##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031463_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28982 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.249775722862466 34.0 33.0 34.0 31.0 34.0 2 33.364950659029745 34.0 33.0 34.0 31.0 34.0 3 33.42447036091367 34.0 34.0 34.0 31.0 34.0 4 36.6326685528949 37.0 37.0 37.0 35.0 37.0 5 36.57508108481126 37.0 37.0 37.0 35.0 37.0 6 36.637188599820576 37.0 37.0 37.0 36.0 37.0 7 36.64008695052101 37.0 37.0 37.0 36.0 37.0 8 36.64819543164723 37.0 37.0 37.0 36.0 37.0 9 38.56493685735974 39.0 39.0 39.0 38.0 39.0 10-11 38.55995100407149 39.0 39.0 39.0 37.5 39.0 12-13 38.55970947484646 39.0 39.0 39.0 38.0 39.0 14-15 40.18878959354082 41.0 40.0 41.0 38.0 41.0 16-17 40.23026361189704 41.0 40.0 41.0 38.5 41.0 18-19 40.30698364502105 41.0 40.5 41.0 39.0 41.0 20-21 40.29704644261956 41.0 41.0 41.0 39.0 41.0 22-23 40.216686219032496 41.0 40.5 41.0 39.0 41.0 24-25 40.19160168380374 41.0 40.0 41.0 38.0 41.0 26-27 40.09850941963978 41.0 40.0 41.0 38.0 41.0 28-29 40.00039679801256 41.0 40.0 41.0 38.0 41.0 30-31 39.810365054171555 41.0 40.0 41.0 37.0 41.0 32-33 39.60846387412877 41.0 40.0 41.0 35.5 41.0 34-35 39.484818162997726 41.0 40.0 41.0 35.0 41.0 36-37 39.38761990200814 41.0 40.0 41.0 35.0 41.0 38-39 39.22629563177145 41.0 40.0 41.0 35.0 41.0 40-41 39.15759781933614 41.0 40.0 41.0 35.0 41.0 42-43 38.96304602856945 41.0 39.5 41.0 35.0 41.0 44-45 38.82452901801118 41.0 39.0 41.0 35.0 41.0 46-47 38.61910841211787 41.0 39.0 41.0 35.0 41.0 48-49 38.36897039541785 41.0 39.0 41.0 35.0 41.0 50-51 38.228072596784216 41.0 39.0 41.0 35.0 41.0 52-53 38.24180525843627 41.0 39.0 41.0 35.0 41.0 54-55 38.21242495341936 41.0 39.0 41.0 35.0 41.0 56-57 38.16361879787454 41.0 39.0 41.0 35.0 41.0 58-59 38.14415844317163 41.0 39.0 41.0 35.0 41.0 60-61 38.017735145952656 41.0 39.0 41.0 35.0 41.0 62-63 37.80425781519564 41.0 38.5 41.0 35.0 41.0 64-65 37.570647298323095 40.0 37.5 41.0 34.0 41.0 66-67 37.245359188461805 40.0 37.0 41.0 34.0 41.0 68-69 36.839624594575945 39.0 36.5 41.0 33.5 41.0 70-71 36.45831895659375 39.0 36.0 41.0 33.0 41.0 72-73 36.07756538541163 38.5 36.0 40.5 33.0 41.0 74-75 35.65375060382306 37.0 35.0 39.5 32.0 41.0 76-77 34.47817610930923 36.0 34.5 39.0 30.0 39.5 78-79 34.8106410875716 36.0 35.0 39.0 31.0 40.0 80-81 34.530432682354565 36.0 35.0 37.5 31.0 39.0 82-83 34.16674142571251 35.5 35.0 37.0 31.0 39.0 84-85 33.79130149748119 35.0 35.0 37.0 31.0 39.0 86-87 33.5546546132082 35.0 35.0 36.0 31.0 37.0 88-89 33.31119315437168 35.0 35.0 36.0 30.5 37.0 90-91 33.138258229245736 35.0 35.0 36.0 30.0 37.0 92-93 33.01742460837761 35.0 35.0 36.0 30.0 36.0 94-95 32.86524394451729 35.0 35.0 35.0 29.0 36.0 96-97 32.7473431785246 35.0 35.0 35.0 29.0 36.0 98-99 32.70443723690567 35.0 35.0 35.0 29.0 36.0 100-101 32.49660133876199 35.0 34.5 35.0 28.0 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44588374647345574 1101 2 0.3282852039376465 1101 3 0.38584969168242367 1101 4 0.12621881039720506 1101 5 0.34074504559313823 1101 6 0.22190165379026894 1101 7 0.14187715079316376 1101 8 0.2019973662191319 1101 9 0.1169226442324316 1101 10-11 0.036916546856680554 1101 12-13 0.20269594129324275 1101 14-15 0.39489481104728696 1101 16-17 0.42315731104446286 1101 18-19 0.26785701096670067 1101 20-21 0.2669193345672909 1101 22-23 0.4586985833648143 1101 24-25 0.33454099780859536 1101 26-27 0.03588990578350604 1101 28-29 0.32317346429251614 1101 30-31 0.5066207701827992 1101 32-33 0.37043654209079335 1101 34-35 0.20281350125296882 1101 36-37 0.6520925921821714 1101 38-39 0.7417565853953718 1101 40-41 0.07045515866771268 1101 42-43 0.822347717040536 1101 44-45 -0.06568142685608791 1101 46-47 0.1746137021036276 1101 48-49 0.5940817251389419 1101 50-51 0.5471014822394196 1101 52-53 1.0735940683359217 1101 54-55 0.4610217720982064 1101 56-57 0.5522672428351498 1101 58-59 1.0140762457901644 1101 60-61 1.2251454409201443 1101 62-63 0.9789240319173587 1101 64-65 0.6000169810946971 1101 66-67 0.8391851214162358 1101 68-69 0.5388306922907091 1101 70-71 0.6166136720885262 1101 72-73 -0.20300346968262062 1101 74-75 0.2010899033388327 1101 76-77 -0.040374864732690696 1101 78-79 0.29676451827410943 1101 80-81 1.2567012295356506 1101 82-83 1.255715857367207 1101 84-85 1.3541106639154563 1101 86-87 1.8438286850680825 1101 88-89 1.1598706774220915 1101 90-91 0.3635086597195283 1101 92-93 -7.638602787594095E-4 1101 94-95 0.6592245886027399 1101 96-97 0.46032366329693986 1101 98-99 0.37997135114801495 1101 100-101 1.1561933120591874 1102 1 0.12983023476776623 1102 2 -0.02121312382155338 1102 3 0.36745504285298836 1102 4 0.15631914484536225 1102 5 0.2738554134861175 1102 6 0.19347356014478834 1102 7 0.23886711734834165 1102 8 0.09664619565057109 1102 9 0.11357816262707843 1102 10-11 0.23591320237507318 1102 12-13 0.32644176069123887 1102 14-15 0.524493473254644 1102 16-17 0.23503022074346092 1102 18-19 -0.006390480672088472 1102 20-21 0.1415012743666253 1102 22-23 -0.12156897516361909 1102 24-25 0.271831967708259 1102 26-27 0.09525445427848922 1102 28-29 0.2805313238242917 1102 30-31 0.6805338136610644 1102 32-33 0.6588980805523335 1102 34-35 0.13926835075129418 1102 36-37 0.12282837813534542 1102 38-39 0.46750909375658267 1102 40-41 -0.18539768414165536 1102 42-43 -0.18601348697283981 1102 44-45 -0.27303928638786346 1102 46-47 0.022439789060150872 1102 48-49 0.9962556381824257 1102 50-51 1.4618172013029636 1102 52-53 1.312724503118531 1102 54-55 0.9677107353089127 1102 56-57 2.24123045353749 1102 58-59 1.6361498243854768 1102 60-61 1.615613668344892 1102 62-63 1.417887242619699 1102 64-65 2.2388129677167754 1102 66-67 2.007245322085133 1102 68-69 2.145017983260608 1102 70-71 1.3540718660684519 1102 72-73 1.7275985370063367 1102 74-75 1.369986224409061 1102 76-77 1.0750097506519225 1102 78-79 1.4138213744613992 1102 80-81 1.3177380188333103 1102 82-83 1.6980235496749003 1102 84-85 1.6801976204371911 1102 86-87 1.5227584509543703 1102 88-89 1.3237502760843043 1102 90-91 1.6737093286158498 1102 92-93 1.6288348019285976 1102 94-95 1.7746092039873531 1102 96-97 1.6543035964073098 1102 98-99 1.721108474893839 1102 100-101 1.3334508371427987 1103 1 -0.48555438061684697 1103 2 -0.4442900468984803 1103 3 -0.44023726483931824 1103 4 -0.45906547053925095 1103 5 -0.2646061249754226 1103 6 -0.5757572090859853 1103 7 -0.6072867288054979 1103 8 -0.5571999581955822 1103 9 -0.5018064527575348 1103 10-11 -0.4756252591633867 1103 12-13 -0.13509670084722813 1103 14-15 0.3129550117161841 1103 16-17 0.3504148361280741 1103 18-19 0.14745567317405772 1103 20-21 0.4299628128281583 1103 22-23 0.22458487099023472 1103 24-25 -0.035860339984047584 1103 26-27 0.03756214658618262 1103 28-29 0.10745440074736479 1103 30-31 0.026687659814911058 1103 32-33 0.46659038824464005 1103 34-35 0.6200375815205277 1103 36-37 0.6035976089045718 1103 38-39 0.7944321706796558 1103 40-41 0.5838330850891111 1103 42-43 1.1409095899502333 1103 44-45 1.2461914828429101 1103 46-47 1.5416705582909174 1103 48-49 1.688563330490119 1103 50-51 1.4233556628414235 1103 52-53 1.4665706569646915 1103 54-55 1.5254030430012193 1103 56-57 2.0681535304605703 1103 58-59 2.251534439770097 1103 60-61 1.827152129883359 1103 62-63 1.744810319542779 1103 64-65 2.0657360446398485 1103 66-67 2.295706860546673 1103 68-69 1.9142487524913676 1103 