FastQCFastQC Report
Fri 10 Feb 2017
SRR2031465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737811
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA46800.6343087863965161No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT34100.462177983250453No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT17320.23474846539289873No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC10080.1366203539930958No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT9420.1276749736721193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.041.3017851
GTATCAA11100.038.1144641
TCAACGC11300.037.4068874
CAACGCA11600.036.439475
ATCAACG11650.035.87543
AACGCAG12400.034.4715546
TATCAAC13250.032.2623562
CGCAGAG13750.029.3600128
ACGCAGA14200.029.0985227
ACATGGG17800.026.68213
GTACATG19650.025.884751
TACATGG20400.023.9815082
GCAGAGT16700.023.3204759
CATGGGG13250.021.8652254
CCGTTAA1100.002878461521.5882438
ACCGTTA1157.9906714E-420.6496247
TAATACT7700.020.3546314
AGAGTAC15100.019.81543510-11
GAGTACT8750.019.54176912-13
GAGTACA12650.019.5404971
Produced by FastQC (version 0.11.5)