##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031468_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 289095 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.32055206765942 34.0 33.0 34.0 31.0 34.0 2 33.41746830626611 34.0 34.0 34.0 31.0 34.0 3 33.50105328698179 34.0 34.0 34.0 31.0 34.0 4 36.663072692367564 37.0 37.0 37.0 35.0 37.0 5 36.59771355436794 37.0 37.0 37.0 35.0 37.0 6 36.6453103651049 37.0 37.0 37.0 37.0 37.0 7 36.65154361023193 37.0 37.0 37.0 37.0 37.0 8 36.660025251214996 37.0 37.0 37.0 37.0 37.0 9 38.60760303706394 39.0 39.0 39.0 38.0 39.0 10-11 38.61262560749927 39.0 39.0 39.0 38.5 39.0 12-13 38.61156021377056 39.0 39.0 39.0 38.5 39.0 14-15 40.32153098462443 41.0 41.0 41.0 39.0 41.0 16-17 40.36838236565835 41.0 41.0 41.0 39.0 41.0 18-19 40.41549490651862 41.0 41.0 41.0 39.0 41.0 20-21 40.4231083207942 41.0 41.0 41.0 39.0 41.0 22-23 40.37795534339923 41.0 41.0 41.0 39.0 41.0 24-25 40.34749995676162 41.0 41.0 41.0 39.0 41.0 26-27 40.27560144589149 41.0 41.0 41.0 39.0 41.0 28-29 40.21032878465556 41.0 40.0 41.0 39.0 41.0 30-31 40.0635068057213 41.0 40.0 41.0 38.0 41.0 32-33 39.926328715474156 41.0 40.0 41.0 38.0 41.0 34-35 39.84718172227122 41.0 40.0 41.0 38.0 41.0 36-37 39.76546118058078 41.0 40.0 41.0 38.0 41.0 38-39 39.668638336878885 41.0 40.0 41.0 37.0 41.0 40-41 39.57259724312077 41.0 40.0 41.0 37.0 41.0 42-43 39.382166761791105 41.0 40.0 41.0 36.5 41.0 44-45 39.24969300748889 41.0 40.0 41.0 36.0 41.0 46-47 39.07435963956485 41.0 40.0 41.0 36.0 41.0 48-49 38.856035213338174 41.0 40.0 41.0 35.5 41.0 50-51 38.763034642591535 41.0 39.5 41.0 35.0 41.0 52-53 38.78306266106297 41.0 40.0 41.0 35.0 41.0 54-55 38.73528079005171 41.0 39.0 41.0 35.0 41.0 56-57 38.68488905031218 41.0 39.0 41.0 35.0 41.0 58-59 38.67901900759266 41.0 39.0 41.0 35.0 41.0 60-61 38.546074819695946 41.0 39.0 41.0 35.0 41.0 62-63 38.3736678254553 41.0 39.0 41.0 35.0 41.0 64-65 38.150587177225475 40.5 37.5 41.0 35.0 41.0 66-67 37.9036804510628 40.0 37.0 41.0 35.0 41.0 68-69 37.580629204932634 39.0 37.0 41.0 35.0 41.0 70-71 37.247804354969816 39.0 36.0 41.0 34.5 41.0 72-73 36.91326899462115 39.0 36.0 41.0 34.0 41.0 74-75 36.521373596914515 37.0 35.0 40.0 34.0 41.0 76-77 35.27609782251509 36.5 34.5 39.0 32.0 39.5 78-79 35.633151732129576 36.5 35.0 39.0 33.5 40.0 80-81 35.354310866670126 36.0 35.0 38.0 34.0 39.0 82-83 35.00089589927187 36.0 35.0 37.0 33.5 39.0 84-85 34.65534858783445 35.0 35.0 37.0 33.0 39.0 86-87 34.39769452947993 35.0 35.0 36.5 33.0 37.0 88-89 34.15329217039381 35.0 35.0 36.0 33.0 37.0 90-91 33.9737145229077 35.0 35.0 36.0 33.0 37.0 92-93 33.841522682855114 35.0 35.0 36.0 33.0 36.0 94-95 33.718493920683514 35.0 35.0 35.0 33.0 36.0 96-97 33.62443660388453 35.0 35.0 35.0 33.0 36.0 98-99 33.55657136927307 35.0 35.0 35.0 32.0 36.0 100-101 33.326918487002544 35.0 34.5 35.0 31.