70-71 1.8348410968376925 1103 72-73 1.9391369985448037 1103 74-75 2.0622939167167544 1103 76-77 2.094240519882696 1103 78-79 1.8561290667690926 1103 80-81 1.7985072496025367 1103 82-83 1.7749466265979805 1103 84-85 1.6994283896679647 1103 86-87 2.1766046048005236 1103 88-89 2.246827199161224 1103 90-91 2.0967862516927767 1103 92-93 1.9172963403901377 1103 94-95 2.2169168962950465 1103 96-97 2.01968821179193 1103 98-99 1.9903392441246126 1103 100-101 2.0449892986812657 1104 1 -0.10806150312397023 1104 2 0.11411451150608087 1104 3 0.0782812536792008 1104 4 -0.24254125401503046 1104 5 -0.27600213637143156 1104 6 0.19774706441828727 1104 7 0.1676420461232766 1104 8 0.2148798138841883 1104 9 0.31015935920827076 1104 10-11 0.16896163542350706 1104 12-13 0.1640485982980735 1104 14-15 0.5551202538814266 1104 16-17 0.38531512102836274 1104 18-19 0.2984528241712141 1104 20-21 0.36514799801334163 1104 22-23 0.31860196500732485 1104 24-25 0.01328495916125405 1104 26-27 -0.060728451704413544 1104 28-29 0.06471935801232576 1104 30-31 0.12497825810550722 1104 32-33 0.4259920976463505 1104 34-35 -0.10717039568745435 1104 36-37 -0.10153059622363259 1104 38-39 -0.26468463843715284 1104 40-41 -0.5913805901245581 1104 42-43 -0.6760419770013328 1104 44-45 -0.5479680613166309 1104 46-47 0.077995344615708 1104 48-49 0.4129223048490971 1104 50-51 1.1042673437531079 1104 52-53 1.1090207994148216 1104 54-55 0.8893631569613376 1104 56-57 0.7825409948480271 1104 58-59 0.9979731862088457 1104 60-61 0.7203145230457508 1104 62-63 0.19067926541171687 1104 64-65 0.6362488651526732 1104 66-67 0.7216327864725969 1104 68-69 0.9961575844002013 1104 70-71 1.1190291310257265 1104 72-73 0.6399916993995021 1104 74-75 0.9981913526141994 1104 76-77 0.7744399500821189 1104 78-79 1.4594054200454352 1104 80-81 0.8782793293746209 1104 82-83 0.4964565981079474 1104 84-85 0.7913087315483054 1104 86-87 1.0797385079344295 1104 88-89 0.8579383102723384 1104 90-91 0.7370996420061573 1104 92-93 1.1964986195924183 1104 94-95 0.7518171811953351 1104 96-97 0.6970386391423489 1104 98-99 0.5216782754636426 1104 100-101 -0.0853525816606222 1105 1 0.1141607190982512 1105 2 0.18818858558015705 1105 3 0.04124421664216271 1105 4 -0.24254125401503046 1105 5 0.2054793451100494 1105 6 0.19774706441828727 1105 7 0.1676420461232766 1105 8 -0.007342408338033124 1105 9 -0.09724804819913402 1105 10-11 0.15044311690498802 1105 12-13 0.18256711681659255 1105 14-15 -0.4819167831556044 1105 16-17 0.18161141732465325 1105 18-19 0.3725268982452903 1105 20-21 -0.005222372357025051 1105 22-23 -0.0332498868445299 1105 24-25 0.12439607027236121 1105 26-27 0.06890117792521266 1105 28-29 -0.4167621234691552 1105 30-31 -0.20835507522782137 1105 32-33 0.018584690238938606 1105 34-35 0.15208886357181228 1105 36-37 -0.7311602258532588 1105 38-39 -0.8943142680667862 1105 40-41 -1.3691583679023438 1105 42-43 -1.2315975325568829 1105 44-45 -1.529449542798119 1105 46-47 -1.2923750257546587 1105 48-49 -1.124114732187941 1105 50-51 -1.3957326562468921 1105 52-53 -1.224312533918507 1105 54-55 -1.6661923985942195 1105 56-57 -1.27301456070753 1105 58-59 -1.5761008878652305 1105 60-61 -0.7056114028801801 1105 62-63 -1.6982096234771689 1105 64-65 -0.9193066904028839 1105 66-67 -1.6302190653792508 1105 68-69 -2.651990563747944 1105 70-71 -2.2513412393446472 1105 72-73 -2.489637930230124 1105 74-75 -2.261067906645067 1105 76-77 -1.5218563462141717 1105 78-79 -1.7998538392138173 1105 80-81 -1.3254243743290814 1105 82-83 -0.2998396981883502 1105 84-85 -1.1531357128961446 1105 86-87 -1.67952075132483 1105 88-89 -1.0309505786165545 1105 90-91 -1.207344802438282 1105 92-93 -1.507205084111284 