60-61 38.29739877894809 41.0 39.0 41.0 35.0 41.0 62-63 38.10259603244608 41.0 38.5 41.0 35.0 41.0 64-65 37.88133485532437 40.5 37.5 41.0 35.0 41.0 66-67 37.65789619329286 40.0 37.0 41.0 34.0 41.0 68-69 37.36285131185251 39.0 36.5 41.0 34.0 41.0 70-71 37.02340580086131 39.0 36.0 41.0 34.0 41.0 72-73 36.67234646050606 39.0 35.5 41.0 34.0 41.0 74-75 36.286575347204206 37.0 35.0 40.0 33.5 41.0 76-77 35.89477680347291 37.0 35.0 39.0 33.0 41.0 78-79 35.46513948701984 36.5 35.0 39.0 33.0 41.0 80-81 35.01282796312631 36.0 35.0 38.0 32.5 39.0 82-83 34.64549023677338 36.0 35.0 37.0 32.0 39.0 84-85 34.2980075753645 35.0 35.0 37.0 32.0 39.0 86-87 34.01630086995624 35.0 35.0 36.0 32.0 37.0 88-89 33.77683979314758 35.0 35.0 36.0 31.0 37.0 90-91 33.541417181203414 35.0 35.0 36.0 31.0 37.0 92-93 33.4273647071032 35.0 35.0 35.5 31.0 36.0 94-95 33.30232795447863 35.0 35.0 35.0 31.0 36.0 96-97 33.21289022639617 35.0 35.0 35.0 31.0 36.0 98-99 33.122015254501115 35.0 35.0 35.0 30.5 36.0 100-101 32.806132932081155 35.0 34.5 35.0 29.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1314198576504424 1101 2 0.20049885274195844 1101 3 -0.07341270254406851 1101 4 0.116178710154486 1101 5 -0.06408128385790945 1101 6 0.1135043377489211 1101 7 0.13199785977506906 1101 8 0.0517424623458993 1101 9 0.3170715645684723 1101 10-11 0.14904877105056613 1101 12-13 0.18226483893297996 1101 14-15 0.19345617238215596 1101 16-17 0.15972658985822363 1101 18-19 0.14944646742596035 1101 20-21 0.20037450513623156 1101 22-23 0.28357486047551816 1101 24-25 0.2911394196996966 1101 26-27 0.21298929528896338 1101 28-29 0.2567132869219506 1101 30-31 0.35630242448888794 1101 32-33 0.3628844867932699 1101 34-35 0.4721244092934356 1101 36-37 0.5566540036816576 1101 38-39 0.5460641115649238 1101 40-41 0.5475237999475127 1101 42-43 0.47118384112727085 1101 44-45 0.19507197985200975 1101 46-47 0.3262712528886311 1101 48-49 0.46316466305717086 1101 50-51 0.4768079813579291 1101 52-53 0.5365101412967661 1101 54-55 0.7226179160691686 1101 56-57 0.7781885162369306 1101 58-59 0.819388175769312 1101 60-61 0.7137803268552005 1101 62-63 0.6498387373707786 1101 64-65 0.6716373361275032 1101 66-67 0.8903227174916424 1101 68-69 0.5000529878427429 1101 70-71 0.7084732278879144 1101 72-73 0.6008306497969471 1101 74-75 0.7673908960029507 1101 76-77 0.6386800788515856 1101 78-79 0.5725037106042947 1101 80-81 0.5544576203678133 1101 82-83 0.5052602672123427 1101 84-85 0.4361468770287047 1101 86-87 0.5090253610013207 1101 88-89 0.4197607234235292 1101 90-91 0.6516821394640786 1101 92-93 0.4208155505781406 1101 94-95 0.3302287607486818 1101 96-97 0.4370751151664152 1101 98-99 0.34552594131420733 1101 100-101 0.31923356005385983 1102 1 0.34360541262969235 1102 2 0.20519931954693504 1102 3 0.28965783687501556 1102 4 0.025913538992661245 1102 5 0.285552403901427 1102 6 0.3628559974999561 1102 7 0.2876157280323284 1102 8 0.1680547030098012 1102 9 0.27914366000415924 1102 10-11 0.04518466316675074 1102 12-13 -0.011054014801459289 1102 14-15 0.20842472788007882 1102 16-17 0.12305484452627269 1102 18-19 0.1341456375504393 1102 20-21 0.08583709590386235 1102 22-23 0.07004399947967244 1102 24-25 0.18280176409803772 1102 26-27 0.08716266147153817 1102 28-29 0.25063509708792253 1102 30-31 -0.04371540486796022 1102 32-33 0.2515239447808213 1102 34-35 0.3413622853307814 1102 