FastQCFastQC Report
Fri 10 Feb 2017
SRR2031464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261352
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.048.4378431
GTATCAA19600.043.3338171
TCAACGC20100.040.8427054
ATCAACG20450.040.1436843
CAACGCA20650.039.525095
AACGCAG21700.037.597666
CGCAGAG24450.033.7568978
TATCAAC24600.033.371482
ACGCAGA24600.033.358247
ACATGGG30900.025.6461643
GCAGAGT31450.025.34059
CATGGGG18100.025.1684884
GTACATG32850.024.8440861
TACATGG35550.022.8255352
GTATATA13150.020.9282151
GTAGACG704.864859E-420.35036368-69
ATGGGGA9800.019.8528215
TGGACCG1200.004791057719.772125
CAGAGTA31100.019.67686510-11
AGAGTAC29450.018.84634410-11
Produced by FastQC (version 0.11.5)