1105 94-95 -1.285219855841703 1105 96-97 -1.4881465460428345 1105 98-99 -1.5894328356474716 1105 100-101 -1.1223896186976532 1106 1 0.5638961688337005 1106 2 0.20406160145316932 1106 3 0.48833416373211236 1106 4 0.24423123275744985 1106 5 0.35627299590370143 1106 6 0.30885817552940154 1106 7 0.31579019427142185 1106 8 0.2889538879582645 1106 9 0.421270470319385 1106 10-11 0.3567923232541972 1106 12-13 0.40611209036156737 1106 14-15 0.494273693034863 1106 16-17 0.3820082427214757 1106 18-19 0.3672358929542767 1106 20-21 0.3942485271138736 1106 22-23 0.5130464094517677 1106 24-25 0.4517770226533173 1106 26-27 0.6158588498828905 1106 28-29 0.5607511040440656 1106 30-31 0.6626766708039256 1106 32-33 0.6025793992336546 1106 34-35 0.7354221969051409 1106 36-37 0.7794217847287541 1106 38-39 0.9661354673829479 1106 40-41 1.0673495686056 1106 42-43 1.2617887108293573 1106 44-45 0.9824552191066473 1106 46-47 0.12958264620301208 1106 48-49 0.710541352468141 1106 50-51 0.8079710474568103 1106 52-53 1.0091530745471005 1106 54-55 0.6916118342100148 1106 56-57 0.6959007832078115 1106 58-59 0.8160948793305352 1106 60-61 1.103251030982257 1106 62-63 1.1404147151471733 1106 64-65 0.9338679127717171 1106 66-67 1.295706860546673 1106 68-69 1.2507872140298346 1106 70-71 1.168632305628897 1106 72-73 1.1122139216217235 1106 74-75 1.1708104002332362 1106 76-77 1.2274822781244552 1106 78-79 1.8053048909449174 1106 80-81 1.7023534034486971 1106 82-83 1.0950015716529222 1106 84-85 0.948054763294337 1106 86-87 1.0612199894159104 1106 88-89 1.0146843420183629 1106 90-91 1.0638192187257403 1106 92-93 0.33075787885167784 1106 94-95 0.1592245886027399 1106 96-97 0.6117211788248937 1106 98-99 1.1936359474213134 1106 100-101 0.6507585294504921 1107 1 -0.05991335497581929 1107 2 0.0326330300245985 1107 3 0.02642940182735032 1107 4 0.2537550422812629 1107 5 -0.30563176600106345 1107 6 -0.19114182447059846 1107 7 -0.01754313906191385 1107 8 0.05562055462493021 1107 9 0.18793713698605075 1107 10-11 0.07822089468277227 1107 12-13 0.09182637607585775 1107 14-15 0.23474988351105708 1107 16-17 0.4260558617691004 1107 18-19 0.39104541676380933 1107 20-21 -0.2200371871718403 1107 22-23 0.246379742785102 1107 24-25 -0.02560392972763026 1107 26-27 -0.09961734059330496 1107 28-29 0.12027491356788289 1107 30-31 -0.31946618633893564 1107 32-33 -0.31289679124254377 1107 34-35 -0.4312444697615305 1107 36-37 -0.7348639295569583 1107 38-39 -0.38505500880752663 1107 40-41 -0.3302694790134524 1107 42-43 0.3332172822579267 1107 44-45 -0.9127828761314518 1107 46-47 -0.7608935442731806 1107 48-49 0.3295889715157543 1107 50-51 0.07463771412347597 1107 52-53 -0.7039421635481347 1107 54-55 -0.4310072134090319 1107 56-57 -0.8267182644112339 1107 58-59 0.05538059361624903 1107 60-61 0.5258700786013009 1107 62-63 0.2999385246709778 1107 64-65 0.2195821984860018 1107 66-67 -0.4376264727866612 1107 68-69 -0.42421278597016254 1107 70-71 0.42458468658127657 1107 72-73 0.32888058828839206 1107 74-75 -0.13514198071914052 1107 76-77 0.1262918019339736 1107 78-79 0.061257271897297016 1107 80-81 -0.01431326321797144 1107 82-83 0.13904919070053978 1107 84-85 -0.3364690462294746 1107 86-87 -0.4054466772507581 1107 88-89 -0.13650613417210877 1107 90-91 -0.2647522098456889 1107 92-93 -0.11090878781498503 1107 94-95 -0.3407754113972601 1107 96-97 -0.5251835830798655 1107 98-99 -0.46350690972155206 1107 100-101 -0.6242414705495065 1108 1 0.26230886724640357 1108 2 0.2622626596542261 1108 3 0.04124421664216271 1108 4 0.2389402274664505 1108 5 0.2054793451100494 1108 6 0.0866359533071801 1108 7 -0.09161721313598292 1108 8 0.17784277684715022 1108 9 -0.24539619634727927 