36-37 0.3196856426858119 1102 38-39 0.2455746146769613 1102 40-41 0.22797309974004065 1102 42-43 0.24052058905257923 1102 44-45 0.13865016968603783 1102 46-47 0.24127384624962644 1102 48-49 0.29124103753849795 1102 50-51 0.26949308249900383 1102 52-53 0.29493046028016323 1102 54-55 0.4940131442849349 1102 56-57 0.465494014162239 1102 58-59 0.5499298640473214 1102 60-61 0.6910884181415042 1102 62-63 0.4270122980554234 1102 64-65 0.41226071527687935 1102 66-67 0.2939064182177873 1102 68-69 0.4242214914734532 1102 70-71 0.5240852610829378 1102 72-73 0.44501827946499617 1102 74-75 0.43269334672909565 1102 76-77 0.4011444201378893 1102 78-79 0.5263743018905984 1102 80-81 0.37625319868731566 1102 82-83 0.5132348522745858 1102 84-85 0.6449529584602374 1102 86-87 0.7204572204411548 1102 88-89 0.708385593755473 1102 90-91 0.728640127015943 1102 92-93 0.7281531413457785 1102 94-95 0.7390802259976397 1102 96-97 0.7698843734651675 1102 98-99 0.6721840715009293 1102 100-101 0.4554984718795865 1103 1 0.011753074843433353 1103 2 0.11914254473777675 1103 3 0.06828379107349036 1103 4 0.12094574319113605 1103 5 0.1111428142067723 1103 6 0.16223576849232302 1103 7 0.049453752841486676 1103 8 0.04138485568155659 1103 9 0.047631255424008145 1103 10-11 -0.02852716889431406 1103 12-13 -0.11920644800756008 1103 14-15 -0.16927386467717298 1103 16-17 -0.11181216406151151 1103 18-19 -0.058125354815977914 1103 20-21 0.007816461361876748 1103 22-23 -0.055850561589032566 1103 24-25 0.06889432134994422 1103 26-27 0.14659133513566047 1103 28-29 0.08809335090471393 1103 30-31 -0.06761509475345662 1103 32-33 0.0529164781777709 1103 34-35 0.1730208015521555 1103 36-37 -0.0537637847951018 1103 38-39 -0.05544518494135531 1103 40-41 -0.13006005865690895 1103 42-43 -0.018395202932154575 1103 44-45 0.006821686861606224 1103 46-47 -0.17893130642212896 1103 48-49 -0.06094765520959555 1103 50-51 -0.10373270471473717 1103 52-53 -0.14395610937632597 1103 54-55 -0.12597731373033128 1103 56-57 -0.1636806041583725 1103 58-59 -0.28471052240305994 1103 60-61 -0.06916921544627996 1103 62-63 -0.2732948345781665 1103 64-65 -0.32821220934144435 1103 66-67 -0.2801185102173278 1103 68-69 -0.21665696749601437 1103 70-71 -0.39719336487126355 1103 72-73 -0.3558226079395794 1103 74-75 -0.26182895766021375 1103 76-77 -0.07175037948042728 1103 78-79 -0.015789343147574186 1103 80-81 -0.41145255035848294 1103 82-83 -0.6395561782597596 1103 84-85 -0.33928785737946043 1103 86-87 -0.2582373167725862 1103 88-89 -0.09305166769490825 1103 90-91 -0.033883727945884345 1103 92-93 -0.18395623365422153 1103 94-95 -0.18415152495656173 1103 96-97 -0.15613542615314913 1103 98-99 -0.3122058378502146 1103 100-101 -0.23215062163186673 1104 1 -0.16376352052049015 1104 2 -0.059939187779072256 1104 3 0.07903512625230746 1104 4 -0.040464093791221956 1104 5 -0.1453513277835512 1104 6 -0.3371211087271533 1104 7 -0.264138507315657 1104 8 -0.2569681907630894 1104 9 0.021417011652509643 1104 10-11 0.03797312470521064 1104 12-13 0.1664728853816939 1104 14-15 0.20826017888740722 1104 16-17 0.2261698285006304 1104 18-19 0.2044295203343225 1104 20-21 0.04340491870606655 1104 22-23 -0.020758427260254564 1104 24-25 0.08613280805408152 1104 26-27 0.18313049572062567 1104 28-29 0.022942217051294733 1104 30-31 0.14434978515035368 1104 32-33 0.0432836173998723 1104 34-35 0.035322621869248394 