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13261084973922976 1101 2 0.09159412674630119 1101 3 0.06688688262281772 1101 4 0.13816526482770541 1101 5 0.08604189853002708 1101 6 0.11537868491084424 1101 7 0.11580699384862214 1101 8 0.13620222055228481 1101 9 0.11748928800273717 1101 10-11 0.10370317642723847 1101 12-13 0.19075091958836055 1101 14-15 0.22168046940204533 1101 16-17 0.16803510138594646 1101 18-19 0.10801014963786315 1101 20-21 0.04615045895636172 1101 22-23 -0.014749949746352797 1101 24-25 -0.0416343900945364 1101 26-27 0.014447433639148244 1101 28-29 0.10436082969802385 1101 30-31 0.2229409575093868 1101 32-33 0.2857129285441786 1101 34-35 0.3561277668262264 1101 36-37 0.32592556567599473 1101 38-39 0.358556359259147 1101 40-41 0.3477623163620791 1101 42-43 0.3266972392514518 1101 44-45 0.3967453172166202 1101 46-47 0.2596169093927472 1101 48-49 0.3475825187991646 1101 50-51 0.5601550803129527 1101 52-53 0.4824753217874118 1101 54-55 0.4225197579937401 1101 56-57 0.45148613098891843 1101 58-59 0.5356665635332902 1101 60-61 0.657888477109644 1101 62-63 0.8121779220827747 1101 64-65 0.9851403970860062 1101 66-67 0.8327329827288494 1101 68-69 0.6896195891937325 1101 70-71 0.754280284442217 1101 72-73 0.6865913722065926 1101 74-75 0.6939996594323645 1101 76-77 0.6777563872344032 1101 78-79 0.8208934301198383 1101 80-81 0.7294167337031539 1101 82-83 0.7229438767280314 1101 84-85 0.7856425065660844 1101 86-87 0.6667299205489385 1101 88-89 0.7845194707279504 1101 90-91 0.5508559889747957 1101 92-93 0.6502272548152987 1101 94-95 0.641637948452292 1101 96-97 0.6996417002008073 1101 98-99 0.6986868234192087 1101 100-101 0.599871597686203 1102 1 -0.16611191997031227 1102 2 0.06656819313634088 1102 3 0.026009029925724292 1102 4 0.088064772089119 1102 5 -0.04221601122100793 1102 6 -0.08072803189413236 1102 7 -0.09641097814307642 1102 8 -0.03084264459294417 1102 9 -0.1306305252752722 1102 10-11 0.037831807132633344 1102 12-13 0.16469574583317836 1102 14-15 0.22217482884187945 1102 16-17 0.08247039702909831 1102 18-19 0.14024076426441923 1102 20-21 0.1303698572966141 1102 22-23 0.09276107203788087 1102 24-25 0.04596448179342616 1102 26-27 -7.399366928027007E-4 1102 28-29 0.18110000241586732 1102 30-31 0.09459390097411102 1102 32-33 0.16772310748608987 1102 34-35 0.27072514753161414 1102 36-37 -0.00547355258127169 1102 38-39 -0.07930022787778768 1102 40-41 -0.02970137658397931 1102 42-43 0.1073799415336083 1102 44-45 0.19853149460251274 1102 46-47 0.275071720077392 1102 48-49 0.2874489607078772 1102 50-51 0.3226842556241536 1102 52-53 0.3463076609990239 1102 54-55 0.28486091463274477 1102 56-57 0.28684012476485066 1102 58-59 0.43830206664532767 1102 60-61 0.30978649318847573 1102 62-63 0.34395469853505745 1102 64-65 0.3361193908619384 1102 66-67 0.3150819194508401 1102 68-69 0.13011005859207359 1102 70-71 0.24597342500238284 1102 72-73 0.22933320311945238 1102 74-75 0.24702578754439486 1102 76-77 -3.588876618607628E-4 1102 78-79 0.051832226800335945 1102 80-81 -0.04083579482381339 1102 82-83 0.05428626521351276 1102 84-85 0.03561819380674791 1102 86-87 0.1273108334120039 1102 88-89 0.05915640018853452 1102 90-91 0.03718411397479571 1102 92-93 -5.361658278602022E-4 1102 94-95 0.13085929181328737 1102 96-97 -0.09422662189462727 1102 98-99 -0.07115238819905301 1102 100-101 0.15893539436670068 1103 1 -0.12716869477947057 1103 2 -0.03958638701632822 1103 3 0.03310588107076029 