FastQCFastQC Report
Fri 10 Feb 2017
SRR2031456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973699
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.1649380352655184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.11625769359935667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.057.524751
GTATCAA20000.040.5765231
TCAACGC22000.036.0304344
CAACGCA23150.034.035555
ATCAACG23350.033.7440263
AACGCAG23550.033.648626
GTGTACG902.427244E-531.638611
TATCAAC25650.030.9001162
ACGCAGA26700.029.8565587
CGCAGAG27400.028.7474448
ACATGGG28350.025.4487343
GTACATG30100.025.3844681
CTTCGGC1151.2906402E-424.7568349
TACATGG30750.024.077452
GCAGAGT33650.022.8439759
CATGGGG19300.022.3799844
AGAGTAC29550.020.71234510-11
GAGTACT19100.020.4935712-13
CAGAGTA33150.019.53647410-11
CATGGGA10500.018.0819954
Produced by FastQC (version 0.11.5)