FastQCFastQC Report
Fri 10 Feb 2017
SRR2031462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766475
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.1313806712547702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.047.1778031
GTATCAA15350.045.4624251
TCAACGC16600.042.0417984
AACGCAG17400.040.9006426
CAACGCA17200.040.8512425
ATCAACG17700.039.6972543
TATCAAC19150.036.4411622
ACGCAGA20000.035.8207867
CGCAGAG20650.034.6932538
GTACATG22050.028.8496021
GCAGAGT25350.028.0738169
ACCCTCG700.00843761327.1112828
ACATGGG22550.026.3169463
TACATGG24550.024.9449042
CATGGGG15250.023.3487424
AGAGTAC21150.022.6568310-11
GTGTAAG4200.022.6060311
CAGAGTA23700.021.8205810-11
GAGTACT12650.021.37826512-13
ATATACC2551.1972588E-820.4797464
Produced by FastQC (version 0.11.5)