FastQCFastQC Report
Fri 10 Feb 2017
SRR2031459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185555
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.051.1198431
GTATCAA18500.036.4352231
TCAACGC19000.033.481974
ATCAACG19850.032.0482333
CAACGCA20300.031.8055325
AACGCAG20850.030.5021346
TACGACT650.005859536629.2150384
TATCAAC22750.028.3779242
ACGCAGA23700.026.6339027
CGCAGAG23950.026.3558858
GTACATG28600.023.2362921
TACATGG27800.022.3691582
ACATGGG27100.021.722623
ATACGTG1353.809863E-421.0997493
GCAGAGT31350.019.8327779
CATGGGG17600.019.421364
GTATATA11350.019.2383041
AGAGTAC26900.018.79008910-11
GAGTACA19750.018.0259861
GTATAAT9900.017.2612461
Produced by FastQC (version 0.11.5)