1104 36-37 0.021551485176658502 1104 38-39 0.1459151595487569 1104 40-41 0.11583653838472685 1104 42-43 0.24222331341155723 1104 44-45 0.03873315961278223 1104 46-47 -0.18002880942803046 1104 48-49 -0.07576045055674285 1104 50-51 -0.20019413132883557 1104 52-53 -0.17289663450005577 1104 54-55 -0.034947478425685574 1104 56-57 0.10966754896077902 1104 58-59 -0.07826810985377364 1104 60-61 -0.17532641702332796 1104 62-63 0.2791686052898612 1104 64-65 0.12589538789593036 1104 66-67 -0.009028277295030307 1104 68-69 -0.02417451356317457 1104 70-71 -0.17944037994270445 1104 72-73 0.07020429136975537 1104 74-75 0.21236224553862115 1104 76-77 -0.03430944890973109 1104 78-79 0.2932870766158686 1104 80-81 0.23068457460306746 1104 82-83 -0.29050827959354564 1104 84-85 -0.09363592661301823 1104 86-87 -0.07081595950390351 1104 88-89 -0.17049203902841015 1104 90-91 0.008688203939023253 1104 92-93 0.10450530480731146 1104 94-95 -0.2594388330682946 1104 96-97 -0.3045919314798837 1104 98-99 -0.04544270276647211 1104 100-101 -0.48851194478708493 1105 1 -0.02331796370482664 1105 2 -0.116670119954847 1105 3 -0.021730063480859485 1105 4 0.05940163952647026 1105 5 0.003593440378296009 1105 6 0.16768250995546907 1105 7 0.10199239618179234 1105 8 0.18840612649734823 1105 9 -0.009946642486944768 1105 10-11 0.06753786601372269 1105 12-13 0.12127194160423471 1105 14-15 0.15805356462384879 1105 16-17 0.14655490013125672 1105 18-19 0.028051331500620336 1105 20-21 0.16232869955154428 1105 22-23 0.13373950659711653 1105 24-25 0.1275860167670757 1105 26-27 0.24478888478114413 1105 28-29 0.2010215517142555 1105 30-31 0.042854601804641845 1105 32-33 0.04014855554594732 1105 34-35 -0.07746139127063856 1105 36-37 -0.023786332403155086 1105 38-39 0.15175510844920126 1105 40-41 0.1282594653984006 1105 42-43 0.008077862184251217 1105 44-45 -0.13179856252036615 1105 46-47 -0.10264296869699763 1105 48-49 0.05735258113280395 1105 50-51 -0.05353711100633518 1105 52-53 -0.13639043607214774 1105 54-55 5.74532185289911E-4 1105 56-57 -0.008760879076199046 1105 58-59 0.004618754171879402 1105 60-61 0.11713379180698524 1105 62-63 -0.22246084473817263 1105 64-65 -0.23133220064838866 1105 66-67 -0.2561311151452017 1105 68-69 -0.35499197605323474 1105 70-71 -0.3444645609811161 1105 72-73 -0.18636239224318984 1105 74-75 -0.29899176225666935 1105 76-77 -0.32629385833185864 1105 78-79 -0.6113314365435656 1105 80-81 -0.2515479022913283 1105 82-83 -0.12127359968270923 1105 84-85 -0.09334914340809064 1105 86-87 0.10156681786108379 1105 88-89 0.1660284985597471 1105 90-91 0.05454092772768604 1105 92-93 0.062485047484564404 1105 94-95 0.2689331508197057 1105 96-97 -0.029006307139816556 1105 98-99 -0.06432476969123968 1105 100-101 -0.031809690931801526 1106 1 0.22218386542689217 1106 2 0.2515826237427419 1106 3 0.24420329142046882 1106 4 0.19197013864300772 1106 5 0.34332666089443364 1106 6 0.22714795554191625 1106 7 0.3470836039064551 1106 8 0.3046368708419678 1106 9 0.34809033832583935 1106 10-11 0.3089521456842306 1106 12-13 0.011044979953780398 1106 14-15 0.18819690882413198 1106 16-17 0.22558707791788635 1106 18-19 0.20451277041757265 1106 20-21 0.09385446915560891 1106 22-23 0.12626121975939242 1106 24-25 0.12609284801412457 1106 26-27 -0.04097872838860184 1106 28-29 0.07430751841658889 1106 30-31 0.24716363796420637 1106 32-33 -0.17916060504435194 