1103 4 -0.0509798366895069 1103 5 -0.027575028396583434 1103 6 -0.024639052886499258 1103 7 -0.05159346860109082 1103 8 0.06571031342232203 1103 9 0.08418340602244001 1103 10-11 0.036877608659352745 1103 12-13 0.15320063610035817 1103 14-15 0.13380710460523915 1103 16-17 0.11816338367032131 1103 18-19 0.007443173615563126 1103 20-21 0.0911284450828731 1103 22-23 0.13346360066383767 1103 24-25 0.22208864686975716 1103 26-27 0.2371834888797153 1103 28-29 0.028800980469306126 1103 30-31 0.17866773208098152 1103 32-33 0.20751616569219777 1103 34-35 0.28357700821863574 1103 36-37 0.2157215810065125 1103 38-39 0.18745788280923392 1103 40-41 0.22149137150761788 1103 42-43 0.3259212106175795 1103 44-45 0.37598259193075734 1103 46-47 0.4376283756117445 1103 48-49 0.5115066897155103 1103 50-51 0.5631720895936212 1103 52-53 0.5878093308463548 1103 54-55 0.752149798220529 1103 56-57 0.6888558690396565 1103 58-59 0.47423360290486727 1103 60-61 0.7049886639900009 1103 62-63 0.734654245290784 1103 64-65 0.5144948679611758 1103 66-67 0.4005721389164876 1103 68-69 0.23073326946993689 1103 70-71 0.26200097748329654 1103 72-73 0.18475424319578337 1103 74-75 0.11539112628485526 1103 76-77 0.10815136997172914 1103 78-79 0.33642192145682515 1103 80-81 0.3249949542219852 1103 82-83 0.36131453334328256 1103 84-85 0.3508764237685824 1103 86-87 0.19601312348833488 1103 88-89 0.2972289192725057 1103 90-91 0.3867338515702201 1103 92-93 0.3623127129889312 1103 94-95 0.42269493112626577 1103 96-97 0.4377241176091857 1103 98-99 0.4696395965337672 1103 100-101 0.3634916920766216 1104 1 0.15528803424214033 1104 2 -0.05419645887830882 1104 3 7.400280942206905E-4 1104 4 0.0530802253858198 1104 5 -0.0017941934554386307 1104 6 0.024168373783524544 1104 7 0.06757997881663158 1104 8 0.07358157701877843 1104 9 0.024303082783333707 1104 10-11 0.08865598295680854 1104 12-13 0.14900635709691556 1104 14-15 0.15282815988583565 1104 16-17 0.014690520655477712 1104 18-19 -0.02214047428869037 1104 20-21 0.1329454067471687 1104 22-23 0.06332826526132607 1104 24-25 -0.13415943075236214 1104 26-27 0.0662386576295404 1104 28-29 0.13052622723271412 1104 30-31 0.08083617432576062 1104 32-33 -0.03420712374101953 1104 34-35 -0.09115385887864136 1104 36-37 -0.005044294339512589 1104 38-39 0.08287353585847512 1104 40-41 -0.3045411201737238 1104 42-43 -0.1152190740341581 1104 44-45 -0.06742260338283046 1104 46-47 -0.1330312446661992 1104 48-49 -0.08712178105036372 1104 50-51 -0.2062334412622988 1104 52-53 -0.3846761988910856 1104 54-55 -0.26715087566029183 1104 56-57 -0.43686065362342674 1104 58-59 -0.14653367053416133 1104 60-61 -0.010890876317013465 1104 62-63 -0.15685373782025636 1104 64-65 -0.3024737867937404 1104 66-67 -0.4014788635161963 1104 68-69 -0.487914494979357 1104 70-71 -0.38749011666428856 1104 72-73 -0.4627026256351243 1104 74-75 -0.43142401785853934 1104 76-77 -0.705301080885306 1104 78-79 -0.38463755341944506 1104 80-81 -0.2997429033402952 1104 82-83 -0.30712771143483764 1104 84-85 -0.2173365337573543 1104 86-87 -0.10712140102022971 1104 88-89 -0.3188197902876553 1104 90-91 -0.3246018864830802 1104 92-93 -0.1561351241611888 1104 94-95 -0.24378299298524553 1104 96-97 -0.18897107015470027 1104 98-99 -0.12231997061663691 1104 100-101 0.08847980828611668 1105 1 -0.02490306765714223 1105 2 -0.042751201881451095 1105 3 -0.05614733128423666 1105 4 0.06508601319232099 1105 5 -0.11760246584734091 1105 6 -0.1154950470186904 1105 7 -0.08421958579432243 1105 8 -0.08931128338298322 1105 9 -0.06774129039983023 1105 10-11 -0.0711539579919247 1105 12-13 -0.0018481264977836531 1105 14-15 -0.029194443844950513 1105 16-17 -0.05644474976805469 1105 18-19 -0.1203311329775687 1105 20-21 -0.2065407298919979 1105 22-23 -0.18950371888738715 1105 24-25 -0.13842858982578576 1105 26-27 -0.3510374233476057 1105 28-29 -0.13210201048448766 1105 30-31 -0.03726636370560499 1105 32-33 -0.05431453708686007 1105 34-35 -0.10520623592034184 1105 36-37 -0.5212931700189998 1105 38-39 -0.4477666424685296 1105 40-41 -0.42216134099678726 1105 42-43 -0.3727829810642618 1105 44-45 -0.18029134792417523 1105 46-47 -0.41161505705784407 1105 48-49 -0.3204525072636528 1105 50-51 -0.39813702996303846 1105 52-53 -0.3103732776130741 1105 54-55 -0.21440231935302023 1105 56-57 -0.2901449730999133 1105 58-59 -0.19316201253617038 1105 60-61 -0.23880531063714727 1105 62-63 -0.44514255457882257 1105 64-65 -0.4789106358284556 1105 66-67 -0.4734940369548539 1105 68-69 -0.4045387959452924 1105 70-71 -0.46637505142110314 1105 72-73 -0.5458697550655955 1105 74-75 -0.5188146817616541 1105 76-77 -0.4791594881956627 1105 78-79 -0.3037060294274241 1105 80-81 -0.26996307462808744 1105 82-83 -0.0640202850533953 1105 84-85 0.056046074144823876 1105 86-87 -0.1431518000399521 1105 88-89 -0.3934681549716075 1105 90-91 -0.2391795982138163 1105 92-93 -0.08080890826558118 1105 94-95 -0.2552478033824386 1105 96-97 -0.4431131321259443 1105 98-99 -0.04524046649086699 1105 100-101 0.14253192461580966 1106 1 0.07520145415556101 1106 2 0.11879721411536082 1106 3 0.04452957188376416 1106 4 0.056077228382825695 1106 5 0.12274793108668547 1106 6 0.011847361462514527 1106 7 0.08439649563315044 1106 8 0.11071111414831591 1106 9 0.1609997194799746 1106 10-11 0.14360103790185974 1106 12-13 0.11553982363037818 1106 14-15 0.12510588216355956 1106 16-17 0.10959561556056485 1106 18-19 0.07393012178189906 1106 20-21 0.17790045170220736 1106 22-23 0.11486006679312766 1106 24-25 0.07995978336685283 1106 26-27 0.13800022939111045 1106 28-29 0.11987021657670738 1106 30-31 0.16150550499508398 1106 32-33 0.1646773251434368 1106 34-35 0.32434730662252065 1106 36-37 0.443007653712435 1106 38-39 0.4048016077865455 1106 40-41 0.2956919800593809 1106 42-43 0.24999904118395477 1106 44-45 0.2700732341129992 1106 46-47 0.20496409332913856 1106 48-49 0.23505604112745715 1106 50-51 0.39275090772206056 1106 52-53 0.17451461029972393 1106 54-55 0.3325827240733048 1106 56-57 0.42369545693268407 1106 58-59 0.4035828795823946 1106 60-61 0.2038943384681957 1106 62-63 0.16848758752107074 1106 64-65 0.1094476251276717 1106 66-67 0.11225740022006647 1106 68-69 0.08751008044522024 1106 70-71 0.29491081573664957 1106 72-73 0.483184820252319 1106 74-75 0.3818460454115282 1106 76-77 0.2991111735269527 1106 