FastQCFastQC Report
Fri 10 Feb 2017
SRR2031457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658805
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC41460.155934715031753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.041.686481
GTATCAA33950.039.5752141
TCAACGC33700.038.0402374
CAACGCA36050.035.6922045
ATCAACG37000.034.9041063
AACGCAG36800.034.82336
TATCAAC42200.030.7156352
ACGCAGA41850.030.5077937
CGCAGAG44650.028.4883528
GTACATG58550.027.4883981
ACATGGG59900.025.364863
CTTCGGC3550.022.7286979
ATACCGT1903.315199E-722.4824126
CATGGGG36000.022.421044
GCAGAGT58350.021.3115189
TACATGG76950.020.917052
TAATACT32350.020.4009254
TTAATAC36100.019.7284683
CTTAATA32650.018.1775842
AGAGTAC50300.017.88046610-11
Produced by FastQC (version 0.11.5)