1106 34-35 -0.08530674876012512 1106 36-37 0.1650746286997986 1106 38-39 0.13426014789374818 1106 40-41 0.002366674914867417 1106 42-43 0.29233986352809893 1106 44-45 0.5225826434622647 1106 46-47 0.34103346163423964 1106 48-49 0.6256215008252042 1106 50-51 0.6526197214850171 1106 52-53 0.7374430258396032 1106 54-55 0.6419589484807418 1106 56-57 0.6832606225538456 1106 58-59 0.7986050170193479 1106 60-61 0.4955027538058374 1106 62-63 0.7088222849435368 1106 64-65 0.7181364801370194 1106 66-67 0.8691768509100939 1106 68-69 0.8698481304594665 1106 70-71 0.758788058285738 1106 72-73 0.9184393896048562 1106 74-75 0.7444967776731488 1106 76-77 1.0218011072008224 1106 78-79 1.1129673962961917 1106 80-81 1.0549436488621424 1106 82-83 1.1158353767501126 1106 84-85 0.7660876831105838 1106 86-87 0.7795836408957015 1106 88-89 0.9640400955037265 1106 90-91 0.8286182738690897 1106 92-93 0.7426171929192051 1106 94-95 0.8546970310675732 1106 96-97 0.5895139626260075 1106 98-99 0.7611673538435824 1106 100-101 0.7673155610404265 1107 1 -0.0656473509757518 1107 2 -0.0628172620857157 1107 3 0.05836531986821569 1107 4 0.20626536722395628 1107 5 -0.1364554617448306 1107 6 -0.10844502290820657 1107 7 0.061345532454097906 1107 8 0.03476388668327246 1107 9 0.00650335388171186 1107 10-11 0.08323738425518457 1107 12-13 0.10472510934820178 1107 14-15 0.1373548936964042 1107 16-17 0.09168526544463873 1107 18-19 -0.0031842944223470226 1107 20-21 0.2554666664820928 1107 22-23 0.2648250368946421 1107 24-25 0.17482982532252578 1107 26-27 0.153303180179023 1107 28-29 0.22270673824438347 1107 30-31 0.27150834088033804 1107 32-33 0.22015436569919444 1107 34-35 0.32888620114710676 1107 36-37 0.2828021392844491 1107 38-39 0.36805016739804586 1107 40-41 0.40678368647473206 1107 42-43 0.2287486927940776 1107 44-45 0.5794186645839972 1107 46-47 0.4892542884264941 1107 48-49 0.4140354678106064 1107 50-51 0.267885408179275 1107 52-53 0.3376999118107733 1107 54-55 0.25272913067949787 1107 56-57 0.06126357198537136 1107 58-59 0.18389829516976874 1107 60-61 0.1649404589578296 1107 62-63 0.23809063564045374 1107 64-65 0.38780014980068955 1107 66-67 0.3114845432177873 1107 68-69 0.5480389872217515 1107 70-71 0.5466192746883749 1107 72-73 0.5770070124581892 1107 74-75 0.5984166673073261 1107 76-77 0.5490238844235975 1107 78-79 0.8008747270606662 1107 80-81 0.399836850898204 1107 82-83 0.49272039649227395 1107 84-85 0.5575937960452677 1107 86-87 0.5963739934003414 1107 88-89 0.7599108913745241 1107 90-91 0.7957107052472381 1107 92-93 0.6918941064818327 1107 94-95 0.7001373050792736 1107 96-97 0.6602170690433979 1107 98-99 0.8691308189499054 1107 100-101 0.7580866946686982 1108 1 0.22004455736653483 1108 2 0.23686050375746248 1108 3 0.04623678424343325 1108 4 0.15810926267687364 1108 5 0.023833653901427 1108 6 0.13423757644732603 1108 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-0.2503694437497188 2316 92-93 -0.46411864664725755 2316 94-95 -0.4912661704640726 2316 96-97 -0.5338696143538542 2316 98-99 -0.3979154427102074 2316 100-101 -0.37288936164708986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 117.0 3 32.0 4 29.0 5 34.0 6 39.0 7 84.0 8 168.0 9 176.0 10 125.0 11 137.0 12 146.0 13 160.0 14 161.0 15 190.0 16 225.0 17 335.0 18 510.0 19 750.0 20 1014.0 21 1522.0 22 1930.0 23 1814.0 24 1627.0 25 1191.0 26 1064.0 27 1018.0 