FastQCFastQC Report
Fri 10 Feb 2017
SRR2031456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973699
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18380.18876470038482118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.1344357958671006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.050.2898371
GTATCAA20300.038.1779371
TCAACGC21250.035.9866564
ATCAACG21750.035.159383
CAACGCA22400.034.139135
AACGCAG22700.033.687956
ACGCAGA25550.029.5583937
TATCAAC26450.029.2709312
CGCAGAG26400.028.6067038
GTACATG26700.027.0678161
ACATGGG26400.024.288713
TACATGG28400.024.0834522
GCAGAGT32300.023.2342789
ATAGGAC3500.021.7133333
GTATAGG5350.021.3293861
CATGGGG18200.020.0952744
CTATAAC5500.019.8627663
AGAGTAC29200.019.68235610-11
GTATATA9200.019.6388761
CTCGTCC2953.0158844E-919.3211889
Produced by FastQC (version 0.11.5)