1108 10-11 0.02081348727536181 1108 12-13 0.14553007977955446 1108 14-15 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-1.660521190915766 2316 94-95 -1.9686823881414455 2316 96-97 -2.156966528816305 2316 98-99 -1.896065049256432 2316 100-101 -1.753311237991369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 6.0 16 17.0 17 59.0 18 74.0 19 100.0 20 151.0 21 249.0 22 312.0 23 240.0 24 157.0 25 144.0 26 183.0 27 185.0 28 216.0 29 180.0 30 167.0 31 156.0 32 189.0 33 256.0 34 398.0 35 848.0 36 1125.0 37 3218.0 38 13442.0 39 6906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.490779749982735 26.80779059327302 15.702051246633056 20.9993784101112 2 13.577392864536609 25.077634393761645 42.19515561382927 19.149817127872474 3 13.863777517079567 32.06127941480919 36.881512663032225 17.193430405079013 4 10.016562004002484 22.586432958387963 44.60699744669105 22.790007590918503 5 8.953833413843075 42.657511558898626 37.68545994065282 10.70319508660548 6 21.002691325650407 45.47305223932096 20.830170450624525 12.694085984404113 7 19.936512317990477 38.48250638327237 28.086398454212958 13.494582844524187 8 21.982609895797392 37.04023186805603 24.2633358636395 16.713822372507074 9 24.684286798702644 17.42805879511421 25.33986612380098 32.547788282382164 10-11 19.39307156165896 30.139396867020913 33.40521703126078 17.062314540059347 12-13 21.232144089434822 30.315368159547308 31.319439652197918 17.13304809881996 14-15 19.843351045476503 30.9140155958871 29.616658615692497 19.625974742943896 16-17 16.76040300876406 34.309226416396385 30.386101718307916 18.54426885653164 18-19 16.877717203781657 32.11821130356773 34.02974259885446 16.97432889379615 20-21 17.611538594251407 31.372278389289534 34.170663538180186 16.845519478278874 22-23 18.426954661514046 31.474708439721205 32.80139396867021 17.29694293009454 24-25 18.337243806500584 32.399420329859915 33.01877027120281 16.244565592436686 26-27 17.12644273070752 32.14291875851836 34.67039318185739 16.06024532891672 28-29 17.51259402387689 30.546546132081982 35.00448554275067 16.936374301290456 30-31 18.435580705265338 31.267683389690156 33.258574287488784 17.038161617555726 32-33 18.199227106479885 31.136567524670482 34.74225381271134 15.921951556138293 34-35 17.73514595266027 31.66793181975019 34.095300531364295 16.501621696225243 36-37 18.534557123632727 31.016873123770743 32.65760325730651 17.790966495290018 38-39 17.11293606155757 32.126220627307546 33.64445671301887 17.11638659811601 40-41 16.496446069974464 31.38672279345801 35.37540542405631 16.741425712511212 42-43 17.18652957007798 31.10206334966531 33.43972120626596 18.271685873990755 44-45 17.7506728314126 31.089986888413495 33.531157270029674 17.628183010144227 46-47 18.66287094547964 30.80917874396135 33.08488612836439 17.443064182194618 48-49 17.357624691613616 31.349309041975054 33.10215137242724 18.190914893984093 50-51 17.08819267131323 30.9140155958871 32.81692084742254 19.18087088537713 52-53 17.79552825891933 31.938789593540818 32.98081567869712 17.28486646884273 54-55 18.13539438272031 31.195224622179285 31.73694016976054 18.932440825339867 56-57 18.597750327789665 32.630598302394596 30.81567869712235 17.955972672693395 58-59 17.06058933130909 31.89220895728383 32.3373128148506 18.709888896556485 60-61 19.002760524499653 32.43961352657005 31.283643892339548 17.273982056590754 62-63 17.67080745341615 33.15217391304348 31.937543133195305 17.239475500345065 64-65 17.6984126984127 32.91925465838509 31.44927536231884 17.933057280883368 66-67 18.13664596273292 33.35576259489303 30.46066252587992 18.046928916494135 68-69 18.03077878610124 32.50405438045616 31.180773610296402 