28 1078.0 29 1163.0 30 1379.0 31 1698.0 32 2053.0 33 2870.0 34 4228.0 35 7026.0 36 14640.0 37 44883.0 38 122109.0 39 71245.0 40 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.425456810631225 21.85700442967885 14.56360741971207 22.153931339977852 2 14.27948354044792 22.85610439959846 42.01426148360968 20.850150576343935 3 16.45088165567842 29.312845005104776 33.146446555572865 21.089826783643947 4 11.187951915655102 19.677130169015157 42.39431793402075 26.740599981308993 5 11.057019791421409 40.046812459316094 37.13869229810396 11.75747545115854 6 26.06413913428785 41.060400454180396 20.58766512503808 12.287795286493672 7 23.183006422796602 35.45556844346727 25.70157367172758 15.659851462008554 8 22.945755762411345 36.538674645390074 23.414644281914892 17.10092531028369 9 25.66654546084186 16.48156805707063 23.00382664104029 34.84805984104722 10-11 21.72375717381221 28.082922000152305 31.969760953693 18.223559872342488 12-13 22.98531740195018 27.110980368406025 30.850240960728158 19.053461268915633 14-15 20.99810420796579 28.254745972524 28.896198893688485 21.850950925821728 16-17 19.314120967644243 30.982059575292848 29.71375271044566 19.990066746617245 18-19 19.146241667517288 30.010778378883373 31.995003538007193 18.847976415592143 20-21 20.76178809459544 28.987154731502052 31.757088774924043 18.49396839897847 22-23 20.460895063544875 29.275474206424768 30.959387365804513 19.304243364225844 24-25 19.761576781459823 29.451851851851853 31.369807234134722 19.416764132553606 26-27 19.72449165244362 29.405231193089765 31.513649792502363 19.356627361964254 28-29 19.768710640626843 29.318706137464233 31.670049811906363 19.24253341000256 30-31 20.214844765042773 29.166709975158252 31.341142720535505 19.277302539263477 32-33 19.769263835156757 29.31659664156856 31.36275080771524 19.551388715559437 34-35 20.14177073063388 29.266073962666926 31.183322965922695 19.4088323407765 36-37 20.22989382052064 29.106484433478418 30.89895622522248 19.764665520778465 38-39 19.67939428817073 29.624480853099627 31.227682750109203 19.468442108620437 40-41 19.83529199811525 29.106266803403642 31.51608276282602 19.542358435655093 42-43 20.04923115272563 29.12496992295082 30.85186858536602 19.973930338957523 44-45 20.420480192741724 29.359535315213027 30.548940058985615 19.671044433059638 46-47 20.202181062835383 29.391725809243553 30.26155444002077 20.144538687900294 48-49 19.916735072752903 29.57913804867742 30.55621941208865 19.94790746648102 50-51 20.07274238802868 29.65256849908206 30.016800027711387 20.257889085177872 52-53 20.462841531001338 29.69074022421377 30.0025813515541 19.843836893230797 54-55 20.70518721945688 29.418516149054323 29.673031278661128 20.203265352827668 56-57 20.699712496772303 29.76845505574166 29.68423168704953 19.847600760436507 58-59 20.380137775260383 29.501642944863914 30.00154161405259 20.11667766582311 60-61 20.720100567861632 29.625731501148728 29.788027222679787 19.866140708309853 62-63 20.46628081576602 29.62457562758568 30.023546383601452 19.885597173046847 64-65 20.322181776560747 29.344416099724086 30.120475659477204 20.212926464237963 66-67 20.362768430278276 29.745140628193532 29.64156178876152 20.250529152766664 68-69 20.707053211721057 29.583583035711193 29.763715155693042 19.945648596874708 70-71 20.506207204757214 29.168406046633283 30.081948613663645 20.243438134945862 