FastQCFastQC Report
Fri 10 Feb 2017
SRR2031463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28982
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.7073355876061003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.6555793250983369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.638327237595749No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.5141122075771168No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC1130.3898971775584846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.3139879925470982No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC900.31053757504658064No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA760.26223173003933475No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC750.2587813125388172No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG710.24497964253674692No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA690.2380788075357118No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC660.22772755503415915No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG620.2139258850320889No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA610.2104754675315713No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC550.18977296252846595No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT510.17597129252639568No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC510.17597129252639568No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT490.16907045752536057No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT490.16907045752536057No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC470.16216962252432543No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA470.16216962252432543No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA470.16216962252432543No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT460.15871920502380787No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT460.15871920502380787No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC460.15871920502380787No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT460.15871920502380787No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA460.15871920502380787No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA460.15871920502380787No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA460.15871920502380787No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC450.15526878752329032No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG450.15526878752329032No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA440.15181837002277276No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA430.1483679525222552No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC430.1483679525222552No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT420.14491753502173765No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT410.14146711752122007No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT410.14146711752122007No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA400.1380167000207025No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT400.1380167000207025No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT390.13456628252018493No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG390.13456628252018493No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT390.13456628252018493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.13111586501966738No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT370.12766544751914982No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA370.12766544751914982No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG350.12076461251811468No Hit
AATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTAT340.11731419501759713No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC340.11731419501759713No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA340.11731419501759713No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG330.11386377751707957No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT330.11386377751707957No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT320.110413360016562No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC310.10696294251604445No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC300.10351252501552687No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA300.10351252501552687No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC290.10006210751500931No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT290.10006210751500931No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA290.10006210751500931No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA290.10006210751500931No Hit
GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT290.10006210751500931No Hit
GGAAAGATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTT290.10006210751500931No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT290.10006210751500931No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT290.10006210751500931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1550.055.1030241
ATAGGTG405.328628E-447.4498254
ATTGATG205.3734874E-447.44982546-47
TAGGTGG405.328628E-447.4498255
AGGTGGA459.519297E-442.1776246
GGTGGAT459.519297E-442.1776247
TATAGGT459.519297E-442.1776243
GTATCAA2050.041.663262
TATCAAC2050.041.663263
TCAACGC2150.039.7254375
ATCAACG2150.039.7254374
TTATAGG500.001598159437.9598662
AACGCAG2400.035.5873687
CAACGCA2400.035.5873686
AGAACTA1051.8333812E-631.6332195
ATAGCCT600.003908271431.6332175
ACGCAGA2800.030.503468
CGCAGAG2750.029.3326199
GAACTAG1153.7266236E-628.8825046
CTAGTAC1205.1901607E-627.6790649
Produced by FastQC (version 0.11.5)