18.2843932231462 70-71 17.763055742456395 32.11531494229077 32.78298224729569 17.338647067957144 72-73 18.592574701538886 31.673107446000966 31.588572217238287 18.14574563522186 74-75 17.944205786449977 32.30509117885548 30.440108344978693 19.310594689715856 76-77 17.524670485128702 32.92043337243806 32.03367607480505 17.52122006762818 78-79 17.918018080187704 31.899109792284868 31.160720447174107 19.02215168035332 80-81 17.88351390518253 31.954316472293147 31.321164860948176 18.841004761576148 82-83 18.042233110206336 33.01704506245255 30.891587882133738 18.04913394520737 84-85 17.560899868884135 31.85425436477814 30.996825615899525 19.588020150438204 86-87 16.981229728797185 32.37181698985577 32.30280863984542 18.34414464150162 88-89 17.82202132431593 32.14002277354129 31.691453020944753 18.346502881198028 90-91 17.655786350148368 31.98191981229729 31.999171899799876 18.363121937754467 92-93 18.30101442274515 32.53226140362984 31.366020288454905 17.800703885170105 94-95 18.064182194616976 31.323326432022085 31.892684610075918 18.719806763285025 96-97 18.94106686909116 30.68973845835346 31.32979090469947 19.039403767855912 98-99 17.705817403905872 31.885308122282797 31.17107169967566 19.23780277413567 100-101 17.49879235387482 32.49430681112415 31.938789593540818 18.068111241460215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 239.0 1 135.5 2 33.5 3 30.0 4 23.5 5 23.5 6 45.5 7 94.0 8 122.5 9 121.5 10 80.0 11 42.0 12 41.5 13 50.5 14 45.5 15 29.0 16 28.5 17 27.5 18 27.0 19 28.5 20 39.0 21 54.0 22 57.5 23 60.0 24 67.5 25 97.5 26 173.5 27 304.5 28 388.5 29 454.0 30 686.0 31 915.0 32 1233.5 33 1572.5 34 1838.0 35 2137.0 36 2459.5 37 2657.0 38 2265.0 39 1742.0 40 1403.5 41 1091.5 42 879.0 43 820.5 44 835.0 45 824.5 46 668.0 47 424.0 48 365.5 49 332.5 50 260.5 51 177.0 52 98.5 53 67.5 54 55.5 55 55.0 56 52.0 57 42.0 58 44.5 59 45.5 60 29.5 61 17.0 62 12.5 63 10.0 64 7.5 65 5.5 66 2.0 67 1.5 68 1.0 69 1.0 70 1.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0828100200124215 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0034504175005175626 22-23 0.0 24-25 0.0 26-27 0.0017252087502587813 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0034504175005175626 38-39 0.0034504175005175626 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.006900835001035125 48-49 0.0017252087502587813 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.006900835001035125 62-63 0.006900835001035125 64-65 0.006900835001035125 66-67 0.006900835001035125 68-69 0.0034504175005175626 70-71 0.0017252087502587813 72-73 0.0 74-75 0.0017252087502587813 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0034504175005175626 90-91 0.0 92-93 0.0 94-95 0.006900835001035125 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 28982.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.40721827341108 #Duplication Level Percentage of deduplicated Percentage of total 1 66.51781805446107 26.212821751431925 2 12.923561859732072 10.185632461527845 3 6.339199719814377 7.494306811124146 4 3.5548550914981174 5.603478020840521 5 2.1977059802118903 4.3302739631495415 6 1.6636021364153752 3.9334759505900214 7 1.208300499080641 3.3331033054999653 8 1.1119866911829086 3.5056241805258437 9 0.6566850538481744 2.3290318128493546 >10 3.6161456965239474 25.32261403629839 >50 0.14009281148761055 3.4538679180180805 >100 0.07004640574380527 4.295769788144366 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 223 0.7694431026154165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.6693809951004072 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 0.6348768200952315 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 157 