FastQCFastQC Report
Fri 10 Feb 2017
SRR2031454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945250
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16210.17148902406770697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.11340915101824914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.054.7961161
GTATCAA19800.044.5969921
TCAACGC23550.037.2959484
ATCAACG24150.036.3693433
CAACGCA24650.035.6316265
AACGCAG24900.035.449526
TATCAAC27050.032.4702262
ACGCAGA28000.031.3552637
CGCAGAG28750.030.7023668
GTACATG31200.029.823551
TACATGG31400.028.5767612
ACATGGG32050.027.5528013
CATGGGG20800.024.8796374
GCAGAGT35450.024.2303189
GTATTAC2853.6379788E-1223.320671
AGAGTAC30100.023.09765610-11
GAGTACT18250.021.4529912-13
CAGAGTA32950.020.95580510-11
GTATAAT6850.020.791631
GTATAGA5900.020.1161961
Produced by FastQC (version 0.11.5)