FastQCFastQC Report
Fri 10 Feb 2017
SRR2031464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261352
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.048.4607121
GTATCAA19100.040.5612071
TCAACGC20950.036.0476534
CAACGCA21500.035.1255045
ATCAACG21900.034.4839443
AACGCAG22450.033.6391226
ACGCAGA25250.029.9088467
TATCAAC25600.029.6854692
CGCAGAG25950.029.1020538
CGTATAC700.00839467827.1413
GTACATG32100.023.6903551
GCAGAGT32400.023.601789
ACATGGG28850.023.5425823
CATGGGG16750.022.117894
TACATGG36100.020.5249122
GTATTAC3950.020.4554231
GTATATA13350.019.9371661
AGAGTAC31550.019.57076810-11
CAGAGTA31550.019.34495410-11
TAATACT12950.019.0720544
Produced by FastQC (version 0.11.5)