0.5417155475812573 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.48995928507349384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.4623559450693534 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 110 0.3795459250569319 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 101 0.3484921675522738 RNA PCR Primer, Index 46 (95% over 21bp) ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 83 0.2863846525429577 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 78 0.26913256504036986 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 74 0.25533089503829964 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 67 0.2311779725346767 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 66 0.22772755503415915 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 65 0.2242771375336416 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 64 0.220826720033124 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 62 0.2139258850320889 RNA PCR Primer, Index 46 (95% over 23bp) GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 58 0.20012421503001862 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 58 0.20012421503001862 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 57 0.19667379752950107 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 56 0.1932233800289835 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 55 0.18977296252846595 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 54 0.1863225450279484 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 52 0.17942171002691326 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 52 0.17942171002691326 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 50 0.17252087502587812 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 49 0.16907045752536057 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 48 0.165620040024843 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 48 0.165620040024843 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 47 0.16216962252432543 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 46 0.15871920502380787 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 43 0.1483679525222552 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 43 0.1483679525222552 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 43 0.1483679525222552 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 43 0.1483679525222552 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 42 0.14491753502173765 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 41 0.14146711752122007 No Hit ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT 41 0.14146711752122007 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 39 0.13456628252018493 No Hit AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT 39 0.13456628252018493 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 39 0.13456628252018493 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 39 0.13456628252018493 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 37 0.12766544751914982 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 37 0.12766544751914982 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.12421503001863224 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 36 0.12421503001863224 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 35 0.12076461251811468 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 35 0.12076461251811468 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 35 0.12076461251811468 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 35 0.12076461251811468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35 0.12076461251811468 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 