72-73 20.54852664240089 29.28368608794929 29.82438208028495 20.34340518936487 74-75 20.404019404019405 29.524255024255027 29.51888426888427 20.5528413028413 76-77 20.13227847772348 29.52741105935382 29.858280483727633 20.48202997919507 78-79 20.308711829101124 29.64545103224543 29.55395005224914 20.491887086404308 80-81 20.489538507969048 29.56761247643281 29.472736638897707 20.470112376700435 82-83 20.563581609003982 29.678934732225876 29.48360325853144 20.2738804002387 84-85 20.610313825158023 29.340277175816286 29.461805976928595 20.587603022097092 86-87 20.189571980657902 29.726946302964947 29.613036671272752 20.470445045104398 88-89 20.57165247302283 29.46596992981775 29.48452106854559 20.47785652861383 90-91 20.39044914127875 29.575673348016473 29.85327832064587 20.1805991900589 92-93 20.603231859124623 29.621043987172357 29.670421056461375 20.10530309724164 94-95 20.485507196163894 29.325147422616123 29.727224851537976 20.46212052968201 96-97 20.610482084431027 29.579611568086438 29.52210715566202 20.287799191820515 98-99 20.532342445345254 29.519280740047808 29.4317984963448 20.516578318262134 100-101 20.33771724627887 29.760011089740257 29.54202838280397 20.3602432811769 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 232.0 1 149.5 2 64.5 3 61.0 4 63.5 5 62.0 6 78.0 7 138.5 8 214.5 9 239.5 10 211.0 11 187.0 12 189.5 13 206.0 14 219.0 15 228.5 16 252.0 17 280.0 18 305.0 19 336.0 20 362.5 21 425.0 22 526.0 23 646.0 24 858.5 25 1191.5 26 1708.0 27 2549.5 28 3545.5 29 5024.5 30 6854.0 31 8383.0 32 10015.0 33 11759.5 34 13305.5 35 14309.5 36 15208.0 37 15439.5 38 14199.5 39 12960.0 40 11777.5 41 10738.0 42 9969.5 43 9198.0 44 8943.5 45 9018.0 46 8842.0 47 8318.5 48 7886.5 49 7505.0 50 6979.5 51 6510.5 52 6057.5 53 5532.0 54 4910.5 55 4279.5 56 3742.5 57 3267.5 58 2838.5 59 2431.5 60 2009.0 61 1545.5 62 1175.5 63 808.5 64 540.0 65 370.5 66 230.5 67 184.5 68 157.0 69 117.5 70 80.5 71 59.0 72 52.5 73 45.0 74 25.0 75 15.5 76 11.0 77 7.5 78 7.5 79 6.0 80 4.0 81 3.5 82 5.0 83 3.5 84 0.5 85 0.5 86 1.5 87 2.0 88 1.5 89 1.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04669745239454159 2 0.07091094622874833 3 0.05188605821615732 4 0.06433871218803508 5 0.09789169650115015 6 0.0771372732146872 7 0.09685397533682699 8 0.11311160691122296 9 0.11414932807554609 10-11 0.06814368979055328 12-13 0.05102129057922136 14-15 0.10290734879537869 16-17 0.057593524619934625 18-19 0.03130458845708158 20-21 0.04047112540860271 22-23 0.08440132136494924 24-25 0.18419550666735848 26-27 0.18592504194123038 28-29 0.1414759854027223 30-31 0.16188450163441084 32-33 0.2168837233435376 34-35 0.1835036925578097 36-37 0.18229301786609936 38-39 0.22172642211037893 40-41 0.16015496636053891 42-43 0.08768743838530586 44-45 0.07298638855739463 46-47 0.08474722841972362 48-49 0.13075286670471642 50-51 0.14009235718362475 52-53 0.1682837821477369 54-55 0.24334561303377783 56-57 0.19941541707743127 58-59 0.1509884294090178 60-61 0.2533769176222349 62-63 0.25182033587575015 64-65 0.2696345491966309 66-67 0.1473564053338868 68-69 0.14458914889569172 70-71 0.15168024351856654 72-73 0.1679378750929625 74-75 0.17122399211331915 76-77 0.15963610577837736 78-79 0.19837769591310814 80-81 0.28485445960670364 82-83 0.29990141648938934 84-85 0.23729223957522613 86-87 0.24507514830764973 88-89 0.24317265950639066 90-91 