FastQCFastQC Report
Fri 10 Feb 2017
SRR2031465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737811
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA44550.6038131716659145No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT37180.50392309141501No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT17640.23908561948791765No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC10150.13756910645138118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.042.3939971
GTATCAA10250.040.7485921
TCAACGC9850.040.002224
CAACGCA9950.039.1230775
ATCAACG10250.038.441163
AACGCAG11000.035.3789946
TATCAAC12350.032.2889942
ACGCAGA12300.031.25397
CGCAGAG12450.030.8773468
GTACATG17600.026.6978531
ACATGGG16250.026.000333
TACTCGC950.001232229624.9787527
GCAGAGT16850.023.096089
TACATGG18800.022.9787182
ATACTCG1050.002206354222.5998236
GAGTACA13100.022.4633311
GGTTCGT550.002863628621.60921578-79
ACCGTGC1353.8160087E-421.0931688
ACACCGT1605.018929E-520.7635886
CATGGGG10550.020.248944
Produced by FastQC (version 0.11.5)