FastQCFastQC Report
Fri 10 Feb 2017
SRR2031457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658805
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT51220.1926429354540856No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC46460.17474015582188238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21100.042.3584181
GTATCAA35750.036.4366841
TCAACGC35450.034.9687424
CAACGCA37350.033.3170435
ATCAACG37800.032.794763
AACGCAG38700.032.2775466
TATCAAC42950.029.5259482
GTACATG60500.028.6029191
ACGCAGA43750.028.1175527
ACATGGG60100.026.7114853
CGCAGAG46200.026.3180528
GCGTCCG950.00122760224.9978229
CATGGGG37350.023.9068874
TACATGG79900.021.3404452
TGGACCG2351.1343036E-720.2110065
ATGGGGC15750.019.9030285
GCAGAGT61700.019.4756139
TAATACT29900.019.3795834
TATAATA14300.018.9319172
AGAGTAC53250.017.83882110-11
Produced by FastQC (version 0.11.5)