78-79 0.30991789113600277 1106 80-81 0.21349385989646663 1106 82-83 0.30326189895476574 1106 84-85 0.3904723240515082 1106 86-87 0.09059754669871722 1106 88-89 -0.19827366974153193 1106 90-91 0.1662406043594089 1106 92-93 0.16496044693437995 1106 94-95 0.2840225348202807 1106 96-97 0.3545687233850927 1106 98-99 0.13667103837437367 1106 100-101 0.12211284191914729 1107 1 -0.14926455602473254 1107 2 -0.05524303135345576 1107 3 0.02297969319103288 1107 4 0.009647467667349474 1107 5 0.12091642925518187 1107 6 0.08957915348001677 1107 7 0.08170870723107271 1107 8 0.12067733840025596 1107 9 0.11012202574513452 1107 10-11 0.12881820169045 1107 12-13 0.0777086072277342 1107 14-15 0.07433522425004213 1107 16-17 0.04072401097467804 1107 18-19 0.08120855763176849 1107 20-21 0.1192091430108988 1107 22-23 0.14901638666373174 1107 24-25 0.14998499624920925 1107 26-27 0.1623925037833871 1107 28-29 0.2381923706131417 1107 30-31 0.2675185395795481 1107 32-33 0.40705399609153403 1107 34-35 0.1074332682799124 1107 36-37 0.2452173487792706 1107 38-39 0.23750461906098508 1107 40-41 0.24517107239561398 1107 42-43 0.369736446635649 1107 44-45 0.4761409758950279 1107 46-47 0.2594334335127684 1107 48-49 0.24647319540175516 1107 50-51 0.3511972233112317 1107 52-53 0.2796356797065087 1107 54-55 0.36497889932662275 1107 56-57 0.32111946149954207 1107 58-59 0.437584592155531 1107 60-61 0.37987312158983855 1107 62-63 0.5541614374806372 1107 64-65 0.4602159044673826 1107 66-67 0.33897116264811444 1107 68-69 0.4559630560410497 1107 70-71 0.5087683867370814 1107 72-73 0.41026964019428647 1107 74-75 0.4105435383947338 1107 76-77 0.30424889295038327 1107 78-79 0.4006842676166613 1107 80-81 0.6820728999040782 1107 82-83 0.5921396878325638 1107 84-85 0.5961119225482463 1107 86-87 0.4929570919154074 1107 88-89 0.5774387131137004 1107 90-91 0.7107199345530262 1107 92-93 0.5224363044102347 1107 94-95 0.18897472548675154 1107 96-97 0.3213638330373456 1107 98-99 0.45839693152883854 1107 100-101 0.6065331834823482 1108 1 -0.29892441997031227 1108 2 -0.17849759633734408 1108 3 -0.2441389963900633 1108 4 -0.09758489896351108 1108 5 -0.013638708589425619 1108 6 -0.19729875557834475 1108 7 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0.3484255244392429 2316 68-69 0.25156180598186495 2316 70-71 0.23625311612767774 2316 72-73 0.3179081016113301 2316 74-75 0.16139190558383376 2316 76-77 0.26808477533117525 2316 78-79 0.09743837529221366 2316 80-81 0.19537450825043123 2316 82-83 0.39834894647801633 2316 84-85 0.2506568031454961 2316 86-87 0.2189581651985506 2316 88-89 0.24172600575698056 2316 90-91 0.29215529291911224 2316 92-93 0.06466920685195277 2316 94-95 0.2689737784722226 2316 96-97 0.04206162491511378 2316 98-99 -0.3415265181294487 2316 100-101 -0.40084563811590357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 6.0 15 24.0 16 51.0 17 140.0 18 309.0 19 545.0 20 853.0 21 1274.0 22 1931.0 23 1988.0 24 1526.0 25 1349.0 26 941.0 27 954.0 28 1077.0 29 1196.0 30 1244.0 31 1497.0 32 1838.0 33 2478.0 34 3540.0 35 6046.0 36 11920.0 37 40575.0 38 126745.0 39 78906.0 40 141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22242344703882 