FastQCFastQC Report
Fri 10 Feb 2017
SRR2031454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945250
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.20851626553821742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.145781539275324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.058.644391
GTATCAA21850.046.5514451
TCAACGC25200.039.767754
CAACGCA26200.038.2498935
ATCAACG26200.038.0686153
AACGCAG26550.037.5667726
TATCAAC27850.035.983742
ACGCAGA30850.032.1766057
CGCAGAG31800.031.2153538
GTACATG31250.025.5523071
GCAGAGT38500.025.2896189
TACATGG32900.024.1084732
ACATGGG32350.023.0501883
AGAGTAC33700.021.6335410-11
CAGAGTA36750.020.61353310-11
CATGGGG21600.020.2294084
GAGTACT20900.020.225212-13
TTCGTCG600.004767440319.78963978-79
GTATATA6050.018.8550071
GTATTAT5800.018.0287461
Produced by FastQC (version 0.11.5)