34 0.11731419501759713 RNA PCR Primer, Index 46 (96% over 28bp) CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 34 0.11731419501759713 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 34 0.11731419501759713 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 33 0.11386377751707957 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 33 0.11386377751707957 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 33 0.11386377751707957 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 32 0.110413360016562 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 32 0.110413360016562 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 31 0.10696294251604445 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 31 0.10696294251604445 No Hit ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG 31 0.10696294251604445 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 30 0.10351252501552687 No Hit ATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCG 30 0.10351252501552687 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.10351252501552687 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 29 0.10006210751500931 RNA PCR Primer, Index 46 (96% over 26bp) GTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAATAATTACATGGAGT 29 0.10006210751500931 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 29 0.10006210751500931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0034504175005175626 0.0 18-19 0.0 0.0 0.0 0.0034504175005175626 0.0 20-21 0.0 0.0 0.0 0.005175626250776344 0.0 22-23 0.0 0.0 0.0 0.010351252501552688 0.0 24-25 0.0 0.0 0.0 0.01380167000207025 0.0 26-27 0.0 0.0 0.0 0.017252087502587814 0.0 28-29 0.0 0.0 0.0 0.029328548754399282 0.0 30-31 0.0 0.0 0.0 0.04313021875646954 0.0 32-33 0.0 0.0 0.0 0.058657097508798564 0.0 34-35 0.0 0.0 0.0 0.09316127251397419 0.0 36-37 0.0 0.0 0.0 0.1397419087709613 0.0 38-39 0.0 0.0 0.0 0.1845973362776896 0.0 40-41 0.0 0.0 0.0 0.22600234628390037 0.0 42-43 0.0 0.0 0.0 0.26395693878959353 0.0 44-45 0.0 0.0 0.0 0.30363674004554553 0.0 46-47 0.0 0.0 0.0 0.346766958802015 0.0 48-49 0.0 0.0 0.0 0.3829963425574494 0.0 50-51 0.0 0.0 0.0 0.4002484300600373 0.0 52-53 0.0 0.0 0.0 0.41922572631288385 0.0 54-55 0.0 0.0 0.0 0.46753157132012974 0.0 56-57 0.0 0.0 0.0 0.5020357463253053 0.0 58-59 0.0 0.0 0.0 0.5365399213304809 0.0 60-61 0.0 0.0 0.0 0.5624180525843627 0.0 62-63 0.0 0.0 0.0 0.5951970188392796 0.0 64-65 0.0 0.0 0.0 0.6314264025947139 0.0 66-67 0.0 0.0 0.0 0.6814574563522187 0.0 68-69 0.0 0.0 0.0 0.7228624663584293 0.0 70-71 0.0 0.0 0.0 0.7780691463667104 0.0 72-73 0.0 0.0 0.0 0.8263749913739562 0.0 74-75 0.0 0.0 0.0 0.8781312538817196 0.0 76-77 0.0 0.0 0.0 0.9505900213925885 0.0 78-79 0.0 0.0 0.0 1.016147953902422 0.0 80-81 0.0 0.0 0.0 1.0851563039127736 0.0 82-83 0.0 0.0 0.0 1.130011731419502 0.0 84-85 0.0 0.0 0.0 1.2076461251811468 0.0 86-87 0.0 0.0 0.0 1.3094334414464148 0.0 88-89 0.0 0.0 0.0 1.3991442964598715 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAT 30 1.2800067E-4 63.333336 1 GTATCAA 215 0.0 39.767445 1 AACGCAG 230 0.0 39.239132 6 TTATAGT 50 0.0015899289 38.0 3 ACGCAGA 240 0.0 37.604168 7 CGCAGAG 240 0.0 37.604168 8 ATCAACG 250 0.0 36.1 3 CAACGCA 250 0.0 36.1 5 TCAACGC 255 0.0 35.39216 4 GCAGAGT 260 0.0 34.71154 9 TATCAAC 270 0.0 31.66667 2 ATCTCCT 30 0.003920309 31.666668 76-77 CCTTTTA 30 0.003920309 31.666668 86-87 CCTAGGC 30 0.003920309 31.666668 80-81 GGTATCA 120 1.4216494E-7 31.666668 1 AAATCTC 35 0.008327067 27.142859 74-75 CACATAC 35 0.008327067 27.142859 94-95 ACTTAAT 70 0.0082594985 27.142859 1 CATATTT 35 0.008327067 27.142859 56-57 TAATACT 90 8.7377743E-4 26.388887 4 >>END_MODULE