21.888104381429912 14.707033211328765 22.182438960202504 2 14.256015386105462 22.844284864333844 42.08279717183457 20.816902577726122 3 16.26697106487487 29.282761721925322 33.28075546100763 21.169511752192186 4 10.911292135803109 19.87997025199329 42.298206471921 26.910531140282608 5 10.924512181201205 40.366940742252496 36.8839785121051 11.8245685644412 6 25.87350179006901 41.304069596499424 20.425811584427265 12.396617029004306 7 23.01596361057784 35.55440253203964 25.541085110430828 15.888548746951695 8 23.13460973036545 36.408101143222815 23.195835279060518 17.261453847351216 9 25.52863245645895 16.3043290267905 23.021152216399454 35.1458863003511 10-11 21.69805773188744 28.214773690309414 31.86461197876131 18.222556599041837 12-13 23.147235337864718 27.21614002317577 30.50779155640879 19.12883308255072 14-15 20.81720541690448 28.28551168301077 29.00015565817465 21.8971272419101 16-17 19.17120669676058 31.130770162057452 29.64596412943842 20.052059011743545 18-19 18.927697964916167 29.795142504298134 32.352401938570424 18.924757592215276 20-21 20.637701714028747 28.850684054862757 31.788228375737237 18.72338585537126 22-23 20.298517788270292 29.22862726785313 31.111572320517478 19.361282623359102 24-25 19.750428059980283 29.286047838945677 31.559349002922914 19.40417509815113 26-27 19.76453577364717 29.2206365870659 31.641348583565243 19.373479055721692 28-29 19.373389370276207 29.171898510870133 32.13286289973884 19.321849219114824 30-31 20.176931458517096 29.05550078693855 31.580103426209376 19.187464328334976 32-33 19.89052041716391 29.10548435635345 31.52268285511683 19.481312371365814 34-35 19.952783687023295 29.26062367041976 31.532195299123128 19.25439734343382 36-37 20.027984800344534 28.976375501365514 31.209797793386386 19.785841904903567 38-39 19.536025237902514 29.414643933183203 31.622268574729585 19.427062254184698 40-41 19.803870699942923 28.964527231532887 31.691831404901507 19.539770663622686 42-43 20.165689479236928 28.889984261229007 31.024749649769106 19.919576609764956 44-45 20.249398986492327 29.193690655320914 30.798180528891887 19.758729829294868 46-47 20.087221421664196 29.194784705632028 30.558289754337288 20.15970411836649 48-49 19.9018303893404 29.342958890178366 30.7409591772847 20.014251543196533 50-51 20.11449523513032 29.41766547328733 30.106885279925283 20.360954011657068 52-53 20.277763364983826 29.635413964267805 30.242826752451617 19.843995918296752 54-55 20.585966550787806 29.327902592573373 29.80992407340148 20.276206783237345 56-57 20.508137463463566 29.696293605908092 29.68591639426486 20.10965253636348 58-59 20.186962763105555 29.672944879710823 30.069873225064427 20.0702191321192 60-61 20.637981759595412 29.83895656445283 29.72687822030659 19.79618345564517 62-63 20.228843243243244 29.660713513513514 30.152475675675678 19.957967567567568 64-65 20.185322281570865 29.43517698502754 30.062719674535742 20.316781058865857 66-67 20.132592595154993 29.795801176832793 29.715892777456837 20.355713450555378 68-69 20.382861847339544 29.669890620784127 29.760694049521597 20.186553482354732 70-71 20.29820956650478 29.24121041052675 