FastQCFastQC Report
Fri 10 Feb 2017
SRR2031468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289095
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.41647209394835605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9920.3431397983361871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.17260762033241667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT3110.10757709403483284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.057.58061
GGTATAG405.354921E-447.547621
GTATCAA10950.046.8962821
GTATAGA853.5997073E-739.156861
TCAACGC12750.038.371154
ATCAACG12950.037.7785453
CAACGCA13050.037.4890565
TATCAAC13150.037.2039682
AACGCAG13350.036.6466066
ACGCAGA15650.030.350337
CGCAGAG15900.029.8731278
GTACATG12950.029.0058841
CATGGGG4950.027.8272694
TACATGG13150.027.8126752
GCAGAGT17350.027.3765269
ACATGGG12500.027.3590033
GACGAGA350.00838259527.14656416-17
TATAGTT1257.367975E-626.5990315
GTATAGT908.933189E-426.3879284
GAGTACT11050.025.3632612-13
Produced by FastQC (version 0.11.5)