0.19215136892716927 92-93 0.17278057385980386 94-95 0.16223040868918523 96-97 0.14579982358740207 98-99 0.16084678047008769 100-101 0.18678980957816635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 289095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.581676627283386 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98831488924924 42.027895455230826 2 14.812721078594999 17.05882630235603 3 5.47601184283961 9.459538294246938 4 2.4882079559806214 5.731007436108396 5 1.3242525363925979 3.8126340661709195 6 0.754239610803533 2.6058228821266263 7 0.4702868609219183 1.8955934163366266 8 0.3467443744974824 1.5972897955714944 9 0.2316201359574388 1.200336819216179 >10 1.0385762028434267 10.648013675944448 >50 0.0544930357256374 2.148695958721725 >100 0.013320519844044698 1.1561051110045821 >500 6.054781747293045E-4 0.29704510089743785 >1k 6.054781747293045E-4 0.3611956860677766 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.3583597087462599 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 0.2947128106677736 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.1542745464293744 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.647676369359554E-4 0.0 18-19 0.0 0.0 0.0 0.0010377211643231464 0.0 20-21 0.0 0.0 0.0 0.0010377211643231464 0.0 22-23 0.0 0.0 0.0 0.0019024888012591015 0.0 24-25 0.0 0.0 0.0 0.002767256438195057 0.0 26-27 0.0 0.0 0.0 0.003113163492969439 0.0 28-29 0.0 0.0 0.0 0.004323838184679777 0.0 30-31 0.0 0.0 0.0 0.008993583424133936 0.0 32-33 0.0 0.0 0.0 0.014874003355298432 0.0 34-35 0.0 0.0 0.0 0.02300281914249641 0.0 36-37 0.0 0.0 0.0 0.039087497189505174 0.0 38-39 0.0 0.0 0.0 0.058977152839032154 0.0 40-41 0.0 0.0 0.0 0.07869385496117193 0.0 42-43 0.0 0.0 0.0 0.09616216122727822 0.0 44-45 0.0 0.0 0.0 0.11881907331500026 0.0 46-47 0.0 0.0 0.0 0.1432055206765942 0.0 48-49 0.0 0.0 0.0 0.1681108286203497 0.0 50-51 0.0 0.0 0.0 0.19318909009149243 0.0 52-53 0.0 0.0 0.0 0.21342465279579376 0.0 54-55 0.0 0.0 0.0 0.2343520296096439 0.0 56-57 0.0 0.0 0.0 0.2677320603953718 0.0 58-59 0.0 0.0 0.0 0.30059323059893805 0.0 60-61 0.0 0.0 0.0 0.3272280738165655 0.0 62-63 0.0 0.0 0.0 0.3574949411093239 0.0 64-65 0.0 0.0 0.0 0.3898372507307286 0.0 66-67 0.0 0.0 0.0 0.42511977031771564 0.0 68-69 0.0 0.0 0.0 0.4642072675072208 0.0 70-71 0.0 0.0 0.0 0.5072726958266314 0.0 72-73 0.0 0.0 0.0 0.544111797160103 0.0 74-75 0.0 0.0 0.0 0.5890797142807727 0.0 76-77 0.0 0.0 0.0 0.6430412148255764 0.0 78-79 0.0 0.0 0.0 0.6971756688977672 0.0 80-81 0.0 0.0 0.0 0.7487158200591502 0.0 82-83 0.0 0.0 0.0 0.7997371106383715 0.0 84-85 0.0 0.0 0.0 0.8633840087168578 0.0 86-87 0.0 0.0 0.0 0.9339490478908317 0.0 88-89 0.0 0.0 0.0 1.01039450699597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 505 0.0 57.323757 1 GTATCAA 1005 0.0 45.33163 1 TCAACGC 1165 0.0 37.903458 4 CAACGCA 1180 0.0 37.42163 5 ATCAACG 1200 0.0 36.797935 3 TATCAAC 1235 0.0 36.51768 2 AACGCAG 1245 0.0 35.44947 6 ACACGAC 55 0.0025814443 34.531788 3 ACGCAGA 1370 0.0 32.21503 7 TCTATAC 60 0.0039537456 31.654139 3 TACTAGC 90 2.416131E-5 31.648659 2 CGCAGAG 1395 0.0 31.297512 8 GTACATG 1245 0.0 30.875345 1 ACATGGG 1200 0.0 29.280079 3 CATGGGG 445 0.0 27.741829 4 TACATGG 1355 0.0 27.327623 2 CCTCTCG 35 0.008368932 27.155634 52-53 GCAGAGT 1585 0.0 26.647528 9 CTAATAC 90 8.951915E-4 26.37845 3 CATGGGA 700 0.0 24.41891 4 >>END_MODULE