30.249366983520815 20.21121303944765 72-73 20.34041655280244 29.288068989612125 30.014147990051438 20.357366467533993 74-75 20.299498241857457 29.545788673514746 29.44028282485856 20.714430259769237 76-77 19.992424360351386 29.607779040384386 29.911842385184954 20.487954214079274 78-79 20.107058233452673 29.72898182258427 29.60964388868711 20.554316055275947 80-81 20.24888012591017 29.649596153513553 29.561908715128244 20.539615005448038 82-83 20.48461578373891 29.645099361801485 29.53337138310936 20.33691347135025 84-85 20.420104118023488 29.295906190006743 29.622961310295924 20.661028381673844 86-87 19.973122882389262 29.706824954556225 29.70820858995446 20.611843573100053 88-89 20.414061486574134 29.355299001167467 29.604012625935056 20.626626886323347 90-91 20.120514851622143 29.472003818840303 30.032895988488136 20.37458534104942 92-93 20.409553952852868 29.53510091838323 29.76737750566423 20.28796762309967 94-95 20.19742349709763 29.467887115409898 29.649674477815367 20.685014909677108 96-97 20.390529064840276 29.56242757571041 29.548937200574205 20.49810615887511 98-99 20.17537487677061 29.644407547691937 29.542883827115652 20.6373337484218 100-101 20.12936923848562 29.736764731316693 29.607568446358464 20.526297583839224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162.0 1 96.5 2 39.0 3 47.0 4 40.0 5 36.0 6 45.5 7 90.5 8 169.0 9 225.5 10 193.5 11 146.5 12 164.0 13 170.5 14 175.0 15 217.5 16 224.5 17 236.5 18 312.0 19 358.0 20 405.0 21 488.5 22 567.0 23 701.0 24 884.5 25 1190.5 26 1685.5 27 2495.5 28 3523.0 29 5059.5 30 6844.0 31 8185.5 32 9853.5 33 11789.5 34 13467.0 35 14626.0 36 15497.0 37 15596.0 38 14263.5 39 12911.5 40 11788.5 41 10832.0 42 9983.5 43 9316.0 44 9115.5 45 8992.0 46 8675.0 47 8257.5 48 7920.0 49 7649.0 50 7196.5 51 6441.0 52 5950.5 53 5559.5 54 4922.5 55 4281.0 56 3730.5 57 3297.5 58 2892.0 59 2418.5 60 1975.5 61 1491.5 62 1067.5 63 714.5 64 459.5 65 302.5 66 203.0 67 165.5 68 126.0 69 89.0 70 66.0 71 48.5 72 29.5 73 18.5 74 11.0 75 5.0 76 1.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10653937287050969 2 0.0010377211643231464 3 0.0 4 0.0 5 6.918141095487643E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.005534512876390114 20-21 0.003459070547743821 22-23 0.0 24-25 0.0 26-27 0.002248395856033484 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0032861170203566303 38-39 0.0020754423286462927 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.020927376813850117 48-49 0.0012106746917103376 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.003977931129905394 62-63 0.011241979280167418 64-65 0.010723118698005846 66-67 0.00484269876684135 68-69 0.003113163492969439 70-71 0.0010377211643231464 72-73 0.002767256438195057 74-75 0.003977931129905394 76-77 0.0036320240751310123 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 5.188605821615732E-4 88-89 0.002594302910807866 90-91 6.918141095487643E-4 92-93 0.0 94-95 0.0072640481502620246 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 289095.