FastQCFastQC Report
Fri 10 Feb 2017
SRR2031462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766475
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.16530219511399588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.11194102873544473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.053.075381
GTATCAA15250.043.025861
TCAACGC17900.035.2888154
CAACGCA18050.034.732435
AACGCAG18350.033.9057736
ATCAACG18950.033.3334963
TATCAAC19750.032.2237552
CACGCGC404.4925246E-429.68953960-61
ACGCAGA21550.028.4302647
CGCAGAG21700.028.2337388
GTACATG21750.027.7628971
ACATGGG21950.025.7484383
TACATGG23050.025.1378042
CATGGGG16400.022.2989964
GCAGAGT27050.022.298469
CAATCGC652.943358E-421.92172248-49
AGAGTAC22850.020.88903210-11
TATACTG5400.020.228915
GTATTAC2651.8315404E-819.7363641
CATGGGA6750.019.70124
Produced by FastQC (version 0.11.5)