FastQCFastQC Report
Fri 10 Feb 2017
SRR2031459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185555
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10239929821897761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.042.9275631
GTATCAA18250.040.6257741
TCAACGC20000.036.094584
ATCAACG20500.035.44593
CAACGCA21100.034.2128755
AACGCAG22550.032.0129366
TATCAAC24700.029.4186612
ACGCAGA24800.029.1085367
CGCAGAG25050.028.6284398
GTACATG30200.027.5404511
ACATGGG28550.027.1150233
TACATGG29450.026.7701762
AGAGTAC26900.022.24573110-11
CATGGGG20350.021.704364
GCAGAGT33050.021.555019
AACCGTG1707.9633006E-519.555897
CAGAGTA33800.017.282919
CATGGGA13200.016.9103414
ATTATAC7800.016.439843
GAGTACT17100.016.38815712-13
Produced by FastQC (version 0.11.5)