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.695585931516966 #Duplication Level Percentage of deduplicated Percentage of total 1 72.24879883643406 40.961879828799304 2 15.288710995442889 17.336048560485214 3 5.564705479987503 9.464827131726445 4 2.5568061090323786 5.7983848185949105 5 1.3441395042051238 3.8103388382304106 6 0.7973201930154862 2.7122721310825915 7 0.49370603566681537 1.9593667079039554 8 0.34177425745441903 1.5501673426149933 9 0.2359627850284634 1.204024351969918 >10 1.0400816040086556 10.363822930930345 >50 0.07076456621174804 2.62457095397914 >100 0.01599894540439521 1.4481714781293913 >500 6.153440540152004E-4 0.34608193358331873 >1k 6.153440540152004E-4 0.4200429919700764 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1204 0.41647209394835605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 992 0.3431397983361871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.17260762033241667 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 311 0.10757709403483284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.647676369359554E-4 0.0 18-19 0.0 0.0 0.0 0.0010377211643231464 0.0 20-21 0.0 0.0 0.0 0.0010377211643231464 0.0 22-23 0.0 0.0 0.0 0.0019024888012591015 0.0 24-25 0.0 0.0 0.0 0.002767256438195057 0.0 26-27 0.0 0.0 0.0 0.003113163492969439 0.0 28-29 0.0 0.0 0.0 0.004323838184679777 0.0 30-31 0.0 0.0 0.0 0.009339490478908318 0.0 32-33 0.0 0.0 0.0 0.015219910410072814 0.0 34-35 0.0 0.0 0.0 0.023175772669883604 0.0 36-37 0.0 0.0 0.0 0.039087497189505174 0.0 38-39 0.0 0.0 0.0 0.05811238520209619 0.0 40-41 0.0 0.0 0.0 0.0774831802694616 0.0 42-43 0.0 0.0 0.0 0.09460557948079351 0.0 44-45 0.0 0.0 0.0 0.11743544509590273 0.0 46-47 0.0 0.0 0.0 0.1416489389301095 0.0 48-49 0.0 0.0 0.0 0.16551652570954184 0.0 50-51 0.0 0.0 0.0 0.19059478718068457 0.0 52-53 0.0 0.0 0.0 0.21204102457669624 0.0 54-55 0.0 0.0 0.0 0.23296840139054636 0.0 56-57 0.0 0.0 0.0 0.26600252512149986 0.0 58-59 0.0 0.0 0.0 0.2983448347429046 0.0 60-61 0.0 0.0 0.0 0.32601739912485517 0.0 62-63 0.0 0.0 0.0 0.3564572199450008 0.0 64-65 0.0 0.0 0.0 0.3891454366211799 0.0 66-67 0.0 0.0 0.0 0.42511977031771564 0.0 68-69 0.0 0.0 0.0 0.46524498867154396 0.0 70-71 0.0 0.0 0.0 0.5088292775731161 0.0 72-73 0.0 0.0 0.0 0.5465331465435237 0.0 74-75 0.0 0.0 0.0 0.5911551566094191 0.0 76-77 0.0 0.0 0.0 0.6468461924280946 0.0 78-79 0.0 0.0 0.0 0.7013265535550598 0.0 80-81 0.0 0.0 0.0 0.7559798682094121 0.0 82-83 0.0 0.0 0.0 0.8089036475898925 0.0 84-85 0.0 0.0 0.0 0.8730694062505404 0.0 86-87 0.0 0.0 0.0 0.944153306006676 0.0 88-89 0.0 0.0 0.0 1.0214635327487502 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 545 0.0 57.5806 1 GGTATAG 40 5.354921E-4 47.54762 1 GTATCAA 1095 0.0 46.896282 1 GTATAGA 85 3.5997073E-7 39.15686 1 TCAACGC 1275 0.0 38.37115 4 ATCAACG 1295 0.0 37.778545 3 CAACGCA 1305 0.0 37.489056 5 TATCAAC 1315 0.0 37.203968 2 AACGCAG 1335 0.0 36.646606 6 ACGCAGA 1565 0.0 30.35033 7 CGCAGAG 1590 0.0 29.873127 8 GTACATG 1295 0.0 29.005884 1 CATGGGG 495 0.0 27.827269 4 TACATGG 1315 0.0 27.812675 2 GCAGAGT 1735 0.0 27.376526 9 ACATGGG 1250 0.0 27.359003 3 GACGAGA 35 0.008382595 27.146564 16-17 TATAGTT 125 7.367975E-6 26.599031 5 GTATAGT 90 8.933189E-4 26.387928 4 GAGTACT 1105 0.0 25.36326 12-13 >>END_MODULE